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Mo Shixun,Lv Zhipeng,Han Kunlun,Yuan Rongji,Yang Hao,Jiang Kunping,Liang Zhenshen 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.2
When the power system is disturbed or the operation mode is changed, the electromagnetic transient process will occur, which will lead to the high frequency transient component and aff ect the protection and control of the power system. Therefore, attention should be paid to the analysis of high-frequency transient components when modeling and analyzing the system model. In view of the fact that the symmetrical-component theory is not suitable for asymmetric transmission lines and the phasor method is not suitable for transient analysis and calculation, this paper uses the phase coordinate approach and Laplace transform to derive the node voltage equation of three-phase π type transmission lines in frequency domain, which can be used to calculate the transient response of transmission lines caused by the change of operation mode or disturbance. And vectorized NILT is used to calculate the time domain solution, which greatly improves the calculation speed. The calculated results are basically consistent with those of ATP-EMTP, and can fully refl ect the transient characteristics. This method lays a foundation for analyzing the infl uence of high frequency transient components on power system and fault analysis considering transient process.
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Production and Optimization of a Kiwi Pectin Methylesterase Inhibitor in Pichia pastoris GS115
Qian Liu,Wentao Xu,Shiwen Han,Dongyan Cao,Xiaoyun He,Kunlun Huang,Xiaohong Mei 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6
Gene sequence coding of a kiwi pectin methylesteraseinhibitor was optimized, synthesized, and expressedin Pichia pastoris GS115 based on P. pastoris preferredcodon usage. The expression level of the recombinantprotein (kwPMEI) increased by 89.74% after codonoptimization. Expression conditions of recombinant strainswere optimized. The highest production of kwPMEI wasachieved using 0.8% sorbitol (added every 24 h), 0.05%oleic acid (added at the beginning of induction), and 0.5%methanol (added every 12 h). kwPMEI was purified usingNi2+ chelating affinity chromatography and 17 mg of theprotein was harvested from 60 mL of a culture supernatant. Activity analysis showed that kwPMEI efficiently inhibitedthe activity of different plant PMEs. High expression levelsand purification of kwPMEI will promote applications infruit and vegetable juices.
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