당의 종류 및 첨가량을 달리한 깨다식의 품질특성

김진숙,한영실,유선미,김행란,전혜경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.3

깨다식의 감미 및 물성 등의 개선을 위해 꿀보다 높은 점도와 hardness를 가지는 쌀엿과 조청 등을 첨가 혼합하여 품질을 조사한 결과를 요약하면 다음과 같다. 깨다식의 적정 재료 배합비는 깨가루 100 기준시, 꿀과 쌀엿의 동량 혼합액 50%(w/w)였으며, 이를 기준으로 제조한 깨다식의 이화학적 특성은 당도 17.44 brix˚, tnqns 12.16, 환원당 15.44, 단백질 12.88, 지방 32.02, 조회분 2.82 %이었고, 기계적 특성 중 TPA의 chewiness는 0.28~0.30, hardness는 27.22~27.44 g/㎠이고, 색도의 L값은 41.67, a값 4.58, 그리고 b값은 16.99였다. This study was carried out to investigate the effects of the addition of sweeteners to Sesame Dasiks. Sesame Dasiks were prepared by adding various sweeteners to sesame seeds powder, at 40, 50, 60 and 70%, and their quality characteristics were measured. In the chewiness and hardness analyses of Sesame Dasiks, the 50% group (the same ratio and mixture of honey and lire jelIy) gave the highest results. For the sweetness, taste and overall acceptability of the Sesame Dasiks, the 50% group was also the better The texture profile analyses found the optimal chewiness and hardness of the Sesame Dasik to be 0.28 ~ 0.30 and 27.22 ~ 27.44, respectively.

재래종 콩 유전자원의 주요 형태적 특성과 변이

윤문섭,백형진,이정란,김행훈,조양희,안종웅,김창영 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

본 실험은 한국, 중국 및 일본 재래종 콩 유전자 자원의 주요형질 변이를 조사하고 이들 자원간의 지리적 유연관계를 비교하고자 수행하였으며, 그 결과를 요약하면 다음과 같다. 1. 경장은 한국(66㎝)이나 일본자원(54.9㎝)보다 중국자원(71㎝)에서 더 컸으며, 또한 변이도 중국자원에서 가장 큰 것으로 나타났다. 2. 개화일수 및 생육일수는 한국자원(65.5일, 151.4일)이 가장 길었으며 중국(57일, 120일)과 일본자원(59.9일, 122.4일)은 같은 경향을 나타냈다. 또한 한국과 중국자원은 지역에 따른 차이를 나타냈으나 일본자원에서는 확인할 수 없었다. 3. 백립중은 한국(18.8g), 일본(17.2g) 및 중국(14.7g) 순으로 무거웠다. 특히 한국자원은 9~11g과 23~25g에서 정점을 나타냈고, 변이폭도 가장 컸다. 반면 일본과 중국자원은 13~17g사이에서 정점을 나타냈다. 4. 협수는 중국(46.9개)이나 일본자원(36.5개)보다 한국자원(75.5개)에서 가장 많았으며, 변이폭도 한국자원에서 가장 컸다. 내도복성은 일본자원에서 가장 강하였다. 5. 정준판별분석결과 can 1을 경계로 하여 오른쪽 상하에 한국재래종이 주로 분포하였고 왼쪽에는 중국과 일본 재래종이 주로 분포하여 각 나라별로 고유한 영역을 가지고 분리되었다. 전체적 경향이 중국과 일본자원이 한국자원보다 가까운 경향을 나타냈다. This experiment was carried out to compare the characters of 944 Korean soybean landraces, 716 Chinese, and 170 Japanese soybean landraces introduced from USDA soybean germplasm collection. Cannonical discriminant and cluster analyses were conducted by their origins. The population from China was taller in plant height than those from Korea and Japan, also its variation was largest in Chinese population. The population from Korea was langer than those from China and Japan in terms of days to flowering and maturity. Also, Korean and Chinese accessions appeared the difference of those traits according to latitude. One hundred seeds weight was in the order of Korean(18.8g), Japanese(17.2g) and Chinese populations(14.7g), especially Korean population was distinguished into two groups; the group including accessions between 10 and 15g, and the other group including accessions between 20 and 25g. However, others showed the peak in range of 13~17g. The number of pod per plant was more in Korean accession(75.5) with the largest variation than Chinese(46.9) and Japanese accession(36.5). The distribution of Korean, Chinese and Japanese accessions reflected the origin of accessions analyzed by cannonical discriminant analysis. The relationship between their geographical origins showed the population of China and Japan are losely related.

국산·수입산 동부를 이용한 모시잎송편의 영양성분비교

김영섭,김소민,오지연,황진봉,이기택,신정아,김상천,김세나,김행란,황영,최용민 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Anti-Helicobacter pylori Diarylheptanoid Identified in the Rhizome of Alpinia officinarum

( Haeng Byung Lee ),( Hyun Kyung Lee ),( Jun Ran Kim ),( Young Joon Ahn ) 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.4

Poster Session : Cancer Biology ; Phosphory lation of c-Myc on Ser62 by CDK5 is essential for cyclin G1-mediated transcriptional activation of cyclin B1

( Haeng Ran Seo ),( Joon Kim ),( Shang Woo Bae ),( Jae Won Soh ),( Yun Sil Lee ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Anti-Helicobacter pylori Diarylheptanoid Identified in the Rhizome of Alpinia officinarum

( Haeng Byung Lee ),( Hyun Kyung Lee ),( Jun Ran Kim ),( Young Joon Ahn ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.4

The antibacterial activity of materials derived from the rhizome of Alpinia officinarum (Zingiberaceae) against Helicobacter pylori ATCC 43504, ATCC 700392, and ATCC 700824 was examined using paper-disc diffusion and agar dilution methods. Results were compared with those following treatment with currently used antibiotics: amoxicillin, metronidazole, and tetracycline. The bioactive principle was characterized as the diarylheptanoid 5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone by spectroscopic analysis. This compound was isolated from A. officinarum leaves as a new anti-H. pylori principle. Against H. pylon ATCC 43504, ATCC 700392, and ATCC 700824, the antibacterial activity of the diarylheptanoid (48,24, and 24 ㎍/mL) was comparable to that of metronidazole (32, 16, and 16 ㎍/mL) but less effective than that of either amoxicillin (0.06, 0.06, and 0.03 ㎍/mL) or tetracycline (0.5, 1, and 0.5 ㎍/mL), based on minimum inhibitory concentrations. A. officinarum rhizome-derived materials, particularly the diarylheptanoid isolated, merit further study as potential antibacterial functional food products or therapeutic products for prevention or eradication from humans from diseases caused by H. pylori.

강정 제조시 찹쌀의 수침조건에 따른 찹쌀과 수침액의 미생물상, 효소활성 및 미세구조 변화

김행란(Haeng Ran Kim),김경미(Kyung Mi Kim),김광옥(Kwang Ok Kim) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.6

수침조건에 따라 수침찹쌀과 수침액의 미생물학적 변화와 효소활성 및 구조적 특성을 살펴본 결과 수침액의 미생물상에서, 수침초기에는 젖산균이 우세하였고, 수침기간이 길어짐에 따라 미생물 균수가 급속히 증가하면서 젖산균과 Bacillus spp.이 우세균총으로 관찰되다가 수침이 계속되면서 모든 미생물이 감소하는 경향을 보였으며 그 중에서 Bacillus spp.이 가장 많이 관찰되었다. 수침액의 α-amylase는 수침온도와 수침기간이 증가함에 따라 지속적으로 활성이 증가하였고, protease 활성은 수침기간이 길어짐에 따라 활성이 증가한 후 감소하는 경향을 보였다. 수침 찹쌀의 α-amylase는 수침온도가 높을수록 감소하였고, β-amylase와 glucoamylase는 수침 1일에 증가하여 수침 21일까지 일정하였다. 수침찹쌀의 미세구조를 살펴보면, 세포벽이 떨어져 나간 흔적이 보이고 다각형의 찹쌀전분입자들이 노출되면서 표면이 거칠어 보였다. 또한 찹쌀전분 입자 표면의 미세한 구멍이 수침 11일 이후 관찰되어 효소작용의 결과와 일치하는 경향을 나타냈다. This study was conducted to investigate the effects of steeping periods and temperatures of waxy rice on the microflora and enzyme activities of steeping water and waxy rice, as well as on the microscopic structures of waxy rice during the preparation of gangjung. When the steeping water was 15 and 35℃, yeast and lactic acid bacteria were the major microflora after 1 day. After 11 days, Lactobacillus spp. were the major bacteria, while Bacillus spp. were the primary microflora after 21 days. At 25℃ the primary species were Bacillus spp., Leuconostoc spp., and Bacillus spp. after 1, 11 and 21 days, respectively. The α-amylase activity of steeping water increased proportionally with the increase of temperature and period, while the protease activity tended to increase for 11 days, but decreased at 21 days. The enzyme activities of the steeped waxy rice, especially the α-amylase activity, decreased with higher steeping temperature, while the β-amylase and glucoamylase activity remained constant for up to 21 days. Observation using scanning electron microscopy indicated that intracellular cleavage of steeped waxy rice endosperm resulted in a rough surface morphology due to the exposure of polygonal starch granules. Tiny pinholes on the surface of waxy rice starch granules were also observed after steeping for 11 days.

이천 게걸무, 강화 순무, 조선무의 화학적 특성 및 효소활성

김행란(Haeng-Ran Kim),이지현(Ji-Hyun Lee),김양숙(Yang-Suk Kim),김경미(Kyung-Mi Kim) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.3

본 연구에서는 이천 게걸무, 강화 순무 및 조선무의 품질 특성을 비교 분석하였다. 일반성분의 경우, 수분 함량은 이천 게걸무 87.78%, 강화 순무 92.73% 및 조선무 91.45%로 게걸무가 다른 무에 비하여 낮았다. 반면에 조단백, 조섬유 및 조회분 함량은 이천 게걸무가 강화 순무와 조선무에 비하여 약 1.7-1.8배, 1.2-1.6배 및 2.0-2.4배 높았다. 식이섬유함량은 이천 게걸무 11.32%, 강화 순무 13.26% 및 조선무 14.20%로 게걸무가 다른 무에 비하여 낮았다. 무기질 함량의 경우, 게걸무의 Mg, K, Ca 함량이 다른 무에 비하여 높았으며 P, Zn, Fe 함량은 다른 무와 유의적인 차이가 없었다. 가수분해 효소로서 소화와 관련된 α-amylase 활성의 측정 결과 이천 게걸무 9.97 unit, 강화 순무 14.87 unit 및 조선무 18.99 unit으로 나타났다. Protease 활성의 경우, 알칼리성은 이천 게걸무와 조선무가 각각 3.02 PU, 3.10 PU의 유사한 수준으로 순무에 비하여 높았고, 산성 protease 활성에서는 게걸무가 가장 높았다. 무의 매운맛 성분 및 기능성 관련 물질로 알려진 myrosinase 활성은 이천 게걸무가 36,990.81 unit으로 강화 순무나 조선무보다 높았다. 결론적으로 이천 게걸무는 강화 순무나 조선무에 비하여 조단백, 회분, 조섬유 및 무기질 중 Na, Mg, K, Ca의 함량이 높고 protease 활성과 myrosinase 활성이 높았다. For this investigation, we analyzed the chemical characteristics and enzyme activities of Icheon Ge-Geol radish, Gangwha turnip, and Korean radish. Regarding their proximate compositions, the water contents of the Icheon Ge-Geol radish, Gangwha turnip, and Korean radish were 87.78, 92.73, and 91.45%, respectively. The crude protein, crude fiber, and ash contents of the Icheon Ge-Geol radish were 1.35, 1.11, and 1.55%, respectively, which were much higher than the contents of the other samples; however, total dietary fiber was significantly lower. For mineral content, the magnesium, potassium, and calcium levels of the Icheon Ge-Geol radish were higher than the other samples. With regards to the enzyme activities of the samples, the protease and myrosinase activities of the Icheon Ge-Geol radish were higher than in the other samples. For α-amylase activity, the Korean radish showed the strongest activity (18.99 units/g of sample), followed by the Gangwha turnip and Icheon Ge-Geol radish.

Radiation-induced cathepsin S is involved in radioresistance

Seo, Haeng Ran,Bae, Sangwoo,Lee, Yun-Sil Wiley Subscription Services, Inc., A Wiley Company 2009 International journal of cancer: Journal internati Vol.124 No.8

<P>Previous studies have suggested that the production of cathepsin S (CatS), a cysteine protease, was specifically induced in radiation-induced rat mammary tumors. In this study, we further investigate the mechanism by which CatS is induced by radiation and its function. Radiation induced production of CatS at both the mRNA and protein level, and increased its protease activity. In addition, these radiation induced changes occurred in a dose and time-dependent fashion. Agents such as bleomycin, As<SUB>2</SUB>O<SUB>3</SUB> and H<SUB>2</SUB>O<SUB>2</SUB>, which produce reactive oxygen species (ROS), also induced CatS expression; however, other agents that damage DNA such as taxol and cisplatin did not. Additionally, treatment of the cells with the ROS scavengers, N-acetylcysteine and catalase, inhibited the radiation induced CatS expression. Furthermore, radiation-induced ROS was also involved in IFN-γ production, which was responsible for radiation-mediated CatS expression. Moreover, electrophoretic mobility shift assay (EMSA) data obtained using an IFN-stimulated response element (ISRE) oligonucleotide revealed that IFN regulatory factor-1 (IRF1) was the critical transcriptional mediator of IFN-γ-dependent CatS production after radiation. Finally, CatS overespression was found to induce radioresistance; however, knockdown of CatS resulted in the suppression of radioresistance. Taken together, the results of this study indicate that radiation induced CatS expression via ROS-IFN-γ pathways, and that this increased expression may be involved in radioresistance. © 2008 Wiley-Liss, Inc.</P>