Lamotrigine Decreased Hippocampal Damage and Improved Vascular Risk Markers in a Rat Model of Pentylenetetrazole Induced Kindling Seizure

Haggag, Basma S.,Hasanin, Amany H.,Raafat, Mona H.,Kawy, Hala S. Abdel The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3

Various antiepileptic drugs (AEDs) especially enzyme-inducing AEDs might be associated with increased vascular risk, through impairment of the endogenous antioxidative ability which may trigger oxygen-dependent tissue injury. Lamotrigine (LTG) a non-enzyme-inducing AED has scarce information regarding its effects on oxidative stress. The present study aimed to study the possible modulation of vascular risk factors of epileptogenesis by LTG, in a rat model of kindling seizure induced by pentylenetetrazole (PTZ). Four groups of male Wister rats were used; vehicle control group, PTZ group (alternate day PTZ, 30 mg/kg, i.p), LTG/PTZ group (LTG 20 mg/kg/day p.o and alternate day PTZ) and LTG group. The study period was 5 weeks. Lipoproteins and total homocysteine (tHcy), malondialdehyde (MDA) and reduced glutathione (GSH) were measured. Aortic endothelial function study and histopathological examination of the rats' brains, aortas and coronaries were conducted. Serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), tHcy, MDA, GSH levels were significantly higher in epileptic rats than normal controls rats. A decrease in HDL-cholesterol with high atherosclerotic index was also demonstrated. The administration of LTG improved the PTZ-kindled seizures. It produced a significant decrease in TC, TG and LDL-cholesterol, MDA, aortic GSH and increase in HDL-cholesterol with no significant effect on serum GSH and tHcy levels. LTG improved endothelium-dependent relaxation, decreased hippocampal neurodegenerative changes and atherosclerotic changes of aortas and coronaries. LTG decreased seizures severity, hippocampal damage and improved vascular risk markers in this rat model of kindling seizures.

Comparative evaluation of the ultrastructural morphology and distribution of filiform and fungiform tongue papillae in Egyptian mice, fruit bats and long-eared hedgehogs

Tahany Haggag,Elham F. Mahmoud,Zeinab A. Salem,Nermeen AbuBakr 대한해부학회 2020 Anatomy & Cell Biology Vol.53 No.4

The tongue is a specialized vital organ. It aids in mastication, deglutition and food digestion. It also shares in the perception of taste sensation as it possesses various gustatory papillae. It is being subjected to numerous anatomical and histological examinations aiming at exploring the correlation between its morphological features and animal adaptations to various types of nutrition and environmental conditions. The goal of the present work was to compare the ultrastructural features of the filiform and fungiform papillae of three various mammals possessing different feeding habits; Egyptian mice, fruit bats and long-eared hedgehogs. Specimens were obtained from the tongues of four healthy adult animals from each mammalian type. Tongues were fixed and all the appropriate procedures were done to perform scanning electron microscopic investigation. Scanning electron microscopic examination demonstrated that in mice, there were four different sub-types of filiform papillae (spike, leaf, conical and tongue-shaped). In bats, there were two sub-types (flower and leaf-like) and in hedgehogs, there was only one type (tongue-like). These filiform papillae showed different distribution and orientation. As for the fungiform papillae, they were cylindrical in mice, rounded or conical in bats and dome-shaped in hedgehogs. Fungiform papillae possessed taste pores containing taste buds. Ultrastructural variations of the filiform and fungiform papillae were suggested to be probably due to adaptation to various feeding habits and different environmental conditions of these animals.

Lamotrigine Decreased Hippocampal Damage and Improved Vascular Risk Markers in a Rat Model of Pentylenetetrazole Induced Kindling Seizure

Basma S Haggag,Amany H Hasanin,Mona H Raafat,Hala S Abdel Kawy 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3

Various antiepileptic drugs (AEDs) especially enzyme-inducing AEDs might be associated withincreased vascular risk, through impairment of the endogenous antioxidative ability which may triggeroxygen-dependent tissue injury. Lamotrigine (LTG) a non-enzyme-inducing AED has scarce informationregarding its effects on oxidative stress. The present study aimed to study the possible modulationof vascular risk factors of epileptogenesis by LTG, in a rat model of kindling seizure induced bypentylenetetrazole (PTZ). Four groups of male Wister rats were used; vehicle control group, PTZ group(alternate day PTZ, 30 mg/kg, i.p), LTG/PTZ group (LTG 20 mg/kg/day p.o and alternate day PTZ)and LTG group. The study period was 5 weeks. Lipoproteins and total homocysteine (tHcy), malondialdehyde(MDA) and reduced glutathione (GSH) were measured. Aortic endothelial function studyand histopathological examination of the rats’ brains, aortas and coronaries were conducted. Serumtotal cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), tHcy, MDA,GSH levels were significantly higher in epileptic rats than normal controls rats. A decrease inHDL-cholesterol with high atherosclerotic index was also demonstrated. The administration of LTGimproved the PTZ-kindled seizures. It produced a significant decrease in TC, TG and LDL-cholesterol,MDA, aortic GSH and increase in HDL-cholesterol with no significant effect on serum GSH and tHcylevels. LTG improved endothelium-dependent relaxation, decreased hippocampal neurodegenerativechanges and atherosclerotic changes of aortas and coronaries. LTG decreased seizures severity,hippocampal damage and improved vascular risk markers in this rat model of kindling seizures.

Evaluation of Comfort Attributes of Polyester Knitted Fabrics Treated with Sericin

R. F. El-Newashy,S. Mowafi,K. Haggag,M. Abou Taleb,H. El-Sayed 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9

This work was adopted to study the effect of sericin-containing bath on the different structures of knitted polyesterfabrics to improve some of their comfort attributes. Without appreciable deterioration in its inherent properties, the differentstructures of treated polyester fabric exhibited induced hydrophilicity, enhanced electrical conductivity, improved resistanceto ultraviolet radiation and better water vapour permeability. Five knitted polyester fabrics of different structures weresaponified by sodium hydroxide to create carboxylate ions along their macromolecules, and then treated by sericin withinfreshly discharged bath from degumming of raw natural silk. Treatment of polyester fabrics with sericin was carried out inpresence and absence of glutardialdehyde as a crosslinking agent between polyester and sericin macromolecules. Differentthermo-physiological parameters of comfort; namely thermal conductivity/resistivity, air/water permeability, release ofelectrostatic charges, ultraviolet protection factor, and water vapour transmission of the sericin-treated as well as untreatedknitted fabrics were assessed. The mechanism of interaction between knitted polyester fabric and sericin in presence ofglutardialdehyde was proposed. Scanning electron microscopy was used to ascribe any alteration in the fibre morphology. The change in the fabric crystallinity was investigated using X-ray diffraction pattern. The burst strength, bending stiffnessand yellowing index of the treated fabrics were in the acceptable range.

Combination between Taxol-Encapsulated Liposomes and Eruca sativa Seed Extract Suppresses Mammary Tumors in Female Rats Induced by 7,12 Dimethylbenz(α)anthracene

Shaban, Nadia,Abdel-Rahman, Salah,Haggag, Amany,Awad, Doaa,Bassiouny, Ahmad,Talaat, Iman Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.1

Taxol (paclitaxel) is a powerful anti-cancer drug widely used against several types of malignant tumors. Because Taxol may exert several side effects, a variety of formulations have been developed. One of these features liposomes, regarded as one of the most promising drug carriers, biocompatible and best able to reduce drug toxicity without changing efficacy against tumor cells. Eruca sativa seed extract (SE) is considered a promising natural product from cruciferous vegetables against breast cancer, increasing chemotherapeutic and eliminating harmful side effects. The effects of Taxol-encapsulated liposomes (T) alone and in combination between Eruca sativa seed extract on nuclear factor kappa B (NF-${\kappa}B$), cyclooxygenase-2 (COX-2) and B-cell lymphoma-2 (Bcl-2) gene expression levels were investigated in rat mammary gland carcinogenesis induced by 7,12 dimethylbenz(${\alpha}$) anthracene (DMBA) using qRT-PCR. The results showed that DMBA increased NF-${\kappa}B$, COX-2 and Bcl-2 gene expression levels and lipid peroxidation (LP), while decreasing glutathione-S-transferase (GST) and superoxide dismutase (SOD) activities and total antioxidant concentration (TAC) compared to the control group. T and T-SE treatment reduced NF-${\kappa}B$, COX-2 and Bcl-2 gene expression levels and LP. Hence, T and T-SE treatment appeared to reduce inflammation and cell proliferation, while increasing apoptosis, GST and SOD activities and TAC.

Inhibition of NF-ĸB, Bcl-2 and COX-2 Gene Expression by an Extract of Eruca sativa Seeds during Rat Mammary Gland Carcinogenesis

Abdel-Rahman, Salah,Shaban, Nadia,Haggag, Amany,Awad, Doaa,Bassiouny, Ahmad,Talaat, Iman Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

The effect of Eruca sativa seed extract (SE) on nuclear factor kappa B (NF-${\kappa}B$), cyclooxygenase-2 (COX-2) and B-cell lymphoma-2 (Bcl-2) gene expression levels was investigated in rat mammary gland carcinogenesis induced by 7,12 dimethylbenz(${\alpha}$)anthracene (DMBA). DMBA increased NF-${\kappa}B$, COX-2 and Bcl-2 gene expression levels and lipid peroxidation (LP), while, decreased glutathione-S-transferase (GST) and superoxide dismutase (SOD) activities and total antioxidant concentration (TAC) compared to the control group. After DMBA administration, SE treatment reduced NF-${\kappa}B$, COX-2 and Bcl-2 gene expression levels and LP. Hence, SE treatment reduced inflammation and cell proliferation, while increasing apoptosis, GST and SOD activities and TAC. Analysis revealed that SE has high concentrations of total flavonoids, triterpenoids, alkaloids and polyphenolic compounds such as gallic, chlorogenic, caffeic, 3,4-dicaffeoyl quinic, 3,5-dicaffeoyl quinic, tannic, cinnamic acids, catechin and phloridzin. These findings indicate that SE may be considered a promising natural product from cruciferous vegetables against breast cancer, especially given its high antioxidant properties.

An Investigation of the Deformation Mechanisms in Sn5% Sb Alloy Using Tensile , Creep and ABI Tests from Ambient to 473K

Murty, K . L .,Mathew, M . D .,Haggag, F . M . 대한금속재료학회(대한금속학회) 1998 METALS AND MATERIALS International Vol.4 No.4

Tensile, creep, and automated ball indentation (ABI) tests have been conducted to study deformation mechanisms in Sn5%Sb alloy between ambient and 473 K. A power law relationship was obtained between minimum creep rate and applied stress, with stress exponent, n=5 and activation energy, Q=12.6±1.1 kCal/mole. At 473 K, a transition from n=5 to n=3 was observed at low stresses. ABI tests showed a power law relationship between strain rate and ultimate tensile stress with values of n=5 and Q=13.0±1.8 kcal/mole. Tensile results were in broad agreement with the creep and ABI data. A new deformation mechanism is proposed for the n=5 region involving viscous glide of dislacations assisted by dislocatian care diffusion.

Synthesis and Structure of Purine Derivatives as Antitumor Effects

Moharram, H.H.,El-Bayouki, Khairy A.M.,Haggag, B.,Basyouni, W.M.,Osman, A.M. The Pharmaceutical Society of Korea 1989 Archives of Pharmacal Research Vol.12 No.2

The nucleophilic substitution reaction of 6-chloro purines (I) with malononitrile and ethyl cyanoacetate is carried out in DMSO and in the presence of an alkali. The possible tautomeric-ylidene form for the products is considered and discussed in view of IR, UV, NMR and mass spectral determinations. The derivatives were tested for their antitumor activities.

Hemodynamic effects of norepinephrine versus phenylephrine infusion for prophylaxis against spinal anesthesia-induced hypotension in the elderly population undergoing hip fracture surgery: a randomized controlled trial

Mostafa Maha,Hasanin Ahmed,Mostafa Mahmoud,Taha Mai Y,Elsayad Mohamed,Haggag Fatma Alzahraa,Taalab Omar,Rady Ashraf,Abdelhamid Bassant 대한마취통증의학회 2021 Korean Journal of Anesthesiology Vol.74 No.4

Background: Elderly population are at increased risk of spinal anesthesia-induced hypotension increasing their risk for postoperative morbidity and mortality. This study aimed to compare the hemodynamic effects of prophylactic infusion of norepinephrine (NE) versus phenylephrine (PE) in elderly patients undergoing hip fracture surgery under spinal anesthesia. Methods: Elderly patients scheduled for hip fracture surgery were randomized to receive either NE infusion (8 µg/min) (NE group, n = 31) or PE infusion (100 µg/min) (PE group, n = 31) after spinal anesthesia. Outcomes included mean heart rate, mean blood pressure, cardiac output, incidence of spinal anesthesia-induced hypotension, incidence of bradycardia, and incidence of hypertension. Results: Sixty-two patients with a mean age of 71 ± 6 years were included in the final analysis (31 patients in each group). The NE group showed a higher mean heart rate and cardiac output than the PE group. The NE group had a lower incidence of reactive bradycardia (10% vs. 36%, P = 0.03) and hypertension (3% vs. 36%, P = 0.003) than the PE group. No study participant developed hypotension, and the mean blood pressure was comparable between the two groups. Conclusions: Both NE and PE infusions effectively prevented spinal anesthesia-induced hypotension in elderly patients undergoing hip fracture surgery. However, NE provided more hemodynamic stability than PE; maintaining the heart rate, higher cardiac output, less reactive bradycardia, and hypertension.

Imparting Durable Ultraviolet Protection to Polyamide 6 Fabric

H. El-Sayed,A. Abou El-Kheir,L. K. El-Gabry,K. Haggag 한국섬유공학회 2019 Fibers and polymers Vol.20 No.10

Deterioration of polyamide 6 fabrics upon exposure to ultraviolet radiation for a long time is an acute dilemma. Imparting ultraviolet protection to polyamide fabrics is of prime importance from the performance and economic point ofviews. In this investigation, polyamide 6 fabrics were modified with an environmentally friendly product (sericin) in presenceor absence of crosslinking agents; namely citric acid or epichlorohydrin using pad-dry-cure technique. Topographicalinvestigation of the used sericin powder was conducted using transmission electron microscopy (TEM). The morphologicalstructure of treated as well as untreated fabrics was investigated using high resolution field emission scanning electronmicroscope (FE-SEM). The alteration in some physico-mechanical characteristics of the treated polyamide 6 fabrics; Viz. bending stiffness, burst test, degree of yellowness, and moisture regain were assessed. The treated polyamide fabrics weredyed with acid and reactive dyestuffs. The colour strength and washing fastness for dyed fabrics with both acid and reactivedyes were assessed. The photo-protection against UV light of the said fabrics were evaluated. The treated fabric exhibitedexcellent UV protection without significant discrepancy in their inherent physico-mechanical properties.