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Heeyong Park,이성구,Tai Kyoung Kim,한세종,임정한 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6
An organic solvent for extracting prodigiosin from culture broth was selected and a test to determine the long-term stability of prodigiosin was performed to develop prodigiosin as a biological control agent against Chattonella antiqua, a harmful alga that can cause red tides. Prodigiosin was extracted using nine solvents, and the extracts were analyzed by liquid chromatography-mass spectroscopy. Acetone was selected as the best organic solvent because of its high extraction efficiency and less processing time. Stability tests for prodigiosin were performed at various temperatures, and algicidal activity against C. antiqua was also tested. Ultimately, > 98% stability was sustained after 30 days at 4℃, whereas < 30% stability was maintained after 30 days at 37℃. Although prodigiosin was kept for 30 days in an optimum organic solvent, its stability was safely maintained and algicidal activity was sustained at 4℃. These results indicate that acetone is a very useful extraction and storage solvent for prodigiosin. An organic solvent for extracting prodigiosin from culture broth was selected and a test to determine the long-term stability of prodigiosin was performed to develop prodigiosin as a biological control agent against Chattonella antiqua, a harmful alga that can cause red tides. Prodigiosin was extracted using nine solvents, and the extracts were analyzed by liquid chromatography-mass spectroscopy. Acetone was selected as the best organic solvent because of its high extraction efficiency and less processing time. Stability tests for prodigiosin were performed at various temperatures, and algicidal activity against C. antiqua was also tested. Ultimately, > 98% stability was sustained after 30 days at 4℃, whereas < 30% stability was maintained after 30 days at 37℃. Although prodigiosin was kept for 30 days in an optimum organic solvent, its stability was safely maintained and algicidal activity was sustained at 4℃. These results indicate that acetone is a very useful extraction and storage solvent for prodigiosin.
β-Cyclodextrin을 이용한 해수에서의 Prodigiosin의 안정성과 용해도 향상
박희용(Heeyong Park),김태경(Tai Kyoung Kim),한세종(Se Jong Han),임정한(Joung Han Yim) 한국생물공학회 2012 KSBB Journal Vol.27 No.2
This research was to examine the effects of various cyclodextrins on the solubility and stability of prodigiosin in seawater. Among them, β-cyclodextrin was found to have the best efficiency and formation of the inclusion complex was saturated when prodigiosin and β-cyclodextrin were mixed in a ratio of 1:8 and shaken at 25℃ and pH 8.0 for 6 h. The maximum algicidal activity against Chattonella antiqua using the inclusion complex stored at 4℃ for 5 weeks of culture was obtained, 52.28 ± 3.41%, which was about 5.0 fold higher than that of control. Our results suggest that inclusion complexes of prodigiosin and β-cyclodextrin could serve as effective algicidal agents.
유비쿼터스 환경에서 여행정보 서비스를 위한 상황정보 필터링 기법
박희용(Heeyong Park),허홍수(Hongsoo Hur),김정석(Jungsuk Kim),서경원(Kyungwon Seo),이무훈(Moohun Lee),조성훈(Sunhoon Cho),최의인(Euiin Choi) 한국멀티미디어학회 2007 한국멀티미디어학회 학술발표논문집 Vol.2007 No.1
최근 유ㆍ무선 인터넷 환경의 급성장과 유비쿼터스(Ubiquitous)라는 용어가 동장하면서 새로운 컴퓨팅 환경을 이용하여 개인화된 서비스를 제공하기 위해 많은 연구가 진행되고 있다. 현재 여행정보 제공서비스는 관광안내소와 문헌정보 제공과 인터넷을 사용한 웹 서비스 등의 방법으로 제공되고 있으나, 유비쿼터스 환경에서는 상황정보를 이용하여 사용자에게 개인화된 여행정보를 제공해 줄 수 있다. 이 때, 여행자들은 자신의 선호도와 현재 상황에 적절한 여행정보를 제공받길 원하게 되며, 여행자의 주변 상황 및 개인 선호도에 따라 다양하게 변화할 수 있는 여행정보를 정확히 제공하기 위해서는 개인의 성향을 파악하고 다양한 상황정보 중 유용한 상황정보만을 추출하는 기법이 요구된다. 본 논문에서는 유비쿼터스 환경에서 사용자의 상황정보를 기반으로 여행정보 서비스를 제공하고자 할 때 사용자에게 적절한 정보를 제공하기 위하여, 다양한 상황정보들 사이에서 유용한 정보를 추출할 수 있는 상황정보 필터링 기법을 제안한다.
Occurrence of Bemisia tabaci and its vectored virus in Korea
Minho Lee,Ki-Baik Uhm,Sunyoung Lee,Heeyong Park,Murad Ghanim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10
Nationwide occurrence of Bemisia tabaci Q biotype was identified from 2005 May to 2007 Dec. in total 28 cities/counties of 9 provinces such as Goyang (Kyung-gi), Gangnung (Gang-won), Jincheon (Chung-buk), Buyeo (Chung-nam), Seongju (Kyung-buk), Geoje (Kyung-nam), Bukjeju (Jeju). Host plants of the scale of B. tabaci Q biotype were over 15 crops of tomato, sweet pepper, hot pepper, eggplant, etc. and total 12 species of weeds such as Veronica persica, Ipomoea lacunosa, Conyza sumatrensis, I. hederacea, Xanthium canadense, Humulus japonicus, Boehmeria nivea, Artemisia vulgaris, Paederia scandens, Acalypha austeralis, Brassica juncea, Rumex crispus. For molecular identifying Bemisia tabaci B and Q biotypes, and Trialeurodes vaporariorum, for which it is difficult to distinguish morphologically, sequences of mitochondrial 16S rDNA and CO I (Cytochromoxidase I) gene were analyzed and restriction enzymes were selected for biotypespecific cleaved bands. As the results, Hinf I for 16S rDNA and Vsp I for CO I gene made specific band patterns for the B and Q biotypes in gel electrophoresis. Thus these methods were able to identify those biotypes and species without DNA sequence analysis. Populations of the Q biotype were collected in each regions of Korea from 2005 to 2007, and they were genetically compared using CO I gene sequences. Thus the populations were divided by three different groups which were introduced over 3-4 times before 2007 from different population sources. Geoje and Jeju were suggested as the first regions of introduction. Especially the populations in the first introduced regions were highly homologous with the Q biotype of Japan. In addition, infection pattern of secondary symbionts in populations of the B and Q biotypes in Korea were different from the Israeli populations. Thus it is suggested that Japan is the main source of B. tabaci Q biotype introduced to Korea. In addition, populations of the both of B and Q biotype in Korea were infected by Haemiltonella, which is more effectively related to the transmission of tomato yellow leaf curl begomovirus (TYLCV). Therefore it is needed to monitor continuously if the outbreak of begomovirus vectored by B. tabaci. In this molecular phylogenetic analysis, it was shown that the population of B. tabaci Q biotype in weed plants near greenhouse was introduced to crop plants in greenhouse. Therefore we understand that weed control is important to inhibit recurrence of B. tabaci in greenhouse. Three species of begomovirus, sweet potato leaf curl virus (SPLCV), tobacco leaf curl virus (TLCV), and TYLCV, were reported after introduction of B. tabaci in Korea. Especially Korean government removed all plants in the first TYLCV-occurred greenhouse in 2008. Multiplex PCR diagnosis between TLCV and TYLCV was developed for the more rapid and accurate monitoring. TLCV and TYLCV strains occurred in Korea were highly homologous with strains of Japan. Therefore these results support our suggestion that Japan is the main source of B. tabaci Q biotype introduced to Korea.