All-trans Retinoic Acid-Enhanced Matrix Metalloproteinase 2 Secretion of Human Monocytic THP-1

VU THI HIEN Gachon University 2017 국내석사

Matrix metalloproteinase-2 (MMP-2) also known as gelatinase A, belongs to (MMPs) family and involves in breakdown of extracellular matrix (ECM). Previous studies showed that MMP-2 not only participates in processes of invasion, metastasis and angiogenesis and it also plays an important role in inflammation by cleavage of inflammatory cytokines and chemokines. In this study, we investigated the effects of all-trans retinoic acid (ATRA), an active metabolite of vitamin A, on MMP-2 expression of human monocyte THP-1. Cells treated with various concentrations (from 0.1 nM to 1 μM or 10 μM) or various time points (from 6 h to 72 h) showed a significant increase in MMP-2 mRNA expression. However, cell surface MMP-2 protein was decreased. To evaluate the level of secreted-MMP-2 proteins, culture supernatant was collected, concentrated and subjected to western blot analysis or enzyme-linked immune-sorbent assay (ELISA). Upon ATRA treatment, MMP-2 secretion was induced in both time-and concentration-dependent manners. In Western blot data, MMP-2 secretion from THP-1 cells started to increase after 6 h and reached maximum after 48 h treatment with 100 nM ATRA. Similarly, the data obtained from ELISA indicated the same tendency. Experiments with agonists or antagonists for retinoic acid receptor (RAR) or retinoid X receptor (RXR) revealed that ATRA-induced MMP-2 secretion depends on classical RARα-RXRα heterodimer pathway. There was an increase of intracellular calcium level by ATRA treatment and this increase was inhibited by the treatment with RAR or RXR antagonists or calcium channel blockers Verapamil. Our results revealed that ATRA induced-MMP-2 secretion of human monocytes is mediated through RAR/RXR signaling pathway and dependent on calcium. Additional studies are necessary to fully elucidate the function of secreted MMP-2 induced by ATRA. Keywords: All-trans retinoic acid, Matrix metalloproteinase-2, Secretion, RAR/RXR, THP-1.

링 마우스 : 손가락 움직임에 의한 컴퓨터 무선 입력 장치

Phan, Ke Hien 한국산업기술대학교 일반대학원 2018 국내석사

국문요약 링 마우스: 손가락 움직임에 의한 컴퓨터 무선 입력 장치 한국산업기술대학교 일반대학원 전자공학과: 판게히엔 지도교수: 정 두 희 이 논문에서는 링과 같이 손가락으로 감싸는 컴퓨터 용 무선 장치를 연구했습니다. Ring Mouse는 Bluetooth를 통해 컴퓨터에 무선으로 연결된 마우스 역할을하는 스마트 장치입니다. 손가락 움직임으로 컴퓨터의 마우스 커서를 제어 할 수 있습니다. 마이크로 컨트롤러 Cortex M4와 함께 nRF52832를 사용함으로써 우리는 전력 효율이 높고 비용 효율적인 회로를 만들었습니다. 모션 데이터는 MPU-9250 모듈을 사용하여 획득합니다. 장치의 기계적 케이스는 3D 인쇄 기술로 설계 및 제작되었습니다. ABSTRACT Ring Mouse: A Wireless Input Device for Computers by Finger Motions By Phan Ke Hien Advisor: Professor Jung Doo Hee Course for Electronics Engineering Graduate School Korea Polytechnic University In this thesis, we studied a wireless device for the computer which is carried on the finger like a ring. Ring Mouse is a smart device that acts as a mouse that is connected wirelessly to a computer via Bluetooth. One can control the mouse cursor on the computer with finger motions. By using nRF52832 with microcontroller Cortex M4, we made a circuit that is power-efficient and cost-effective. Motion data is acquired by using an MPU-9250 module. The mechanical case of the device is designed and made by 3D printing technologies.

역사·문화거리의 특성을 고려한 도시재생에 관한 연구 : 베트남 하노이 구시가지의 역사·문화거리를 중심으로

Hien, Huynh Thi Thu 성균관대학교 일반대학원 2022 국내석사

After the reform and opening in 1986, Vietnam's economic and industrial development led to the concentration of the population in cities. The development of the central city of the new city destroyed the historical and cultural streets and caused the population and economic downturn of the old city. This urban development centered on new towns has created new social problems, and therefore emphasized the importance of "urban regeneration," the top priority to be addressed in urban planning. Since the 1980s, Vietnam has been attempting to redevelop the existing city center through urban regeneration projects that preserve and use historical and cultural streets. However, most cultural street regeneration methods focus only on spatial preservation without considering specific cultural characteristics and situations. This method creates an imbalance in the modern urban flow and context and functionally does not meet the needs and interests of modern people. This study aims to investigate the cases of regeneration of historical and cultural streets in Vietnam, especially in the Old Quarter of Hanoi, and analyze the cases to lay the foundation for future regeneration and preservation of historical and cultural streets. Firstly, the preservation and maintenance of history and culture not only preserve and preserve only a part of designated buildings and historical sites, but also protect the entire environment of the historical culture. Secondly, through the detailed design vision within the street, you can feel the uniqueness of the area and capture the scenery of the historical and cultural streets of the area. Details such as signboard design, road surface with historical traces, characteristic scenery, and symbolic and memorable street furniture show cultural characteristics. Historical and cultural streets have made the road more unique and different. Thirdly, while preserving historical and cultural elements, it plans to repeat its architectural position of adjusting the internal space and strengthening the structure. revive historical solidarity but meet commercial needs Fourthly, the distance structure is optimized. A rich plan for the spatial structure is needed by creating a space that fits the behavior and psychology of consumers while maintaining the memories and emotions of the city. Fifthly, the government strengthened public participation while leading the project. More and more people are recognizing the value of historical and cultural streets by inducing the general public to watch and experience history and culture and introducing private organizations or holding events. It aims to form a comprehensive business model through experience activities that combine trade and culture, experience abundant consumers, extend people's shopping time, and preserve culture and develop the economy. This study investigated the actual case of the historical and cultural street regeneration method. It is hoped that the direction and research foundation for the regeneration of historical and cultural streets and urban development will be prepared. 1986년 개혁 개방 이후 베트남의 경제 및 산업 발전은 인구의 도시 집중 현상으로 이어졌다. 신도시의 중심 도시개발은 역사·문화거리를 파괴하고, 구시가지의 인구와 경제 침체를 초래했다. 이러한 신도시 중심의 도시개발은 새로운 사회문제를 야기시켰고, 따라서 도시계획에서 다루어야 할 최우선 과제인 "도시재생"의 중요성을 강조했다. 베트남은 1980년대부터 역사·문화거리 보존 및 이용방식의 도시재생사업을 통해 기존의 도심 재개발을 시도해왔다. 그러나 대부분의 문화적 거리 재생 방법은 특정한 문화적 특성과 상황을 고려하지 않고 공간 보존에만 초점을 맞추고 있다. 이 방식은 현대 도시 흐름과 맥락에 불균형을 조성하고 기능적으로 현대인의 필요와 관심을 충족시키지 못한다. 본 연구는 베트남, 특히 하노이의 구시가지에서 역사·문화거리 재생 사례를 조사하고, 그 사례들을 분석하여 향후 역사·문화거리 보존 및 재생 방안과 역사·문화거리 재생 방안을 위한 기반을 마련하기 위한 것이다. 첫째, 역사·문화의 보존과 유지는 지정된 건축물과 역사유적지의 일부만을 보존하고 보존할 뿐만 아니라 역사·문화권의 전체 환경을 보호한다. 둘째, 거리 내 상세설계 비전을 통해 그 지역의 독특함을 느낄 수 있고 그 지역의 역사·문화거리 풍경을 담았다. 간판 디자인, 역사적 흔적이 있는 노면, 특징적인 풍경, 상징적이고 기억적인 거리 가구 등 세세한 부분들이 문화적 특징을 보여준다. 역사·문화거리는 그 길을 더욱 독특하고 다르게 만들었다. 셋째, 역사·문화적 요소를 보존하면서 내부 공간을 조정하고 구조를 강화한다는 건축적 입장을 거듭할 계획이다. 역사적인 연대를 되살리지만, 상업적인 필요를 충족시키다. 넷째, 거리구조를 최적화한다. 도시의 기억과 감정을 그대로 유지하면서 소비자의 행동과 심리에 맞는 공간을 만들어 공간 구조에 대한 풍부한 계획이 필요하다. 다섯째, 정부는 사업을 주도하면서 대중 참여를 강화했다. 일반인의 역사·문화 관람과 체험을 유도하고 민간단체 소개나 행사 개최 등으로 역사·문화거리의 가치를 인식하는 사람이 늘고 있다. 무역과 문화를 결합한 체험 활동을 통해 종합 비즈니스 모델이 형성되고, 풍부한 소비자를 경험하고, 사람들의 쇼핑 시간을 연장하며, 문화 보존과 경제 발전을 목표로 하고 있다. 본 연구는 역사·문화거리 재생 방법의 실제 사례를 조사하였다. 역사·문화거리 재생과 도시개발의 방향 설정을 위한 기초자료로 활용되기를 기대한다.

Tribological characteristics of plasma sprayed zirconia coating and application to diesel engine simulation : Thuong-Hien Le

Le, Thuong-Hien 경북대학교 2006 국내박사

Antibody mimicking peptides from phage display: Potential applications in immuno separation

THAI BAO DIEU HIEN 경원대학교 일반대학원 2012 국내석사

Abstract Antibody mimicking peptides from phage display: Potential applications in immuno separation Thai Bao Dieu Hien Advised by Prof. Eun Kyu Lee Dept. Of Bionanotechnology Graduate School of Gachon University Phage display is a technology which can display the foreign peptides on the surface of the phages interacting with exterior molecules/materials. Such kind of peptides could demonstrate the same recognition and interaction capability compared to their native antibody molecules and yet possessing reduced sensitivity and improved stability characteristics. Phage display technique has been used to screen and identify the antibody mimicking peptides. In our study, the ‘biopanning’ was carried out by incubating a library of phage-displayed 12-mer peptides sequence (ca. 109 clones expressed as fusions on M13 pIII protein) on a solid surface coated with the target antigen (human IgG), washing away the unbound phages, and eluting the specifically bound phages. The eluted phages were taken through additional binding cycles to enrich the pool in favor of binding sequences. Our preliminary experiments resulted in 4 clones (F9, D1, G5, A10) showing specific binding to human IgG through enzyme linked immunosorbent assay (ELISA). Among them, F9 showed the highest affinity (Kd = 6.2 nM), only one order of magnitude lower than the native anti-human IgG (0.66 nM). Four 12-mer peptides having the specific amino acid sequences of the selected phages were chemically synthesized. Binding affinities of the synthesized peptides to hIgG were monitored using Surface Plasmon Resonance (SPR). The peptide D1 had a higher binding affinity against hIgG than the rest. This peptide was attached onto streptavidin –coated magnetic bead to determine the hIgG capturing ability and later to compare with immobilized anti- hIgG-bead. The result suggested that identified mimicking peptide from phage display was capable of capturing hIgG and showed the potential for immuno-magnetic separation.  

Standard of proof for establishing and recovering lost profits under the United Nations Convention on contracts for the international sale of goods, case studies and lessons on improving legal framework for Vietnam

Doan Thi Thu Hien TLBU Graduate School of Law 2019 국내석사

Fluorogenic probe for β-lactamase detection and DNA tetrahedron as a novel platform for targeted delivery

Hien, Thai Bao Dieu University of Science and Technology 2019 국내박사

β-lactamase is a bacterial enzyme that inactivates β-lactam antibiotic family by hydrolyzing the β-lactam core and confers the drug resistance to bacteria. Screening the β-lactamase producing organisms by detection β-lactamase activity is a useful strategy to promptly diagnosis and treatment of bacterial infection, preventing the infectious diseases from spreading to the public community. Fluorogenic probes are superior to well-established conventional methods in detecting β-lactamase activity owing to its high sensitivity, operational simplicity and cost-effectiveness. In part A of the thesis, two rational designs of fluorogenic probes were introduced to rapidly and sensitively detect β-lactamase activity. The first fluorogenic probe is a pH dependent self-quenching complex, composed of a quencher (poly-histidine) and a NTA- Atto488. The complex was formed by cross-linking the quencher and the fluorophore with a cobalt ion, which utilizes Histidine as ligands. Co (II) would be dissociated from the ligands under acidic or basic pH, triggering fluorescence recovery at both lowered and increased pH. This dual turn-on property of the fluorogenic probe could be applied for the assay of pH-changing enzymes. As the lowered pH is an indicator of the β-lactamase activity, I attempted to utilize the probe for β-lactamase assays. Indeed, the pH probe showed the enhanced fluorescence intensity when added to the enzymatic reaction mixture containing a β-lactamase and a β-lactam antibiotic. Further investigation was performed on lysate of β-lactamase-positive and β-lactamase-negative strains derived from infectious patients. While no obvious fluorescence signal could be observed in 20 β-lactamase negative strains, 21 out of 24 (87%) β-lactamase-positive strains showed clearly distinguished fluorescence signals. In addition to the pH- probe-based detection of beta-lactamase activity, a fluorogenic probe targeting hydrolysis of β-lactam ring of antibiotics was developed. The probe was designed to embed a β-lactam drug core in its structure. The fluorogenic probe was constituted by the conjugation of an alkylated pyridinium BODIPY and a cephalosporin-based structure. While the fluorescence of the alkylated pyridinium BODIPY was quite low, that of the pyridyl BODIPY released from the cephalosporin upon β-lactamase hydrolysis was relatively high. The proportional increase of fluorescence intensity to the concentration of enzyme demonstrated the probe sensing property for the activity of the enzyme. The probe was able to assay the enzyme and to determine kinetic parameters. Moreover, the probe was capable of detecting resistance strains to Ceftazidime- (CAZ-) and Cefotaxime- (CTX-) resistance strains with 85% sensitivity. In particular, the probe could identify the CAZ-resistance in bacteria that was not detectable using conventional pH-sensing probe, indicating the improved practical utility of the probe. In part B, the application of DNA tetrahedron was investigated as a drug delivery platform. DNA tetrahedron is a well-documented DNA nanostructure which possesses outstanding features such as the precisely defined structure, the relatively high stability in serum and the ease of fabrication. DNA tetrahedron is particularly useful carrier for delivery of nucleic acid cages into cells, as the cargo loaded DNA tetrahedron can be easily prepared in a seamless manner. Utilizing this advantage, I employed DNA tetrahedron as a carrier for intracellular delivery of DNAzyme and kidney delivery siRNA. DNAzyme is a synthetic oligonucleotide that can cleave the RNA substrate to exert the gene-silencing effect. However, the outcome of DNAzyme for therapeutic application is quite poor owing to its instability and low uptake efficiency. To overcome this drawback of DNAzyme I embedded a DNAzyme sequence into DNA tetrahedron, preparing tetrahedral DNAzyme (TDzs). TDZs showed the enhanced serum stability and higher cellular uptake efficiency compared to those of single stranded DNAzyme (ssDzs). The enhanced cellular uptake of TDZs significantly improved intracellular gene-silencing activity to down-regulate the target mRNA level. In addition to DNAzyme, the last chapter discussed the application of DNA tetrahedron for successful delivery of siRNA to kidney and protective effect of siRNA in acute kidney injury (AKI) in animal models. When four different backbone modified DNA tetrahedron were screened for kidney distribution, L-DNA tetrahedron (L-Td) was found to have the highest kidney accumulation level. To examine the siRNA delivery using L-Td as a carrier, p53-siRNA was conjugated with L-Td. The delivery capability of L-Td was evaluated by p53 knock-down efficiency in AKI mice model which was compared with naked siRNA. Since p53 plays a pivotal role in apoptosis regulation via caspase signaling pathway. The apoptosis level was also verified by Western blotting of caspase 3 expression and Annexin V-based assay. The histology kidney tissue was verified by H&E staining. The results revealed the lowered expression of caspase 3 and the partial recovery of damaged renal glomerulus and proximal tubules. Taken together, the acute kidney injury can be alleviated with the rational designed Td-p53, indicating that L-Td is an excellent carrier for kidney-targeted siRNA delivery.

Semi-supervised Learning with Local and Global Consistency on Bi-relational Graph for Image Annotation

Hien Duy Pham Pohang University of Science and Technology 2013 국내석사

We present a semi-supervised learning algorithm based on local and global consistency, working on a bi-relational graph of images and labels. By incorporating two types of dierent entities in a single graph, we exploit the label propagation to measure the relevance score between a specic label and unannotated images. The principle of the label propagation process is similar to many other semi-supervised learning methods, in which each node receives the information from its nearby points, and also retains its initial information. However, in our model, the neighbor concept is extended between dierent types of entities. As a results, the label correlation is captured, increasing the accuracy of image annotation. Moreover, in the propagation process, nodes from the same group are not treated equally, each has dierent relative reliability for the propagation process. We perform our method on two benchmark multi-label image data sets and gain encouraging experimental results compared to the existing work.

Synthesis and application of coordination-driven molecular assemblies and polymeric frameworks

Hien, Duy Mai 한림대학교 대학원 2017 국내박사

This thesis describes the fabrication of coordination-driven molecular assemblies and polymeric framework and their applications in gas adsorption and urea sensing. First, the fabrication of coordination-driven discrete molecular cage featuring a high level of structural hierarchy through a multiple-assembly process is demonstrated. The molecular cage shows much higher CO2 uptake property and selectivity compared with the separate supramolecular module tbu-Co-TSH and other molecular platforms. Second, nickel oxide nanostructures which are thermally transformed from nickel-based coordination polymer particles are fabricated and subsequently employed for the preparation of efficient urease-based biosensor for urea detection. Third, a facile and scalable synthetic strategy for unique gold multipod nanoparticle (GMN) core – zeolitic-imidazolate framework (ZIF-67) shell nanohybrids (GMN@ZIF-67) is reported.

스마트 그리드를 위한 최적 P2P 에너지 거래 시스템

Hien, Doan Thanh 순천향대학교 대학원 2023 국내박사