Effect of turmeric (Curcuma Longa), fenugreek (Trigonella foenum-graecum L.) and/or bioflavonoid supplementation to the broiler chicks diet and drinking water on the growth performanceand intestinal morphometeric parameters

Hassan Abdel-Rahman Abdel-Rahm,Said Ibrahim Fathalla,Mohamed Atef Helal,Abeer Abdel-aleem Nafeaa,Ibrahim Selim Zahran 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Alternative Treatment for Facial Nerve Paralysis in a Dog

Hassan Abdel-Rahman Abdel-Rahman,전형규,송근호,강준구,김덕환 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.6

A 4-year-old male Maltese dog was referred with chief complaint of facial nerve paralysis and hyperthermia. These clinical signs were occurred after tooth extraction. Leukocytosis and swelling of left side of gums were detected. He was medicated with antibiotics for 9 days, however, inability of blinking in left eye, lacrimation and hyperthermia were not improved. The patient was administrated with Oyaksungisan (50 mg/kg, PO, BID) for 14 days and was treated by injection-acupuncture (AP) with bee venom (200 μg/head, two times/week, total three times). The patient was treated by injection-AP with dexamethasone (1 mg/kg, two times/week, total two times). As a result, Left blepharon was slightly blinked at session 4. Blinking of left blepharon became normal after session 5. The present patient was a case with canine facial nerve paralysis which showed favorable therapeutic response by alternative treatment.

Gene Expression Profiling of Non-Hodgkin Lymphomas

Zekri, Abdel-Rahman Nabawy,Hassan, Zeinab Korany,Bahnassy, Abeer Ahmed,Eldahshan, Dina Hassan,El-Rouby, Mahmoud Nour Eldin,Kamel, Mahmoud Mohamed,Hafez, Mohamed Mahmoud Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Background: Chromosomal translocations are genetic aberrations associated with specific non-Hodgkin lymphoma (NHL) subtypes. This study investigated the differential gene expression profile of Egyptian NHL cases based on a microarray approach. Materials and Methods: The study included tissue samples from 40 NHL patients and 20 normal lymph nodes used as controls. Total RNA was extracted and used for cDNA microarray assays. The quantitative real time polymerase chain reaction was used to identify the aberrantly expressed genes in cancer. Results: Significant associations of 8 up-regulated and 4 down-regulated genes with NHL were observed. Aberrant expression of a new group of genes not reported previously was apparent, including down-regulated NAG14 protein, 3 beta hydroxy-delta 5-c27 steroid oxi-reductase, oxi-glutarate dehydrogenase (lipo-amide), immunoglobulin lambda like polypeptide 3, protein kinase x linked, Hmt1, and caveolin 2 Tetra protein. The up-regulated genes were Rb binding protein 5, DKFZP586J1624 protein, protein kinase inhibitor gamma, zinc finger protein 3, choline ethanolamine phospho-transferase CEPT1, protein phosphatase, and histone deacetylase-3. Conclusions: This study revealed that new differentially expressed genes that may be markers for NHL patients and individuals who are at high risk for cancer development.

Molecular Prognostic Profile of Egyptian HCC Cases Infected with Hepatitis C Virus

Zekri, Abdel-Rahman N.,Hassan, Zeinab K.,Bahnassy, Abeer A.,Sherif, Ghada M.,ELdahshan, Dina,Abouelhoda, Mohamed,Ali, Ahmed,Hafez, Mohamed M. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Background: Hepatocellular carcinoma (HCC) is a common and aggressive malignancy. Despite of the improvements in its treatment, HCC prognosis remains poor due to its recurrence after resection. This study provides complete genetic profile for Egyptian HCC. Genome-wide analyses were performed to identify the predictive signatures. Patients and Methods: Liver tissue was collected from 31 patients with diagnosis of HCC and gene expression levels in the tumours and their adjacent non-neoplastic tissues samples were studied by analyzing changes by microarray then correlate these with the clinico-pathological parameters. Genes were validated in an independent set by qPCR. The genomic profile was associated with genetic disorders and cancer focused on gene expression, cell cycle and cell death. Molecular profile analysis revealed cell cycle progression and arrest at G2/M, but progression to mitosis; unregulated DNA damage check-points, and apoptosis. Result: Nine hundred fifty eight transcripts out of the 25,000 studied cDNAs were differentially expressed; 503 were up-regulated and 455 were down-regulated. A total of 19 pathways were up-regulated through 27 genes and 13 pathways were down-regulated through 19 genes. Thirty-seven genes showed significant differences in their expression between HCC cases with high and low Alpha Feto Protein ($AFP{\geq}600$ IU/ml). The validation for the microarray was done by real time PCR assay in which PPP3CA, ATG-5, BACE genes showed down-regulation and ABCG2, RXRA, ELOVL2, CXR3 genes showed up-regulation. cDNA microarrays showed that among the major upregulated genes in HCC are sets. Conclusion: The identified genes could provide a panel of new diagnostic and prognostic aids for HCC.

Differentially Expressed Genes in Metastatic Advanced Egyptian Bladder Cancer

Zekri, Abdel-Rahman N,Hassan, Zeinab Korany,Bahnassy, Abeer A,Khaled, Hussein M,El-Rouby, Mahmoud N,Haggag, Rasha M,Abu-Taleb, Fouad M Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Bladder cancer is one of the most common cancers worldwide. Gene expression profiling using microarray technologies improves the understanding of cancer biology. The aim of this study was to determine the gene expression profile in Egyptian bladder cancer patients. Materials and Methods: Samples from 29 human bladder cancers and adjacent non-neoplastic tissues were analyzed by cDNA microarray, with hierarchical clustering and multidimensional analysis. Results: Five hundred and sixteen genes were differentially expressed of which SOS1, HDAC2, PLXNC1, GTSE1, ULK2, IRS2, ABCA12, TOP3A, HES1, and SRP68 genes were involved in 33 different pathways. The most frequently detected genes were: SOS1 in 20 different pathways; HDAC2 in 5 different pathways; IRS2 in 3 different pathways. There were 388 down-regulated genes. PLCB2 was involved in 11 different pathways, MDM2 in 9 pathways, FZD4 in 5 pathways, p15 and FGF12 in 4 pathways, POLE2 in 3 pathways, and MCM4 and POLR2E in 2 pathways. Thirty genes showed significant differences between transitional cell cancer (TCC) and squamous cell cancer (SCC) samples. Unsupervised cluster analysis of DNA microarray data revealed a clear distinction between low and high grade tumors. In addition 26 genes showed significant differences between low and high tumor stages, including fragile histidine triad, Ras and sialyltransferase 8 (alpha) and 16 showed significant differences between low and high tumor grades, like methionine adenosyl transferase II, beta. Conclusions: The present study identified some genes, that can be used as molecular biomarkers or target genes in Egyptian bladder cancer patients.

Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals

Marwa M. Bedier,Ahmed Abdel Rahman Hashem,Yosra M. Hassan 대한치과보존학회 2018 Restorative Dentistry & Endodontics Vol.43 No.4

Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of 50 µm inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/ XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of 50 µm in the dentinal tubules.

MicroRNAs and Metastasis-related Gene Expression in Egyptian Breast Cancer Patients

Hafez, Mohamed M.,Hassan, Zeinab K.,Zekri, Abdel Rahman N.,Gaber, Ayman A.,Rejaie, Salem S. Al,Sayed-Ahmed, Mohamed M.,Shabanah, Othman Al Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Aim and background: MicroRNAs (miRNAs) are a class of naturally occurring small noncoding RNAs that regulate gene expression, cell growth, differentiation and apoptosis by targeting mRNAs for translational repression or cleavage. The present study was conducted to study miRNAs in Egyptian breast cancer (BC) and their relation to metastasis, tumor invasion and apoptosis in addition to their association with the ER and PR statuses. Methods: Real Time RT-PCR was performed to identify the miRNA expression level of eight miRNAs and eight metastatic-related genes in 40 breast cancer samples and their adjacent non-neoplastic tissues. The expression levels of each miRNA relative to U6 RNA were determined using the $^{2-{\Delta}}CT$ method. Also, miRNA expression profiles of the BC and their corresponding ANT were evaluated. Results: The BC patients showed an up-regulation in miRNAs (mir-155, mir-10, mir-21 and mir-373) with an upregulation in MMP2, MMp9 and VEGF genes. We found down regulation in mir-17p, mir-126, mir-335, mir-30b and also TIMP3, TMP1 and PDCD4 genes in the cancer tissue compared to the adjacent non-neoplastic tissues. Mir -10b, mir -21, mir-155 and mir373 and the metastatic genes MMP2, MMP9 and VEGF were significantly associated with an increase in tumor size (P < 0.05). No significant difference was observed between any of the studied miRNAs regarding lymph node metastasis. Mir-21 was significantly over-expressed in ER-/PR-cases. Conclusion: Specific miRNAs (mir-10, mir-21, mir-155, mir-373, mir-30b, mir-126, mir-17p, mir-335) are associated with tumor metastasis and other clinical characteristics for BC, facilitating identification of individuals who are at risk.

Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals

Bedier, Marwa M.,Hashem, Ahmed Abdel Rahman,Hassan, Yosra M. The Korean Academy of Conservative Dentistry 2018 Restorative Dentistry & Endodontics Vol.43 No.4

Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of $50{\mu}m$ inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of $50{\mu}m$ in the dentinal tubules.

An Efficient Technique for Non-Uniformity Correction of Infrared Video Sequences with Histogram Matching

Abbass Mohammed Y.,Sadic Nevein,Ashiba Huda I.,Hassan Emad S.,El-Dolil Sami,Soliman Naglaa F.,Algarni Abeer D.,Alabdulkreem Eatedal A.,Algarni Fatimah,El-Banby Ghada M.,Abdel-Rahman Mohamed R.,Aldosar 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

Infrared (IR) image sequences are acquired with certain types of cameras. These cameras give the sequence of images according to the heat distribution. With time, some deterioration of the quality of the sequence occurs due the thermal noise eff ect generated in the camera. This thermal noise eff ect leads to some sort of non-uniformity in the obtained image sequence. Hence, it is necessary to perform some sort of non-uniformity correction in the video sequence according to the fi rst frame. This type of non-uniformity correction is scene-based. This paper introduces a scene-based non-uniformity correction technique that depends mainly on histogram matching. The noise eff ect on each frame in the sequence leads to some drift in the histogram of that frame. Hence, the proposed technique depends on the histogram matching concept to correct the histogram of each frame in the sequence based on the histogram of the fi rst frame that is free from the thermal noise eff ect. Diff erent image quality metrics including entropy, contrast, edge intensity, average gradient, and correlation with the fi rst frame are adopted to assess the quality of the obtained frames after adjustment. It is required in the frames to be corrected to reduce entropy, edge intensity and average gradient as these metrics are increased with the presence of thermal noise eff ect on all pixels represented as much details and unnecessary information. In addition, the contrast of the video sequences should be increased to determine objects in a better way. The correlation of the corrected frames with the fi rst one should be increased to reduce the noise eff ect. Simulation results reveal enhanced quality of the obtained video sequences after processing with the proposed technique.

In vivo effects of different anesthetic agents on apoptosis

Emad S. Osman,Hanan F. Khafagy,,Yasser M. Samhan,Mona M. Hassan,Faten M. El-Shanawany,Abdel Rahman M. Fathallah,Gehan G. El-fandy 대한마취통증의학회 2012 Korean Journal of Anesthesiology Vol.63 No.1

Background: This study was designed to measure in vivo effects of propofol, isoflurane and sevoflurane on apoptosis by measuring caspase-3 and tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) blood level as apoptotic markers. Methods: After obtaining ethical committee approval and informed written consents, sixty adult patients ASA I scheduled for open cholecystectomy participated in this study. They were randomally allocated into one of three equal groups to receive propofol infusion, low-flow isoflurane or sevoflurane for maintenance of anesthesia. Venous blood samples were collected preoperatively, immediately postoperative and after 24 hours to measure hemoglobin,hematocrit, creatinine, liver enzymes, serum TRAIL and caspase-3 levels. Results: There was no significant difference in hematological markers and serum creatinine. Liver enzymes showed significant postoperative rise (P < 0.05). In Propofol group, TRAIL and caspase-3 levels were significantly elevated immediately postoperative then decreased significantly after 24-hours (P < 0.05). In Isoflurane group, immediate postoperative level of TRAIL was significantly higher than 24 hours reading and significantly lower than its level in Propofol group at the same timing meanwhile caspase-3 levels were comparable at different timings. In Sevoflurane group, TRAIL and caspase-3 levels increased significantly in both postoperative samples than preoperative level and than those of Isoflurane and Propofol groups after 24 hours concerning TRAIL (P < 0.05). Conclusions: This study concluded that isoflurane is superior and sevoflurane is the least effective among the three anesthetics in protection against apoptosis. This study neither proved nor excluded propofol-induced apoptosis. Further studies are required during lengthy procedure and in compromised patients. Background: This study was designed to measure in vivo effects of propofol, isoflurane and sevoflurane on apoptosis by measuring caspase-3 and tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) blood level as apoptotic markers. Methods: After obtaining ethical committee approval and informed written consents, sixty adult patients ASA I scheduled for open cholecystectomy participated in this study. They were randomally allocated into one of three equal groups to receive propofol infusion, low-flow isoflurane or sevoflurane for maintenance of anesthesia. Venous blood samples were collected preoperatively, immediately postoperative and after 24 hours to measure hemoglobin,hematocrit, creatinine, liver enzymes, serum TRAIL and caspase-3 levels. Results: There was no significant difference in hematological markers and serum creatinine. Liver enzymes showed significant postoperative rise (P < 0.05). In Propofol group, TRAIL and caspase-3 levels were significantly elevated immediately postoperative then decreased significantly after 24-hours (P < 0.05). In Isoflurane group, immediate postoperative level of TRAIL was significantly higher than 24 hours reading and significantly lower than its level in Propofol group at the same timing meanwhile caspase-3 levels were comparable at different timings. In Sevoflurane group, TRAIL and caspase-3 levels increased significantly in both postoperative samples than preoperative level and than those of Isoflurane and Propofol groups after 24 hours concerning TRAIL (P < 0.05). Conclusions: This study concluded that isoflurane is superior and sevoflurane is the least effective among the three anesthetics in protection against apoptosis. This study neither proved nor excluded propofol-induced apoptosis. Further studies are required during lengthy procedure and in compromised patients.