Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults

Shin, M.J.,Kim, D.W.,Jo, H.S.,Cho, S.B.,Park, J.H.,Lee, C.H.,Yeo, E.J.,Choi, Y.J.,Kim, J.A.,Hwang, J.S.,Sohn, E.J.,Jeong, J.H.,Kim, D.S.,Kwon, H.Y.,Cho, Y.J.,Lee, K.,Han, K.H.,Park, J.,Eum, W.S.,Choi, Pergamon ; Elsevier Science Ltd 2016 FREE RADICAL BIOLOGY AND MEDICINE Vol.97 No.-

Proline rich Akt substrate (PRAS40) is a component of mammalian target of rapamycin complex 1 (mTORC1) and is known to play an important role against reactive oxygen species-induced cell death. However, the precise function of PRAS40 in ischemia remains unclear. Thus, we investigated whether Tat-PRAS40, a cell-permeable fusion protein, has a protective function against oxidative stress-induced hippocampal neuronal (HT-22) cell death in an animal model of ischemia. We showed that Tat-PRAS40 transduced into HT-22 cells, and significantly protected against cell death by reducing the levels of H<SUB>2</SUB>O<SUB>2</SUB> and derived reactive species, and DNA fragmentation as well as via the regulation of Bcl-2, Bax, and caspase 3 expression levels in H<SUB>2</SUB>O<SUB>2</SUB> treated cells. Also, we showed that transduced Tat-PARS40 protein markedly increased phosphorylated RRAS40 expression levels and 14-3-3σ complex via the Akt signaling pathway. In an animal ischemia model, Tat-PRAS40 effectively transduced into the hippocampus in animal brain and significantly protected against neuronal cell death in the CA1 region. We showed that Tat-PRAS40 protein effectively transduced into hippocampal neuronal cells and markedly protected against neuronal cell damage. Therefore, we suggest that Tat-PRAS40 protein may be used as a therapeutic protein for ischemia and oxidative stress-induced brain disorders.

Promotional effects of oxygen-containing additives on ammonia borane dehydrogenation for polymer electrolyte membrane fuel cell applications

Yeo, S.,Kim, Y.,Lee, J.H.,Kim, K.,Jang, J.H.,Hong, S.A.,Nam, S.W.,Yoon, C.W. Pergamon Press ; Elsevier Science Ltd 2014 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.39 No.36

To develop continuous hydrogen generators utilizing ammonia borane (AB), one of the extensively studied chemical hydrogen storage material for applications in polymer electrolyte membrane fuel cells (PEMFCs), various types of oxygen-containing compounds such as 18-crown-6, 18-crown-6 derivatives, glycols, and polyethylene glycols were examined as chemical additives to enhance the H<SUB>2</SUB>-release properties of AB. The rate and extent of AB dehydrogenation with these promoters were found to increase considerably at temperatures ranging from 85 <SUP>o</SUP>C to 125 <SUP>o</SUP>C; in particular, a mixture of AB and either 18-crown-6 or tetraethylene glycol afforded a material-based hydrogen storage capacity of >9.5 wt%. In situ Fourier transform infrared (FT-IR) and solid-state nuclear magnetic resonance (NMR) spectroscopic studies were conducted to identify gaseous byproducts potentially detrimental to a PEMFC. Density functional theory (DFT) studies demonstrated that the additives could interact with AB via hydrogen bonding between oxygen atoms of a promoter and N-H of AB, which could ultimately facilitate AB dehydrogenation.

Effect of heat treatment temperature and time on sound absorption coefficient of Larix kaempferi wood

Chung, H.,Park, Y.,Yang, S. Y.,Kim, H.,Han, Y.,Chang, Y. S.,Yeo, H. Springer Science + Business Media 2017 Journal of wood science Vol.63 No.6

<P>Heat treatment improves the dimensional stability and hydrophobicity of wood, and heat-treated wood is currently attracting attention as a new interior material. However, there are few evaluations where the acoustic properties of heat-treated wood are reported when such wood is used as an interior material. In this study, Larix kaempferi wood, typically used as a building material, was heat-treated at 200, 220, and 240 A degrees C for 9, 12, 15, and 18 h. The sound absorption coefficients of the treated wood samples were measured at 250, 500, 1000, 2000, and 4000 Hz in a reverberation room. The sound absorption coefficient increased with the treatment temperature and the treatment time. The results of this study showed that the high-frequency band range sound absorption coefficient of wood can be increased dramatically by heat treatment.</P>

Highly-conformal nanocrystalline molybdenum nitride thin films by atomic layer deposition as a diffusion barrier against Cu

Jang, Y.,Kim, J.B.,Hong, T.E.,Yeo, S.J.,Lee, S.,Jung, E.A.,Park, B.K.,Chung, T.M.,Kim, C.G.,Lee, D.J.,Lee, H.B.R.,Kim, S.H. Elsevier Sequoia 2016 JOURNAL OF ALLOYS AND COMPOUNDS Vol.663 No.-

Molybdenum nitride (Mo<SUB>2</SUB>N) thin films were grown by atomic layer deposition (ALD) using a sequential supply of a newly synthesized Mo metalorgranic precursor, Mo(N<SUP>t</SUP>Bu)<SUB>2</SUB>(S<SUP>t</SUP>Bu)<SUB>2</SUB>, and H<SUB>2</SUB> plasma at a substrate temperature of 300 <SUP>o</SUP>C. A newly proposed ALD system exhibited typical ALD characteristics, such as a self-limited film growth and a linear dependency of the film thickness on the number of ALD cycles, and showed a growth rate of 0.028 nm/cycle on a thermally grown SiO<SUB>2</SUB> substrate. Such the ideal ALD growth characteristics enabled excellent step coverage of ~80% for the ALD-grown Mo<SUB>2</SUB>N film onto nano-trenches with a width of 25 nm and an aspect ratio ~4.5. The optimized film had a resistivity as low as ~350 μΩ-cm. X-ray diffraction, Rutherford backscattering spectrometry, and X-ray photoelectron spectroscopy analyses confirmed that formation of N-rich cubic Mo<SUB>2</SUB>N (N/Mo = ~0.7) phase with carbon and sulfur impurities of 2.6 and 7.4 at.%, respectively. Plan-view transmission electron microscopy analysis showed that the film formed a nanocrystalline microstructure with 5-8-nm-sized grains embedded in an amorphous matrix. An ultrathin (only ~4 nm-thick) ALD-grown Mo<SUB>2</SUB>N film effectively prevented diffusion of Cu into Si after annealing at a temperature even up to 650 <SUP>o</SUP>C.

Changes of major chemical components in larch wood through combined treatment of drying and heat treatment using superheated steam

Park, Y.,Jang, S. K.,Park, J. H.,Yang, S. Y.,Chung, H.,Han, Y.,Chang, Y. S.,Choi, I. G.,Yeo, H. Springer Science + Business Media 2017 Journal of wood science Vol.63 No.6

<P>The effects of the combined treatment of drying and heat treatment using superheated steam (SHS) were studied relative to the changes of the major chemical components in larch wood. The green lumber was dried and heat-treated in SHS conditions of 250 A degrees C and 0.5 MPa for 18 h, and the relative percentage contents of sugars, lignin, and extractives were investigated and compared with the relative percentage contents in the lumber heat-treated in hot air conditions of 250 A degrees C and atmospheric pressure for 18 h. After both heat treatment methods, the relative percentage contents of xylan, mannan, galactan, and arabinan were greatly decreased, whereas that of the Klason lignin was increased, additionally that of glucan and extractives remained almost unchanged. Lignin may bind with furan compounds decomposed from hemicellulose following heat treatment, thus contributing to the increase in the apparent relative percentage contents of the Klason lignin. In addition, the condensate collected in the condenser after combined drying and heat treatment using SHS was investigated qualitatively and quantitatively by high-performance liquid chromatography (HPLC). A large amount of furfural and acetic acid decomposed from hemicellulose was detected and some sugar components composed of cellulose and hemicellulose were detected in the liquid condensate.</P>

Evolution of implanted Fe ions in SiO₂/Si wafer into uniformly sized catalyst particles for carbon nanotube forest growth

Lee, C.H.,Lee, J.,Yeo, S.,Lee, S.H.,Kim, T.,Cha, H.G.,Eun, Y.,Park, H.J.,Kim, S.M.,Lee, K.H. Pergamon Press ; Elsevier Science Ltd 2017 Carbon Vol.123 No.-

<P>We report the synthesis of carbon nanotube (CNT) forests with a narrow diameter distribution based on Fe ion implantation method. By annealing the Fe-implanted SiO2/Si wafer in an Ar atmosphere at 800 degrees C for 15 min, the Fe particles on the surface of SiO2 layer are successfully formed by the diffusion of Fe atoms from the SiO2 layer. Interestingly, the size distribution of Fe catalyst particles for Fe-implanted SiO2/Si wafers does not change with the prolonged annealing durations of up to 12 h. Using secondary ion mass spectroscopy and transmission electron microscopy (TEM), we confirmed that the implanted Fe atoms diffuse out of the SiO2 layer and form Fe particles on both the SiO2 surface and the interface between SiO2 and Si. The cross-sectional TEM images indicate that the Fe catalyst particles are anchored in the SiO2 layer, which limits the particles' mobility and results in an invariant catalyst size distribution for prolonged annealing durations. Therefore, we anticipate that implantation can be an efficient alternative catalyst preparation method for CNT forest growth which can solve various growth issues that are inherently caused by conventional physical vapor deposition method. (C) 2017 Elsevier Ltd. All rights reserved.</P>

재래돼지(Korea Native Pigs) 특이 genetic marker와 육질 연관성 분석

김철욱,여정수,조광근,진상근,오명곤,박준규,권은정,홍연희,김지현,이보경,박다혜,김재우,이지홍 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6

본 연구는 재래돼지 특이적인 marker를 개발하고 이들 marker와 육질과의 연관성을 규명하기 위하여 실시하였다. 실험동물로 재래돼지와 랜드레이스 각각 30두씩 총 60두를 사용하였으며, 품종 특이 marker를 찾기 위하여 4번과 7번 염색체에 존재하는 총 60개의 microsatellite를 이용하여 PCR (polymerase chain reaction)을 수행하였다. PCR 증폭은 형광물질 Fam과 Hex, Ned로 표지된 한쌍의 microsatellite primer를 이용하였으며, 형광물질로 표식된 PCR 산물은 Genetic Analyzer ABI 310으로 전기영동하여 대립 유전자를 규명하고 통계 분석하였다. 대립 유전자에 대한 통계 분석 결과 총 60개의 microsatellite중 27개가 다양한 유전적 다형현상을 보이는 대립 유전자가 증폭되었으며 (p<0.05), 특히 SW1364와 SW445, SW1369, SWR773 microsatellite의 증폭 산물에서 재래돼지와 랜드레이스 두 품종간을 구분할 수 있는 품종 특이적인 대립 유전자를 확인하였다 (p<0.01). 또한 육질에 영향을 미치는 유전적 marker를 얻기 위해 60두 전체에 대한 육색, 지방색, 지방산 조성, 콜레스테롤 함량, 연도와 경도에 대한 육질 분석을 실시하여 육질 형질과 marker와의 연관성을 분석한 결과 육질 형질에 영향을 미치는 28개 marker를 확인하였으며, 육색과 지방색에 연관된 4개, 조직감과 연관된 10개의 marker를 확인하였다. 이렇게 선발된 marker들은 고급육 생산을 위한 돼지의 유전적 개량을 위하여 유용하게 이용될 수 있다. This study was conducted to develop Korean native pig-specific DNA markers and analyze its relativity with meat quality using 30 of Korean native pigs and Landraces, respectively. To find species-specific DNA markers, we selected and tested a total of 60 microsatellite markers on the 4 and 7 chromosome using polymerase chain reaction (PCR). PCR amplification was carried out by microsatellite primer pairs labeled with fluorescent Fam, Hex and Ned. The fluorescent labeled PCR products were electrophoresed with Genetic Analyzer ABI 310 followed by the allelic and statistical analysis. According to the allelic frequency annalysis, of the 60 microsatellite markers 27 were preduced with a variety of genetic polymorphic alleles (p<0.05). Especially, alleles of SW1364, SW445, SW1369 and SWR773 microsatellite markers were identified to be species-specific markers between two breeds (p<0.01). To analyze the meat quality we determined the meat color, fat color, fat composition, cholesterol contents and hardness, and compared these characteristics with the alleles of 36 species-specific markers in all pigs. Of the 60 microsatellite markers 28 were found to be related with meat quality; 18 for meat and fat color, 7 for fatty acid, 4 for cholesterol contents and 10 for shear force and hardness. These selected markers will be useful in the genetic improvement of pigs for an excellent meat quality.

피부 섬유아세포에서 진달래 추출물의 항산화 및 항노화 효능

최선일 ( Choi S. I. ),이사라 ( S. Lee ),이혜진 ( H. J. Lee ),김병직 ( B. J. Kim ),여주홍 ( J. Yeo ),정태동 ( T. D. Jung ),조봉연 ( B. Y. Cho ),최승현 ( S. H. Choi ),한웅호 ( X. Han ),심완섭 ( W. S. Sim ),이진하 ( J. H. Lee ),이옥환 ( O. 강원대학교 농업생명과학연구원(구 농업과학연구소) 2017 강원 농업생명환경연구 Vol.29 No.3

현재 식품 공전에 식품원료로 사용이 가능한 것으로 등록된 국내 산림지역 자생 식물을 식품산업에 활용하고자 국내 자생식물 45종을 선별하였고, 본 연구팀의 선행연구에서 45종의 항산화 활성을 평가한 결과, 다른 종들과 비교하여 우수한 항산화 활성을 보인 진달래를 본 연구에서 사용하였다. 피부 섬유아세포에 Hydrogen peroxide로 산화적 스트레스를 유도한 상태에서 진달래 추출물의 세포 보호효과 및 세포내 항산화 효과를 관찰하였다. 세포 보호효과를 측정한 결과 Hydrogen peroxide로 인해 세포 생존율이 감소한 반면 진달래 추출물처리로 세포의 산화적 손상이 억제되는 것으로 관찰되었다. H2-DCFDA 염색을 통해 세포내 항산화 효과를 확인한 결과 진달래 추출물에 의한 활성산소 저감을 확인하였다. 또한 SA-β-galactosidase assay를 통해 항노화 효과를 확인한 결과 진달래 추출물을 처리했을 때 세포의 SA-β-galactosidase 활이 줄어드는 것을 확인할 수 있었다. 이상의 결과를 종합하여 볼 때 진달래 추출이 Hydrogen peroxide로 산화적 스트레스를 유도한 상태에서 세포 생존율을 증가시키고 세포내 활성산소의 생성을 억제하며 이로 인해 세포 노화억제가 관찰되어 천연물에서 유래한 기능성 식품원료로서의 활용도가 매우 넓을 것으로 판단된다. Skin aging is associated with increased reactive oxygen species (ROS) production by human cells, which is characterized by wrinkles and atypical pigmentation. Use of antioxidants is an effective approach to prevent symptoms associated with ROS-induced skin aging. Although synthetic antioxidants have been used to remove free radicals, several studies have reported that the synthetic antioxidants have side effects that cause long-term edema, which subsequently results in cancer and intracellular toxicity. Therefore, the study of safe and functional antioxidants derived from natural products is necessary for replacement of synthetic antioxidants. In this study, the antioxidant and anti-aging effect of Rhododendron mucronulatum Turcz. extracts (RTE) on human dermal fibroblasts (HDFs) were investigated. We confirmed that RTE markedly reduced hydrogen peroxide-induced cell damage, intracellular ROS, and oxidative stress-induced senescence in HDFs. These results suggest that RTE is potential natural source of antioxidant and anti-aging compounds.

한우의 근내지방도에 연관된 DNA Marker 의 규명

여정수,김재우,이문연,이지홍,강태석 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.5

DNA markers related to marbling score were identified using DNA fingerprinting with M13 probe and restriction enzyme HaeⅢ from 336 heads(147 heads of bull and 189 heads of steer) of Hanwoo. It was found that distinct 2 kinds of specific markers at 9.4 and 3.6kb showing significant difference for marbling score. DNA Marker groups having either 9.4, 3.6 or 9.4/3.6kb showed higher frequency of meat quality grade 1 or 2 than the group having none-marker. Also, the group having 9.4kb showed 94.4% of grade 1, indicating that this marker is valuable for the improvement of meat quality and was able to separate completely the lowest beef grade no.3.

한우의 일당 증체량에 연관된 DNA Marker 의 규명

여정수,박노형,김재우,이문연,장태경 한국축산학회 1999 한국축산학회지 Vol.41 No.4

DNA markers related to daily gain from 287 heads( 173 heads of bull and 114 heads of steer) of Hanwoo were found with distinct 3 kinds at 4.6, 3.6 and 2.8kb using DNA fingerprinting with M13/HaeIII and 2 kinds at 5.6 and 2.1kb with M13/Hinfl. It was evidently considered that 3 specific markers influenced to grow in Hanwoo, negative effect in 4.bkb and positive in o hers(3.6 and 2.8kbs) for daily gain and especially single effect at 3.bkb for bulls and combined effect of 4.6 arid 2.8 markers for steers were distinctive to daily gain with M13/HaeIII. Pedigreed pattern of specific markers with M13/HaeⅢ were observed with DNA bands at 2.8 and 3.bkb were dependent on sire. Under results of this research culling of 4.bkb marker and selection of 3.bkb marker in Hanwoo population can be expected to improve above 35㎏ at market body weight.