A Partial Nucleotide Sequence of Chitin Synthase ( CHS ) Gene from Rice Blast Fungus , Pyricularia oryzae and Its Cloning

Cher Won Hwang,In Cheol Park,Won Hae Yeh,Masamchi Takagi,Jin Chang Ryu 한국미생물생명공학회 1997 Journal of microbiology and biotechnology Vol.7 No.2

일부 의사들의 직무스트레스와 관련 요인

강윤식,감신,이상원,천병렬,예민해 경북대학교 병원 2002 경북대학교병원의학연구소논문집 Vol.6 No.1

Odjective: To investigate the sources,extent and related factors in South Korean doctors. Methods: The study subjects were 934 doctors in Taegu,Kyungook Province,Korea(540 independent practitioners, 105 empolyed at hospitals and 289 residents in traning).Information concering job stress was obtained using a 9-item questionnaire.Information regarding related factors such as demographic characteristics(age, sex, marital status),perceptions on the socioeconomic status of doctors and working conditions(work time, on-call days per week)was also obtained by self-administered questionnaire during April and May,2000. Result: Major sources of jod stress included clnical responsibilty judgment factor,patient factor and work loading factor.The job stress score of residents was the highest among three groups.The score was lower in order doctors.The score was low among those who thought doctors'socioeconomic status was not good.The longer the work time, the higher the jod stress score was.Multiple regression analysis was conduted to control for the mutual influence of independent variables.In regression analysis, the score of residents was higher than practitioners. Work time and socioeconomic status perceotion had negative effects on job stress score. Conclusion: The average job stress score of the doctors was high. Age, work type, working conditions and perptions of socioeconomic status were found to besignificantly related to job stress score. Although the job stress of doctors is somewhat invitable due to the nature of the doctor's job, control of work time, development of coping tools and other intervention methods are needed to reduce job stress of doctors. Further studies are required to understand the characteristics of job stress and reduce the job stress of doctors.

벼 잎집무늬마름병의 발생정도에 따른 경제적 방제수준 설정

심홍식(Hongsik Shim),최효원(Hyo Won Choi),예완해(Wan-Hae Yeh),이용환(Yong-Hwan Lee) 한국농약과학회 2015 농약과학회지 Vol.19 No.4

Febry-Perot 간섭계를 이용한 광섬유 온도센서의 제조

김명규,박동수,김창원,예윤해,최시영,김기완,손병기 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

pBRG-4를 이용한 Metarhizium anisopliae의 형질전환

이동규,예완해,황철원,권석태,강선철,Lee, Dong-Gyu,Yeh, Wan-Hae,Hwang, Cher-Won,Kwon, Suk-Tae,Kang, Sun-Chul 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2

작물 병해충에 대한 무공해 미생물 농약 개발의 일환으로 곤충 병원성 곰팡이 Metarhizium anisopliae의 분자생물학적 육종을 위해 이 균주의 형질전환계를 확립하였다. M. anisopliae의 원형질체를 제작하여 benomyl 약제에 대하여 저항성을 나타내는 Aspergillus flavus 유래의 ${\beta}-tubulin$ 유전자를 갖는 pBRG-4 plasmid DNA를 polyethylene glycol 방법으로 형질 전환하였다. 이 형질전환은 pBRG-4 DNA $50\;{\mu}g$당 10개의 효율로 이루어졌으며, 그 결과 야생형 균주들의 $2.5\;{\mu}g/ml$ 농도의 benomyl 존재 하에서도 성장이 억제되는데 반하여 선발된 형질전환체들은 $5.0\;{\mu}g/ml$ 농도의 benomyl 함유 배지에서도 잘 성장하였다. 또한 이 형질전환체들의 chromosomal DNA를 분리하여 Southern 분석한 결과 ${\beta}-tubulin$ 유전자가 homologous recombination에 의하여 M. anisopliae의 genome속에 삽입 되었음을 확인하였다. We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a ${\beta}-tubulin$ gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per $50\;{\mu}g$ pBRG-4 DNA grew on the $5\;{\mu}g/ml$ concentrations of benamyl, while the wild type was inhibited by $2.5\;{\mu}g/ml$. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the ${\beta}-tubulin$ gene had integrated in the M. anisopliae genome by homologous recombination.

pBRG-4 를 이용한 Metarhizium anisopliae 의 형질전환

이동규,예완해,황철원,권석태,강선철 ( Dong Gyu Lee,Wan Hae Yeh,Cher Won Hwang,Suk Tae Kwon,Sun Chul Kang ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3

We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a β-tubulin gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per 50 ㎍ pBRG-4 DNA grew on the 5 ㎍/㎖ concentrations, of benamyl, while the wild type was inhibited by 2.5 ㎍/㎖. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the β-tubulin gene had integrated in the M. anisopliae genome by homologous recombination.

일부 의사들의 직무스트레스와 관련 요인

감신,이상원,천병렬,예민해,강윤식,Kam, Sin,Lee, Sang-Won,Chun, Byung-Yeol,Yeh, Min-Hae,Kang, Yune-Sik 대한예방의학회 2001 Journal of Preventive Medicine and Public Health Vol.34 No.2

Objective : To investigate the sources, extent and related factors in South Korean doctors. Methods : The study subjects were 934 doctors in Taegu, Kyungpook Province, Korea(540 independent practitioners, 105 employed at hospitals and 289 residents in training). Information concerning job stress was obtained using a 9-item questionnaire. Information regarding related factors such as demographic characteristics(age, sex, marital status), perceptions on the socioeconomic status of doctors and working conditions(work time, on-call days per week) was also obtained by self-administered questionnaire during April and May, 2000. Results : Major sources of job stress included clnical responsibility/judgement factor, patient factor and work loading factor. The job stress score of residents was the highest among three groups. The score was lower in older doctors. The score was low among those who thought doctors' socioeconomic status was not good. The longer the work time, the higher the job stress score was. Multiple regression analysis was conducted to control for the mutual influence of independent variables. In regression analysis, the score of residents was higher than practitioners. Work time and socioeconomic status perception had negative effects on job stress score. Conclusion : The average job stress score of the doctors was high. Age, work type, working conditions and perceptions of socioeconomic status were found to besignificantly related to job stress score. Although the job stress of doctors is somewhat inevitable due to the nature of the doctor's job, control of work time, development of coping tools and other intervention methods are needed to reduce job stress of doctors. Further studies are required to understand the characteristics of iob stress and reduce the job stress of doctors.

농촌지역 주민의 고혈압 치료순응도와 관련요인

이상원,천병렬,예민해,강윤식,김건엽,이영숙,박기수,손재희,오희숙,안문영,임부돌,감신,Lee, Sang-Won,Chun, Byung-Yeol,Yeh, Min-Hae,Kang, Yun-Sik,Kim, Keon-Yeop,Lee, Young-Sook,Park, Ki-Soo,Son, Jae-Hee,Oh, Hee-Sook,Ahn, Moon-Young,Lim, Pu-D 대한예방의학회 2000 예방의학회지 Vol.33 No.2

Objective : The purpose of this study was to examine the therapeutic compliance and its related factors in the rural hypertensives. Method : A questionnaire survey and blood pressure measurement were performed to 3,876 residents of a rural area, and 660 hypertensives were selected as subjects of study. The study employed a hypothetical model which was composed of constructs from the health belief model and KAP model. The analysis techniques employed included contingency table analysis and structural equation modeling. Result : The proportion of those who were compliant to the treatment of hypertension was 44.2% of subjects. As the result of structural equation modeling, when patients had more favorable attitude toward treatment, higher perceived benefit, or lower perceived barriers to treatment, the therapeutic compliance was significantly higher(T>2.0). When patients had more knowledge about hypertension, or higher perceived severity of hypertension, the attitude toward the treatment of hypertension was more favorable significantly(T>2.0). And when patients had the support for treatment from family or neighbor, the attitude toward treatment was more favorable(T>2.0). When patients had experience of health education, they had more knowledge, higher perceived susceptibility of complication, perceived severity for hypertension, and perceived benefit of treatment, compare to patients without health education(T>2.0). Conclusion : In consideration of above findings, in order to improve the therapeutic compliance in the rural hypertensives, it would be necessary to change attitude, perception, knowledge about hypertension and its treatment, by various methods such as effective health education and programs for maintaining the supportive environment for hypertension treatment.

농촌 지역 주민들의 고혈압 발생 위험요인 - 1년간 전향성 추적 조사 -

오희숙,감신,예민해,강윤식,김건엽,이영숙,박기수,손재희,이상원,안문영,천병렬,Oh, Hee-Sook,Kam, Sin,Yeh, Min-Hae,Kang, Yun-Sik,Kim, Keon-Yeop,Lee, Young-Sook,Park, Ki-Soo,Son, Jae-Hee,Lee, Sang-Won,Ahn, Moon-Young,Chun, Byung-Yeol 대한예방의학회 2000 예방의학회지 Vol.33 No.2

농촌지역 성인들에서 고혈압 발생과 관련이 있는 위험요인을 조사하기 위하여 1996년 경상북도 청송군의 20세이상 성인 3,573명을 대상으로 면담조사와 신체검사를 실시하였다. 연구대상 코호트는 고혈압이 없는 2,580명으로 하였는데, 1년 후 1,781명(69.0%)의 추적조사가 완료되었다. 조사한 독립변수들은 일반적 특성(연령, 성, 교육수준, 경제적상태, 결혼상태), 고혈압 가족력, 식이, 술, 담배, 커피, 스트레스, 그리고 여성에서 경구용 피임약, 폐경상태가 추가되었다. 그리고, 키, 몸무게, 허리둘레, 엉덩이둘레, 초기혈압, 혈청 총 콜레스테롤이었다. 다중 로지스틱 회귀분석 결과, 남자에서는 연령의 비교위험도가 1.50(95% 신뢰구간; 1.15-1.96), 고혈압 가족력이 2.11(95% 신뢰구간; 1.04-4.26), 허리-엉덩이 둘레비가 2.09(95% 신뢰구간; 1.15-3.79), 초기 수축기 혈압은 120mmHg 미만을 기준으로 하였을 때 130-139mmHg가 3.34(95% 신뢰구간; 1.47-7.60)로 경계형이상 고혈압의 발생과 유의한 관련이 있는 위험요인이었다. 여자에서는 연령의 비교 위험도가 1.06(95% 신뢰 구간; 1.03-1.09), 추적 기간동안 새로이 폐경을 경험한 경우는 3.32(95% 신뢰구간; 1.01-10.87), 초기 수축기 혈압은 120mmHg미만을 기준으로 하였을 때 120-129mmHg가 2.00(95% 신뢰구간; 1.02-3.90), 130-139mmHg가 2.64(95% 신뢰구간; 1.34-5.20), 그리고 초기 이완기 혈압은 80mmHg미만을 기준으로 하였을 때 85-89mmHg가 4.09(95% 신뢰구간; 1.86-8.96)로 유의한 관련이 있는 위험요인이었다. 결론적으로 경 계형 이상 고혈압 발생과 관련된 위험요인으로는 남자에서는 연령, 고혈압 가족력, 허리-엉덩이 둘레비 그리고 높은 정상 혈압이었다. 여자에서는 연령, 폐경이 새롭게 일어난 경우 그리고 높은 정상 혈압이었다. Objectives : This study was peformed to identify the risk factors related to the development of hypertension in a rural area. Method : Total of 3,573 subjects in Chung-Song County were interviewed and examined in 1996. The study cohort comprised 2,580 hypertension-free subjects aged above 20. One-year follow up was completed for 1,781 subjects(69.0%) in 1997. General characteristics(age, gender, education level, economic status, marital status), the family history of hypertension, diet, alcohol, smoking, coffee, stress, past history of oral contraceptive and menopausal status in female, height, weight, waist and hip circumference, baseline blood pressure, and serum total cholesterol were considered as risk factors. Results : Multivariate analysis using logistic regression model indicated that age(RH=1.50, 95% CI; 1.15-1.96), the family history of hypertension(RR=2.11, 95% CI; 1.04-4.26), waist-hip ratio(WHR) (RR=2.09, 95%, CI; 1.15-3.79), and baseline systolic blood pressure(130-139/<120mmHg)(RR=3.34, 95% CI; 1.47-7.60) were significant risk factors associated with the development of hypertension above the borderline level in male. In female, age(RR=1.06, 95% CI; 1.03-1.09), change in menopausal status$(no{\rightarrow}yes/no{\rightarrow}no)$ (RR=3.32, 95% CI; 1.01-10.87), baseline systolic blood pressure(120-129/<120mmHg: RR=2.00, 95% CI; 1.02-3.90)(130-139/<120mmHg: RR=2.64, 95% CI; 1.34-5.20) and baseline diastolic blood pressure(85-89/<80mmHg)(RR=4.09, 95% CI; 1.86-8.96) were identified as risk factors. Conclusions : Age and high normal blood pressure were significant risk factors for the development of hypertension above the borderline level. In addition, the family history of hypertension and WHR in men, and the change of menopausal status in women might be significant risk factors in Korea.

개원의사와 개원한의사의 전문직업성, 직무스트레스 및 직업만족도

김종연,강윤식,조용기,이상원,진대구,안순기,천병렬,예민해,감신,Kim, Jong-Yeon,Kang, Yune-Sik,Cho, Yong-Kee,Lee, Sang-Won,Jin, Dae-Gu,Ahn, Soon-Gi,Chun, Byung-Yeol,Yeh, Min-Hae,Kam, Sin 대한예방의학회 2004 예방의학회지 Vol.37 No.1

Objectives : This study was conducted in order to investigate the professionalism, job stress and job satisfaction in western and oriental doctors in Korea. Methods : The authors conducted a survey using a self-administered questionnaire, conducted between August and September, 2001. The study subjects were 457 western doctors and 161 oriental doctors practicing at local clinics in Daegu City. Results : For the professionalism scale, the score for belief in service to the public of western doctors was significantly higher than that of oriental doctors (p<0.05) whereas the scores for sense of calling to the field and feeling of autonomy were higher in oriental doctors (p<0.01). For the job stress scale, the scores for work factor, and clinical responsibility/decision factor of western doctors were significantly higher than those of oriental doctors (p<0.01). Of the western doctors, 59.7% expressed satisfaction with their job as a doctor, 69.5% responded that the role of a doctor was appropriate to their aptitude, and 61.8% answered that they wouldn't consider other kinds of job even if offered. In case of oriental doctors, these responses were 83.1%, 82.5%, and 82.3%, respectively. Conclusions : The job stress scores of western doctors were higher than those of oriental doctors, but the job satisfaction was lower. The reasons for the above results are not clear. Further studies are required to understand the characteristics of job stress and job satisfaction in doctors.