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송후근,김정용,Guthold Martin 한국물리학회 2012 새물리 Vol.62 No.1
Deep Ultraviolet (DUV) fluorescence is very useful in imaging biological tissues and cells. It uses their intrinsic auto-fluorescence without fluorescence dying or tagging and without the need to determine the band-edge photoluminescences of wide bandgap nanomaterials. We successfully constructed a DUV fluorescence microscope operating in the 280 nm wavelength range by replacing and arranging the UV-transmitting optics and the UV LED. The results of the performance test of the constructed DUV fluorescence microscope showed that it can be conveniently used to obtain the epi-fluorescence image of electrospun fibrinogen nanofibers and thermally grown ZnO nanowires. 원자외선(Deep Ultra Violet: DUV) 형광 현미경은 생체조직의 자연형광을이용하여 형광표식 없이 형광이미징을 구현할 수 있으며, 광대역에너지갭 반도체 물질의 에피 형광 이미징을 수행할 수 있는 유용한 분석방법이다. 우리는 자외선에 대한 투과성이 낮은 보통 형광현미경 내부의광학계를 UV 투과성이 우수한 UV 광학계로 교체, 적절히 배치하고, UV LED를 광원으로 사용함으로써 280 nm 파장대에서 작동하는 DUV 형광현미경을 성공적으로 제작하였다. 제작된 DUV 형광현미경으로트립토판 아미노산, ZnO 나노선, fibrinogen 나노섬유의 DUV 형광이미징을 성공적으로 수행하였다.
Kim, Jeongyong,Song, Hugeun,Park, Inho,Carlisle, Christine R.,Bonin, Keith,Guthold, Martin Wiley Subscription Services, Inc., A Wiley Company 2011 Microscopy research and technique Vol.74 No.3
<P><B>Abstract</B></P><P>Deep ultraviolet (DUV) microscopy is a fluorescence microscopy technique to image unlabeled proteins via the native fluorescence of some of their amino acids. We constructed a DUV fluorescence microscope, capable of 280 nm wavelength excitation by modifying an inverted optical microscope. Moreover, we integrated a nanomanipulator‐controlled micropipette into this instrument for precise delivery of picoliter amounts of fluid to selected regions of the sample. In proof‐of‐principle experiments, we used this instrument to study, in situ, the effect of a denaturing agent on the autofluorescence intensity of single, unlabeled, electrospun fibrinogen nanofibers. Autofluorescence emission from the nanofibers was excited at 280 nm and detected at ∼350 nm. A denaturant solution was discretely applied to small, select sections of the nanofibers and a clear local reduction in autofluorescence intensity was observed. This reduction is attributed to the dissolution of the fibers and the unfolding of proteins in the fibers. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc.</P>
Simple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy
Neupane, Guru P.,Dhakal, Krishna P.,Kim, Min Su,Lee, Hyunsoo,Guthold, Martin,Joseph, Vincent S.,Hong, Jong-Dal,Kim, Jeongyong SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.5