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T subset定量을 위한 抗牛赤血球 IgG抗體의 分離·精製
夏潤文,李振鏞,林壽德 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1981 東西醫學硏究所 論文集 Vol.1 No.1
사람에 있어서 免疫담당세포의 하나인 T 細胞는 몇몇 subpooulation으로 나누어지고 있으며, 그중 ?? 및 ??를 同定하는 수단으로 사용되는 牛赤血球抗體중에서 우선 純粹 IgG抗體를 分離, 精裂하였으며, 이 精裂된 IgG抗體는 標準製品과의 比較實驗에서 ?? 細胞의 一致되는 成積을 얻을 수 있었다. Antisera to ox red blood cell were prepared by intraperitioneal hypermultiple injections without adjuvant in outbred white rabits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay was obtained by precipitation with 50% saturated ammonium sulphate followed by DEAE-cellulose chromatography and Sephadex G-150 gel filtration. These purified IgG fraction was compared with Cappel company standard IgG fraction for ?? subpopulation assay. We used home-made IgG fraction and obtained favorable results in ?? subpopulation assay as Cappel company standard IgG fraction.
Epstein-Barr 바이러스 형질전환법을 이용한 파상풍 톡소이드에 대한 사람 단세포군 항체의 생산
유승민,조정제,호순태,하윤문 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
For production of tetanus toxoid(TT) specific human monoclonal antibodies, anti-TT antibody secreting peripheral B lymphocytes were separated by rosetting with TT-coated SRBC. And, B lymphoblastoid cell line(BLCL) was established by Epstein-Barr Virus(EBV) transformation methods. Stable BLCLs were established after three times of cloning by limiting dilution. To establish the maximal antibody producing condition, several environmental factors were tested. The optimal condition for maximum antibody prodiction was 4 day culture of 1 x 105cells/ml concentration in RPMI 1640 complete culture media supplemented with 20% fetal calf serum, and the maximum concentration of secreted antibody was about 900ng/ml. The antibody production of BLCL was decreased during long-term culture after establishment of cell line, but antibody production was maintained by repeated cloning by limiting dilution.
Case Report : Primary Squamous Cell Carcinoma of the Liver Initially Presenting with Pseudoachalasia
( Mun Ki Choi ),( Gwang Ha Kim ),( Geun Am Song ),( Hyung Seok Nam ),( Yang Seon Yi ),( Kang Hee Ahn ),( Suk Kim ),( Joo Yeun Kim ),( Do Youn Park ) The Editorial Office of Gut and Liver 2012 Gut and Liver Vol.6 No.2
Pseudoachalasia secondary to primary squamous cell carcinoma (SCC) of the liver is extremely rare and has not been reported until now. Here, we report a unique case of primary SCC of the liver initially presenting with progressive dysphagia along with short periods of signifi cant weight loss. A 58-yearold man initially presented with progressive dysphagia along with significant weight loss over brief periods of time. The radiographic and manometric fi ndings were consistent with achalasia. Subsequent esophagogastroduodenoscopy revealed a moderately dilated esophagus without evidence of neoplasm or organic obstruction. However, firm resistance was encountered while traversing the esophagogastric junction (EGJ), although no mucosal lesion was identifi ed. Due to the clinical suspicion of the presence of a malignant tumor, endoscopic ultrasonography (EUS) and computed tomography scans of the chest and abdomen were obtained. A huge hepatic mass with irregular margins extending to the EGJ was found. EUS-guided fine-needle aspiration was performed, and the mass was diagnosed as a primary SCC of the liver by immunohistochemical staining. (Gut Liver 2012;6:275-279)
韓國人 正常人의 T Lymphocytes와 T Subsets에 關한 硏究
夏潤文,金燦洙,李武炯,尹在一,林壽德 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1981 東西醫學硏究所 論文集 Vol.1 No.1
Peripheral blood from thirty(30) normal Korean subjects was examined for the proportional numbers of lymphocytes, T lymphocytes, and T subsets present. The method used for this investigation utilized the rosette technique by which the T lymphocyte bind naturally to sheep erythrocytes and T subpopulation bind to ox erythrocytes coated with rabbit Ig antibody. It was found that T lymphocytes were 73±4%(mean±S.D) in PBL freshly drawn from normal subjects, while its subpopulations EA(IgM)-RFC, ?? and EA(IgG), -RFC, ?? were present 42±7% and 10±3%(mean±S.D) respectively; but unlike EA(IgG)-RFC the EA(IgM)-RFC was present only in suspensions kept in culture for 18-20 hrs. Absolute number of lymphocytes in each cubic millimeter, and recovery of lymphocytes in PBL from normal individuals were 1962±406 and 59±15%(mean±S.D) respectively. This investigation showed that different receptors of human T cells were easily detected by the highly sensitive rosetting technique.