Assessment of the Prognostic Value of Methylation Status and Expression Levels of FHIT, GSTP1 and p16 in Non-Small Cell Lung Cancer in Egyptian Patients

Haroun, Riham Abdel-Hamid,Zakhary, Nadia Iskandar,Mohamed, Mohamed Ragaa,Abdelrahman, Abdelrahman Mohamed,Kandil, Eman Ibrahim,Shalaby, Kamal Ali Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Background: Methylation of tumor suppressor genes has been investigated in all kinds of cancer. Tumor specific epigenetic alterations can be used as a molecular markers of malignancy, which can lead to better diagnosis, prognosis and therapy. Therefore, the aim of this study was to evaluate the association between gene hypermethylation and expression of fragile histidine triad (FHIT), glutathione S-transferase P1 (GSTP1) and p16 genes and various clinicopathologic characteristics in primary non-small cell lung carcinomas (NSCLC). Materials and Methods: The study included 28 primary non-small cell lung carcinomas, where an additional 28 tissue samples taken from apparently normal safety margin surrounding the tumors served as controls. Methylation-specific polymerase chain reaction (MSP) was performed to analyze the methylation status of FHIT, GSTP1 and p16 while their mRNA expression levels were measured using a real-time PCR assay with SYBR Green I. Results: The methylation frequencies of the genes tested in NSCLC specimens were 53.6% for FHIT, 25% for GSTP1, and 0% for p16, and the risk of FHIT hypermethylation increased among patients with NSCLC by 2.88, while the risk of GSTP1 hypermethylation increased by 2.33. Hypermethylation of FHIT gene showed a highly significant correlation with pathologic stage (p<0.01) and a significant correlation with smoking habit and FHIT mRNA expression level (p<0.05). In contrast, no correlation was observed between the methylation of GSTP1 or p16 and smoking habit or any other parameter investigated (p>0.05). Conclusions: Results of the present study suggest that methylation of FHIT is a useful biomarker of biologically aggressive disease in patients with NSCLC. FHIT methylation may play a role in lung cancer later metastatic stages while GSTP1 methylation may rather play a role in the early pathogenesis.

Population Monitoring of Pine Processionary Moth (Thaumetopoea pityocampa) by Pheromone Trapping at the Southern Limit of Distribution of Pinus halepensis in Eastern Algeria

( Chenchouni Haroun ),( Zanati Kamel ),( Rezougui Abderrahmane ),( Briki Athmane ),( Arar Abdelkrim ) 한국산림과학회 2010 Forest Science And Technology Vol.6 No.2

We monitored emergence dates of adults in order to assess local population density and to determine the life cycle of the Pine Processionary Moth (Thaumetopoea pityocampa Denis & Schif.). A series of 126 pheromone traps were placed randomly on trunks of Aleppo pine (Pinus halepensis) located in the major forests of Batna City (North-eastern Algeria). We harvested daily contents of traps installed at an average height of 1.70±0.30 m, where a total of 19,339 males were captured from July 2008 to August 2008 with an average catch of 153.5±179.4 per trap. Thus, pheromone traps provided an important tool to assess population density and rhythms of adult flight. In addition, different findings are discussed such as main factors influencing adult emergence. This study was based on the use of mating disruption by mass trapping to limit future outbreaks on the one hand, and to provide data on the exact dates of adult emergence to monitor future outbreaks and to use the appropriate control technique on the other hand. Canonical correspondence analysis (CCA) showed that site variables that best explained the data were tree age and average stand height. CCA analysis indicated that altitude was not significant parameter. Factors leading to late adult emergence as well as PPM low densities in some forest stations are discussed.

Contact Dermatitis due to Lubricant Oils in a Brass Musician

( Elisa Haroun-diaz ),( Rita Rodrigues-barata ),( Javier Cuesta-herranz ),( Luis Conde-salazar ) 대한피부과학회 2017 Annals of Dermatology Vol.29 No.1

Preparation of Polyurethane Silicon Oxide Nanomaterials as a Binder in Leather Finishing

Hamed Elsayed,Rasha Attia,Ola Mohamed,Ahmed Haroun,Nabil El-Sayed 한국섬유공학회 2018 Fibers and polymers Vol.19 No.4

Leather finishing processes using toxic organic solvent based produce volatile organic compounds (VOC), chronic exposure to this chemicals effect on workers' health causing many diseases especially lung cancer. So, polyurethane waterbased was synthesized for application in leather finishing instead of organic solvent based because it’s economic and safety for industry and workers. Preparation of water-based polyurethane (PU) depends on the reaction of polyethylene glycol (PEG, 300) with isophorone diisocyanate (IPDI) and the reaction of IPDI-1,4-butanediol (BDO) together with dimethylolpropionic acid (DMPA), was synthesized by poly-addition polymerization reaction. PU was then modified with different amounts of silicon dioxide nanoparticles (1-5 % SiO2), used as a binder in leather finishing. Leather coated was characterized physically, chemically and thermally by FTIR, GPC, DLS, TEM, SEM and TGA. The results revel that, water vapor permeability (WVP) of leather coated with PU modified with SiO2 showed improvement due to the existence of SiO2 particles which increases the interspaces of the polyurethane coating. SEM showed that when the amount of SiO2 nanoparticles increases, there is uniform nanoparticles accumulated can be observed. EDX prove the presence of Si and O2 elements and the formation of SiO2 nanoparticles. Mechanical properties discussed that tensile strength; tear strength and elongation at break % increase with increase SiO2 concentration until 3 % SiO2 nanoparticles. TGA showed an improvement of thermal stability of coated leather modified with SiO2. Therefore, this study succeeded in preparation of safe, ecofriendly of water-based polyurethane binders which modified with SiO2 for using in leather finishing.

Diversity and phenology of hoverflies (Diptera: Syrphidae) in pine forests (Pinus halepensis Miller) of Algeria

Sihem Djellab,Nadjoua Mebarkia,Souad Neffar,Haroun Chenchouni 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Hoverflies are good indicators of ecosystem integrity, especially in drylands. However, the key factors explaining hoverfly diversity in North African forest ecosystems are still not addressed. The current study provides data on the diversity, structure and functional trophic groups (FTG) of the hoverfly community in Aleppo pine forests under a semi-arid climate in northeastern Algeria. Using an entomological net, hoverflies were sampled weekly during 2008–2009. Alpha and beta-diversity of hoverflies and functional trophic group (FTG) were analyzed using several parameters and indices (e.g. species composition, richness, occurrence, diversity, estimations, similarity, etc.). In total, 602 individuals of 21 species were collected with a constant species (Eupeodes corollae) and four common species (Episyrphus balteatus, Chrysotoxum intermedium, Eristalis arbustorum and Eristalis tenax). Most species (17) occurred accidentally or very accidentally in samples. The highest diversity was recorded during spring, corresponding to the flowering season of most understory plant species. Seasonal rarefaction and extrapolation curves indicated that the expected species richness would be higher in autumn and spring compared to summer and winter. The spectrum of FTG ranked predators first with 52.4% of species, followed by saprophagous (42.8%) and then phytophagous (4.8%) species. Hoverfly communities showed high taxonomic richness and alpha-diversity all over the year, with peaks during spring that coincides with flowering period of most plant species of the forest understorey and favourable climatic conditions.

Effect of FRP composites on buckling capacity of anchored steel tanks

H. Ramadan,M.A. Al-Kashif,A. Rashed,M.A. Haroun 국제구조공학회 2010 Steel and Composite Structures, An International J Vol.10 No.4

Enhancement in the seismic buckling capacity of steel tanks caused by the addition of fiber reinforced polymers (FRP) retrofit layers attached to the outer walls of the steel tank is investigated. Threedimensional non-linear finite element modeling is utilized to perform such analysis considering non linear material properties and non-linear large deformation large strain analysis. FRP composites which possess high stiffness and high failure strength are used to reduce the steel hoop stress and consequently improve the tank capacity. A number of tanks with varying dimensions and shell thicknesses are examined using FRP composites added in symmetric layers attached to the outer surface of the steel shell. The FRP shows its effectiveness in carrying part of the hoop stresses along with the steel before steel yielding. Following steel yielding, the FRP restrains the outward bulging of the tank and continues to resist higher hoop stresses. The percentage improvement in the ultimate base moment capacity of the tank due to the addition of more FRP layers is shown to be as high as 60% for some tanks. The percentage of increase in the tank moment capacity is shown to be dependent on the ratio of the shell thickness to the tank radius (t/R). Finally a new methodology has been explained to calculate the location of Elephant foot buckling and consequently the best location of FRP application.

HEMATOLOGICAL RESPONSE OF SAUDI ARABIAN FOWL TO PROTEIN REARING REGIMENS

Alsobayel, A.A.,Attia, F.M.,Bayoumi, M.S.,Haroun, I.Y. Asian Australasian Association of Animal Productio 1990 Animal Bioscience Vol.3 No.2

The purpose of this investigation was to study the hematological response of Saudi Arabian Baladi fowl to protein rearing regimens. Males and females were subjected to the following 4 protein rearing regimens: conventional, C; reverse protein, RP; 2 single-stage low protein, $SS_1$ and $SS_2$ using 15% and 12% CP diets, respectively. Regimen effect was highly significant ($$p{\leq_-}.01$$) on BW, PCY, TP and U-Ac and significant ($$p{\leq_-}.05$$) on TL. Serum chol levels were not affected by regimen. In general $SS_{2}$ birds showed the lowest values for all parameters studied, except for PCV. However, the differences were not significant in each case. Age and sex effects were highly significant ($$p{\leq_-}.01$$) for all parameters, however, the regimen X sex interaction was not significant except for PCV. Regimen X age interaction, on the other hand, was highly significant ($$p{\leq_-}.01$$) only for BW, TP and U-Ac concentrations. The data may suggest that low levels of protein in the rearing regimen is an important factor influencing levels of the blood parameters studied. The data also indicate a lack of clear relationship between hen-day egg production and the blood parameters studied.

Ethnobotanical importance of the endemic taxa in the Egyptian flora

El-Khalafy Mohamed Mahmoud,Ahmed Dalia Abd El-Azeem,Shaltout Kamal Hussein,Haroun Soliman Abdelfattah,Al-Sodany Yassin Mohamed 한국생태학회 2023 Journal of Ecology and Environment Vol.47 No.3

Background: Endemic species are important components in the flora of most world regions. Most of these species have become threatened and exposed to extinction within the last few years. The present study aims to evaluate the ecosystem services offered by the endemic plant taxa in Egypt and the threats that affect them. Twenty-five field visits were conducted during summer 2018 to spring 2022 to several locations all over Egypt. In each location, the main habitats, national distribution, abundance, goods and threats were recorded. Results: Egypt has 41 endemic taxa belonging to 36 genera and 20 families inhabiting 10 main habitats. Rocky surfaces and sandy formations have the highest number of endemic species. The relation between the number of endemic taxa and the abundance categories indicated that 2 taxa are rare (4.9% of the total taxa), while the remaining were very rare (95%). The most represented offered good was the medicinal uses (32 taxa = 78%), while fuel plants were only represented by 2 taxa (2 taxa = 4.9%). Besides, 14 taxa (34.1% of the total studied taxa) have at least 1 environmental service. Soil fertility (7 taxa = 50%) was the most represented, followed by sand accumulations (6 taxa out of 14 taxa = 43%), while shading plant was the least (1 taxon = 7.1%) (Rosa arabica). The most represented threat is over-cutting and over-collecting (38 taxa = 92.7%), while mining and quarrying is the least represented (4 taxa = 9.8%). Conclusions: The potential and actual goods, services and threats of the endemic taxa were assessed as follows; field observation, information collected from local inhabitants and herbalists, and a literature review. The present study recommended planning a strategy about the importance, threats and conservation of endemic taxa in Egypt that would help in the protection and rescue of these plants and increase awareness about the importance of these plants.

Clavulanic Acid Is a Leading Culprit Beta-Lactam in Immediate Allergic Reactions to Penicillins

Torres-Rojas Isabel,Pérez-Alzate Diana,Somoza Maria Luisa,Pfeifer Ana Prieto-Moreno,Diaz Elisa Haroun,Jimenez-Rodriguez Teodorikez W,Sánchez Javier Fernández,Ruano Francisco J,Blanca Miguel,Blanca-Lóp 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.2

Purpose: Clavulanate, a beta-lactam associated with amoxicillin, is frequently prescribed in patients at all ages. Recent data implicate amoxicillin-clavulanate in up to 80% of beta-lactam allergy cases. We assessed clavulanate’s role in inducing allergic reactions to this combination treatment, with a focus on selective immediate reactions. Methods: Adults (≥ 16 years) reporting a history of immediate reactions to amoxicillin-clavulanate were evaluated through a beta-lactam allergological workup, using modified European Academy of Allergy and Clinical Immunology guidelines. Patients first underwent skin testing, and if negative, drug provocation tests. Expected outcomes were: Group A, subjects with immediate reaction to classical penicillin group determinants (penicilloyl polylysine, minor determinants mixture, and/or penicillin G); Group B, subjects with selective immediate reaction to amoxicillin; Group C, subjects with selective immediate reaction to clavulanate and Group D, those immediate reactions with co-sensitization to clavulanate plus penicillin group determinants or amoxicillin. Results: Of 1,170 included patients, 104 had immediate reactions: 36.5% to penicillin group determinants (Group A), 26.9% to amoxicillin (Group B), 32.7% to clavulanate (Group C), and 3.8% to clavulanate plus penicillin determinants or amoxicillin (Group D). Diagnosis was made by skin testing in 79%, 75% and 47% of the patients, respectively, in the first 3 groups (P < 0.001). Drug provocation tests were necessary to establish most other diagnoses. Anaphylaxis predominated over urticaria/angioedema in all groups. Conclusions: Selective immediate reactions to clavulanate accounted for over a third of cases with confirmed reactions after amoxicillin-clavulanate intake, with more than half experiencing anaphylaxis. Within this group, skin test sensitivity was below 50%. People taking amoxicillin-clavulanate may also be co-sensitized to both drugs.

Applying the OMOP Common Data Model to Facilitate Benefit-Risk Assessments of Medicinal Products Using Real-World Data from Singapore and South Korea

Hui Xing Tan,Desmond Chun Hwee Teo,이동윤,김청수,Jing Wei Neo,Cynthia Sung,Haroun Chahed,Pei San Ang,Doreen Su Yin Tan,박래웅,Sreemanee Raaj Dorajoo 대한의료정보학회 2022 Healthcare Informatics Research Vol.28 No.2

Objectives: The aim of this study was to characterize the benefits of converting Electronic Medical Records (EMRs) to acommon data model (CDM) and to assess the potential of CDM-converted data to rapidly generate insights for benefitriskassessments in post-market regulatory evaluation and decisions. Methods: EMRs from January 2013 to December 2016were mapped onto the Observational Medical Outcomes Partnership-CDM (OMOP-CDM) schema. Vocabulary mappingswere applied to convert source data values into OMOP-CDM-endorsed terminologies. Existing analytic codes used in a priorOMOP-CDM drug utilization study were modified to conduct an illustrative analysis of oral anticoagulants used for atrialfibrillation in Singapore and South Korea, resembling a typical benefit-risk assessment. A novel visualization is proposed torepresent the comparative effectiveness, safety and utilization of the drugs. Results: Over 90% of records were mapped ontothe OMOP-CDM. The CDM data structures and analytic code templates simplified the querying of data for the analysis. Intotal, 2,419 patients from Singapore and South Korea fulfilled the study criteria, the majority of whom were warfarin users. After 3 months of follow-up, differences in cumulative incidence of bleeding and thromboembolic events were observable viathe proposed visualization, surfacing insights as to the agent of preference in a given clinical setting, which may meaningfullyinform regulatory decision-making. Conclusions: While the structure of the OMOP-CDM and its accessory tools facilitatereal-world data analysis, extending them to fulfil regulatory analytic purposes in the post-market setting, such as benefit-riskassessments, may require layering on additional analytic tools and visualization techniques.