Reduced Expression of Limd1 in Ulcerative Oral Epithelium Associated with Tobacco and Areca Nut

Maiti, Guru Prasad,Ghosh, Amlan,Chatterjee, Ramdas,Roy, Anup,Sharp, Tyson V.,Roychoudhury, Susanta,Panda, Chinmay Kumar Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Purpose: The aim of this study was to cast light on initiating molecular events associated with the development of premalignant oral lesions induced by tobacco and/or areca nut. Method: Immunohistochemical analyses of cell cycle regulatory proteins (LIMD1, RBSP3, p16, RB, phosphorylated RB, p53), EGFR and SH3GL2 (EGFR associated protein) were performed with inflammatory/ulcerative epithelium and adjacent hyperplastic/mild dysplastic lesions. Results: No change in expression of the proteins was seen in inflammatory epithelium. Reduced nuclear expression of LIMD1 was evident in ulcerative epithelium. In hyperplastic lesions, reduced expression of RBSP3, p16, SH3GL2 and overexpression of p-RB and EGFR were apparent. Reduced nuclear expression of p53 was observed in mild dysplastic lesions. Conclusion: Our data suggest that inactivation of LIMD1 in ulcerative epithelium might predispose the tissues to alterations of other cell cycle regulatory and EGFR signaling proteins needed for the development of premalignant oral lesions.

SENSITIZATION OF PERIODONTITIS DISEASE CAUSING BACTERIA BY LOW POWER He-Ne LASER RADIATION

Akash Tripathi Satsangi,Guru Prasad 한국광과학회 2004 Journal of Photosciences Vol.11 No.2

Reduction of Proliferation and Induction of Apoptosis are Associated with Shrinkage of Head and Neck Squamous Cell Carcinoma due to Neoadjuvant Chemotherapy

Sarkar, Shreya,Maiti, Guru Prasad,Jha, Jayesh,Biswas, Jaydip,Roy, Anup,Roychoudhury, Susanta,Sharp, Tyson,Panda, Chinmay Kumar Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Neoadjuvant chemotherapy (NACT) is a treatment modality whereby chemotherapy is used as the initial treatment of HNSCC in patients presenting with advanced cancer that cannot be treated by other means. It leads to shrinkage of tumours to an operable size without significant compromise to essential oro-facial organs of the patients. The molecular mechanisms behind shrinkage due to NACT is not well elucidated. Materials and Methods: Eleven pairs of primary HNSCCs and adjacent normal epithelium, before and after chemotherapy were screened for cell proliferation and apoptosis. This was followed by immunohistochemical analysis of some cell cycle (LIMD1, RBSP3, CDC25A, CCND1, cMYC, RB, pRB), DNA repair (MLH1, p53) and apoptosis (BAX, BCL2) associated proteins in the same set of samples. Results: Significant decrease in proliferation index and increase in apoptotic index was observed in post-therapy tumors compared to pre-therapy. Increase in the RB/pRB ratio, along with higher expression of RBSP3 and LIMD1 and lower expression of cMYC were observed in post-therapy tumours, while CCND1 and CDC25A remained unchanged. While MLH1 remained unchanged, p53 showed higher expression in post-therapy tumors, indicating inhibition of cell proliferation and induction of apoptosis. Increase in the BAX/BCL2 ratio was observed in post-therapy tumours, indicating up-regulation of apoptosis in response to therapy. Conclusions: Thus, modulation of the G1/S cell cycle regulatory proteins and apoptosis associated proteins might play an important role in tumour shrinkage due to NACT.

Modeling sulfuric acid induced swell in carbonate clays using artificial neural networks

Sivapullaiah, P.V.,Guru Prasad, B.,Allam, M.M. Techno-Press 2009 Geomechanics & engineering Vol.1 No.4

The paper employs a feed forward neural network with back-propagation algorithm for modeling time dependent swell in clays containing carbonate in the presence of sulfuric acid. The oedometer swell percent is estimated at a nominal surcharge pressure of 6.25 kPa to develop 612 data sets for modeling. The input parameters used in the network include time, sulfuric acid concentration, carbonate percentage, and liquid limit. Among the total data sets, 280 (46%) were assigned to training, 175 (29%) for testing and the remaining 157 data sets (25%) were relegated to cross validation. The network was programmed to process this information and predict the percent swell at any time, knowing the variable involved. The study demonstrates that it is possible to develop a general BPNN model that can predict time dependent swell with relatively high accuracy with observed data ($R^2$=0.9986). The obtained results are also compared with generated non-linear regression model.

Inner-Gate-Engineered GAA MOSFET to Enhance the Electrostatic Integrity

Biswajit Jena,Sidhartha Dash,Soumya Ranjan Routray,Guru Prasad Mishra 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.10

Gate-all-around (GAA) MOSFETs are the best multi-gate MOSFET structure due to their strong electrostatic control over the channel. The electrostatic controllability can be enhanced further by applying some gate engineering technique to the existing GAA structure. This paper investigates the effect of inner gate (core gate) on the electrostatic performance of conventional GAA MOSFET. The inner gate engineering increases both the electrostatic control and packing density of GAA MOSFET. In this paper, we have presented an inner-gate-engineered (IGE) GAA MOSFET and inspected its advantages over conventional counterparts. The proposed structure exhibits higher I on/I off ratio, low threshold voltage and improved RF performances as compared to the conventional structure. Analytic simulation has been carried out for numerous figures of merit (FOMs) for different technology nodes.

Dielectrically Modulated Hetero Channel Double Gate MOSFET as a Label Free Biosensor

Soumya S. Mohanty,Sikha Mishra,Meryleen Mohapatra,Guru Prasad Mishra 한국전기전자재료학회 2022 Transactions on Electrical and Electronic Material Vol.23 No.2

A dielectric modulation (DM) highly sensitive InP/InGaAs/InP hetero channel dual material double gate MOSFET (H-DMDG MOSFET) is designed and simulated for accurate identifi cation of the bio-targets such as protein, streptavidin, uricase, biotin, APTES, and Keratin. Group III–V based materials are considered for biosensor design for its chemical inertness, high temperature/power ability, and high carrier velocity. Nanogap cavity is incorporated near the source and drain ends within the gate dielectric for sensing the neutral and charged analytes. The simulated parameters of the proposed structure are analyzed by varying dielectric constant and the charge density of the biomolecules. The infl uence of both the biomolecules on the electrical features like surface potential, drain current, electric fi eld, and sensitivity have been examined. The highest sensitivity of 0.513 and a lower electric fi eld of 1.98 × 10 6 V/cm is attained at the drain end for K = 8 as compared to other biomolecules.

Incidental Detection of Ischemic Myocardium on 68 Ga-FAPI PET/CT

Chandra Piyush,Nath Satish,Krishnamoorthy Jaishankar,Sogunuru Guru Prasad 대한핵의학회 2021 핵의학 분자영상 Vol.55 No.4

Recent studies using Ga-68-labeled fibroblast activation protein inhibitors (FAPI) PET have shown strong association between focal uptake of FAPI in myocardium and presence of coronary artery disease. We present an interesting case of a 76-year-old female with breast cancer with incidental uptake on FAPI PET in apex and septal wall of left ventricle myocardium correlating with findings of ischemia on dobutamine stress myocardial perfusion imaging and anatomical stenosis on coronary angiography.

Sensitization of Periodontitis Disease Causing Bacteria by Low Power He-Ne Laser Radiation

Satsangi, Akash Tripathi,Mathur, Manish,Saxena, Parul,Prasad, Guru,Shrivastava, Jitendra Nath,Shrivastava, Jenendra Nath,Roy, Sukhdev Korean Society of Photoscience 2004 Journal of Photosciences Vol.11 No.2

The present investigation is an attempt to create an optimized protocol for a bactericidal modality of different powers of He-Ne laser radiation to eliminate periodontitis disease causing bacteria from dental plaques. Periodontitis is most prevalent infectious disease of men and caused by a limited number of Gram negative oral bacteria. Porphyromonas gingivalis and Streptococcus sanguis are the important bacteria responsible for periodontitis diseases. Effect on periodontitis disease causing bacteria were produced by the exposure of different powers of He-Ne laser light i.e. 9 mW, 17 mW and 26 mW of red colour of wavelength 632.8 nm in two different periods of time i.e. 10 min. and 20 min. in the presence of dye Methylene blue (MB) used as a photosensitizer. The results have been shown in terms of percentage inhibition of colony forming units (cfu.) of bacteria. This study has shown that maximum inhibition of cfu. were observed in Laser+MB-20 min. exposure time. This inhibition was followed by Laser+MB-10 min., but minimum inhibition was seen in Laser only at 10 min. exposure. In case of effect of methylene alone on the cfu. of bacteria, it was seen that MB have not shown more inhibition of cfu. and it had shown that the no. of cfu. are very similar to that of control. The above observation of the present study was seen in case of every 3 different type of used powers of laser for both the bacteria. Maximum percentage inhibition of cfu. were seen in case of 26mW powers of He-Ne laser, which was 67. 28% to 61.42% for Porphyromonas gingivalis and Streptococcus sanguis respectively. So, increasing the power of laser (safe range for dentistry is 3-30 mW) under conditions shows an increased percentage inhibition of cfu. Thus the present investigation may be a useful adjunct with mechanical debridement in the prevention of recolonization of subgingival lesions by pathogenic microorganisms which are harmful and drug resistant.

Comparison of Various Joint Decompression Techniques in Septic Arthritis of the Hip in Children: A Systematic Review and Meta-Analysis

( Sitanshu Barik ),( Vikash Raj ),( Sant Guru Prasad ),( Richa ),( Varun Garg ),( Vivek Singh ) 대한고관절학회 2023 Hip and Pelvis Vol.35 No.2

The aim of this review is to conduct an analysis of existing literature on outcomes of application of various methods of joint decompression in management of septic arthritis of the hip in children. A search of literature in PubMed, Embase, and Google Scholar was conducted for identification of studies reporting on the outcomes of intervention for septic arthritis of the hip in children. Of the 17 articles selected, four were comparative studies; two of these were randomized controlled trials while the rest were single arm studies. Statistical difference was observed between the proportion of excellent clinical and radiological outcomes in arthrotomy (90%, 95% confidence interval [CI] 81-98%; 89%, 95% CI 80-98%), arthroscopy (95%, 95% CI 91-100%; 95%, 95% CI 90- 99%), and arthrocentesis (98%, 95% CI 97-100%; 99%, 95% CI 97-100%), respectively. The highest overall rate of additional unplanned procedures was observed in the arthrocentesis group (24/207, 11.6%). Patients who underwent arthrocentesis had a statistically greater chance of excellent clinical and radiological outcomes, although the highest level of need for additional unplanned surgical intervention was observed in the arthrocentesis group, followed by the arthroscopy group and the arthrotomy group. Future conduct of a prospective multicentric study focusing on the developed and developing world, along with acquisition of data. such as delay of treatment and severity of disease will enable assessment of the efficacy of one technique over the other by surgeons worldwide.

Felodipine loaded PLGA nanoparticles: preparation, physicochemical characterization and in vivo toxicity study

Jana Utpal,Mohanty Anjan Kumar,Pal Sovan Lal,Manna Prabal Kumar,Mohanta Guru Prasad 나노기술연구협의회 2014 Nano Convergence Vol.1 No.31

Felodipine, a calcium channel blocker has been widely used for the treatment of hypertension and cardiovascular diseases; but the frequent dosing is needed for its poor solubility and variable bioavailability. In present study an attempt has been made to overcome the problems through nanoparticulate delivery system using poly (D, L-lactic-co-glycolic acid) polymer keeping in the view to get better sustainability of the formulation. The nanoparticles were prepared by single emulsion solvent evaporation technique and the physico-chemical characterization of prepared nanoparticles confirmed the particles were nanosize range with smooth and spherical morphology. Further, the compatibility of drug-polymer combination was analyzed by FTIR and DSC study. The in vitro drug release study of PLGA nanoparticles showed longer duration of drug release with reduced burst release compared with pure felodipine. The in vitro drug release data were fitted with various mathematical models to establish the drug release mechanism from the nanoparticles and found to follow mixed order kinetics. The in vivo toxicity study in albino mice showed no noticeable change in biochemical parameters and histopathology of organs. Hence, the developed felodipine nanoparticles were prepared, characterized and could possibly be advantageous for prolonged drug release and improving the antihypertensive effect.