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Sekijima Hidehisa,Oshima Toru,Ueji Yuno,Kuno Naoko,Kondo Yukino,Nomura Saera,Asakura Tomomi,Sakai-Sugino Kae,Kawano Mitsuo,Komada Hiroshi,Kotani Hirokazu 한국독성학회 2023 Toxicological Research Vol.39 No.3
Benzalkonium chloride (BAC) intoxication causes fatal lung injuries, such as acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the pathogenesis of ALI/ARDS induced by BAC ingestion is poorly understood. This study aimed to clarify the mechanism of lung toxicity after BAC ingestion in a mouse model. BAC was orally administered to C57BL/6 mice at doses of 100, 250, and 1250 mg/kg. After administration, BAC concentrations in the blood and lungs were evaluated via liquid chromatography with tandem mass spectrometry. Lung tissue injury was evaluated via histological and protein analyses. Blood and lung BAC concentration levels after oral administration increased in a dose-dependent manner, with the concentrations directly proportional to the dose administered. The severity of lung injury worsened over time after the oral administration of 1250 mg/kg BAC. An increase in the terminal transferase dUTP nick end labeling-positive cells and cleaved caspase-3 levels was observed in the lungs after 1250 mg/kg BAC administration. In addition, increased cleaved caspase-9 levels and mitochondrial cytochrome c release into the cytosol were observed. These results suggest that lung tissue injury with excessive apoptosis contributes to BAC-induced ALI development and exacerbation. Our findings provide useful information for developing an effective treatment for ALI/ARDS induced by BAC ingestion.
( Satoshi Takeuchi ),( Hidehisa Saeki ),( Shoji Tokunaga ),( Makoto Sugaya ),( Hanako Ohmatsu ),( Yuichiro Tsunemi ),( Hideshi Torii ),( Koichiro Nakamura ),( Tamihiro Kawakami ),( Yoshinao Soma ),( E 대한피부과학회 2012 Annals of Dermatology Vol.24 No.2
Background: Pruritis caused by atopic dermatitis (AD) is not always well controlled by topical corticosteroid therapy, but use of tacrolimus often helps to soothe such intractable pruritis in clinical settings. Objective: To determine the anti-pruritic efficacy of topical tacrolimus in treating AD in induction and maintenance therapy. Methods: Prior to the study, patients were randomly allocated into two groups, induction therapy followed by tacrolimus monotherapy maintenance, and induction therapy followed by emollient- only maintenance. In the induction therapy, the patients were allowed to use topical tacrolimus and emollients in addition to a low dose (<10 g/week) of topical steroids. Patients showing relief from pruritis were allowed to proceed to maintenance therapy. Recurrence of pruritis in maintenance therapy was examined as a major endpoint. Results: Two-thirds of patients (44/68; 64.7%) showed relief from pruritis after induction therapy. Pruritis recurred in 23.8% (5/21) of the tacrolimus monotherapy group and in 100% (21/21) of the emollient group during maintenance period, a difference that was statistically significant. Conclusion: Use of topical tacrolimus is effective in controlling pruritis of AD compared to emollient. (Ann Dermatol 24(2) 144∼150, 2012)
Development of a WLAN Based Monitoring System for Group Activity Measurement in Real-Time
Hiroshi Tsunoda,Hidehisa Nakayama,Kohei Ohta,Akihiro Suzuki,Hiroki Nishiyama,Ryoichi Nagatomi,Kazuo Hashimoto,Yuji Waizumi,Glenn Mansfield Keeni,Yoshiaki Nemoto 한국통신학회 2011 Journal of communications and networks Vol.13 No.2
In recent years, there has been a rise in epidemiological evidence suggesting the health benefits of a physically active lifestyle. However, it is not always easy for individuals to personally recognize the optimal conditions for exercise and physical activity. Wearable acceleration-based pedometers have become widely used in estimating the amount of physical activity, and to a limited extent,providing information regarding exercise intensity, but they have never been used to assess adaptation to exercise. In order to realize simultaneous activity monitoring for multiple users exercising outdoors, we developed a prototype wireless local area network (WLAN) based system. In our system, a WLAN is deployed outside, and a user wearing a smart phone and monitoring device exercises freely within the coverage area of the wireless network. By doing so, the developed system is able to monitor the activity of each user andmeasures various parameters including those related to exercise adaptation. In a demonstration experiment, the developed system was evaluated and used to monitor users enjoying a Nordic walk, after which users were immediately able to receive their exercise report. In this paper, we discuss the requirements and issues in developing an activity monitoring system and report the findings we obtained through the demonstration experiment.
Mechanism of Macrophage-Derived Chemokine/CCL22 Production by HaCaT Keratinocytes
( Chizuko Yano ),( Hidehisa Saeki ),( Mayumi Komine ),( Shinji Kagami ),( Yuichiro Tsunemi ),( Mamitaro Ohtsuki ),( Hidemi Nakagawa ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.2
Background: CC chemokine ligand 17 (CCL17) and CCL22 are the functional ligands for CCR4. We previously reported that inhibitors of nuclear factor-kappa B and p38 mitogen- activated protein kinase (p38 MAPK), but not of extracellular signal-related kinase (ERK), inhibited tumor necrosis factor (TNF)-α- and interferon (IFN)-γ-induced production of CCL17 by the human keratinocyte cell line, HaCaT. Further, an inhibitor of epidermal growth factor receptor (EGFR) enhanced the CCL17 production by these keratinocytes. Objective: To identify the mechanism underlying CCL22 production by HaCaT cells. Methods: We investigated the signal transduction pathways by which TNF-α and IFN-γ stimulate HaCaT cells to produce CCL22 by adding various inhibitors. Results: TNF-α- and IFN-γ- induced CCL22 production was inhibited by PD98059, PD153035, Bay 11-7085, SB202190, c-Jun N-terminal kinase (JNK) inhibitor II, and Janus kinase (JAK) inhibitor 1. Conclusion: Our results indicate that CCL22 production in HaCaT cells is dependent on ERK, EGFR, p38 MAPK, JNK, and JAK and is mediated by different signal pathways from those regulating CCL17 production. Altogether, our previous and present results suggest that EGFR activation represses CCL17 but enhances CCL22 production by these cells.(Ann Dermatol 27(2) 152∼156, 2015)
Mechanism of Macrophage-Derived Chemokine/CCL22 Production by HaCaT Keratinocytes
( Chizuko Yano ),( Hidehisa Saeki ),( Mayumi Komine ),( Shinji Kagami ),( Yuichiro Tsunemi ),( Mamitaro Ohtsuki ),( Hidemi Nakagawa ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3
Background: CC chemokine ligand 17 (CCL17) and CCL22 are the functional ligands for CCR4. We previously reported that inhibitors of nuclear factor-kappa B and p38 mitogen- activated protein kinase (p38 MAPK), but not of extracellular signal-related kinase (ERK), inhibited tumor necrosis factor (TNF)-α- and interferon (IFN)-γ-induced production of CCL17 by the human keratinocyte cell line, HaCaT. Further, an inhibitor of epidermal growth factor receptor (EGFR) enhanced the CCL17 production by these keratinocytes. Objective: To identify the mechanism underlying CCL22 production by HaCaT cells. Methods: We investigated the signal transduction pathways by which TNF-α and IFN-γ stimulate HaCaT cells to produce CCL22 by adding various inhibitors. Results: TNF-α- and IFN-γ- induced CCL22 production was inhibited by PD98059, PD153035, Bay 11-7085, SB202190, c-Jun N-terminal kinase (JNK) inhibitor II, and Janus kinase (JAK) inhibitor 1. Conclusion: Our results indicate that CCL22 production in HaCaT cells is dependent on ERK, EGFR, p38 MAPK, JNK, and JAK and is mediated by different signal pathways from those regulating CCL17 production. Altogether, our previous and present results suggest that EGFR activation represses CCL17 but enhances CCL22 production by these cells. (Ann Dermatol 27(2) 152∼156, 2015)
Development of a WLAN Based Monitoring System for Group Activity Measurement in Real-Time
Tsunoda, Hiroshi,Nakayama, Hidehisa,Ohta, Kohei,Suzuki, Akihiro,Nishiyama, Hiroki,Nagatomi, Ryoichi,Hashimoto, Kazuo,Waizumi, Yuji,Keeni, Glenn Mansfield,Nemoto, Yoshiaki The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.2
In recent years, there has been a rise in epidemiological evidence suggesting the health benefits of a physically active lifestyle. However, it is not always easy for individuals to personally recognize the optimal conditions for exercise and physical activity. Wearable acceleration-based pedometers have become widely used in estimating the amount of physical activity, and to a limited extent, providing information regarding exercise intensity, but they have never been used to assess adaptation to exercise. In order to realize simultaneous activity monitoring for multiple users exercising outdoors, we developed a prototype wireless local area network (WLAN) based system. In our system, a WLAN is deployed outside, and a user wearing a smart phone and monitoring device exercises freely within the coverage area of the wireless network. By doing so, the developed system is able to monitor the activity of each user andmeasures various parameters including those related to exercise adaptation. In a demonstration experiment, the developed system was evaluated and used to monitor users enjoying a Nordic walk, after which users were immediately able to receive their exercise report. In this paper, we discuss the requirements and issues in developing an activity monitoring system and report the findings we obtained through the demonstration experiment.