MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis

Yang Yang,Rongfang Qiu,Qiaoyou Weng,Ziwei Xu,Jingjing Song,Siyu Zhao,Miaomiao Meng,Dengke Zhang,Chunli Kong,Hailin Wang,Min Xu,Zhongwei Zhao,Jiansong Ji 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose Mixed-lineage leukemia protein 4 (MLL4/KMT2D) is a histone methyltransferase, and its mutation has been reported to be associated with a poor prognosis in many cancers, including lung cancer. We investigated the function of MLL4 in lung carcinogenesis. Materials and Methods RNA sequencing (RNA-seq) in A549 cells transfected with control siRNA or MLL4 siRNA was performed. Also, we used EdU incorporation assay, colony formation assays, growth curve analysis, transwell invasion assays, immunohistochemical staining, and in vivo bioluminescence assay to investigate the function of MLL4 in lung carcinogenesis. Results We found that MLL4 expression was downregulated in non–small cell lung cancer (NSCLC) tissues compared to adjacent normal tissues and tended to decrease with disease stage progression. We analyzed the transcriptomes in control and MLL4- deficient cells using high-throughput RNA deep sequencing (RNA-seq) and identified a cohort of target genes, such as SOX2, ATF1, FOXP4, PIK3IP1, SIRT4, TENT5B, and LFNG, some of which are related to proliferation and metastasis. Our results showed that low expression of MLL4 promotes NSCLC cell proliferation and metastasis and is required for the maintenance of NSCLC stem cell properties. Conclusion Our findings identify an important role of MLL4 in lung carcinogenesis through transcriptional regulation of PIK3IP1, affecting the PI3K/AKT/SOX2 axis, and suggest that MLL4 could be a potential prognostic indicator and target for NSCLC therapy.

Computationally Efficient 2-D DOA Estimation Using Two Parallel Uniform Linear Arrays

Hailin Cao,Lisheng Yang,Xiaoheng Tan,Shizhong Yang 한국전자통신연구원 2009 ETRI Journal Vol.31 No.6

A new computationally efficient algorithm-based propagator method for two-dimensional (2-D) direction-of-arrival (DOA) estimation is proposed, which uses two parallel uniform linear arrays. The algorithm takes advantage of the special structure of the array which enables 2-D DOA estimation without pair matching. Simulation results show that the proposed algorithm achieves very accurate estimation at a computational cost 4 dB lower than that of standard methods.

Repeatability of Tensile Properties in High Pressure Die-Castings of an Al-Mg-Si-Mn Alloy

Hailin Yang,Shouxun Ji,Douglas Watson,Zhongyun Fan 대한금속·재료학회 2015 METALS AND MATERIALS International Vol.21 No.5

High pressure die-castings of an Al-Mg-Si-Mn alloy have been assessed in terms of the repeatability of the mechanical properties including yield strength, ultimate tensile strength and elongation by the normal standard deviations method and by the Weibull statistical model with three parameters. It was found that the round samples had the maximum Weibull modulus, indicating the best repeatability. The machined samples exhibited the second best of Weibull modulus. Among the square samples, the 2 mm and 5 mm thick samples had the lowest and the highest Weibull modulus respectively, indicating that the repeatability for the castings was influenced by the wall thickness. The microstructural uniformity and porosity levels are critical factors in determining the repeatability of the high pressure die-castings. A less segregation in the microstructure could uniform the stress distribution in the die-castings and a less porosity in the casting could reduce the sources for brittle fracture. These improved the repeatability in casting production.

Synthesis and Characterization of WC-Co Nanosized Composite Powders With in situ Carbon and Gas Carbon Sources

Qiumin Yang,Jiangao Yang,Hailin Yang,Wei Su,Jianming Ruan 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.4

This study presented nanosized WC-Co composite powders synthesized using a one-step reduction-carbonization process with a combination of CH4/H2 as a gas carbon source and soluble starch as an in situ carbon source. The results of carbon analysis and X-ray diffraction revealed that WC-Co nanocomposite powders with a pure WC and Co phase could be obtained at 1100 °C after 0.5 h. A higher gas flow ratio of CH4/H2 during the reduction-carbonization process led to a higher total carbon content of the sample. A field emission scanning electron microscope confirmed that the particles in the WC-6 wt% Co composite powders had the lowest average size of 43 nm with equiaxed shapes. A sintering neck was observed in the WC-3 wt% Co composite powders whereas faceted particles were found in the WC-12 wt% Co composite powders. Moreover, this method has advantages of simple processing, rapid synthesis and good applicability in potential industry application.

Lack of Association Between the Matrix Metalloproteinase-2 -1306C>T Polymorphism and Breast Cancer Susceptibility: a Meta-analysis

Yang, Lu,Li, Ning,Wang, Siyu,Kong, Yanan,Tang, Hailin,Xie, Xinhua,Xie, Xiaoming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: Since inconsistent results have been reported regarding the relation between the matrix metalloproteinase-2 (MMP-2) -1306C>T polymorphism and susceptibility for breast cancer, we performed a meta-analysis to investigate the issue. Materials and Methods: An internet search of PubMed and EMBASE was performed to identify eligible studies. Pooled odds ratios (ORs) with their corresponding confidence intervals (CIs) were calculated to evaluate any association between MMP-2 -1306C>T polymorphism and breast cancer susceptibility. Results: Nine case-control studies were included in the meta-analysis, involving 9,858 cases and 10,871 controls. Overall, there was no evidence of any association between the MMP-2 -1306C>T polymorphism and breast cancer susceptibility in different genetic models (T-allele vs C-allele: OR=0.95, 95%CI, 0.82-1.10, p=0.49; TT vs CC: OR=1.03, 95%CI, 0.90-1.19, p=0.66; TT+TC vs CC: OR=0.93, 95%CI, 0.78-1.10, p=0.38; TT vs TC+CC: OR=1.02, 95%CI, 0.89-1.17, p=0.77). In the subgroup analysis by ethnicity, CC was associated with a significant increase in breast susceptibility among Latin-Americans in the dominant model (OR=0.61, 95%CI, 0.40-0.93, p=0.02), but the association disappeared in other models. No significant association was observed among Europeans, East Asians and others in different genetic models. In the subgroup analysis by their source of controls, no significant association between MMP-2 -1306C>T polymorphism and breast cancer susceptibility was noted among population-based studies and hospital-based studies in different genetic models. Conclusions: The results of this meta-analysis suggest that MMP-2 -1306C>T polymorphism is not associated with breast cancer susceptibility, although the association among Latin-Americans in the dominant model was significant.

Single Nanorod Devices for Battery Diagnostics: A Case Study on LiMn₂O₄

Yang, Yuan,Xie, Chong,Ruffo, Riccardo,Peng, Hailin,Kim, Do Kyung,Cui, Yi American Chemical Society 2009 NANO LETTERS Vol.9 No.12

<P>This paper presents single nanostructure devices as a powerful new diagnostic tool for batteries with LiMn<SUB>2</SUB>O<SUB>4</SUB> nanorod materials as an example. LiMn<SUB>2</SUB>O<SUB>4</SUB> and Al-doped LiMn<SUB>2</SUB>O<SUB>4</SUB> nanorods were synthesized by a two-step method that combines hydrothermal synthesis of β-MnO<SUB>2</SUB> nanorods and a solid state reaction to convert them to LiMn<SUB>2</SUB>O<SUB>4</SUB> nanorods. λ-MnO<SUB>2</SUB> nanorods were also prepared by acid treatment of LiMn<SUB>2</SUB>O<SUB>4</SUB> nanorods. The effect of electrolyte etching on these LiMn<SUB>2</SUB>O<SUB>4</SUB>-related nanorods is investigated by both SEM and single-nanorod transport measurement, and this is the first time that the transport properties of this material have been studied at the level of an individual single-crystalline particle. Experiments show that Al dopants reduce the dissolution of Mn<SUP>3+</SUP> ions significantly and make the LiAl<SUB>0.1</SUB>Mn<SUB>1.9</SUB>O<SUB>4</SUB> nanorods much more stable than LiMn<SUB>2</SUB>O<SUB>4</SUB> against electrolyte etching, which is reflected by the magnification of both size shrinkage and conductance decrease. These results correlate well with the better cycling performance of Al-doped LiMn<SUB>2</SUB>O<SUB>4</SUB> in our Li-ion battery tests: LiAl<SUB>0.1</SUB>Mn<SUB>1.9</SUB>O<SUB>4</SUB> nanorods achieve 96% capacity retention after 100 cycles at 1C rate at room temperature, and 80% at 60 °C, whereas LiMn<SUB>2</SUB>O<SUB>4</SUB> shows worse retention of 91% at room temperature, and 69% at 60 °C. Moreover, temperature-dependent <I>I</I>−<I>V</I> measurements indicate that the sharp electronic resistance increase due to charge ordering transition at 290 K does not appear in our LiMn<SUB>2</SUB>O<SUB>4</SUB> nanorod samples, suggesting good battery performance at low temperature.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2009/nalefd.2009.9.issue-12/nl902315u/production/images/medium/nl-2009-02315u_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl902315u'>ACS Electronic Supporting Info</A></P>

High Strength Vinegar Fermentation by Acetobacter pasteurianus via Enhancing Alcohol Respiratory Chain

Zhengliang Qi,Hailin Yang,Xiaole Xia,Wu Wang,Xiao-Bin Yu 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

Seeking high strength vinegar fermentation byacetic acid bacteria (AAB) is still the mission of vinegarproducers. AAB alcohol respiratory chain, located onintracellular membrane, is directly responsible for vinegarfermentation. In the semi-continuous vinegar fermentationby Acetobacter pasteurianus CICIM B7003, acetificationrate showed positive correlation with the activity of theenzymes in alcohol respiratory chain. Aiming at achievinghigh strength fermentation process, a series of trials weredesigned to raise the activity of AAB alcohol respiratorychain. Finally, acetification was enhanced by adding someprecursors (ferrous ions and β-hydroxybenzoic acid) ofalcohol respiration associated factors and increasing aerationrate (0.14 vvm). As final result, average acetification ratehas been raised to 2.29 ± 0.02 g/L/h, which was 28.7%higher than the original level. Simultaneously, it was foundthat the oxidization of alcohol into acetic acid in AAB cellswas improved by well balancing of three factors: enzymeactivity in alcohol respiratory chain, precursor of ubiquinonebiosynthesis, and aeration rate.

Efficient LDPC-Based, Threaded Layered Space-Time-Frequency System with Iterative Receiver

Junfeng Hu,Hailin Zhang,Yuan Yang 한국전자통신연구원 2008 ETRI Journal Vol.30 No.6

We present a low-density parity-check (LDPC)-based, threaded layered space-time-frequency system with emphasis on the iterative receiver design. First, the unbiased minimum mean-squared-error iterative-tree-search (U-MMSE-ITS) detector, which is known to be one of the most efficient multi-input multi-output (MIMO) detectors available, is improved by augmentation of the partial-length paths and by the addition of one-bit complement sequences. Compared with the U-MMSE-ITS detector, the improved detector provides better detection performance with lower complexity. Furthermore, the improved detector is robust to arbitrary MIMO channels and to any antenna configurations. Second, based on the structure of the iterative receiver, we present a low-complexity belief-propagation (BP) decoding algorithm for LDPC-codes. This BP decoder not only has low computing complexity but also converges very fast (5 iterations is sufficient). With the efficient receiver employing the improved detector and the low-complexity BP decoder, the proposed system is a promising solution to high-data-rate transmission over selective-fading channels.

Experimental and Numerical Investigation of Axial Tensile Strength of CHS X-Joints Reinforced with External Stiffening Rings

Lei Zhao,Lei Zhu,Hailin Sun,Lu Yang,Xi Chen 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.3

To study the reinforcement eff ect of the external stiff ening ring reinforced and unreinforced X-joints having a circular hollow section subjected to brace tensile load, an experimental and computer-simulated research has been done. Six full-scale X-joints are examined in this study: three reinforced specimens and three corresponding unreinforced ones. The test specimens cover diff erent brace to chord diameters ratios ( β ) of 0.25, 0.51, and 0.73. The experimental facilities and detailed parameters are presented. The experimental results including failure modes, load–displacement curves, and ultimate capacity are analyzed and compared. Two failure modes, weld failure and chord plastifi cation, are observed in the tests. The test results reveal an ultimate load enhancement in the reinforced joints by approximately 50% in comparison with the unreinforced joints and an initial stiff ness increase of 248.1% for the β of 0.25 and approximately 49–76% for the β of 0.51 and 0.73. A fi nite element model using shell elements is developed, which accurately predicts the static performance of the X-joints subjected to brace tensile load with and without external stiff ening rings.

Competitive Resource Sharing Based on Game Theory in Cooperative Relay Networks

Guopeng Zhang,Li Cong,Liqiang Zhao,Hailin Zhang,Kun Yang 한국전자통신연구원 2009 ETRI Journal Vol.31 No.1

This letter considers the problem of resource sharing among a relay and multiple user nodes in cooperative transmission networks. We formulate this problem as a sellers’ market competition and use a noncooperative game to jointly consider the benefits of the relay and the users. We also develop a distributed algorithm to search the Nash equilibrium, the solution of the game. The convergence of the proposed algorithm is analyzed. Simulation results demonstrate that the proposed game can stimulate cooperative diversity among the selfish user nodes and coordinate resource allocation among the user nodes effectively.