論中國的飮食文化與生態環境 : 중국의 음식문화와 생태환경

宋燭華 漢陽大學校 民族學硏究所 1997 民族과文化 Vol.6 No.-

The Design and Implementation of Collaboration Service Integration Platform Based on Context-Aware Role Based Access Model

Shu-Ping Lu,Kuei-Kai Shao,Yu-Nung Chao,Kuo-Shu Luo,Chi-Hua Chen 보안공학연구지원센터 2014 International Journal of Security and Its Applicat Vol.8 No.1

Knockdown of an ABC transporter leads to bright red eyes in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Shu Hua Liu,Ai YingWang,Bao Jun Yang,Ju Luo,Jian Tang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Compound eye color is an important biological character of insects,which is determined by the nature of eye pigments. Ommochrome is the solely source of eye color for some insects, while pteridines is also needed for the other insect species. However, little is known about the eye pigment composition for any planthopper. Scarlet is an ABC (ATP-binding cassette) transporter protein, which functions as the transmembrane transporter for ommochrome precursor. The failure of Scarlet function can cause bright red or white eyes in different species, which depending on the nature of eye pigments. Here, we identified a scarlet ortholog gene (Nlst) from the brown planthopper (BPH), Nilaparvata lugens, which is a destructive insect pest of rice. Nlst is the first characterized eye pigment transporter gene from Hemipteran. NlSt, the protein deduced from Nlst, has an open reading frame (ORF) of 629 amino acidswith the two sequence logos for eye pigment transporters. Expression profile revealed that Nlst expressed at all development stages and had the highest transcript level in head. Knockdown the Nlst transcript, the wild-type eye color partially changed to bright red, while not to white. Meantime, the ommochrome level in heads reduced to 73.4%. These results suggested that the eye coloration of BPH needs both ommochrome and pteridines pigments. Because nymphal RNAi with Nlst leading to a clearly distinguished phenotype from the control individuals, Nlst maybe a suitable genetic marker to exploit embryonic RNAi technique in this insect pest.

Employing rotating vaneless diffuser to enhance the performance of plenum fan

Hua-Shu Dou,Lin Wu,Yikun Wei,Yongning Chen,Wenbin Cao,Cunlie Ying 한국유체기계학회 2017 International journal of fluid machinery and syste Vol.10 No.1

Numerical simulation is carried out for flow characteristics in a plenum fan and the influence of the diameter ratio of the rotating vaneless diffuser on the performance of plenum fan is analyzed. The diameter ratio of the rotating vaneless diffuser employed is from 1.03 to 1.3. The research results show that the rotating vaneless diffuser is able to enhance the performance of plenum fan. It is found that there is significant improvement in static pressure and efficiency at the diameter ratio of 1.05 at high flow coefficients, while the optimal diameter ratio is 1.2 at rated and low flow coefficient.

The white gene in Nilaparvata lugens and its expression pattern under two different survival stresses

Shu Hua Liu,Bao Jun Yang,Ai YingWang,Ju Luo,Jian Tang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

The brown planthopper (BPH), Nilaparvata lugens (Stål), is a serious pest of rice. Chemical control and pestresistant rice breeding are always two major management strategies of BPH. In order to keep effective control, understanding the detoxification mechanism of this insect pest appears to be high important. In addition to be an eye pigment transporter, White protein is also important for its non-eye roles, such as a potential target for insecticides and Bt toxin. In this study, we characterized the full-length white orthologues gene in N. lugens (Nlwhite). NlWhite, the deduced protein of Nlwhite, has feature motifs of the eye pigment transporter. Knocking down Nlwhite transcript, the compound eye color partially changed to white. Nlwhite can be detected in all developmental stages and body parts, indicating that it is not a developmental or tissue-specific expression gene. The expression levels of Nlwhite were all significantly upregulated in both pymetrozine-selection and Mudgoselection BPH strains, which were selected for several generations. These results indicated that NlWhite participated in the detoxification not only for the exogenous insecticides but also for the endogenous toxic substance.

An instability criterion for viscoelastic flow past a confined cylinder

Hua-Shu Dou,Nhan Phan-Thien 한국유변학회 2008 Korea-Australia rheology journal Vol.20 No.1

It has been known that there is a viscoelastic instability in the channel flow past a cylinder at high Deborah (De) number. Some of our numerical simulations and a boundary layer analysis indicated that this instability is related to the shear flow in the gap between the cylinder and the channel walls in our previous work. The critical condition for instability initiation may be related to an inflection velocity profile generated by the normal stress near the cylinder surface. At high De, the elastic normal stress coupling with the streamline curvature is responsible for the shear instability, which has been recognized by the community. In this study, an instability criterion for the flow problem is proposed based on the analysis on the pressure gradient and some supporting numerical simulations. The critical De number for various model fluids is given. It increases with the geometrical aspect ratio h/R (half channel width/cylinder radius) and depends on a viscosity ratio β (polymer viscosity/total viscosity) of the model. A shear thinning first normal stress coefficient will delay the instability. An excellent agreement between the predicted critical Deborah number and reported experiments is obtained.

Comparison of Transforming Growth Factor-Beta1 and Lovastatin on Differentiating Mesenchymal Stem Cells toward Nucleus Pulposus-like Phenotype: An In Vitro Cell Culture Study

Shu-Hua Yang,Kai-Chiang Yang,Chih-Wei Chen,Ting-Chun Huang,Yuanhui Sun,Ming-Hsiao Hu 대한척추외과학회 2019 Asian Spine Journal Vol.13 No.5

Study Design: In vitro cell culture study. Purpose: This study aims to investigate the impact of transforming growth factor-beta1 (TGF-β1) and lovastatin on differentiating human mesenchymal stem cells (MSCs) toward nucleus pulposus (NP)-like phenotype. Overview of Literature: MSCs offer a cell source to the cell-based therapy for intervertebral disc degeneration. TGF-β1 is used to induce MSCs to differentiate into NP-like cells; however, an undesired expression of collagen type I has been reported. Statins reportedly stimulate expression of bone morphogenetic protein-2 (BMP-2) and promote the chondrogenic phenotype to NP cells. However, the effects of statins with or without TGF-β1 on the differentiation of MSCs into NP-like cells remain unclear. Methods: Human MSCs were treated with TGF-β1 alone, lovastatin alone, and simultaneous or sequential treatment with TGF-β1 and lovastatin. After the proposed stimulation, the total RNA was extracted to assess the expression profile of NP cells-specific genes. Hematoxylin–eosin staining was used for examining the microscopic morphology. Furthermore, we detected the syntheses of S-100 protein, aggrecan , and collagen type II in the extracellular matrix using immunohistochemical staining. Results: Simultaneous or sequential treatment of TGF-β1 and lovastatin could further augment the BMP-2 overexpression compared with lovastatin-alone treatment. However, the mRNA expression of aggrecan and collagen type II was not compatible with the expression level of BMP-2 . Immunohistochemical studies revealed compatible production of aggrecan, collagen type II , and S-100 protein in all three groups treated with lovastatin. Cells in groups treated with lovastatin were less populated than that in the group treated with TGF-β1 alone. Conclusions: This study demonstrates a promising role of lovastatin in inducing human MSCs into NP-like cells. However, further optimization of cell density before lovastatin treatment, treatment duration, and combination with TGF-β1 are warranted to attain better stimulatory effects.

Identification and Characterization of the Antifungal Substances of a Novel Streptomyces cavourensis NA4s

( Hua Qi Pan ),( Su Ya Yu ),( Chun Feng Song ),( Nan Wang ),( Hui Ming Hua ),( Jiang Chun Hu ),( Shu Jin Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soilborne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological, and phylogenetic analyses based on its 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and a broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soilborne fungal diseases of plants.

Gene Expression Profile of Helicobacter pylori in Response to Growth Temperature Variation

Yue-hua Han,Wen-zhong Liu,Yao-zhou Shi,Li-qiong Lu,Shu-dong Xiao,Qing-hua Zhang 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

A Helicobacter pylori whole-genome DNA microarray was constructed to study expression profiles of H. pylori in response to a sudden temperature transfer from 37°C to 20°C. The expression level of the genome at each of four time points (15, 30, 60, and 120 min) after temperature downshift was compared with that just before cold treatment. Globally, 10.2% (n=167) of the total predicted H. pylori genes (n=1636) represented on the microarray were significantly differentially expressed (p<0.05) over a 120 min period after shift to low temperature. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated by quantitative real-time PCR. Up-regulated genes mainly included genes involved in energy metabolism and substance metabolism, cellular processes, protein fate, ribosomal protein genes, and hypothetical protein genes, which indicate the compensational responses of H. pylori to temperature downshift. Those genes play important roles in adaption to temperature downshift of H. pylori. Down-regulation of DNA metabolism genes and cell envelope genes and cellular processes genes may reflect damaged functions under low temperature, which is unfavorable to bacterial infection and propagation. Overall, this time-course study provides new insights into the primary response of H. pylori to a sudden temperature downshift, which allow the bacteria to survive and adapt to the new host environment.

中國古代小說的所指與類型

나서화 ( Shu Hua Luo ) 고려대학교 중국학연구소 2008 中國學論叢 Vol.23 No.-

중국 고대소설은 변해서 정의 내려지지 않은 명시로, 주로 패시소설, 기문소설, 평회소설, 장회소설 네 가지 유형이 있다. 소설이란 명칭은 이 네 가지 전혀 다른 문체를 근대에 와서 총칭한 것이다. 고대 소설은 문사에서 비롯해서, 또한 문사를 뛰어넘어, 문사에 녹아들었다. 그것은 詩,文,史 등의 다양한 문체를 겸비하면서도, 이야기의 성격을 벗어나지 못하고 있다. 그것은 허구적 이기도 하고 사실적이기도 하며, 고문과 백화, 장편과 단편이 있을 수 있지만 산문체를 위주로 하고 변려체를 운용하기도 한다. 이러한 소설관은 고대 소설관의 집대성이자, 현대 소설관의 원류라 할 수 있다.