Comprehensive Assessment of Associations between ERCC2 Lys751Gln/Asp312Asn Polymorphisms and Risk of Non-Hodgkin Lymphoma

Zhou, Jue-Yu,He, Li-Wen,Liu, Jie,Yu, Hai-Lang,Wei, Min,Ma, Wen-Li,Shi, Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Excision repair crossing-complementing group 2 (ERCC2), also called xeroderma pigmentosum complementary group D (XPD), plays a crucial role in the nucleotide excision repair (NER) pathway. Previous epidemiological studies have reported associations between ERCC2 polymorphisms and non-Hodgkin lymphoma (NHL) risk, but the results have remained controversial. Materials and Methods: We conducted this meta-analysis based on eligible case-control studies to investigate the role of two ERCC2 polymorphisms (Lys751Gln and Asp312Asn) in determining susceptibility to NHL. Ten case-control studies from several electronic databases were included in our study up to August 14, 2014. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using fixed- or random-effects models to estimate the association strength. Results: The combined results based on all studies did not show any association between Lys751Gln/Asp312Asn polymorphisms and NHL risk for all genetic models. Stratified analyses by histological subtype and ethnicity did not indicate any significant association between Lys751Gln polymorphism and NHL risk. However, a significant reduced risk of NHL was found among population-based studies (Lys/Gln versus Lys/Lys: OR=0.87, 95% CI=0.77-0.99, P=0.037) but not hospital-based studies. As for Asp312Asn polymorphism, there was no evidence for the association between this polymorphism and the risk of NHL in all subgroup analyses. Conclusions: This meta-analysis suggests that there may be no association between Lys751Gln/Asp312Asn polymorphism and the risk of NHL and its two subtypes, whereas ERCC2 Lys751Gln heterozygote genotype may provide protective effects against the risk of NHL in population-based studies. Therefore, large-scale and well-designed studies are needed to clarify the effects of haplotypes, gene-gene, and gene-environment interactions on these polymorphisms and the risk of NHL and its different histological subtypes in an ethnicity specific population.

Effect of Microstructure on Mechanical Property of Carbon Steel/Strainless Steel Rolling Clad Plate

Li Hai-bin,Zhou Cun-long,Fan Xiao-ning,Kong Yi-gang,Huang Qing-xue,Ma Qin 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.11

The bonding interface microstructure and mechanical property of clad plate with different hot rolling schedules were researched. The results show that the carbide layer thickness of bonding interface increases firstly and then decreases with increasing reduction ratio. The carbide layer of bonding interface is the major factor relating to tensile strength, so the experimental values of clad plate are slightly higher than the calculated ones. Moreover, the bending strength of clad plate is mainly influenced by the thickness ratio and the carbide quantity of SS, while the carbide layer thickness of bonding interface is an important factor affects the bending strength.

A κ-Carrageenase from a Newly Isolated Pseudoalteromonas-like Bacterium, WZUC10

Mao-hong Zhou,Jian-she Ma,Jun Li,Hai-ren Ye,Ke-xin Huang,Xiao-wei Zhao 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5

A bacterial strain able to produce k-carrageenase, designated WZUC10, was isolated from a live specimen of the red alga Plocamium telfairiae collected in the East China Sea. The phylogenetic evidence and phenotypic features indicate that this strain belongs to the genus Pseudoalteromonas. WZUC10 requires NaCl for growth and k-carrageenan to induce k-carrageenase synthesis; galactose and lactose do not induce it. The optimal growth temperature is 23~27°C. The secreted enzyme, which has a molecular mass of 45 kDa, breaks down k-carrageenan into k-neocarratetraose sulfate and larger oli-gosaccharides with a repeating β-D-Galp4S-(1→4)-α-D-AnGalp structure, but cannot degrade k-neocarratetraose sulfate or k-neocarrahexaose sulfate into k-neocarrabiose sulfate. The enzyme retains 90% of its activity after 2 h at 40°C and is com-pletely inactivated after 7.5 min at 70°C. The enzyme’s optimal temperature is 30°C and its optimal pH is 7.5. The enzyme-catalyzed reaction follows Michaelis-Menten kinetics, with the Michaelis constant (Km) and the turnover number (k) being 0.015 mM and 125 s-¹, respectively. WZUC10 produces 50 U/mL k-carrageenase after cultivation at 25°C for 35 h on a me-dium containing 80 g/L glucose, 5 g/L corn steep liquor, 3 g/L k-carrageenan, and 15 g/L NaCl. k-Neocarratetraose sulfate was prepared simply with precipitation by ethanol:water (5:1, v/v).

Analysis of microRNA expression profiles during the cell cycle in synchronized HeLa cells

( Jue Yu Zhou ),( Wen Li Ma ),( Shuang Liang ),( Ye Zeng ),( Rong Shi ),( Hai Lang Yu ),( Wei Wei Xiao ),( Wen Ling Zheng ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.9

Oral Session : Symposium: Engineering and Technology for Human and Ecosystem Health ; Use of Remote Sensing Technologies and 137Cs Measurements to Estimate Soil Erosion Quantity in Semi-arid Steppe Ar

Zhan Jiang Sha,Hai Zhou Ma,Ling Qin Li,Qi Shun Fan 한국토양비료학회 2009 국제학술대회집 Vol.2009 No.0

A novel M2e-multiple antigenic peptide providing heterologous protection in mice

Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.

Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation

Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Guangzhi Tong 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.3

Novel reassortant H3N2 swine influenza viruses (SwIV)with the matrix gene from the 2009 H1N1 pandemic virushave been isolated in many countries as well as duringoutbreaks in multiple states in the United States, indicatingthat H3N2 SwIV might be a potential threat to public health. Since southern China is the world’s largest producer of pigs,efficient vaccines should be developed to prevent pigs fromacquiring H3N2 subtype SwIV infections, and thus limit thepossibility of SwIV infection at agricultural fairs. In thisstudy, a high-growth reassortant virus (GD/PR8) wasgenerated by plasmid-based reverse genetics and tested as acandidate inactivated vaccine. The protective efficacy of thisvaccine was evaluated in mice by challenging them withanother H3N2 SwIV isolate [A/Swine/Heilongjiang/1/05(H3N2) (HLJ/05)]. Prime and booster inoculation withGD/PR8 vaccine yielded high-titer serum hemagglutinationinhibiting antibodies and IgG antibodies. Completeprotection of mice against H3N2 SwIV was observed, withsignificantly reduced lung lesion and viral loads invaccine-inoculated mice relative to mock-vaccinatedcontrols. These results suggest that the GD/PR8 vaccine mayserve as a promising candidate for rapid intervention ofH3N2 SwIV outbreaks in China.

Efficacy of Pap Test in Combination with ThinPrep Cytological Test in Screening for Cervical Cancer

Chen, Hua,Shu, Hui-Min,Chang, Zhou-Lin,Wang, Zhi-Feng,Yao, Hai-Hong,Zhu, Hong-Mei,Lu, Tian-Mei,Ma, Qiang-Yan,Yang, Bin-Lie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background: Our aim was to investigate the efficacy of the Pap test in combination with the ThinPrep cytological test (TCT) in screening for cervical cancer in China. Design: From March 2006 to October 2008, 988 women with the mean age $46.4{\pm}10.5$ years (range, 23-80 years) were recruited to receive cervical cancer screening. Pap test results ${\geq}$ grade III and TCT findings ${\geq}$ ASCUS/AGUS were considered abnormal. Subjects with a Pap test result ${\geq}$ grade IIb received TCT. Colposcopy and biopsies were performed in all participants, and final diagnosis was based on pathological findings. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and Youden index for predicting CIN I or above were determined. Results: The sensitivity, specificity, PPV, NPV and Youden index of the Pap test were 43.1%, 97.2%, 70.0%, 91.9%, and 40.3%, respectively. The same values for TCT in predicting CIN were 80.0%, 63.2%, 16.0%, 97.3%, and 43.2%, respectively. The two tests in combination gave values for predicting CIN of 64.8%, 87.6%, 43.6%, 94.4%, and 53.5%, respectively. Combined testing exhibited the highest Youden index (53.4%). Conclusion: The Pap test with a reduced threshold in combination with the TCT has high sensitivity and high specificity in screening for cervical cancer.

Parameter Sensitivity Analysis of a New Fabricated Rectangular Tunnel Joint Using Numerical Method

Zhen Huang,Chenlong Zhang,Shaokun Ma,Hai Zhang,Zhang Zhou,Hongzhou Li 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.2

An assembled rectangular tunnel has the advantages of high space utilization rate and strong urban applicability. This solution has been widely used in urban underground traffic construction. During their construction and operation, the joints are the weakest parts of the structure. The mechanical behavior of joints is closely related to the safety of the tunnel; thus, it is important to use a joint with good mechanical behavior. This study presents a new type of joints for shallow buried assembled rectangular tunnels. To evaluate the effect of design parameters (tenon depth and tenon angle) on the behavior of joints, the finite element method was used. At the same time, the mechanical behavior of rectangular tunnel joint was analyzed and compared with that of a traditional straight joint under the same loading conditions, showing better results. The performance-based engineering (PBE) concept was implemented to evaluate the robustness of rectangular tunnel joints. The results show that if the bolt is placed on the tensile side of the structure, it provides a strong restraining effect on the deformation of the joint. When the tenon depth and angle are 0.8 m and 3.6°, respectively, the overall behavior of the rectangular tunnel joint was improved. The new type of assembled rectangular tunnel joint designed in this study has a good application prospect in a shallow stratum and provides new ideas for the design and construction of such tunnels.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.