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유방통을 호소하는 한국 여성에서 Gamma Linolenic acid의 임상적인 효과
정재헌,김권천,조현진,민영돈,김성환,김정용,장정환 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1
Background and Objectives : Recently, the incidence of breast cancer is gradually increasing in korea. Many patients visit the hospital with a fear of breast cancer, if she had the pain in her breast. But Breast pain is not a cardinal sign of breast cancer. Only 7% of patient in breast cancer has breast pain. Materials and Method : To know the clinical effects of gamma-linolenic acid(GLA), we analyzed the medical records of 55 cases with breast pain, who visited the outpatient clinic of Chosun university hospital, between July of 1999 to December of 1999. Of the 55cases of breast pain, we analyzed 26 cases of patient with breast pain who was treated with GLA for 2 month and we excluded patients of breast pain who had breast mass or were treated with other drug. Results : 2 Patients we are dropped out because nausea and vomiting were developed, 4 Patient with breast pain were treated with GLA for 1 month, and relieved completely from mastalgia and 8 patients with breast pain were treated with GLA for 2 months and breast pain was relieved completelys. 4 patient had treatment for 2 months, were relieved slightly. But 8 patients with breast pain didn't reveal the relief of symptom after the intake of GLA. Conclusion : We conclude that GLA could be considered a first line drug in patients with mastalgia.
Baculovirus를 이용한 Aujeszky's Disease Virus gⅢ 단백질 발현
송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-
The g Ⅲ gene located in U_L region of Yangsan strain, a field isolate of Aujeszky's disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The gⅢ gene consisting of 1,437 nucleotides showed 98% sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gⅢ of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed gⅢ was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-gⅢ monoclonal antibody. The partially purified gⅢ protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect to specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gⅢ protein antigen (98%) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false nagative reactions in gⅢ RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.
요천추부 신경근병증으로 진단된 환자에서 통증정도와 자기공명영상 및 근전도 검사와의 상관관계
최광석,강정훈,김권영 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1
Objective: The purpose of this study was to compare the extent of pain with the severity of MRI, needle EMG findings, so as to find out which diagnostic method is more meaningfully represents the extent of pain. Methods: We investigated fifty-three patients who were underwent both MRI and Electrodiagnostic evaluation. MRI findings are classified as four groups which are Disc bulging, protrusion, extrusion or hernia and sequestration. EMG findings are classified as three groups based on the Wilbom's severity classification. Visual analogue scale are described as numbers between 0 to 10 due to extent of pain. We have compared the extent of pain with seventy in MRI and needle EMG findings. Results: Correlations between extent of pain and the seventy in MRI findings shows no meaningful interrelations, while correlations between extent of pain and the severity of needle EMG findings shows meaningful interrelations, Conclusion: Needle EMG might considered as a clinical method to quantify the extent of pain in the patients with radiculopathy who suffers from low back pain and radiating pain to lower extremities.
( Jung Hun Song ),( Soung Jung Kim ),( Min Hee Lee ),( Ki Cheol Park ),( Jung Hwan Hwang ),( Gi Ryang Kweon ),( Young Suk Jo ),( Heung-kyu Ro ),( Minho Shong ) 대한갑상선학회 2008 International Journal of Thyroidology Vol.1 No.1
Background and Objectives: Unlike proliferation, the signaling pathways of translation mechanism by TSH are not well defined. Therefore, we investigate the role of TSH and methimazole (MMI), an antithyroid drug, in the translational control of protein synthesis in thyrocytes. Materials and Methods: Expression and phosphorylation of translation regulatory proteins were determined by Western blot analysis and eukaryotic translation initiation factor 4E (eIF4E) bound eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1) was measured by immunoblot analysis and 7-methyl-GTP-sepharose-binding assay. Protein synthesis was monitored by incorporation of [<sup>35</sup>S]methionine and AMP-activated protein kinase (AMPK) activity was measured by in vitro kinase assay. Results: TSH regulates phosphorylation of downstream effectors of phosphatidylinositol 3 kinase (PI3K) signaling, such as Akt, 4E-BP1, and ribosomal protein S6 (rpS6). TSH-dependent 4E-BP1 phosphorylation and its subsequent dissociation from eIF4E resulted in activation of cap-dependent translation initiation. TSH-mediated activation of cap-dependent protein biosynthesis occurred through TSH receptor- (TSHR-), PI3Kand mTOR-dependent signaling pathways. Methimazole increased AMPK activity in FRTL-5 cells. Activation of AMPK resulted in down-regulation of mTOR-dependent translation initiation mediated by phosphorylated 4E-BP1 and rpS6. Conclusion: TSH regulates cap-dependent translation initiation through PI3K- and mTOR-dependent pathways. MMI inhibits these effects of TSH through activation of AMPK and inhibition of mTOR.
( Sang Hun Jung ),( Yong Hyun Lee ),( Dae Young Jeong ),( Yoo Ra Jeong ),( Byoung Ok Jung ),( Jae Kweon Park ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.2
Chitosan oligosaccharides designated to COS were derived from the hydrolysis of high molecular weight chitosan (HMWC). The range of molecular weight of COS was estimated to be approximately 0.5 to 2.5 kDa after the fractionation performed through gel-filtration, followed by MALDI-TOF mass spectrometry analysis. It was confirmed through the results that significant separation or fractionation was not observed well. Also, both dialyzed COS using a 12~14 kDa molecular weight cut-off (MWCO) membrane and salting-out COS were also showed no significant difference in the molecular weight of COS by MALDI-TOF mass spectrometry analysis. These results suggested that it is difficult to separate the chitosan hydrolysates bigger than 1 kDa according to the size (6-glucosamine) due to their high affinity between molecules which may tightly be associated together by hydrophobic or van der Waals force. Therefore, further examination on chitosan hydrolysis and fractionation for the production of COS with various sizes i.e., 7 or 8 units of glucosamine in large-scale, should be carefully considered for various applications.