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Gustavo Guevara-Balcazar,Israel Ramirez-Sanchez,Elvia Mera-Jimenez,Ivan Rubio-Gayosso,Maria Eugenia Aguilar-Najera,Maria C. Castillo-Hernandez 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.13 No.1
Vascular reactivity can be influenced by the vascular region, animal age, and pathologies present. Prostaglandins (produced by COX-1 and COX-2) play an important role in the contractile response to phenylephrine in the abdominal aorta of young rats. Although these COXs are found in many tissues, their distribution and role in vascular reactivity are not clear. At a vascular level, they take part in the homeostasis functions involved in many physiological and pathologic processes (e.g., arterial pressure and inflammatory processes). The aim of this study was to analyze changes in the contractile response to phenylephrine of thoracic/abdominal aorta and the coronary artery during aging in rats. Three groups of rats were formed and sacrificed at three distinct ages: prepubescent, young and old adult. The results suggest that there is a higher participation of prostanoids in the contractile effect of phenylephrine in pre-pubescent rats, and a lower participation of the same in old rats. Contrarily, there seems to be a higher participation of prostanoids in the contractile response of the coronary artery of older than pre-pubescent rats. Considering that the changes in the expression of COX-2 were similar for the three age groups and the two tissues tested, and that expression of COX-1 is apparently greater in older rats, COX-1 and COX-2 may lose functionality in relation to their corresponding receptors during aging in rats.
Gustavo Guevara-Balcazar,Israel Ramirez-Sanchez,Elvia Mera-Jimenez,Ivan Rubio-Gayosso,Maria Eugenia Aguilar-Najera,Maria C. Castillo-Hernandez 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.4
Vascular reactivity can be influenced by the vascular region, animal age, and pathologies present. Prostaglandins (produced by COX-1 and COX-2) play an important role in the contractile response to phenylephrine in the abdominal aorta of young rats. Although these COXs are found in many tissues, their distribution and role in vascular reactivity are not clear. At a vascular level, they take part in the homeostasis functions involved in many physiological and pathologic processes (e.g., arterial pressure and inflammatory processes). The aim of this study was to analyze changes in the contractile response to phenylephrine of thoracic/abdominal aorta and the coronary artery during aging in rats. Three groups of rats were formed and sacrificed at three distinct ages: prepubescent, young and old adult. The results suggest that there is a higher participation of prostanoids in the contractile effect of phenylephrine in pre-pubescent rats, and a lower participation of the same in old rats. Contrarily, there seems to be a higher participation of prostanoids in the contractile response of the coronary artery of older than pre-pubescent rats. Considering that the changes in the expression of COX-2 were similar for the three age groups and the two tissues tested, and that expression of COX-1 is apparently greater in older rats, COX-1 and COX-2 may lose functionality in relation to their corresponding receptors during aging in rats.
Guevara-Balcazar, Gustavo,Ramirez-Sanchez, Israel,Mera-Jimenez, Elvia,Rubio-Gayosso, Ivan,Aguilar-Najera, Maria Eugenia,Castillo-Hernandez, Maria C. The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.4
Vascular reactivity can be influenced by the vascular region, animal age, and pathologies present. Prostaglandins (produced by COX-1 and COX-2) play an important role in the contractile response to phenylephrine in the abdominal aorta of young rats. Although these COXs are found in many tissues, their distribution and role in vascular reactivity are not clear. At a vascular level, they take part in the homeostasis functions involved in many physiological and pathologic processes (e.g., arterial pressure and inflammatory processes). The aim of this study was to analyze changes in the contractile response to phenylephrine of thoracic/abdominal aorta and the coronary artery during aging in rats. Three groups of rats were formed and sacrificed at three distinct ages: prepubescent, young and old adult. The results suggest that there is a higher participation of prostanoids in the contractile effect of phenylephrine in pre-pubescent rats, and a lower participation of the same in old rats. Contrarily, there seems to be a higher participation of prostanoids in the contractile response of the coronary artery of older than pre-pubescent rats. Considering that the changes in the expression of COX-2 were similar for the three age groups and the two tissues tested, and that expression of COX-1 is apparently greater in older rats, COX-1 and COX-2 may lose functionality in relation to their corresponding receptors during aging in rats.
Taxonomic Reassessment of the Genus Padina (Dictyotales, Phaeophyta) from the Gulf of California
Riosmena-Rodriguez, Rafael,Paul-Chavez, Litzia,Hernandez-Carmona, Gustavo,Lopez-Vivas, Juan Manuel,Casas-Valdez, Margarita The Korean Society of Phycology 2009 ALGAE Vol.24 No.4
A monographic reassessment of Padina Adanson species from the Gulf of California was developed based on an exhaustive review of the character and characters states that have been used to delimit species in recent and historically valuable collections. Eight names (P. caulescens Thivy in Taylor, P. concrescens Thivy in Taylor, P. crispata Thivy in Taylor, P. durvillaei Bory Saint-Vincent, P. gymnospora [Kutzing] Sonder, P. mexicana Dawson, P. tetrastromatica Hauck and P. vickersiae Hoyt in Howe) were reported from the region. An analysis of 1,200 specimens led to the conclusion that only three species (P. durvillaei, P. concrescens and P. mexicana) can be recognized for the area based on four discriminating characters: presence of calcium carbonate in the cell wall; number of medullary cell layers in the basal area; number of medullary cell layers in the middle part and presence/absence of cortical cells. Comparative analysis of the species in the Gulf of California in relation to other recently evaluated species has shown a clear distinction among them suggesting Padina species were overestimated in our area.
Dietary Biotin Supplementation Impairs Testis Morphology and Sperm Quality
Karina Pasten-Hidalgo,Leticia Riveron-Negrete,Gloria Sicilia-Argumedo,Gustavo Canul-Medina,Tonatiuh Salazar-Anzures,Miguel Tapia-Rodrıguez,Enrique O. Hernandez-Gonzalez,Ana Lilia Roa-Espitia,Carlos Ce 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.5
Supplements containing pharmacological concentrations of biotin are commercially available over the counter. Classical toxicity studies have considered biotin administration as harmless; however, recent investigations have shown that biotin supplementation modifies tissue morphology without changes in toxicity markers, raising concerns about the consequences of morphological changes on tissues' functions and the safety of pharmacological concentrations of the vitamin. Testes are very sensitive to toxicants, and testicular histology is a reliable method to study its function. In this work, we investigated the effects of dietary biotin supplementation on testis morphology and spermatogenesis function using an experimental model, in which we have not observed unfavorable effects on other tissue functions or toxicity markers. Male BALB/cAnNHsd mice were fed a control or a biotin-supplemented diet (1.76 or 97.7 mg biotin/kg diet) for 8 weeks. Compared to the control group, the biotin-supplemented mice presented remarkable testis morphology changes, including increased spermatogonia layers; the cellular mechanism involved is related to increased proliferation. Sperm count and serum testosterone levels were not affected, but spermatozoa motility and morphology were significantly impaired in the biotin-supplemented mice. These results caution against the use of supplements with high concentrations of biotin and indicate that biotin's pharmacological effects on morphology need to be considered in toxicological studies.
Parada, Gloria M.,Riosmena-Rodriguez, Rafael,Martinez, Enrique A.,Hernandez-Carmona, Gustavo The Korean Society of Phycology 2012 ALGAE Vol.27 No.2
Water motion is one of the main drivers in morphological variability in species within de order Laminariales, and most of our current knowledge is based on subtidal populations. $Eisenia$ $arborea$ is a dominant kelp species in the North Pacific, widely distributed along the Baja California Peninsula from mid intertidal down to subtidal areas. This species presents great variability in the intertidal zone but it has not been yet evaluated such variability according to wave exposure. The present work also identifies the spatial / temporal variation, particularly respect to the presence of stipes without medulla (hollow stipes) a feature common among other brown seaweeds. We evaluated the effects of wave action in morphological variation of intertidal $Eisenia$ $arborea$ (Laminariales, Ochrophyta) at Punta Eugenia. The spatial and temporal variation sampling was surveyed between February, May, July, and August 2004 in the intertidal of Punta Eugenia, Baja California Sur. Our results have shown that exposed sites correlate with increased length and width of stipes as compared to more protected sites. Hollow stipes frequency changed more in association with temporal variation than with spatial heterogeneity suggesting nutrient limitation for thalli development. Our results suggest that $Eisenia$ $arborea$ compensate by morphological modifications the stress of living in the intertidal zone by showing larger stipes. Hollow stipes might be are also a mechanical adaptation to increase survival in high energy environments.
Ramos-Guerrero, Anelsy,Gonzalez-Estrada, Ramses Ramon,Hanako-Rosas, Greta,Bautista-Banos, Silvia,Acevedo-Hernandez, Gustavo,Tiznado-Hernandez, Martin Ernesto,Gutierrez-Martinez, Porfirio 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3
Soursop (Annona muricata) is a tropical fruit that can be infected by Colletotrichum gloeosporioides and Rhizopus stolonifer. Traditional methods used for postharvest disease control include the application of fungicides, however due to their excessive use, as well as their persistence in the environment, the development of new strategies that control pathogens are required. The application of chitosan (Chi), salicylic acid (SA) and methyl jasmonate (MJ) is an environmentally-friendly alternative with antimicrobial properties and also induces defense mechanisms in plant tissues. In this study, Colletotrichum was reactivated and Rhizopus was identified using morphological features and molecular tools. In vitro, the application of 0.5 and 1.0% of Chi alone or in combination with SA and MJ decreased mycelial growth and sporulation, a complete inhibition of spore germination was obtained. Thus, the application of Chi in combination with SA and MJ could be a smart strategy to inhibit the development of pathogens that attack soursop fruit.