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김동우,( Ho Phoung ),이지현,( Suresh Thogiti ),이도경,김재홍 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
The effect of temperature variations on the performance of dyesensitized solar cells (DSSC), based on quasi-solid electrolyte, has been studied under 1 sun irradiance. The cell performance was characterized with J-V curves obtained at different cell operating temperatures and electrochemical impedance spectroscopy measurements before and after the temperature treatments. The measured temperature range was from ca. 25 °C to 75 °C at an interval of ca. 10 °C. The results show the higher temperature, the higher photo-current density of DSSCs. At lower temperatures, the short-circuit current (JSC) is limited by the conductivity of electrolyte, while at higher temperatures, the JSC increases due to more pronounced charge transport. Highest energy conversion efficiency of 7.93% was obtained at 55 °C, due to the gains in JSC and FF.
Angelina F. Osabutey,A-Young Kim,Phoung Nguyen,Kyu Ha Oh,Hye-Rim Han,Young Ho Koh 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3
The biochemical characteristics of pine wood nematode (PWN)-secretory antigen 571 (PWN-SA571) identified from the PWN secretome and PWN-infected pine tree extracts (PWNIPT) were investigated. PWN-SA571 has homologs in various nematodes in diverse families and a signal peptide at the amino-terminal end. Since it was not possible to express the full-length PWN-SA571 protein, two peptides showing high antigenicity were synthesized and used as antigens for generating monoclonal antibodies (Mabs). Mab-secreting fusion hybridoma cell lines were selected based on immunoreactivity to free peptide and BSA-conjugated peptide (BSA pep) antigens by enzyme-linked immunosorbent assay for establishing Mab-secreting hybridoma lines. Ascites containing PWN-SA571-specific Mabs showed stronger immunoreactivity to PWNIPT than to healthy pine tree extracts. In addition, PWN-SA571-specific Mabs could recognize less than 20 ng of BSA pep in lateral flow assays. Furthermore, purified biotin-conjugated PWN-SA571-specific Mab IgG or IgM were able to detect BSA pep (< 15 ng) and PWN extracts (< 600 ng). Taken together, these results demonstrate that PWN-SA571-specific Mabs could detect PWN or PWNIPT extracts by ELISA, LFA, or dot blot analysis.