[P10-164] Isoliquiritigenine inhibits the migration adhesion and invasion of prostate cancer cells.

Gyoo Taik Kwon,Han Jin Cho,Soon Sung Lim,Won Yoon Chung,Aree Moon,Jung Han Yoon Park 한국식품영양과학회 2006 한국식품영양과학회 학술대회발표집 Vol.2006 No.10

A Study on the Improvement of Black Raspberry Extract for Andropause

Su jung Lee,Gyoo Taik Kwon,Hye Ran Choi,Sung Woong Gim 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

장내 미생물 다양성을 위한 Prebiotic 소재로서의 갈색거저리 유충 발효물의 가치

이수정(Su Jung Lee),권규택(Gyoo Taik Kwon),정이형(Yi Hyung Chung),유종상(Jong-Sang Yoo),조경훈(Kyung-Hoon Cho),김용석(Young-Suk Kim),장한수(Han Su Jang) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.6

곤충은 세계의 중요한 미래 산업 자원으로 주목받고 있다. 특히 한국에서 곤충은 “곤충산업법”으로 불리는 정부 지원정책으로 대중의 관심을 모으고 있다. 곤충은 천연식품으로 단백질, 아미노산, 비타민 B 등의 영양적 가치가 높고 동물사료에도 적합하다. 현재 귀뚜라미와 갈색거저리 유충(밀웜)이 널리 이용되고 있다. 본 연구에서는 갈색거저리 유충을 이용하여 발효과정을 거친 후 아미노산의 변화를 확인하였다. 또한 발효물을 이용한 사료를 제작하여 8주 동안 BALB/c 마우스에게 제공한 뒤 장내 미생물 분석을 시행하였다. 사료를 8주 동안 제공했을 때 체중의 변화는 없었다. 각 그룹의 장내 미생물 전체 수는 변하지 않았다. 일반세균, 대장균 및 대장균군 모두 그룹 간 어떠한 변화도 보이지 않았으나, FM 그룹에서 장내 유산균의 비율이 현저히 증가하였고 장내 미생물의 다양성을 증가시키는 것으로 확인되었다. 이러한 결과는 갈색거저리 유충 발효물이 인간의 장내 환경개선을 위한 prebiotics로서 가능성을 시사하지만, 인간에 대한 추가 적용 연구가 필요할 것으로 판단된다. Insects are attracting worldwide attention as important future industrial resources. Insect farming attracts public attention for commercialization especially in Korea, partly because of government subsidies and also support based on the legislation of the so-called “insect industry law”. Insects have a high nutritional value and contain protein, amino acids, vitamin B, etc., and are suitable for animal feed following the natural food web. Currently, crickets and mealworms are the most widely utilized in this regard. In this study, we measured amino acid changes in fermented mealworms (Tenebrio molitor L.). In addition, control, positive, and fermented mealworm (FM) diets were provided to BALB/c mice for 8 weeks. There was no change in dietary and body weight during the 8 weeks. The number of lactic acid bacteria in the intestines significantly increased in the FM group. There was no change in the total number of microbiota in each group nor was there any change in the general bacteria, E. coli, and E. coli form. These results suggest that fermented mealworm can be considered as a dietary prebiotic to improve the microbiota diversity of the human intestinal environment. However, further study for human application is necessary.

Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest

Cho, Han Jin,Lim, Do Young,Kwon, Gyoo Taik,Kim, Ji Hee,Huang, Zunnan,Song, Hyerim,Oh, Yoon Sin,Kang, Young-Hee,Lee, Ki Won,Dong, Zigang,Park, Jung Han Yoon MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.2

<P>Benzyl isothiocyanate (BITC) is a hydrolysis product of glucotropaeolin, a compound found in cruciferous vegetables, and has been shown to have anti-tumor properties. In the present study, we investigated whether BITC inhibits the development of prostate cancer in the transgenic adenocarcinoma mouse prostate (TRAMP) mice. Five-week old, male TRAMP mice and their nontransgenic littermates were gavage-fed with 0, 5, or 10 mg/kg of BITC every day for 19 weeks. The weight of the genitourinary tract increased markedly in TRAMP mice and this increase was suppressed significantly by BITC feeding. H and E staining of the dorsolateral lobes of the prostate demonstrated that well-differentiated carcinoma (WDC) was a predominant feature in the TRAMP mice. The number of lobes with WDC was reduced by BITC feeding while that of lobes with prostatic intraepithelial neoplasia was increased. BITC feeding reduced the number of cells expressing Ki67 (a proliferation marker), cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 in the prostatic tissue. <I>In vitro</I> cell culture results revealed that BITC decreased DNA synthesis, as well as CDK2 and CDK4 activity in TRAMP-C2 mouse prostate cancer cells. These results indicate that inhibition of cell cycle progression contributes to the inhibition of prostate cancer development in TRAMP mice treated with BITC.</P>

Sensitive Fern (<i>Onoclea sensibilis</i>) Extract Suppresses Proliferation and Migration of Vascular Smooth Muscle Cells Inflamed by Neighboring Macrophages

Kang, Sang-Wook,Kim, Jung-Lye,Kwon, Gyoo Taik,Lee, Yong-Jin,Park, Jung Han Yoon,Lim, Soon Sung,Kang, Young-Hee Pharmaceutical Society of Japan 2011 Biological & pharmaceutical bulletin Vol.34 No.11

<P>Vascular smooth muscle cell (SMC) migration and proliferation contribute to arterial wound repair and thickening of the intimal layer in atherosclerosis. SMC can physically interact with monocytes and macrophages within the intima. This study evaluated whether macrophages modulated proliferation and migration of SMC in close proximity, which was suppressed by 1—25 μg/ml sensitive fern (<I>Onoclea sensibilis</I>) extract (SFE) inhibiting protein-tyrosine phosphatase-1B activity. The addition of conditioned media of THP-1-derived macrophages substantially promoted human aortic smooth muscle cell (HAoSMC) proliferation by ≈30%. HAoSMC proliferation was significantly attenuated by ≥10 μg/ml SFE most likely due to its diminution of platelet derived growth factor (PDGF)-BB secreted by neighbor macrophages. HAoSMC migration was also enhanced by culturing in THP-1 macrophage conditioned media, as evidenced by a scratch wound assay. However, the presence of ≥10 μg/ml SFE did not allow such migaration. When SFE was treated to THP-1 macrophages, the secretion of the adipokines, visfatin and resistin, was abrogated. SFE at 1—25 μg/ml dose-dependently diminished resistin-stimulated secretion of collagen IV and connective tissue growth factor (CTGF) in HAoSMC, indicating that macrophage resistin plays a role in the extracellular matrix (ECM) production of vascular SMC. These results demonstrate that SFE disturbed proliferation and migration of SMC instigated by inflammatory macrophages in close proximity. Therefore, this study provides novel information that SFE has the potential capability to prevent atherosclerosis involving SMC proliferation, migration and fibrogenic activation within the vessels.</P>

등골나물 추출물이 인간의 유방암세포인 MDA-MB-231 세포의 이동, 침윤 및 부착에 미치는 영향

우은영(Eun Young Woo),박소영(So Young Park),권수진(Soo Jin Kwon),권규택(Gyoo Taik Kwon),김종대(Jong Dae Kim),임순성(Soon Sung Lim),윤정한(Jung H.Y. Park) 한국식품과학회 2011 한국식품과학회지 Vol.43 No.2

등골나물은 국화과 여러해살이 식물로 한방에서는 고혈압, 폐렴, 황달, 홍역, 요통 등에 사용한다고 알려져 있다. 본 연구에서는 등골나물의 꽃 부위를 추출하여 등골나물 추출물이 유방암 세포인 MDA-MB-231 세포의 이동, 침윤 및 부착에 미치는 영향을 조사하였다. 그 결과 MDA-MB-231 세포의 이동, 침윤 및 부착은 등골나물 추출물의 농도(0-20 μg/mL)가 증가할수록 현저하게 감소하였다. 등골나물 추출물은 MMP-9, MMP-2의 활성을 억제하였고, TIMP-1의 발현은 감소시킨 반면 TIMP-2의 발현은 증가시켰다. 또한, 등골나물 추출물은 uPA, VEGF 그리고 ICAM의 mRNA 및 단백질 수준을 현저히 감소시켰다. 특히, 등골나물 헥산 분획물이 유방암세포의 이동을 현저하게 억제하였다. 이상의 결과로부터 등골나물 추출물은 MMP-9, MMP-2, uPA, TIMP-1 및 ICAM의 감소, TIPM-2의 증가를 통해 유방암세포의 전이를 억제하는 것으로 판단된다. 따라서 본 연구는 이러한 효능을 지닌 등골나물 추출물을 암전이에 효과가 있는 암예방제나 항암제로 개발할 수 있는 가능성을 제시한다. The metastatic effect of Eupatorium japonicum extract (EJE) on MDA-MB-231 human breast cancer cells was investigated. MDA-MB-231 cells were treated with various concentrations of EJE (0, 5, 10 and 20 μg/mL). EJE inhibited cell migration, invasion and adhesion of MDA-MB-231 cells in dose-dependent manners. Gelatin zymography exhibited that EJE significantly down regulated secretion of matrix metalloproteinase (MMP)-9 and MMP-2. EJE decreased the protein levels of tissue inhibitor of metalloproteinase (TIMP)-1 but increased TIMP-2 levels. Additionally, EJE reduced the protein and mRNA levels of urokinase-type plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule (ICAM). In several solvent fractions of EJE, the hexane fraction markedly decreased MDAMB-231 cell migration. Thus, these finding suggest that EJE may be a potential antimetastatic agent, which can considerably inhibit the metastatic and invasive capacity of breast cancer cells.

Bone marrow-derived, alternatively activated macrophages enhance solid tumor growth and lung metastasis of mammary carcinoma cells in a Balb/C mouse orthotopic model

Cho, Han Jin,Jung, Jae In,Lim, Do Young,Kwon, Gyoo Taik,Her, Song,Park, Jong Hoon,Park, Jung Han Yoon BioMed Central 2012 Breast cancer research Vol.14 No.3

<P><B>Introduction</B></P><P>Tumor-associated macrophages, which are derived from the infiltration of circulating bone marrow-derived monocytes, consist primarily of a polarized M2 macrophage (M2-Mϕ) population and are associated with poor prognosis in various cancers. In the present study, we attempted to assess whether M2-Mϕs derived from bone marrow stimulate the promotion and progression of mammary tumors.</P><P><B>Methods</B></P><P>4T1 murine mammary carcinoma cells were injected either alone or coupled with M2-Mϕs into the mammary fat pads of syngeneic female Balb/C mice. M2-Mϕs were prepared by treating monocytes isolated from female Balb/C mouse bone marrow with IL-4. Tumor cell growth was determined using an <I>in vivo </I>imaging system and the expression of cell proliferation-related, angiogenesis-related, and lymphangiogenesis-related proteins in tumor tissues was immunohistochemically analyzed. To evaluate the effects of the crosstalk between 4T1 cells and M2-Mϕs on the secretion and mRNA expression of cytokines and the migration of monocytes, 4T1 cells and M2-Mϕs were co-cultured and cytokine antibody array, real-time RT-PCR, and trans-well migration assays were conducted.</P><P><B>Results</B></P><P>The co-injection of M2-Mϕs into the mammary fat pads of mice increased solid tumor growth and lung metastasis of 4T1 cells as well as the infiltration of CD45<SUP>+ </SUP>leukocytes into tumor tissues. The proportions of Ki-67<SUP>+ </SUP>proliferating cells and the expression of hypoxia inducible factor-1α, vascular endothelial cell growth factor A, CD31, vascular endothelial cell growth factor C, and lymphatic vessel endothelial receptor-1 were increased significantly in the tumor tissues of mice co-injected with 4T1 cells and M2-Mϕs. The <I>in vitro </I>results revealed that the proliferation of 4T1 cells, the migration of monocytes, and the secretion of granulocyte colony-stimulating factor, IFNγ, IL-1α, IL-2, IL-16, IFNγ-induced protein-10, keratinocyte-derived chemokine, macrophage colony-stimulating factor, monocyte chemotactic protein-1, macrophage inflammatory protein-1α, and RANTES were increased when 4T1 cells were co-cultured with M2-Mϕs, as compared with when the 4T1 cells were cultured alone.</P><P><B>Conclusion</B></P><P>The crosstalk between 4T1 cells and M2-Mϕs increased the production of cytokines, which may have induced immune cell infiltration into tumor tissues, tumor cell proliferation, angiogenesis, and lymph angiogenesis, thereby increasing solid tumor growth and lung metastasis.</P>

Anti-inflammatory and anti-allergic effects of Black raspberry (Rubus occidentalis) extract in RAW 264.7 and RBL-2H3 cells

Hye Ran Choi,Su Jung Lee,Sung Woong Gim,Gyoo Taik Kwon,Beob Won Kim,Kyung-Tae Lee,Daekeun Shin 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Effects of Pre-matured Black Raspberry on Benign Prostatic Hyperplasia (in vitro & in vivo) using a Rat Model

Su Jung Lee,Hye Ran Choi,Sung woong Gim,Gyoo Taik Kwon 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

청시닥나무 수피 에탄올 추출물의 항염증 효과

이한나(Han Na Lee),김진규(Jin Kyu Kim),권규택(Gyoo Taik Kwon),심재훈(Jae-Hoon Shim),김종대(Jong Dai Kim),윤정한(Jung Han Yoon) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.9

본 연구를 통하여 청시닥나무의 에탄올 추출물은 쥐 대식 세포인 Raw264.7 세포에 LPS로 유도된 염증반응에 미치는 효과가 있음을 확인하였다. 청시닥나무 목질부와 수피부에 에탄올을 가하여 추출한 뒤 그 추출물과 분획물의 NO 생성능 및 세포증식능을 실험한 결과 수피부의 EtOAc 분획이 세포증식능에 영향을 주지 않으면서 NO의 생성을 억제함을 확인하였다. 청시닥나무 수피부 에탄올 추출물 EtOAc 분획(EFEBA)은 Raw264.7 세포에서 LPS에 의해 생성된 NO의 분비와 iNOS의 단백질 및 mRNA의 발현을 농도 의존적으로 감소시켰고, 염증 반응 시 생성되는 IL-6, IL-1β 그리고 TNF-α의 mRNA의 발현도 현저히 감소시켰다. 또한 IκBα의 degradation을 감소시키고 p65의 인산화를 감소시켜 NF-κB signaling을 통해 염증작용을 조절함을 확인하였다. Acer barbinerve Maxim belongs to the Aceraceae tree family and is often consumed as an Oriental medicine. In this study, we investigated whether or not ethanol extract from the bark of A. barbinerve Max. (EBA) inhibits lipopolysaccharide (LPS)-induced inflammatory responses in Raw264.7 macrophages. EBA was fractionated using n-hexane, CH2Cl2, ethyl acetate (EtOAc), and water. Raw264.7 cells were treated with 20 μg/mL of EBA and the EBA fractions. EBA inhibited LPS-induced nitric oxide (NO) production. Among the three fractions, EtOAc fraction of EBA (EFEBA) was the most effective in inhibiting LPS-induced NO production without significant cytotoxicity in Raw264.7 cells. EFEBA futher reduced LPS-induced expression of inducible NO synthase (iNOS) proteins and its corresponding mRNA. Additionally, EFEBA decreased the mRNA levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in LPS-treated Raw264.7 cells. Lastly, EFEBA inhibited LPS-induced degradation of the inhibitor of kappaBalpha (IκBα) as well as phosphorylation of p65 nuclear factor-κB (NF-κB). These results indicate that EFEBA exhibits strong anti-inflammatory effects and can be developed as a potential anti-inflammatory agent.