α-Methyl Dl-N-Benzylaspartate의 합성

이채호,조성동,이만구,채규윤 圓光大學校 基礎自然科學硏究所 1987 基礎科學硏究誌 Vol.6 No.1

Synthesis of α-methyl N-benzylaspartates from DL-aspartic acid is described. DL-Aspartic acid was transformed in several ways into DL-aspartic anhydride which was subjected to alcoholysis in methanol. Benzylation of the resulting a-methyl DL-aspartate with benzyl bromide produced a-methyl β-benzyl DL-N-benzylaspartate. Hydrogenolysis of this ester was converted to α-methyl DL-N-benzylaspartate.

Diethyl 3,4,5, - Trimethoxybenzylmalonate의 새로운 합성법

李茶浩,李英行,李晩求,蔡奎允 圓光大學校 1984 論文集 Vol.18 No.2

3,4,5 - Trimethoxybenjoyl chloride 와 diethyl malonate를 축합시키고 이어서 염소화반응 및 수소화반응에 의하여 70%의 수득률로서 diethyl 3,4,5 - trimethoxybenzylmalonate를 얻었다. direct condensation of 3,4,5 - trimethoxybenzoyl chloried with diethyl malonate followed by chlorination and hydrogenation afforded diethyl 3,4,5 - trimethoxybenzylmalonate in 70% overall yield.

α-Alkyl N-Benzylaspartates의 합성

이채호,이만구,채규윤 圓光大學校 基礎自然科學硏究所 1986 基礎科學硏究誌 Vol.5 No.2

Synthesis of α-alkyl N-benzylates from maleic anhydride is described. Maleic anhydride was transformed into monoalkyl maleates which were treated which benzylamine to afford β-alkyl N-benzylaspartates(alkyl=methyl and benzyl). Base hydrolysis of these esters and subsequent treatment of the resulting carboxylic acid with HBr in AcOH produced N-benzylaspartic anhydride hydrobromide. Alcoholysis of this salts in methanol and benzyl alchol yielded α-methyl N-benzyl-aspartate and α-benzyl N-benzylaspartate, respectively.

Ethyl 4-Carbetoxy-1, 1-dioxo-5, 6-2H-1, 2, 6-thiadiazin-5-ylacetate로 부터 N-Alky1-3-carbetoxy-2-pyridinones의 합성

李采浩,鄭永洙,李英行,蔡圭允,崔昌鎭 圓光大學校 基礎自然科學硏究所 1992 基礎科學硏究誌 Vol.11 No.1

상이동 촉매를 이용한 β-lactams 합성

최창진,이영행,이채호,김쌍오,채규윤 圓光大學校 基礎自然科學硏究所 1987 基礎科學硏究誌 Vol.6 No.2

The reaction of N-benzyl β-amino acids with benzensulfonyl chloride in a chloroform-water bilayer system in the present of 15 mole % of tetrabutylammonium hydrogen sulfate gives the corresponding β-lactams in good yields.

Molecular Topography of Oat Phytochrome (124-kDa) Deduced by the Fluorescence Quenching

Chai, Young Gyu 漢陽大學校 基礎科學硏究所 1991 基礎科學論文集 Vol.10 No.-

빛에 의하여 식물의 광형태발생을 조절하는 파이토크롬은 적색광에 의하여 생물학적으로 활성이 있는 파이토크롬(Pfr)이 괴고 근적색광에 의하여 활성이 없는 파이토크롬 (Pr)이 된다. 하나의 단백질이지만, 빛에 의하여 변하는 Pr과Pfr 파이토크롬은 무엇이 어떻게 다른지를 그 단백질 내부에 존재하는 트립토판을 이용하여 형광 소광 방법을 도입하여 조사하였다. 소광제로 iodide를 사용한 결과 Pr이 Pfr보다 소광상수가 약 1.2등 정도 컸고, Pr이 Pfr보다 iodide에 어 노출되어 있으며, 파이토크롬의 chromophore가 있는 부분(Trp-366)이 Pr에서 Pfr로 광전환 반응이 일어 날 때 트립토판이 덮효 적게 노출되는 부위라 추론된다. The photoransformation of the photomorphogenic photoreceptor phytochrome was probed by the intrinsic luminescence of the tryptophan ????? residues. Trp surface topography of the red-and far-red-absorbing forms of phytochrome (Pr, Pfr) of Avenas ativa L. has been investigated by analyzing quenching of Trp fluorescence, in order to understand the differences in the two forms at the molecular level. Stern-Volmer kinetic analysis of the quenching date for quencher ????, showed a substantial exposure of the Trp residues in the Pr form (Stern-Volmer constants, Ksv, ??????), compared to those in the Pfr form (Ksv, ??????). A modified stern-volmer plot of the quenching data further confirms preferential exposure of the Trp residues in the Pr form (59%“exposed”Trp residues in the Pr form as compared to 46% in the Pfr form). The results indicate that most, it not all the ten Trp residues of phytochrome, are fluorescent and exist in distinct groups differing in their topography and microenvironment, and the peptied segment containing Trp-366 within the chromophore domain of phytochrome also undergoes a subtle alteration in its surface topography during Pr to Pfr phototransformation.

Rapid Detection of H - RAS Point Mutation Using Two - Step Polymerase Chain Reaction - Restriction Fragment Length Polymorphism

Chai, Young Gyu,Park, Young Suk,Lee, Kyung Ok 생화학분자생물학회 1981 BMB Reports Vol.29 No.5

Mutations in codon 12, 13 and 61 of one of the three ras genes, H-ras, K-ras and N-ras, convert these genes into active oncogenes. The presence of H-ras gene mutations have important prognostic implications in various cancers. In this study, the H-ras gene mutations were investigated by two-step PCRRFLP in patients with bladder and stomach cancer. For the control experiments, T24 and SK2 cell lines were used. In a total of 36 bladder cancer patient cases, five (13.9%) mutations were found by this method. Of these, point 12 mutations were two (5.6%) cases and point 61 mutations were three (8.3%) cases. On the other hand, H-ras mutation was not found in 29 cases of stomach cancer. The results of the mutated H-ras gene confirmed by direct sequencing analysis were con-elated well with PCR analysis. From the sensitivity test, the H-ras mutation was found to have about 0.2% of mutated DNA mingled in normal DNA. In conclusion, the H-ras mutation has a higher clinical significance in bladder cancer than stomach cancer. Moreover the two-step PCR-RFLP method is sensitive, rapid and relatively simple for clinical work in detecting H-ras point mutations.

Purification and Characterization of Bile Salt Hydrolase from Lactobacillus plantarum CK 102

( Chul Gyu Ha ),( Jin Kook Cho ),( Young Gyu Chai ),( Young Ae Ha ),( Shang Hun Shin ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.7

원격전이의 증거가 없는 식도암의 방사선치료 성적

채규영(Gyu Young Chai),장정순(Jeong Soon Jang),이종석(Jong Seok Lee) 대한방사선종양학회 1995 Radiation Oncology Journal Vol.13 No.1

Purpose : This study was done to evaluate preliminarily the role of intraluminal brachytherapy in the radiation treatment of non-metastatic esophageal cancer. Methrials and Methods : We analyzed follow-up result of 21 patients treated at the dept, of therapeutic radiology in Gyeongsang national university hospital between April, 1989 and August, 1992. All patients received neoadjuvant chemotherapy(%-FU, Cispl-atin). Fifteen patients were treated with external beam alone and in ramaining 6 patients, the external beam radiotherapy followed by intraluminal brachytherapy was done. Results : Among 21 patients, 7 patients showed complete tumor regression after completion of radiotherapy. But 2 of these complete responder recurred at the site of primary disease, so ultimate local control rate was 23.8%(5/21). Local control rate according to radiation treatment modality was 6.7%(1/15) in patients treated with external irradiation only, and 66.7% in patients treated with combined external irradiation and intraluminal brachytherapy. The 2 year NED survival rate was 6.6% in the former and 66.7% in the latter. Conclusion: Although there should be consideration about case selection for addition of intraluminal brachytherapy, intraluminal brachytherapy may be considered as on of the method to enhance the local control probability of esophageal cancer.

자궁경부암에 있어서 방사선치료 후의 치료실패 분석

채규영(Gyu Young Chai),강기문(Ki Mun Kang),이종학(Jong Hak Lee) 대한방사선종양학회 2001 Radiation Oncology Journal Vol.19 No.3

목 적 :방사선단독으로 치료했던 자궁경부암에서 치료실패 양상과 치료실패에 대한 위험인자를 확인하여 동시항암화학방사선요법의 적용기준을 마련하고자 본 연구를 시행하였다. 대상 및 방법 : 1989년 4월부터 1997년 12월까지 경상대학교병원 치료방사선과에서 자궁경부암으로 방사선치료를 시행받은 환자중 외부방사선조사와 강내조사를 계획대로 완료한 154명을 대상으로 후향적 분석을 시행하였다. FIGO에 의한 병기별 분포는 Ib 12명, IIa 24명, IIb 98명, IIIa 1명, IIIb 17명, IVa 2명이었다. 생존율은 Kaplan-Meyer 법을 이용하여 구하였고, 생존율의 비교는 Log- rank test로, 다변량분석은 Cox proportional hazard model을 이용하였 다. 국소 또는 원격실패에 대한 단변량, 다변량분석은 logistic regression model을 사용하였다. 방광 및 직장의 합병 증 평가는 RTOG/EORTC에서 제안하여 사용하고 있는 SOMA scale을 적용하였다. 결 과 :전체 154명 가운데 완전 관해를 보인 경우는 130명으로 완전관해율은 84.4%였다. 완전관해자 가운데 6명이 국소재발, 25명이 원격전이, 10명이 국소재발 및 원격전이를 보여 완전관해자 가운데 31.5%가 치료에 실패하였다. 연구대상 전체의 치료실패양상을 보면 국소실패가 25명, 원격실패가 25명, 국소 및 원격실패가 15명으로 전체환자의 치료실패율은 42.1% (65/154)였고, 국소실패율(국소단독실패와 국소, 원격 이중실패를 합한 것) 및 원격실패율(원격단독실패와 국소, 원격 이중실패를 합한 것)은 각각 25.9% (40/154), 25.9% (40/154)였다. 국소실패의 위험인자로서는 단변량, 다변량분석 모두에서 종양의 크기가 유의하였고 원격실패의 위험인자로서는 단변량분석에서는 병리, 종양의 크기, 골반임파절전이, 치료전 혈색소 수치가 유의하였으나, 다변량분석에서는 종양의 크기, 골반임파절전이가 유의하였다. 5년 생존율은 FIGO Ib 74%, IIa 67%, IIb 63%, IIIb 45%였다. 결 론 :종양의 크기가 4 cm 이상인 경우 방사선치료만으로는 국소 및 원격실패의 가능성이 높고 생존율 역시 낮다. 크기가 4 cm 이상이거나 골반임파절전이가 있는 경우 대동맥임파절 전이 가능성이 높다. 따라서 크기가 4 cm이상이거나 골반임파절 전이가 있는 경우 국소제어율을 높이고 원격전이를 줄이기 위해 동시방사선항암화학요법을 시행하여야 한다. Purpose : The aim of this study is to analyze the treatment failure patterns and the risk factors for locoregional or distant failure of uterine cervica l carcinoma treated with radiation therapy. Materials and methods : A retrospective analys is was undertaken of 154 patients treated with curative radiation thera py in Gyeongsang National Univers ity Hospital from April 1989 through December 1997. According to FIGO class ification, 12 patients were stage IB, 24 were IIA, 98 were IIB, 1 were IIIA, 17 were IIIB, 2 were IVA. Results :Overall treatment failure rate was 42.1% (65/154), and that of complete responder was 31.5%(41/130). Among 65 failures , 25 failed locoregionally, another 25 failed distantly, and 15 failed locoregionally and distantly. Multivariate analysis confirmed tumor size (>4 cm) as risk factor for locoregional failure, and tumor s ize (>4 cm), pelvic lymph node involvement as risk factors for distant failure. Conclusion :On the bas is of results of our study and recent published data of prospective randomized study for locally adva nced uterine cervical carcinoma, we concluded that uterine cervical carcinoma with size more than 4 cm or pelvic lymph node involvement should be treated with concurrent chemoradiation.