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Effects of probiotics on intestinal health in high-salt diet (HSD) treated mice
Qiuxia Dong,Tingting Liang,Xuemei Cheng,Henghong Zhong,Zixian Wang,Wanying Li,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To observe the effect of probiotics on intestinal health in a model of high-salt diet (HSD, 8%) treated mice for 49 days. Cinical (body weight, colon length and colon weight) and historical changes were evaluated. In addition, colon levels of interleukin-1β, IL-6, IL-18, IL-17A, tumor necrosis factor (TNF)-α were detected by enzyme-linked immunosorbent assay. Relative to the HSD model group, the mice in the probiotics treatment group increased in body weight, colon length, and the ratio of colon weight to length was significantly lower (P<0.05). The DAI index of mice in the intervention group was lower than that in the HSD model group (P<0.05). Histological observation also found that the intervention of probiotics is able to reduce the infiltration of inflammatory cells in colon tissue induced by HSD. The results of Masson staining suggest that the intervention of probiotics also reduces the occurrence of intestinal fibrosis in mice with colitis. Our results suggested that probiotics with an activity to improve intestinal health and reduced inflammation in HSD treated mice.
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Jin-Ju Kim,이지선,Bo-Hyun Moon,이미옥,Seun-Hyun Song,Henghong Li,Albert J Fornace,차혁진 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.8
The optimization of in vitro culture conditions for embryonic stem cells (ESCs) is a matter of critical importance; a prompt supply of a sufficient population of cells that retain their pluripotency capabilities must be secured in order to make possible future cell therapies. Despite a number of reports asserting that a variety of cytokines, signaling ligands, and small molecules can help in maintaining the pluripotency of ESCs, mammalian feeder cells continue to be broadly accepted as the method of choice for ESC cultures. This appears to be because mammalian feeder cells seem to produce some as-yet-unidentified factor that makes them very effective as feeder cells. In this study, we investigated wild-type p53 inducible phosphatase (Wip1), the knockdown of which increases Wnt inhibitory factor-1 expression, in its feeder functions toward mouse embryonic stem cells, lowering the effect of Wnt, one of key signaling in maintaining stemness of ESCs. For this purpose, Wip1 was stably expressed in mouse embryonic fibroblast cell line (STO) using retro-viral gene delivery system and then the function as a feeder cell was monitored either with or without leukemia inhibitory factor (LIF) in culture medium. We demonstrated that mouse embryonic stem cells grown with Wip1expressing STO showed higher alkaline phosphatase activity and sustained Oct-4 expression level even under LIF deprivation condition compared to both control and Wip1 phosphatase activity dead mutant expressing STO. These results imply that Wip1 phosphatase activity in feeder cells is important to retain pluripotency of mouse embryonic stem cells under LIF deprivation conditions. These results indicate that genetically engineered feeder cells such as Wip1 expressing cell lines, are alternative strategy for the optimization of maintenance and expansion of mouse embryonic stem cells.
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