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- 저자 : Hafiz U. Rehman (검색결과 2 건)
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Tayyaba Mateen,Kazuaki Okamoto,Sun-Lin Chung,Kuo-Lung Wang,Hao-Yang Lee,Shuhei Abe,Yuji Mita,Hafiz U. Rehman,Masaru Terabayashi,Hiroshi Yamamoto 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.6
U-Pb-Hf isotopes of zircons from the granitic intrusive rocks in the Iwakuni area of southwest Japan are determined by LA-ICP-MS analysis. The Gamano Granodiorite belonging to the Older Ryoke Granites of the Ryoke Belt yielded U-Pb mean age of 92.3 ± 2.6 Ma (MSWD = 1.8). The Namera Granite belonging to the Younger Ryoke Granites of the Ryoke Belt yielded U-Pb mean age of 106.3 ± 1.6 Ma (MSWD = 2.3). The Shimokuhara Granite belonging to the Hiroshima Granites of the San-yo Belt yielded U-Pb mean age of 103.8 ± 1.5 Ma (MSWD = 0.32). A granite porphyry sample taken from about 15 m thick dike discordantly intruded into pelitic schist of the Ryoke metamorphic rocks yielded U-Pb mean age of 92.5 ± 1.6 Ma (MSWD = 0.63). Hf isotope data, obtained from the same dated zircon grains, show initial 176Hf/177Hf isotopic ratios referred to as “εHf(t)” generally ranging between –4.8 and +1.1 except an anomalously low value (–15.0) from an inherited core. The εHf(t) values, calculated with average crustal 176Lu/177Hf ratio of 0.015, correspond to Hf isotope crustal model ages between 1474 to 1087 Ma (Mesoproterozoic). The Gamano Graodiorite has long been regarded to be a syn-tectonic intrusion, but the zircon ages and εHf(t) values are similar to those of the post-tectonic granite porphyry dike. The results of this study together with available U-Pb zircon ages of granitic rocks in the Iwakuni area reported elsewhere reveal that the order of emplacement indicated by the U-Pb zircon ages are much discrepant with the traditional classification, the Older Ryoke, the Younger Ryoke. It is better to refrain from the use of “Older” and “Younger” for the collective names of the granitic intrusive bodies.
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Molecular detection and characterization of ovine herpesvirus-2 using heminested PCR in Pakistan
Aayesha Riaz,Inga Dry,Robert Dalziel,S. U. Rehman,Muhammad Ali Shah,Hafiz Muhammad Naeem Akhtar,Arfan Yousaf,Ruqia Baig 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4
Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Objectives: This study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods. Methods: Blood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. The samples were subjected to heminested polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the OvHV-2 POL gene and the OvHV-2 ORF75 tegument protein gene. Results: The highest percentage of MCF positive samples was in sheep (13%), whereas goat, cattle, and buffalo had lower positive percentages, 11%, 9%, and 6.5%, respectively. Four OvHV-2-positive PCR products obtained from sheep samples were sequenced. The sequences obtained were submitted to the NCBI GenBank database (MK852173 for the POL gene; MK840962, MK852171, and MK852172 for the ORF75 tegument protein gene). Phylogenetic analysis revealed a close similarity of study sequences with those of worldwide samples. Conclusions: This study is the first cross-sectional study on the prevalence and molecular detection of OvHV-2 in apparently healthy cattle and buffalo that could be carrying OvHV-2 acquired from OvHV-2-positive sheep and goats. The results indicate that OvHV-2 is circulating in Pakistan. Further studies are needed to characterize OvHV-2 and elucidate further its prevalence.
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