Melatonin protects endothelial progenitor L255cells against AGE-induced apoptosis via autophagy flux stimulation and promotes wound healing in diabetic mice

Haiming Jin,Zengjie Zhang,Chengui Wang,Qian Tang,Jianle Wang,Xueqin Bai,Qingqing Wang,Majid Nisar,Naifeng Tian,Quan Wang,Cong Mao,Xiaolei Zhang,Xiangyang Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Wound healing is delayed in diabetic patients. Increased apoptosis and endothelial progenitor cell (EPC) dysfunction are implicated in delayed diabetic wound healing. Melatonin, a major secretory product of the pineal gland, promotes diabetic wound healing; however, its mechanism of action remains unclear. Here, EPCs were isolated from the bone marrow of mice. Treatment of EPCs with melatonin alleviated advanced glycation end product (AGE)-induced apoptosis and cellular dysfunction. We further examined autophagy flux after melatonin treatment and found increased light chain 3 (LC3) and p62 protein levels in AGE-treated EPCs. However, lysosome-associated membrane protein 2 expression was decreased, indicating that autophagy flux was impaired in EPCs treated with AGEs. We then evaluated autophagy flux after melatonin treatment and found that melatonin increased the LC3 levels, but attenuated the accumulation of p62, suggesting a stimulatory effect of melatonin on autophagy flux. Blockage of autophagy flux by chloroquine partially abolished the protective effects of melatonin, indicating that autophagy flux is involved in the protective effects of melatonin. Furthermore, we found that the AMPK/mTOR signaling pathway is involved in autophagy flux stimulation by melatonin. An in vivo study also illustrated that melatonin treatment ameliorated impaired wound healing in a streptozotocin-induced diabetic wound healing model. Thus, our study shows that melatonin protects EPCs against apoptosis and dysfunction via autophagy flux stimulation and ameliorates impaired wound healing in vivo, providing insight into its mechanism of action in diabetic wound healing.

Small molecule natural compound agonist of SIRT3 as a therapeutic target for the treatment of intervertebral disc degeneration

Jianle Wang,Majid Nisar,Chongan Huang,Xiangxiang Pan,Dongdong Lin,Gang Zheng,Haiming Jin,Deheng Chen,Naifeng Tian,Qianyu Huang,Yue Duan,Yingzhao Yan,Ke Wang,Congcong Wu,Jianing Hu,Xiaolei Zhang,Xiangy 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Oxidative stress-induced mitochondrial dysfunction is implicated in the pathogenesis of intervertebral disc degeneration (IVDD). Sirtuin 3 (SIRT3), a sirtuin family protein located in mitochondria, is essential for mitochondrial homeostasis; however, the role of SIRT3 in the process of IVDD has remained elusive. Here, we explored the expression of SIRT3 in IVDD in vivo and in vitro; we also explored the role of SIRT3 in senescence, apoptosis, and mitochondrial homeostasis under oxidative stress. We subsequently activated SIRT3 using honokiol to evaluate its therapeutic potential for IVDD. We assessed SIRT3 expression in degenerative nucleus pulposus (NP) tissues and oxidative stressinduced nucleus pulposus cells (NPCs). SIRT3 was knocked down by lentivirus and activated by honokiol to determine its role in oxidative stress-induced NPCs. The mechanism by which honokiol affected SIRT3 regulation was investigated in vitro, and the therapeutic potential of honokiol was assessed in vitro and in vivo. We found that the expression of SIRT3 decreased with IVDD, and SIRT3 knockdown reduced the tolerance of NPCs to oxidative stress. Honokiol (10 μM) improved the viability of NPCs under oxidative stress and promoted their properties of anti-oxidation, mitochondrial dynamics and mitophagy in a SIRT3-dependent manner. Furthermore, honokiol activated SIRT3 through the AMPKPGC- 1α signaling pathway. Moreover, honokiol treatment ameliorated IVDD in rats. Our study indicated that SIRT3 is involved in IVDD and showed the potential of the SIRT3 agonist honokiol for the treatment of IVDD.

A Dislocation Density Based Crystal Plasticity Model of 3D Complex Sheet Metal Forming

Haiming Zhang,Xianghuai Dong,Qian Wang,Zhiying Chen 한국소성가공학회 2011 기타자료 Vol.2011 No.8

A dislocation density based crystal plasticity model is used to simulate sheet metal forming processes under different temperature. In the present model, dislocation densities in each slip system are taken as internal state variables. An extended Kocks-Mecking-Estrin evolution law is adopted to describe the athermal storage, and the strain rate and temperature sensitive annihilation, of dislocation densities. The critical resolved stress on a specified slip system is related with dislocation densities in all slip systems, the corresponding mesoscopic coefficients of interaction between different slip systems are determined by recent dislocation dynamics simulations. On the basis of thermally activated dislocation motion, this physically based model is capable of describing the softening resulted from dynamic recovery. This model incorporated with a hyperelastic constitutive law is then implemented into an explicit finite element code, to make it deal with the strong nonlinear problems more efficiently. An iteration-free semi-implicit method, which is more suitable for explicit FEM, is adopted as the integration algorithm of the model. This model is used to investigate the deformation characteristics of warm forming of Al-Mg alloy under different temperatures. The simulation results show that the model is robust and efficient, has the potential to be used in the practical engineering simulation. It also confirms that the micromechaincs models can be used to simulate conventional sheet metal forming.

Effects of short-term soil tillage management on activity and community structure of denitrifiers under double-cropping rice field

Haiming Tang,Chao Li,Kaikai Cheng,Lihong Shi,Li Wen,Xiaoping Xiao,Yilan Xu,Weiyan Li,Ke Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH4 +-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

Moment-rotation relationship of hollow-section beam-to-column steel joints with extended end-plates

Jia Wang,Haiming Zhu,Brian Uy,Vipulkumar Patel,Farhad Aslani,Dongxu Li 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.6

This paper presents the flexural performance of steel beam-to-column joints composed of hollow structural section beams and columns. A finite element (FE) model was developed incorporating geometrical and material nonlinearities to evaluate the behaviour of joints subjected to bending moments. The numerical outcomes were validated with experimental results and compared with EN1993-1-8. The demountability of the structure was discussed based on the tested specimen. A parametric analysis was carried out to investigate the effects of steel yield strength, end-plate thickness, beam thickness, column wall thickness, bolt diameter, number of bolts and location. Consequently, an analytical model was derived based on the component method to predict the moment-rotation relationships for the sub-assemblies with extended end-plates. The accuracy of the proposed model was calibrated by the experimental and numerical results. It is found that the FE model is fairly reliable to predict the initial stiffness and moment capacity of the joints, while EN1993-1-8 overestimates the initial stiffness extensively. The beam-to-column joints are shown to be demountable and reusable with a moment up to 53% of the ultimate moment capacity. The end-plate thickness and column wall thickness have a significant influence on the joint behaviour, and the layout of double bolt-rows in tension is recommended for joints with extended end-plates. The derived analytical model is capable of predicting the moment-rotation relationship of the structure.

Effects of vitamin A supplementation in the diet of breeding geese on offspring intestinal tissue morphology and immune performance

Yang Haiming,Liang Jingru,Dai Hang,Wan Xiaoli,Wang Zhiyue 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9

Objective: The effects of maternal and offspring dietary vitamin A (VA) supplementation on early body weight, digestive tract function and immune function in goslings were studied. Methods: Yangzhou geese (180 d old) were randomly divided into 5 experimental groups of 15 females and 3 males (the males were kept until slaughter). Eggs were collected for hatching during the peak laying period. A total of 96 goslings were selected from each treatment group (each fed a basic diet supplemented with 0, 4,000, 8,000, 12,000 or 16,000 IU/kg VA) and randomly divided into 2 groups, with 6 replicates in each group and 8 goslings in each replicate. The gosling diet was supplemented with 0 or 9,000 IU/kg VA. Results: i) Villus length, villus width and the muscle thickness of the duodenum, jejunum and ileum were increased and the crypt depth was reduced after adding 12,000 IU/kg VA to the goslings’ diet (p<0.05). Adding 9,000 IU/kg VA to the offspring diet increased the length of the duodenal villi and width of the ileum and decreased the crypt depth of the ileum (p<0.05). ii) Supplementing the maternal diet with 12,000 IU/kg VA increased immune organ weight, the immune organ index and immunoglobulin content in goslings (p<0.05). The bursa weight and immunoglobulin G content of offspring were higher in the 9,000 IU/kg VA supplementation group than in the group with no supplementation (p<0.05). Conclusion: Offspring growth and development were affected by the amount of VA added into maternal diet. The negative effect of maternal VA deficiency on offspring can be compensated by adding VA to the offspring diet. Continued VA supplementation in the offspring diet after excessive VA supplementation in the maternal diet is unfavorable for gosling growth and development.

Device-to-Device Communication Underlaying Cellular Networks: Connection Establishment and Interference Avoidance

( Shaoyi Xu ),( Haiming Wang ),( Tao Chen ),( Tao Peng ),( Kyung Sup Kwak ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

It is expected that device-to-device (D2D) communication is allowed to underlay future cellular networks such as IMT-Advanced for spectrum efficiency. This article studies the mechanisms of D2D communication and interference avoidance when the D2D subsystem reuses uplink resources and downlink spectrums with a cellular system, respectively. We firstly propose an effective scheme to establish and maintain D2D communication. Moreover, a novel method to deal with the resource allocation and interference avoidance issues by utilizing the network peculiarity of a hybrid network to share the uplink resource is proposed. Most research focuses on reusing the uplink spectrums, but how to share the downlink frequency bands is seldom addressed. To share the downlink spectrums and avoid the interference to the primary cellular devices, a labeled time slots based mechanism is proposed. Implementation details are described in a real cellular system and simulation results prove that satisfying performance can be achieved by using the proposed mechanisms.

An Anti-Overvoltage Strategy for Electrolytic Capacitorless IPMSM Drives Using Power Controller

Dawei Ding,Haiming Hu,Gaolin Wang,Guoqiang Zhang,Dianguo Xu,Frede Blaabjerg 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Three-phase input diode rectifier interior permanent magnet synchronous motor (IPMSM) drives with small-volume film capacitors have many merits, such as high reliability, low cost and high power density. However, in regenerative braking, the DC-link voltage becomes harder to be controlled than the traditional topology due to the application of slim film capacitors. In this paper, an anti-overvoltage strategy is proposed by controlling the electrical power of the motor directly. The q-axis current boundary is determined by the proposed power controller. Compared with the conventional method of voltage controller, the proposed method is more suitable for electrolytic capacitorless drives.

The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling

Li Ruyi,Wang Feiyun,Wei Jianghong,Lin Yun,Tang Guofang,Rao Lizong,Ma Libing,Xu Qing,Wu Jingjie,Lv Qian,Zhou Rui,Lei Huiren,Zhao Xueqiang,Yao Dong,Xiao Bo,Huang Haiming,Zhang Jiange,Mo Biwen 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.1

Purpose: Recent studies have demonstrated that macrophage migration inhibitory factor (MIF) is of importance in asthmatic inflammation. The role of MIF in modulating airway remodeling has not yet been thoroughly elucidated to date. In the present study, we hypothesized that MIF promoted airway remodeling by intensifying airway smooth muscle cell (ASMC) autophagy and explored the specific mechanisms. Methods: MIF knockdown in the lung tissues of C57BL/6 mice was conducted by instilling intratracheally adeno-associated virus (AAV) vectors (MIF-mutant AAV9) into mouse lung tissues. Mice genetically deficient in the autophagy marker ATG5 (ATG5+/−) was used to detect the role of autophagy in ovalbumin (OVA)-asthmatic murine models. Moreover, to block the expression of MIF and CD74 in vitro models, inhibitors, antibodies and lentivirus transfection techniques were employed. Results: First, MIF knockdown in the lung tissues of mice showed markedly reduced airway remodeling in OVA murine mice models. Secondly, ASMC autophagy was increased in the OVA-challenged models. Mice genetically deficient in the autophagy marker ATG5 (ATG5+/−) that were primed and challenged with OVA showed lower airway remodeling than genetically wild-type asthmatic mice. Thirdly, MIF can induce ASMC autophagy in vitro. Moreover, the cellular source of MIF which promoted ASMC autophagy was macrophages. Finally, MIF promoted ASMC autophagy in a CD74-dependent manner. Conclusions: MIF can increase asthmatic airway remodeling by enhancing ASMC autophagy. Macrophage-derived MIF can promote ASMC autophagy by targeting CD74.

Effects of dietary fiber levels on cecal microbiota composition in geese

Li, Yanpin,Yang, Haiming,Xu, Lei,Wang, Zhiyue,Zhao, Yue,Chen, Xiaoshuai Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: This study shows the effects of dietary fiber levels on cecal microbiota composition in geese at day 70 according to pyrosequencing of the 16S ribosomal RNA gene. Methods: A total of 468 1-day-old healthy male Yangzhou goslings with similar body weight were randomly divided into 3 groups with 6 replicates per group and 26 geese per replicate. Geese were fed diets with fiber levels of 2.5% (low fiber level diet, Group I) and 6.1% (Group III) during days 1-70, respectively, or 4.3% for days 1-28 and 6.1% for days 29-70 (Group II). Results: Low fiber level diet decreased body weight, average daily gain during, increased lower feed conversation rate of geese during day 1 to 70 (p<0.05). Low fiber level diet decreased the total operational taxonomic units, Chao1 index and Shannon index, whereas increased the Simpson index of cecal microbiota in geese at day 70. Low fiber level diet decreased the relative abundance of Bacteroidetes, Firmicutes, Bacteroides, and Paraprevotella in cecum of geese at day 70. The similarity of cecal microbiota between low fiber level diet group and other groups was smaller. Conclusion: This study indicates that the low fiber level diet decreased diversity of microbiota, and relative abundance of some beneficial microbiota in cecum of geese at day 70, implying that the low fiber level diet has negative influence on performance by altering the diversity and population of cecal microbiota in geese.