홀스타인 수소에 있어서 Chromium Picolinate 의 첨가가 성장성적, 혈액성상 및 도체품질에 미치는 영향

홍중산,김명국,김윤학,한승은,이홍구,이현준,최윤재 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.4

본 시험은 성장단계별로 서로 다른 수준의 CrP를 첨가하여 성장성적, 혈액성상 및 도체품질에 미치는 영향을 규명하고자 실시하였다. 시험 Ⅰ에서 일당증체, 일당건물섭취량 및 사료효율에는 유의적인 차이가 없었다(p<0.05). 혈액성상에서 혈장 Glucose와 PUN의 농도에는 영향이 없었지만 혈중 인슐린의 농도는 0.05% CrP첨가구가 대조구의 2배되는 1.47ng/㎖ 인데 반하여 혈중 NEFA의 농도는 59.00 mEq/㎗ 로 유의적으로 낮게 나타났다(P<0.05). 이는 인슐린이 농도의 증가 및 인슐린의 활성의 증가로 인하여 지방합성대사가 활발히 진행된 결과로 사료된다. 이러한 결과를 도체분석결과가 뒷받침 해주고 있는데 비록 통계적인 유의차는 없지만 0.05% CrP 첨가구에서는 첫 60일간, 0.025% CrP 첨가구에서는 첫 120일간 대조구보다 우수한 성적을 나타냈다. 혈장 Glucose와 PUN의 농도에는 영향이 없었다. 통계적인 유의차는 없었지만 혈 중 인슐린의 농도는 0.025% CrP 첨가구는 대조구보다 8.88% 높은 1.35ng/㎖로 나타났고 0.05% CrP 첨가구는 오히려 1.13ng/㎖로 대조구보다 낮게 나타났다. NEFA의 농도는 시험 Ⅰ에서와 같은 차이는 없었지만 CrP 첨가수준이 증가할수록 감소하는 추세를 나타냈고 등지방두께는 일정한 변화추세를 보이지 않았다. We conducted two experiments to evaluate the effects of chromium picolinate(CrP) on growth performance, carcass characteristics and plasma components in Holstein bulls. In trial Ⅰ, eight finishing Holstein bulls(300±6.99㎏) were allocated to 2 treatments(control and 0.05% CrP) with 4 replication for 10-months. In results, growth performance was not affected by CrP addition. The plasma insulin concentration in 0.05% CrP group was about 2 times higher than the control group of Holstein bulls. The levels of plasma NEFA were significantly decreased to 59.00 mEq/㎗ with 0.05% CrP treatment(P<0.05), but the levels of plasma glucose and PUN were not altered by 0.05% CrP treatment. The grade of carcass was not different between control and 0.05% CrP group, but back fat thickness in 0.05%, CrP group was increased in 22.33% compared with control group. In trial 2, fifteen growing-finishing Holstein bulls(160±4.63㎏) were allocated to 3 treatments(control, 0.025% CrP and 0.05% CrP) with 5 replication for 14-months. During the overall experimental period, growth performance was not affected by CrP levels. The levels of hormone and metabolites were not affected by CrP supplementation. The carcass characteristics were not different between control and treatment. These results show that the CrP may have no effects for beef cattle production because of degradation of CrP conjugation in the rumen. However treatment of short term provide a possibility the effects of development for lipogenesis.

양질 조사료의 보충과 황토의 첨가수준이 한우의 성장성적, 육질 등급 및 경제성에 미치는 영향

홍중산,이홍구,이보균,김준식,최윤재,김윤학,김명국 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.1

본 연구는 6개월령의 한우 36두를 이용하여 양질 조사료의 보충(rice straw;RT, rice straw+cubed roughage;RCT)과 황토의 첨가수준(0%, 1%, 2% 황토)이 생산성, 육질 및 경제적 효과에 미치는 영향을 규명하기 위하여 실시하였다. 실험기간동안 볏짚에 양질 조사료의 보충 (cubed roughage, RCT)은 볏짚단독 처리(RT)에 비하여 일당증체, 사료효율 및 출하체중에서 각각 10%, 6.8%, 8.2%로서 유의적으로 증가되었으나(P<0.05), 황토의 첨가는 한우의 성장성적에 뚜렷한 효과는 없는 것으로 나타났고, 2%의 첨가수준은 오히려 감소되는 경향을 보였다. 육질 및 경제성 분석에서는 RCT구는 뚜렷한 개선효과를 보였지만, RT구에서는 황토 1%에서만 첨가 효과가 있는 것으로 나타났다. RCT구에서는 1%, 2% 모두가 0%에 비하여 보다 개선된 결과를 나타내었다. 이와 같은 결과들은 한우 비육에 있어서 황토의 첨가는 성장성적에는 뚜렷한 효과가 나타나지 않았지만 육질과 경제적 효과에서는 1%의 첨가는 개선효과가 있는 것으로 사료되며, 고급육 생산을 위한 황토의 첨가시에는 양질의 조사료 보충이 경제적 효과성을 더욱 향상시킬 것으로 사료된다. This study was conducted to estimate the effects of high quality cubed roughage and red clay (0%, 1%, 2%) on growth performance, meat quality and economical benefits. Thirty-six 6 month-old Korean native cattle, averaging 156㎏ of BW, were assigned to six groups in a 2 (rice straw : RT, rice straw+cubed roughage : RCT)× 3 (0, 1, 2% red clay). During experimental period, the high quality cubed roughage added into rice straw treatment (RCT) was superior to the only rice straw treatment (RT) on daily gain and feed efficiency and final weight 10%, 6.8%, 8.2% (P<0.05) respectively. But the addition of red clay did not affect and even 2% addition decreased those parameters. For meat quality and economical analysis, the addition of high quality cubed roughage made an outstanding result and for RT treatment, only 1% red clay indicated good effects while both 1% and 2% red clay were better than 0% for RCT treatment. From these results, it is assumed that the addition of red clay for fattening Korean native cattle has no effects on growth performance but 1% treatment has good results on improving meat quality and economical benefits. It also suggests that the combination high quality roughage and concentrated red clay is beneficial for producing high quality meat in with economical benefits.

사료첨가제로서 황토와 올리고당이 홀스타인 어린송아지의 성장 및 항병력에 미치는 영향

김준식,최윤재,이보균,김명국,홍중산,김윤학,이홍구 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.4

This experiment was conducted to study effects of loess and a form of oligosaccharide (glucosamine-D) as feed additives for 4 days to 2 month-old Holstein calves on the disease resistance and the growth rate. The total weight gains(WG) of test group given 0.04% glucosamine-D and 3% loess as additives were 43.6㎏ and 37.2㎏, respectively, which were 10.1㎏ and 3.6㎏ higher weight gains compared to control group(33.5㎏). The potential influence of loess and glucosamine-D treatment on the distribution of various leukocyte cell types in the serum of test group was tested using flow cytometic method. CD4 antigen positive cells(i.e., T helper cell) were highest in test group feeding 0.04% glucosamine-D, white class II MHC-positive cell type(antigen presenting cells such as macrophages and dendritic cells) was lowest in 6% loess-feeding group. These results suggest that 3% loess and glucosamin-D were more effective as feed additives for 4 days to 2 month-old Holstein calves to increase the disease resistance and the growth rate than those of any other treatments.

Influence of Silicon Spray on Storability of Truss Tomato in MA Storage

( M. Z. Islam ),( M. A. Mele ),( M. J. Jeong ),( I. S. Kim ),( S. K. Hong ),( I. L. Choi ),( J. P. Baek ),( H. M. Kang ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2014 강원 농업생명환경연구 Vol.26 No.3

This study was conducted to find out the performance of silicon foliar spray treatment on storability of truss tomatoes (Lycopersicon esculentum Mill. cv. Campari and Temptation) in MA (20,000 cc/m2 · day· atm O2 permeability film) storage. Tomatoes were grown by hydroponics at Gangwon province in Korea during the winter, harvested at light red maturity stage and packaged with MA condition for stored at 8℃ and to investigate the storability. The silicon sprayed as a whole plant that displayed the utmost visual quality had the oxygen 5.94-12.28%, carbon dioxide 6.72-7.94% and ethylene concentration around 0.43-1.67 μL/L of ‘Campari’ and the oxygen 3.82-10.84%, carbon dioxide 7.40-10.20% and ethylene concentration around 0.69-1.75 μL/L of ‘Temptation’. The range of fresh weight loss of ‘Campari’ was less than 0.22% and ‘Temptation’ was less than 0.20% during the storage period at 8℃. The higher visual quality maintained by silicon sprayed as a whole plant than other treatments of both cultivars on the MA storage period at 8℃. The silicon sprayed as a whole plant had a positive effect to reduce the fungal incidence of both cultivars during the MA storage period at 8℃. Moreover, the silicon sprayed as a whole plant showed more prominent firmness than other treatments for storage days of both cultivars. Therefore, the silicon foliar sprays as whole plant has the positive effect to advance storability, reduced fungal incidence, maintained vitamin C, soluble solids and retain firmness.

Ultraviolet Light (UV-C) Irradiation Reduced Postharvest Fungal Incidence of Cherry Tomato

( M. Z. Islam ),( M. A. Mele ),( I. S. Kim ),( S. K. Hong ),( J. P. Baek ),( H. M. Kang ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2015 강원 농업생명환경연구 Vol.27 No.2

This study was conducted to reduce the postharvest fungal incidence of cherry tomato (Lycopersicon esculentum Mill. cv. Unicorn) by ultraviolet light (UV-C) irradiation in MA storage. Hydroponic grown red maturity stage of cherry tomatoes was collected from Gangwon province. Tomato fruits were inoculated with Botrytis cinerea (grey mould). Afterwards, ultraviolet irradiation (254 nm, UV-C) of 7, 14, 21 and 28 kJ/m2 treated to tomato fruits. And treated UV-C tomatoes were packed with MA condition (20,000 cc/m2.day.atm O2 permeability film) to store at 5˚C for 20 days with 85% relative humidity. The 28 kJ treatment which showed the best visual quality maintained around 3.30-6.60% of carbon dioxide concentration and 16.08-17.50% oxygen and 0.26-1.11 μL/L ethylene during the storage. As storage periods progressed, the fresh weight loss was increased but did not exceed the maximum permissible of fresh weight loss. The 28 kJ performed the highest firmness among the treatments. The UV-C treatments demonstrated the less fungal incidence than control. The 28 kJ treatments exhibited the best visual quality, firmness, titratable acidity and less fungal incidence among the treatments. So, ultraviolet (UV-C) irradiation reduced postharvest fungal incidence of cherry tomato.

Taurine Prevents Hypertension and Increases Exercise Capacity in Rats With Fructose-Induced Hypertension

Rahman, M. M.,Park, H. M.,Kim, S. J.,Go, H. K.,Kim, G. B.,Hong, C. U.,Lee, Y. U.,Kim, S. Z.,Kim, J. S.,Kang, H. S. Nature Publishing Group 2011 American journal of hypertension Vol.24 No.5

삼차원 프린팅 성능 측정 및 평가 연구

장종명 (Z. M. Zhang ),정믿음(M. E. Jung),홍주표(J. P. Hong) 한국정밀공학회 2016 한국정밀공학회 학술발표대회 논문집 Vol.2016 No.5월

FDM방식 삼차원프린터의 기초성형 특성파악

장종명(Z. M. Zhang),정믿음(M. E. Jung),홍주표(J. P. Hong) 한국소성가공학회 2016 한국소성가공학회 학술대회 논문집 Vol.2016 No.4

Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

Won, M,Park, K A,Byun, H S,Sohn, K-C,Kim, Y-R,Jeon, J,Hong, J H,Park, J,Seok, J H,Kim, J M,Yoon, W-H,Jang, I-S,Shen, H M,Liu, Z G,Hur, G M Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.12

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-κB (NF-κB) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-κB function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-κB activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis.

슬러리 코팅법에 의한 스테인레스 스틸 표면에서의 알루미늄 확산막 제조 및 용융탄산염 내에서의 내식 특성 연구

남석우,황응림,아나톨리 마가뉵,홍명자,임태훈,오인환,홍성안,Nam S. W.,Hwang E. R.,Magtanyuk A. P.,Hong M. Z.,Lim T. H.,Oh I. -H.,Hong S. -A. 한국전기화학회 2000 한국전기화학회지 Vol.3 No.3

용융탄산염 연료전지의 분리판 재료로 사용되는 스테인레스 스틸은 고온 용융탄산염 분위기에서 부식이 심각하여 일반적으로 표면에 알루미늄 확산막을 코팅함으로써 내식성을 향상시켜 사용하고 있다. 본 연구에서는 기존 방법에 비해 보다 경제적인 슬러리 페인팅 및 열처리에 의한 알루미늄 확산막 형성 방법을 고안하여, 스테인레스 스틸 시편 표면에 알루미늄 확산막을 코팅하고, 산화 분위기의 용융탄산염에서 부식 실험을 수행하였다. $650\~800^{\circ}C$에서 제작된 알루미늄 확산막의 두께는 $25\~80{\mu}m$였으며, 열처리 온도가 높고 열처리 시간이 증가할 수록 알루미늄 확산막의 두께가 증가하였다. 부식 실험 결과 스테인레스 스틸 316L의 용융탄산염에 대한 내식성은 알루미늄 확산막을 표면에 형성시킴으로써 크게 향상되었음을 확인하였다. 또한 분극 실험 결과 슬러리 페인팅 및 열처리 방법에 의하여 알루미늄 확산막이 형성된 시편은 기존의 IVD 및 열처리 방법에 의해 알루미늄 확산막이 제작된 시편과 유사하게 안정한 부동태 피막을 형성함으로써 스테인레스 스틸 316L의 부식을 효과적으로 억제시킴을 알 수 있었다. A stainless steel separator for a molten carbonate fuel cell is usually coated with aluminum diffusive layer to protect its surface against corrosion by the molten carbonate at high temperatures. In this study, a relatively simple method was devised to form the aluminum diffusive layer on a stainless steel substrate. Slurry coating of aluminum on the substrate followed by heat treatment under reducing atmosphere at $650\~800^{\circ}C$ produced the aluminum diffusive layer of $25\~80{\mu}m$ thickness. The thickness of aluminum diffusive layer increased with increasing the temperature or duration of the heat-treatment. The corrosion resistance against molten carbonate under oxidizing atmosphere was significantly improved by aluminum diffusive layer formed by the sluny painting and heat treatment method. Moreover, the sample prepared in this study showed corrosion behavior similar to the sample with aluminum diffusive layer prepared by ion vapor deposition and heat treatment.