A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

1989년 10월 12일 충남 홍성에서 발생한 토네이도

최부섭(Boo-Sub Choi),이언구(Eon-Gu Lee),홍성길(Sung-Gil Hong) 한국기상학회 1990 Asia-Pacific Journal of Atmospheric Sciences Vol.26 No.1

체육수업에 대한 교과 흥미도가 학생들의 학업열의에 미치는 영향

이상섭(Lee, Sang-Sub),조홍식(Cho, Hong-Sik) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.1

The purpose of this study was to investigate the effects of subject interest in physical education classes on academic engagement among students. For that purpose, the investigator found the population in middle and high school students in Gyeonggi Province and sampled 420 students through convenience sampling. Collected data were put to multiple regression analysis with the PASW Version 18 .0 statistical program. The findings were as follows: First, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement(efficacy). Second, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement(commitment) . Third, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement (vitality). Finally, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement (absorption)

체육수업의 재미요인이 학생들의 심리적 유인성향에 미치는 영향

이종철(Lee Jong-Chul),조홍식(Cho Hong-Sik),박윤혁(Park Yoon-Hyuk),이상섭(Lee Sang-Sub) 한국체육과학회 2006 한국체육과학회지 Vol.15 No.3

The purpose of this study was to explore ways to at identifying what factors made students felt enjoyment during physical education class and how much those factors influence on psychological of inducement inclination. To this end, Cluster Random Sampling was used to set up the population with boys and girls middle school students in Kyunggi-do and Inchon-si area. The survey tool was questionnaire and Collected data hereof were analyzed by one way ANOVA analysis and Multiple Regression Analysis with SPSS WIN Ver 11.5. The aforementioned method and procedure led this research to the conclusion as follows. Firstly, out of sub-factors of physical education class enyoyment by middle schoolers' individual characteristics(gender, result), boy students showed more than girl students, in which there were statistically significant difference. Meanwhile, statistically significant difference was not found in free time, explanation and modeling. Secondly, out of sub-factors of psychological of inducement inclination by middle schoolers' individual characteristics(gender, result), boy students' response was higher in senses of confidence, enthusiasm, accomplishment and indulgence than girl students' in which all considers appeared. Thirdly, the effect enjoymant factors of physical education class had statistically significant on sub-factors of psychological of inducement inclination senses of confidence, enthusiasm, accomplishment and indulgence, free time that is a sub-factor of enjoymant which did not had statistically significant influenced on sense of accomplishment.

Characterization of OsJAC1 which is responding to different types of ionizing radiation

In jung Jung,Jung Eun Hwang,Sung Min Han,Hong-Il Choi,Soon-Jae Kwon,Jin-Baek Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Ionizing radiation affects gene expression from plant genomes. To monitor the genome-wide transcriptional changes induced by three types of ionizing radiation, we used the rice RNA sequencing to identify genes that are up- or down-regulated by gamma rays (GAs), proton (PRs) and ion beams (IBs). The Oryza sativa jacalin-like lectin domain containing proteins (OsJAC1) gene was highly induced by GAs, PRs and IBs. OsJAC1 was selected based on the expression patterns of a genome-wide dataset of RNA sequencing. Many jacalin-related lectin genes have been shown to be associated with disease resistance, biotic and abiotic stress signaling. Therefore, we studied its expression pattern in response to different abiotic stress and phytohormone treatments. The expression patterns of OsJAC1 under two different abiotic stress conditions (salt and heat stress) and phytohormones (salicylic acid and methyl jasmonate) were examined. The transcripts of OsJAC1 were significantly induced in response to abiotic stress conditions, including salt and heat treatments. In addition, it was induced in response to the salicylic acid and methyl jasmonate treatments, respectively. To investigate the sub-cellular localization of OsJAC1, the gene was expressed as a fusion protein tagged with GFP, in tobacco leaf epidermis and examined under confocal microscope. The OsJAC1 was clearly localized at the nucleus. These results provide critical insights into the molecular functions of the rice jacalin-like lectin domain containing proteins as receptors of external signals.

수술후 장폐쇄증의 임상적 연구

손기섭,신홍식 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

This Clinical report is based on a review of the record of 85 cases with postoperative intestinal obstruction those were managed at the Department of General Surgery, College of Medicine, Chungnam National University during 6 years from Jan. 1987 to Nov. 1992. The results of the study are obtained as follows; 1. Most frequent symptoms were abdominal pain, vomiting and abdominal fullness 2. Most frequent physical signs were abdominal distension, abdominal tenderness, rebound tenderness, abnormal peristalsis, and dehydration. 3. 75(88%) of 85 patients were admitted 48 hours after onset of symptoms. 4. The most frequent interval between previous operation and admission was between 7 month to 12 month. 5. The previous abdominal operation leading to intestinal obstruction revealed Miles operation, gastroduodenal operation, and small bowel operation in order. 6. Among 8 cases who have received operation, resection were done in 4 cases, adhesiolysis in 2 cases, Bypass in 1 case, and enterostomy in 1 case respectively. 7. Analysis on conservative management group L-tube group versus MA tube group ① No of cases 18 cases(21%) 67cases(79%) ② Hospital day 8 days 11days ③ Naso-gastric decompression day 3 days 6days 8. Hospital days were revealed within 1 week in 35%, and 1-2weeks in 48%. so within 2 weeks were over 83%. 9. Conclusion: The patients of intestinal obstruction were resolved without reoperation through 6 days naso-gastric decompression in 77 cases, about 91% of cases postoperative intestinal obstruction.

학교 교육의 위기 : 그 원인과 대책

정홍섭 신라대학교 교육과학연구소 2000 교육과학연구 Vol.- No.5

Daucus Carota L.의 關花促進에 關한 低溫處理 適期에 對한 硏究

金弘燮 조선대학교 기초과학연구소 1984 自然科學硏究 Vol.7 No.1

The influence of low temperature on Daucus Carota L. stimulating the convertion of sexual reproduction period over vegetative reproduction period, was verified by the research of Many scholars, and the low temperature limit and the time period to obtain the desired results will report the results of an experiment so as to determine the proper temperature results were reported by Miller and Yamamodo et al. This paper will report the results of an experiment so as to determine the proper temperature regulation period, by determining the sensitivity stimulation period during the growing period of plants. Since the results of this paper alone are inconclusive, further research will be carried out and reported in the future by this writer.

외과 감염 환자에서 레포스포렌®의 효과

손기섭,장일성,신홍식 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.1

Cinical effect of Refosporen® treatment was performed for the prophylatic and therapeutic use in various disease of general surgery in CNUH, the following results were obtained. 1) Refosporen® was used in 3 prophylactic cases, 3 cases were recovered from surgery without any postoperative surgical infections, or complication. 2) in 12 theraputic cases, 11 cases reveal excellent effect and 1 case reveals good effect. 3) The main advantages are the low rate of development in resistant organism against the Refosporen® and little side effects to the patients, and superior activity against Enterococci there were not included in the spectrum of usual cephalosporin.