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Hajin Nam,서준교,김진규 한국생약학회 2013 Natural Product Sciences Vol.19 No.3
The inhibitory effects of adipogenesis on ethyl acetate (EtOAc) fraction from leaves of the Tulip tree (TT) were evaluated. Exposure to TT EtOAc fraction (25~200 mg/mL) for a 72 hr incubation period did not significantly change cell viability. TT EtOAc fraction, with concentrations of 100 and 200 mg/mL, inhibited lipid accumulation in 3T3-L1 adipocytes in a dose dependent manner in adipogenesis. The expression of PPARg and C/EBPa, essential adipogenic markers, was significantly decreased when TT EtOAc fraction was added to cells for 8 days as compared with the untreated control group. These results suggest that TT EtOAc fraction might be a potential therapeutic agent as an effective, natural alternative material for obesity treatment.
Hajin Nam,김유연,김보영,윤원기,김형진,서준교 한국실험동물학회 2018 Laboratory Animal Research Vol.34 No.4
Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed to obtain data on the characteristics of a newly developing inbred strain (KWM/Hym) captured from Chuncheon, Korea. All of five Korean wild male mice have the zinc-finger Y (ZfY) gene. Also, all of 19 Korean wild mice used in this analysis have the AKV-type murine leukemia virus gene, indicating that Korean wild mice might be Mus musculus musculus. To identify the genetic polymorphism in KWM/Hym, SNP analysis was performed. In a comparison with 28 SNP markers, there was a considerable difference between KWM/Hym and several inbred strains. The homogeneity between KWM/Hym and the inbred strains was as follows: C57BL/6J (39.3%), BALB/c AJic (42.9%), and DBA/2J (50%). KWM/Hym is most similar to the PWK/PhJ inbred strain (96.4%) derived from wild mice (Czech Republic). To identify the morphometric characteristics of KWM/Hym, the external morphology was measured. The tail ratio of male and female was 79.60±3.09 and 73.55±6.14%, respectively. KWM/Hym has short and agouticolored hairs and its belly is white with golden hair. Taking these results together, KWM/Hym, a newly developing inbred mouse originated from wild mouse, might be use as new genetic resources to overcome the limitations of the current laboratory mice.
Nam, Hajin,Jung, Harry,Kim, Yooyeon,Kim, Boyoung,Kim, Kyeong Hee,Park, Sang Jae,Suh, Jun Gyo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
As the mechanism of aged black garlic (ABG) extract affecting lipid metabolism in adipocytes remains unclear, this study evaluated the effect of ABG extract on lipid metabolism and the expression of related proteins in mature 3T3-L1 adipocytes. ABG extract treatments at 0, 0.625, 1.25, and 2.5, and 5 mg/mL had no effect on cell morphology or viability in adipocytes. ABG extract suppressed lipogenesis and induced lipolysis in a dose-dependent manner compared to control. Furthermore, ABG extract at 2.5 and 5 mg/mL significantly reduced protein expression of proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$) and perilipin in mature 3T3-L1 adipocytes. The hormone-sensitive lipase (HSL) and $Ser^{563}-pHSL$ levels were also significantly reduced by treatment with 5 mg/mL of ABG extract. Taken together, these results suggest that ABG extract has anti-lipogenic and lipolytic effects in mature 3T3-L1 adipocytes, indicating a potential in anti-obesity therapies.