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Jeong, Se-Jin,Kim, Sinai,Park, Jong-Gil,Jung, In-hyuk,Lee, Mi-Ni,Jeon, Sejin,Kweon, Hyae Yon,Yu, Dae-Yeul,Lee, Sang-Hak,Jang, Yangsoo,Kang, Sang Won,Han, Ki-Hwan,Miller, Yury I.,Park, Young Mi,Cheong, LANDES BIOSCIENCE 2018 AUTOPHAGY Vol.14 No.1
<P><B>ABSTRACT</B></P><P>Oxidative stress activates macroautophagy/autophagy and contributes to atherogenesis via lipophagic flux, a form of lipid removal by autophagy. However, it is not known exactly how endogenous antioxidant enzymes are involved in lipophagic flux. Here, we demonstrate that the antioxidant PRDX1 (peroxiredoxin 1) has a crucial role in the maintenance of lipophagic flux in macrophages. PRDX1 is more highly expressed than other antioxidant enzymes in monocytes and macrophages. We determined that <I>Prdx1</I> deficiency induced excessive oxidative stress and impaired maintenance of autophagic flux in macrophages. <I>Prdx1</I>-deficient macrophages had higher intracellular cholesterol mass and lower cholesterol efflux compared with wild type. This perturbation in cholesterol homeostasis was due to impaired lipophagic cholesterol hydrolysis caused by excessive oxidative stress, resulting in the inhibition of free cholesterol formation and the reduction of NR1H3 (nuclear receptor subfamily 1, group H, member 3) activity. Notably, impairment of both lipophagic flux and cholesterol efflux was restored by the 2-Cys PRDX-mimics ebselen and gliotoxin. Consistent with this observation, <I>apoe <SUP>−/−</SUP></I> mice transplanted with bone marrow from <I>prdx1<SUP>−/−</SUP>apoe<SUP>−/−</SUP></I> mice had increased plaque formation compared with <I>apoe<SUP>−/−</SUP></I> BM-transplanted recipients. This study reveals that PRDX1 is crucial to regulating lipophagic flux and maintaining macrophage cholesterol homeostasis against oxidative stress. We suggest that PRDX1-dependent control of oxidative stress may provide a strategy for treating atherosclerosis and autophagy-related human diseases.</P>
Kim, Sung Eun,Yun, Young-Pil,Shim, Kyu-Sik,Jeon, Daniel I.,Park, Kyeongsoon,Kim, Hak-Jun Elsevier 2018 Journal of industrial and engineering chemistry Vol.58 No.-
<P><B>Abstract</B></P> <P>Achilles tendinopathy is a debilitating musculoskeletal condition that causes significant pain and leads to tendon rupture. Cur/PMSs exerted anti-inflammatory effects on LPS-treated tenocytes in a dose-dependent manner by showing significant decreases in the expression of pro-inflammatory markers such as MMP-3 and MMP-13, COX-2, ADAMTS-5, IL-6, and TNF-α. We demonstrated that local injection of Cur/PMSs markedly suppressed the expression of pro-inflammatory markers in tendon tissue of rats with collagenase-induced Achilles tendinopathy. Cur/PMSs remarkably increased the tensile strength of tendon tissue in a dose-dependent manner. Our findings indicate that Cur/PMSs have great potential for tendon tissue healing and restoration in Achilles tendinopathy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The fabricated Cur/PMSs showed sustained release for 28 days. </LI> <LI> Cur/PMSs decreased the expression of pro-inflammatory cytokines in LPS-treated tenocytes. </LI> <LI> Local treatment of Cur/PMSs suppressed the progression of Achilles tendinopathy. </LI> <LI> Cur/PMSs markedly increased the tensile strength of tendon tissue. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Jae Yong,Kim, Sung Eun,Yun, Young-Pil,Choi, Sung-Wook,Jeon, Daniel I.,Kim, Hak-Jun,Park, Kyeongsoon,Song, Hae-Ryong Elsevier 2017 Journal of industrial and engineering chemistry Vol.52 No.-
<P><B>Abstract</B></P> <P>In this study, we evaluated <I>in vitro</I> osteogenic differentiation and <I>in vivo</I> new bone formation using the alendronate-releasing porous PLGA microsphere (Aln/PMS) system in rats with a critical-sized calvarial defect. Aln/PMS system significantly enhanced <I>in vitro</I> osteogenic differentiation and maturation, showing significantly enhanced ALP activity, calcium deposition and the expression of osteocalcin and osteopontin, relative to PMS alone. Also, micro-CT analyses and histology results suggested that Aln/PMS system increased mineralization and bone matrix formation compared to PMS alone. This study demonstrated that the local delivery of Aln, a potent-osteoinductive factor, significantly enhances rabbit adipose-derived stem cells osteogenesis and bone regeneration.</P>
Differentiaton of Bovine Lens Epithelial Cells in Vitro
Kim, Jong-Tak,Lee, Jin. Hak,Joo, Choun- K.i,Jung, kwang-H. oi,Lee, Kyung. H.un 가톨릭 의과학연구원 1997 가톨릭 의과학연구원 국제학술대회 Vol.1 No.-
Well differentiated BLECs have increased in size, contain numerous vacuoles, and product a lot of ECMs in vitro.
Circulating Tumor Cell Microseparator Based on Lateral Magnetophoresis and Immunomagnetic Nanobeads
Kim, Seonyoung,Han, Song-I,Park, Min-Jae,Jeon, Chang-Wan,Joo, Young-Don,Choi, In-Hak,Han, Ki-Ho American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.5
<P>This paper presents a circulating tumor cell (CTC) microseparator for isolation of CTCs from human peripheral blood using immunomagnetic nanobeads with bound antiepithelial cell adhesive molecule (EpCAM) antibodies that specifically bind to epithelial cancer cells. The isolation is performed through lateral magnetophoresis, which is induced by high-gradient magnetic separation technology, involving a ferromagnetic wire array inlaid in the bottom substrate of a microchannel. Experimental results showed that the CTC microseparator isolates about 90% of spiked CTCs in human peripheral blood at a flow rate of up to 5 mL/h and purifies to approximately 97%. The overall isolation procedure was completed within 15 min for 200 μL of peripheral blood. CTCs from peripheral blood of patients with breast and lung cancers were isolated with the CTC microseparator, and the results were compared with those of healthy donors. Using a fluorescence-based viability assay, the viability of CTCs isolated from peripheral blood of patients with cancer was observed. In addition, the usefulness of the CTC microseparator for subsequent genetic assay was confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR) amplification of cancer-specific genes using CTCs isolated from patients with cancer.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-5/ac303284u/production/images/medium/ac-2012-03284u_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac303284u'>ACS Electronic Supporting Info</A></P>
권학철(Hak Cheol Kwon),방은정(Eun Jung Bang),최상운(Sang Un Choi),이완주(Won Chu Lee),조세연(Sea Yun Cho),정이연(I Yeon Jung),김선여(Sun Yeou Kim),이강노(Kang Ro Lee) 대한약학회 2000 약학회지 Vol.44 No.2
From the MeOH extract of Bombycis corpus inoculated by Beauberia bassiana 101A, two cyclodepsipeptides, bassianolide (1) and beaubericin (2), were isolated and their structures were determined by one and two dimensional NMR studies. Compounds 1 and 2 exhibited cytotoxicity against cultured human tumor cell lines.