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RISS 인기검색어
- 검색키워드
- 저자 : Hehe Ren (검색결과 2 건)
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Spatial correlation-based WRF observation-nudging approach in simulating regional wind field
Hehe Ren,Shujin Laima,Wen-Li Chen,Anxin Guo,Hui Li 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.28 No.2
Accurately simulating the wind field of large-scale region, for instant urban areas, the locations of large span bridges, wind farms and so on, is very difficult, due to the complicated terrains or land surfaces. Currently, the regional wind field can be simulated through the combination of observation data and numerical model using observation-nudging in the Weather Research and Forecasting model (WRF). However, the main drawback of original observation-nudging method in WRF is the effects of observation on the surrounding field is fully mathematical express in terms of temporal and spatial, and it ignores the effects of terrain, wind direction and atmospheric circulation, while these are physically unreasonable for the turbulence. For these reasons, a spatial correlation-based observation-nudging method, which can take account the influence of complicated terrain, is proposed in the paper. The validation and comparation results show that proposed method can obtain more reasonable and accurate result than original observation-nudging method. Finally, the discussion of wind field along bridge span obtained from the simulation with spatial correlation-based observation-nudging method was carried out.
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Optimized study of the in vitro 3D culture of preantral follicles in mice
Hehe Ren 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.1
Background: In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E2) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. Conclusions: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.
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