Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sohn, Sea-Hwan,Cho, Eun-Jung,Jang, In-Surk The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.4

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sea Hwan Sohn,Eun Jung Cho,In Surk Jang 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.4

The Chinese Hamster Ovarian cells CHOK1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHOK1 cells. The GTGbanding and fluorescence in situ hybridization on CHOK1 cells were performed to characterize the karyotype and the distribution of telomeric DNA. The present study revealed that the chromosome modal number of CHOK1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHOK1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHOK1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

重化學工業機械의 國産化方案에 關한 硏究 : 特히 窯業에 있어서의 燒成爐, 粉碎機, 排風機, 冷却機, 電氣集마器 自動枰量供給器 等의 製作을 目的으로

趙哲衡,朴碩喆,丁太權,宋鐵,桭達福,金基玉,朴煥奎,趙煥從,朴善鐘,金種一,李茂錫 朝鮮大學校 1977 綜合論文集 Vol.1977 No.-

This is to investigate the posibility of home manufacturing of heavy chemical industry machines, such as rotary kiln, crusher, blower, cooler, electrostatic precipitator and weighing feeder of cement plant. It is concluded that even though we can not make all of them (some of them are made now and some others are going to be made in the near future, some of them are made whole and some others are made partially), we can build or export the cement plant by importing the important machines which we can not make now and by substituting them with ours gradually.

야생동충하초로부터 우량균주선발(II)

방극소(Keuk-So Pang),홍경환(Kyung-Hwan Hong),조덕현(Duck-Hyun Cho) 한국자원식물학회 2001 한국자원식물학회지 Vol.14 No.2

2000년 5월 중순부터 10월까지 채집하였다. 이 중 2속 4종을 확인하였고 6균주를 분리하여 모두를 자실체 유도실험에 이용하였다. 그 결과 동충하초 [Corcyceps miliraris(CHO-7410)] 1균주, 눈꽃동충하초[isaria japoica (CHO-6713, CHO-7413)] 2균주를 상업적이나 의약품개발에 있어서의 시료등 각 산업에있어서 전반적으로 이용가치가 충분히 있다고 사료되어 우량균주로 선발하였다. Many Cordyceps spp. were collected for excellent strains selection at near Wanju-kun from May to October, 2000. They were identified. As the resulting, They were 2 genera and 4 species. 6 strains were got through 4 species culture. 6 strains are Cordyceps militaris f. albino, C, gracilaides, C. militaris and Isaria japoica. Among them, 4 species used for induction of artifitial fruiting body. 3 strains of them were selected for excellent strains. excellent strains are Cordyceps militaris and Isaria japoica.

상악동에 발생한 국균증의 치험2례

조재오,김영균,여환호,김수민,김수관 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Aspergillosis of the maxillary sinus was rare disease, but it was increasing tendency with overuse antibiotics, streoid hormones, and anticanncerous agents. The clinical features and X-ray findings are similar to chronic suppurative paranasal sinusitis. Aspergillosis of the maxillary siuses may occur as a chronic diseae in an otherwise healthy person. This infection is usually confined to a single paranasal sinus, the maxillary antrum, though it can involve the orbit and may initiate with findings of proptosis and decreased vision. The disease is characterized by a wide range of initial symptoms, and should be considered as a possible diagnosis in sinusitis refractory to antibiotics and antral lavage. The choice of treatment of this disease is radical surgery and adjunctive systemic antifungal therapy. We experienced a case of right maxillary sinus aspergillosis in a 34 years old male who has dull pain on Rt. zygoma area, and a case of left maxillary sinus aspergillosis in a 30 years old male who had been suffered from nasal stiffness and frontal headache for 3-4 years. We reported two cases with review of literature.

종합병원 입원환자의 알코올중독 유병율과 주치의의 인지도 조사

조석군,김경빈,장환일 大韓神經精神醫學會 1993 신경정신의학 Vol.32 No.6

The authors studied prevalence and physician's detection rate of alcoholism in 478 adult patients admitted at Kyung-Hee Medical Center from June to July in 1992. In this study, the authors used self-report questionnaire consisting of Korean version of DSM-Ⅲ-R, MAST, NAST(I)(Alcoholism Screening Test of Seoul National Mental Hospital(I)), and additional demographic questions. The results were as following : 1) Among 478 patients, prevalence of alcoholism was 26.8%(76 patients) in male and 1.6%(e patients) in female. 2) Among the patients who are identified as alcoholism by the authors, detection rates of alcoholism by the charge physicians were 24.2% in internal medicine and none in other departments. 3) Compared to the alcoholic patients who are not detected by the charge physicians, the alcoholic patients who are detected are more aged patients showing high scored MAST significantly and having liver diseases.

農機械共同利用組織의 擴大發展方案에 關한 硏究 : 機械化 營農團을 中心으로 With reference to the Mechonized Farming Unit

曺在六,鄭煥庸 全南大學校企業經營硏究所 1981 産業經濟硏究 Vol.8 No.1

Ⅰ. Introduction Last two decades witnessed a rapid social transformation featured by urbanization along with industrialization. Spatial concentration of people and activities particularly in urban area has been the major feature in the course of the development efforts. It gave rise to the problem of rural exodus of the young, which has led to the reduction of the quantity and the deterioration of the quality of the rural labour. Therefore, it has become a sine qua non to induce mechanization of farming activities not only to make up for shortage of labour but to increase the agricultural productivity. The government came to enact a law in 1978 to promote farming mechanization. Acoording to this law, local governments are given commission to help organize and operate the mechanized farming units when necessary. It is only two years since the operation of the mechanized farming units began, it may be premature to assess the effect. However, it is an urgent need for the government and the people concerned to provide an efficient policy direction in order to support the operation of those units. To meet this need, this study aims to identify the problems arising from their operations with a view to provision of strategies for improvment of the operations and management of the new system. Ⅱ. Type and Present Situation of the Mechanized Farming Units Mechanized farming units are generally in operation with the aims of the reduction of farming labour cost, joint share of the purchasing expenses, collective acquirement of operation and managing techniques, joint performant of its related works, and diversification of risks in the use of machines. Therefore, the types of mechenized cooperative farming organizations may depending upon the motivation, pattern of operations, and the objective or the subject of the research. Based upon the subect, we can classify most of the units into two different kinds; one is the mechanized farming unit of the cooperative type, and the other of the village type. The former may be said to be led by the government, and the latter by the individual farm household. In chonnam province, the mechanized farming units have different names which reflect similar ideas without any deliberate any deliberate attempt to distinguish between them. The terms such as comprehensive mechanized demonstration unit, mechanized farming center, saemaul farming unit, and saemaul quasi-farming unit refer to different size of the units in terms of the operation. As of 1981, there are seventy two units of farm machine work in the province; one unit of the cowprehensive farming machines for demonstration purpose, six mechanized farming centers, forty saemaul farming units, and twenty five saemaul quasi-farming units. Ⅲ. Problems and Issues identified. The problems and issues observed in the process of this study can be devided into two different kinds, one kind is related to the operation itself, and the other is related to the management of the organization. The problems arising from the operation are follows; 1. Difficulties related to the procedures for mortgage to secure the loan for the purchase of farming machines 2. Delayed supply of farming machines 3. Low rate of farmers' participation in the operation, because most units are in operation mainly by the youth club 4. Depository fee is calculated in general based on the customary or traditional levels. It has to be based upon the cost accounting. The problems arising from the management of the units are as follows: 1. Frequent machine troubles due to unskilled operation techniques of those who are handling farming machines 2. Insufficient delivery systems to secure better access to repair services. 3. Difficulties related to obtaining parts of the farming machines imported from overseas Ⅳ. Guidliness for the Promotion of Mechanized Farming Units It is desirable to foster in prority the mechanized farming unit of village type which will bring up the client participation in the utilization of the system where by problems in operation and management might be solved with ease. The units of the cooperative type should perform the function of demonstration effect. It is also desirable to trust the operation of the units to the control of Saemaul leaders. Saemaul leaders of adjacent villages may organize a commission, if necessary, to decide the ways to utilize the farming machines for the common interest. In terms of adequate size of the unit, the composition of the farming machines should be based upon the combination of working efficiency of the machinery. The working capacity of each farming unit in the province has been calculated as 43.2 hectares of transplanting and 10.8 hectares of harvesting, given the present condition that each farming unit has two transplanters, one combiner(harvester), two tractors, etc. Ⅴ. Conclusion Policy Measures for Mechanized Farming Units are recommended as following. 1. The Government should enforce its policy to speed up the land consolidation to provide the favorable foundation for the mechanization of farming activities. 2. It is most recommendable to integrate supporting agencies in existance into one. 3. Government supports should be extended toward village type farming units focussing on those activities as transplanting and harvesting. Full-utilization of the existing farming machinery owned by individual farm household will be complementary 4. It is necessary to introduce a sort of insurance system against the crop damage in order to give favorable incentives to the probable trusters of farming activities. If the landholders thought it a dangerous venture to trust farming activities to the mechanized farming unit, the process of farming mechanization would be delayed.

안와를 침범한 상악동 악성종양에서 안구 보존을 위한 내시경의 역할

조규섭,노환중 부산대학교 병원 암연구소 2008 부산대병원학술지 Vol.- No.24

Background and Objectives: The periorbita has been regarded as the crucial structure in decision of orbital exenteration in the patients with paranasal malignancies. The mass beyond the thickened periorbita on T2-weighted images was considered to be a positive finding of orbital invasion. The purpose of this study is to evaluate the oncological safety of endoscopic removal of infiltrated tumor onto the periorbita but not transgress into orbital fat. Patients and Methods: Five patients of advanced maxillary cancer, showing bony orbital wall destruction and infiltration onto the periorbita but not transgress into orbital fat, underwent partial or total maxillectomy. Preoperative Cl and MRI were performed in all cases and compared with intraoperative findings. The successful dissection between tumors and periorbita using bipolar nasal coagulation forceps and tumor forcep was performed under endoscopy. Results: The mean age was 54.4 (41-74) years. All tumors of five patients originated from maxillary sinus and extended to involve multiple different subsites according to AJCC (2002, 6^(th) ed.) Histopathology included four squamous cell carcinomas and one adenoid cystic carcinoma. Follow-up ranged from 30 to 121 months (mean 53.6 months). All cases showed no local recurrence on the periobita after endoscopic removal. One patient had local recurrence in the pterygopalatine fossa and the other had in the neck. Conclusion: Endoscopic removal of infiltrated tumor onto the periorbita using microdebrider, bipolar nasal coagulation forceps and tumor forceps can be oncologically safe technique in advanced maxillary cancer infiltrated onto the periorbita but not invaded into orbital fat.

악관절 내장증의 외과적 치험례

조세인,여환호,김영균 대한 두개하악장애학회 1994 대한두개하악장애학회지 Vol.6 No.2

Internal derangememt of TMJ is defined as an abnormal relationship of articular disk to the Mandibular condyle, fossa and articular eminence. Anterior disk displacement of the disk causes the common symptoms of reciprocal clicking and limitation of mouth opening. Most Patients with internal derangements can be managed by conservative teatments. But surgical treatment is indicated for those patients who have chronic internal derangement that has failed to respond to nonsurgical treatment. The objective of surgical treatment is to eliminate pain and to restore normal function. Meniscectomy is indicated for the internal derangement of disk with perforation and gross morphological changes, nonreactive to conservative treatments. After meniscectomy, variable materials have been used for disk reconstruction. We present a case of interanal derangement of TMJ that was sucessfully treated by meniscetomy and autogenous auricular cartilage graft for disk replacement.

진행암 환자에서 Cisplatin 병용화학요법 시 Ondansetron의 오심 구토 조절 효과

조문준,윤환중,전의건,길준영,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Ondansetron is a novel agent that selectively binds to the 5-hydroxytryptamine_3 receptor, and has been reported to have a prominent effect in the prevention of anti-neoplastic agent induced nausea and vomiting. Twenty solid tumor patients who were scheduled to receive cisplatin containing combination chemotherapy participated in a prospectively open-labeled study to evaluate the antiernetic efficacy and safety of ondansetron. The male to female ratio was 11 : 9 and median age was 49(16-70). The sites of primary neoplasms and number of patients were as following : head and neck 4, metastatic carcinoma of unknown primary site 3, stomach 3, osteosarcoma 2, ovary 2, esophagus 1, melanoma 1, penile 1, bladder 1, cervix 1, and extragonadal germ cell 1. Ondansetron was given as an 8mg loading dose IV before chemotherapy followed by 8mg IV every 8 hours until 24 hours after chemotherapy completion. Complete or major control(0 to 2 emetic episodes) of emesis was achieved in 17 of 20 patients(85%;complete 50%, major 35%) receiving ondansetron during the first 24hrs of chemotherapy. During the period of day 2 through clay 5 of chemotherapy, 14 of 20(75%) patients had complete or major control of emesis(complete 35%, major 35%). No severe side reactions were recorded in ondansetron treated patients, while mild to moderate headache was noted in 20% of patients. These results show that ondansetron is effective in the control of cisplatin induced nausea and emesis, and can be administered safely with minimal side effects.