http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors
정영미,Hyeong Kuyn Seo,Wonjin Hong 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.12
We introduced a promising patterned substrate by using a microcontact printing method that can be used for SERS immunoassays based on antigen-antibody binding. SERS spectrum of the Raman reporter with antibody,which is rhodamine 6G (R6G) adsorbed on colloidal gold nanoparticles, was observed only for the surfaces in which prostate-specific antigen (PSA) is present on the substrate that is attached to an immobilized layer of antibody on the gold nanoparticles layer of the patterned substrate. Raman mapping images clearly showed that the antibodies on the Raman reporter were successfully and selectively conjugated with the antigen on the patterned substrate. This method could be potentially extended to multi-protein detections and ultrasensitive biosensors.
Chen, Lei,Seo, Hyeong Kuyn,Mao, Zhu,Jung, Young Mee,Zhao, Bing Royal Society of Chemistry 2011 Analytical methods Vol.3 No.7
<P>We introduce a new approach that combines the self-assembly and seeding methods for silver plating of the proposed Fe<SUB>2</SUB>O<SUB>3</SUB>@Au-seed substrate. By adjusting the silver plating time between 0 and 360 s, the optical resonance of the substrates clearly varied from visible to near-infrared. To investigate the potential of these substrates for use in surface-enhanced Raman scattering (SERS) applications, SERS spectra of thiophenol (TP), 4-aminothiophenol (PATP) and rhodamine 6G (R6G) were evaluated at three excitation wavelengths (532, 633 and 785 nm). Analysis of the SERS spectra clearly demonstrated that the SERS effect depends strongly on the nature of the substrate surface and the nature and electronic resonance of the probe molecules. The calculated enhancement factor (EF) of TP adsorbed onto an Fe<SUB>2</SUB>O<SUB>3</SUB>@Ag substrate was ∼10<SUP>7</SUP> following three laser excitations. More importantly, these SERS substrates can successfully be utilized for the detection of small molecules at very low concentrations (∼100 pg mL<SUP>−1</SUP>).</P> <P>Graphic Abstract</P><P>The optimal Fe<SUB>2</SUB>O<SUB>3</SUB>@Ag substrate was employed for protein and small molecule recognition assay. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0ay00729c'> </P>
SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors
Hong, Won-Jin,Seo, Hyeong-Kuyn,Jung, Young-Mee Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.12
We introduced a promising patterned substrate by using a microcontact printing method that can be used for SERS immunoassays based on antigen-antibody binding. SERS spectrum of the Raman reporter with antibody, which is rhodamine 6G (R6G) adsorbed on colloidal gold nanoparticles, was observed only for the surfaces in which prostate-specific antigen (PSA) is present on the substrate that is attached to an immobilized layer of antibody on the gold nanoparticles layer of the patterned substrate. Raman mapping images clearly showed that the antibodies on the Raman reporter were successfully and selectively conjugated with the antigen on the patterned substrate. This method could be potentially extended to multi-protein detections and ultrasensitive biosensors.
Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen
Kyung Jin Yoon,Hyeong Kuyn Seo,황훈,표동진,엄인용,한종훈,정영미 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.5
We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.
Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen
Yoon, Kyung-Jin,Seo, Hyeong-Kuyn,Hwang, Hoon,Pyo, Dong-Jin,Eom, In-Yong,Hahn, Jong-Hoon,Jung, Young-Mee Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.5
We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.