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Sung, Ha Chin,Chang, Hyo Ihl,Kim, Min Jung,Kim, Young Woo,Lee, Jin Young 생화학분자생물학회 1981 BMB Reports Vol.29 No.5
Bacteriophage ΦFCl is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage ΦFCl integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.
Sung, Ha Chin,Kim, Sang Uk,Yoon, Chang No,Chi, Myung Whan 생화학분자생물학회 1999 BMB Reports Vol.31 No.5
A new set of functional group interaction energy descriptors relevant to the ACE (Angiotensin-Converting Enzyme) inhibitory peptide, QSAR (Quantitative Structure Activity Relationships), is presented. The functional group interaction energies approximate the charged interactions and distances between functional groups in molecules. The effective energies of the computationally derived geometries are useful parameters for deriving 3D-QSAR models, especially in the absence of experimentally known active site conformation. ACE is a regulatory zinc protease in the renin-angiotensin system. Therapeutic inhibition of this enzyme has proven to be a very effective treatment for the management of hypertension. The nonbond interaction energy values among functional groups of six-feature of ACE inhibitory peptides were used as descriptor terms and analyzed for multivariate correlation with ACE inhibition activity. The functional group interaction energy descriptors used in the regression analysis were obtained by a series of inhibitor structures derived from molecular mechanics and semi-empirical calculations. The descriptors calculated using electrostatic and steric fields from the precisely defined functional group were sufficient to explain the biological activity of inhibitor. Application of the descriptors to the inhibition of ACE indicates that the derived QSAR has good predicting ability and provides insight into the mechanism of enzyme inhibition. The method, functional group interaction energy analysis, is expected to be applicable to predict enzyme inhibitory activity of the rationally designed inhibitors.
Analysis of high-fat diet-induced inflammatory responses in Rhbdf2 knockout mice
Sung-Jun Kim,Ki-Hoan Nam,Seul-Gi Park,Young-Sub Byun,Eun-Kyoung Kim,Sang-Mi Cho,Ha-rim-Kim,Hyoung-Chin Kim,Hu-Jang Lee,Beom Jun Lee 한국예방수의학회 2018 예방수의학회지 Vol.42 No.4
This study investigated the characteristics of obesity induced by a high-fat diet (HD) over 13 weeks in Rhbdf2 gene knockout (KO) mice. Forty 7-week-old Rhbdf2 wild and KO mice were used and the mice were divided into 4 groups: Wild-ND (n=10, Rhbdf2 wild mice, normal diet (ND)), Wild-HD (n=10, Rhbdf2 wild mice, HD), KO-ND (n=10, Rhbdf2 KO mice, ND) and KO-HD (n=10, Rhbdf2 KO mice, HD). The relative epididymal fat weight in KO-HD was significantly increased compared with that in KO-ND (P<0.01). The relative liver and spleen weights in KO-HD were decreased compared with those in Wild-HD (p < 0.05) and KO-ND (p < 0.01). The mRNA expression of SOD1 in KO-ND was significantly reduced compared with that in Wild-ND (p < 0.05). In Wild-ND and HD, the mRNA expressions of TNF-α and IL-6 in epididymal fat were significantly increased compared with those in KO-ND and HD (p < 0.01). A significant increase of TNF- α and IL-6 mRNA expression was observed in KO-HD compared with KO-ND (p < 0.01). These results indicated that Rhbdf2 genes may regulate high fat diet-induced obesity damage by anti-inflammatory and anti-oxidative roles in fat tissue of mice.
( Sang Hoon Yoo ),( Jae Ha Lee ),( Kwang Ha Yoo ),( Ki-suck Jung ),( Chin Kook Rhee ) 대한결핵 및 호흡기학회 2018 Tuberculosis and Respiratory Diseases Vol.81 No.3
Background: Chronic bronchitis (CB) is an important phenotype in chronic obstructive pulmonary disease (COPD). The purpose of this study is to evaluate different pattern of COPD assessment test (CAT) score between CB and non-CB patients. Methods: Patients were recruited from 45 centers in Korea, as part of the Korean COPD Subgroup Study cohort. CB was defined when sputum continued for at least 3 months. Results: Total 958 patients with COPD were eligible for analysis. Among enrolled patients, 328 (34.2%) were compatible with CB. The CAT score was significantly higher in patients with CB than non-CB, and each component of CAT score showed a similar result. CB was significantly associated with CAT score when adjusted with age, sex, modified Medical Research Council, and post-bronchodilator forced expiratory volume in 1 second. Each component of CAT score between patients with CB and non-CB showed different pattern according to Global Initiative for Chronic Obstructive Lung Disease grade. Conclusion: CAT score is significantly higher in patients with CB than non-CB. Each component of CAT score was significantly different between two groups.
Interconversion of Electronic Spin State of p-Substituted Arylketocarbene Reactions
Sung, Dae-Dong,Jeong, Jin-Hee,Ryu, Zoon-Ha,Chin, Won-Bae,Lee, Ik-Choon Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8
Rate constants for photolytic reactions of p-substituted 2-diazopropiophenones were determined in acetonitrile. The reactions show a comparatively low value of activation energy and activation enthalpy to alkylcarbenes or other arylcarbenes. The transition state corresponds to the step of a new carbonyl bond formation. The high negative ρ -values are shown in Hammett plots. The kinetics results and EPR spectrum are in accord with a phenomenon that occurs in interconversion between singlet and triplet carbenes.
Structural basis for Ufm1 processing by UfSP1.
Ha, Byung Hak,Ahn, Hee-Chul,Kang, Sung Hwan,Tanaka, Keiji,Chung, Chin Ha,Kim, Eunice Eun Kyeong American Society for Biochemistry and Molecular Bi 2008 The Journal of biological chemistry Vol.283 No.21
<P>Ubiquitin-fold modifier 1 (Ufm1) is a newly identified ubiquitin-like protein. Like ubiquitin and other ubiquitin-like proteins, Ufm1 is synthesized as a precursor that needs to be processed to expose the conserved C-terminal glycine prior to its conjugation to target proteins. Two novel proteases, named UfSP1 and UfSP2, have been shown to be responsible for the release of Ufm1 from Ufm1-conjugated cellular proteins as well as for the processing of its precursor. They show no sequence homology with known proteases. Here, we describe the 1.7A resolution crystal structure of mouse UfSP1, consisting of 217 amino acids. The structure reveals that it is a novel cysteine protease having a papain-like fold, with Cys(53), Asp(175), and His(177) that form a catalytic triad, and Tyr(41) that participates in the formation of the oxyanion hole. This differs from the canonical catalytic triad of papain-like proteases in that the aspartate and the histidine residues are from the 'Asp-Pro-His' box. The Asp-Pro-His configuration seen in UfSP1, together with Atg4B and M48(USP), seem to form a new subfamily of the cysteine protease superfamily. The mutagenesis study of the active site residues confirms structural basis for catalysis. The interaction between UfSP1 and Ufm1 appears quite substantial, since the K(D) value was estimated to be 1.6 mum by the isothermal titration calorimetry analysis. Furthermore, the NMR data shows that the loop between beta3 and alpha2 in addition to the C-terminal region of Ufm1 plays a role in binding to UfSP1.</P>
( Sang Soo Lee ),( Ra Ri Cha ),( Chang Min Lee ),( Wan Soo Kim ),( Hyun Chin Cho ),( Jin Joo Kim ),( Jae Min Lee ),( Hong Jun Kim ),( Chang Yoon Ha ),( Hyun Jin Kim ),( Tae Hyo Kim ),( Woon Tae Jung ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims:HepaticCvirus(HCV)genotype3infectionisthemostdifficultformofHCVtotreat,withamorerapidprogressiontofibrosisandcirrhosiscomparedwithothergenotypes.Theaimsofthisretrospectiveobservationalstudyweretoelucidatetheimpactofgenotype3infectiononhepatocellularcarcinoma(HCC)developmentandoverallmortalityinpatientswithHCV-relatedcirrhosis,comparedtoHCVgenotype1and2intheGyeongnamProvince,locatedonthesoutheastcoastofKorea.Methods:Atotal153patientswithHCV-relatedcirrhosiswereincludedbetweenJanuary2005andDecember2014.Amongthese,74hadgenotype1,55genotype2,and24patientshadgenotype3infection.Results:Theprevalenceofgenotype3inHCV-relatedcirrhosiswas16%.Individualsatriskforgenotype3infectionwereyoung,malegender,andpeoplewhoinjectdrugs.Duringmedianamedianfollow-up40.1months,38patientsdevelopedHCC,and23patientsdied.TheincidenceofHCCdevelopmentandoverallmortalitywassignificanthigherinpatientswithgenotype3comparedtopatientswithgenotype1and2inthelogranktest.Despitebeingyounger,HCVgenotype3wasaindependentriskfactorforHCCdevelopment(adjustedhazardratio[HR〕=2.55)andoverallmortality(adjustedHR=3.69)onmultivariateanlalysis.Afterexclusionofpatientswithachievedsustainedvirologicresponse,theincidenceofHCCdevelopmentandoverallmortalitywassignificanthigherinpatientswithgenotype3comparedtopatientswithnogenotype3.Conclusions:HCVgenotype3infectionisassociatedwithanincreasedriskofHCCandoverallmortalityinpatientswithcirrhosisafteradjustingwithconfoundingfactors.