The Effect of Hydrogen on Blood Glucose Metabolism and High-density Lipoprotein Function in Patients with Type 2 diabetes

Guohua Song 한국물학회 2016 한국물학회지 Vol.5 No.2

Molecular Hydrogen Stabilizes Atherosclerotic Plaque in Low-density Lipoprotein Receptor Knockout Mice

Guohua Song 한국물학회 2015 한국물학회지 Vol.4 No.2

Objective Hydrogen (H2) attenuates the development of atherosclerosis in mouse models. We aimed to examine the effects of H2 on atherosclerotic plaque stability. Methods Low-density lipoprotein receptor knockout (LDLR-/-) mice fed an atherogenic-diet were dosed daily with H2 and/or simvastatin. In vitro studies were carried out in oxidized-LDL (ox-LDL)-stimulated macrophage-derived foam cell model treated with or without H2. Key Results H2 or simvastatin significantly enhanced plaque stability by increasing levels of collagen and smooth muscle cells, as well as reducing macrophage and lipid levels in plaques. The decreased numbers of dendritic cells and increased numbers of regulatory T cells in plaques further supported the stabilizing effect of H2 or simvastatin. Moreover, H2 treatment decreased serum ox-LDL level and apoptosis in plaques with concomitant inhibition of endoplasmic reticulum stress (ERS) and reduction of ROS accumulation in the aorta. In vitro, like ERS inhibitor 4-phenylbutyric acid, H2 inhibited ox-LDL- or tunicamycin (an ERS inducer)- induced ERS response and cell apoptosis. In addition, like ROS scavenger N-acetyl-cysteine, H2 inhibited ox-LDL- or Cu2 + (an ROS inducer)-induced reduction in cell viability and increase in cellular ROS. Also, H2 increased Nrf2 (NF-E2-related factor-2, an important factor in antioxidant signaling) activation and Nrf2 siRNA abolished the protective effect of H2 on ox-LDL-induced cellular ROS production. Conclusions The inhibitory effects of H2 on the apoptosis of macrophage-derived foam cells, which take effect by suppressing the activation of ERS pathway and by activating Nrf2 antioxidant pathway, might lead to an improvement in atherosclerotic plaque stability.

A Serial study on the effect of hydrogen administration on lipoprotein metabolism and atherosclerotic plaque formaton

Guohua Song,Chuanlong Zong,Hua Tian,Hui Sang,Yang Yu,Yazhuo Xue,Shucun Qin 한국물학회 2014 한국물학회 학술대회 Vol.2014 No.11

Effect of Hydrogen and Oxygen Mixed Inhalation on Non-alcoholic Fatty Liver Disease

Xue Yazhuo,Zhang Guangjie,Song Guohua,Qin Shucun 한국물학회 2019 한국물학회지 Vol.7 No.1

Non-alcoholic fatty liver disease is closely related to insulin resistance and genetic susceptibility, and it is a metabolic stress liver injury. As a result of the multifactorial pathological development in vivo, endogenous reactive oxygen species (ROS) increase, leading to inflammatory necrosis of hepatocytes. In recent years, many studies have confirmed that hydrogen is not a physiological inert gas. It has reliable therapeutic properties for some diseases, such as type 2 diabetes, Parkinson's disease, atherosclerosis and so on. It can be said that hydrogen is a gas with great potential medical value. We used nitrogen-oxygen mixture as control, and inhaled hydrogen-oxygen mixture (66% hydrogen and 33% oxygen) to non-alcoholic fatty liver patients. We found that hydrogen can improve the blood lipid level, reduce the plasma total cholesterol level and low density lipoprotein level, and increase the liver/spleen CT ratio in patients with non-alcoholic fatty liver. At the same time, hydrogen can improve the anti-inflammatory and anti-oxygen ability of plasma in patients with non-alcoholic fatty liver. It can reduce plasma glutamic oxaloacetic transaminase and alanine transaminase levels in patients with nonalcoholic fatty liver disease. The results showed that hydrogen could protect hepatocytes and alleviate the degree of nonalcoholic fatty liver.

Degradation Kinetics of Anthocyanins from Purple Sweet Potato (Ipomoea batatas L.) as Affected by Ascorbic Acid

Jing Li,Huige Song,Nan Dong,Guohua Zhao 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1

Storage (4oC and 25oC, 28 days) and thermal(70oC-90oC, 6 h) stabilities of purple sweet potatoanthocyanins (PSPAs) with varying concentrations ofascorbic acid (AA) were investigated in a model soft drinkmedium. For storage stability, the model drink wassterilized at 85oC for 15 min prior to storage. Zero-orderkinetics and first-order kinetics were fitted for storagedegradation at 4oC and 25oC, respectively. However, alldata for thermal degradation fitted first-order kinetics. Thetemperature dependence on degradation was modeled afterthe Arrhenius equation. Storage degradation of PSPAs wasincreased by the presence of AA (40-360 mg/L). Retardedthermal degradation was be achieved by adding 120 mg/Lof AA, while accelerated thermal degradation resultedfrom 360 mg/L of AA. Heat treatment did not markedlychange the DPPH radical-scavenging activity of PSPAs.

Higher Serum C Reactive Protein Determined C Reactive Protein Single-Nucleotide Polymorphisms Are Involved in Inherited Depression

Shiliang Wang,Hua Zhong,Meijuan Lu,Guohua Song,Xiaomei Zhang,Min Lin,Shengliang Yang,Mincai Qian 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.8

Objective_The pathogenesis of depression is not fully understood yet, but studies have suggested higher circulating C reactive protein (CRP) level might relate to depression occurrence. However, due to high variability of patients’ individual condition, the results to date are inconsistent. Considering CRP single-nucleotide polymorphisms (SNPs) could also regulate plasma CRP levels, in the present study, we hypothesized that inherited CRP allelic variations may co-vary with depressive symptomatology. Methods_We recruited 60 depression patients with family depression history and 60 healthy control volunteers into this project. We detected circulation CRP level as well as genome CRP SNPs from participants of this project. Results_We have found a significantly higher circulating CRP level in patients with a positive family history. Furthermore, we also identified some certain inherited CRP SNPs (A allele in rs1417938 and C allele in rs1205) could up regulate serum CRP level and distributed more in depression patients with family history. Conclusion_Our finding may raise new evidence that genetically increased serum CRP level through SNPs variation is likely to induce family inherited depression.

Genome-wide identification of auxin-responsive microRNAs in the poplar stem

Yang Lihua,Ping Tao,Lu Wenjin,Song Sangfa,Wang Jianli,Wang Qiao,Chai Guohua,Bai Yue,Chen Yan 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background Wood (secondary xylem) of forests is a material of great economic importance. Wood development is strictly controlled by both the phytohormone auxin and microRNAs (miRNAs). Currently, the regulatory mechanisms underlying wood formation by auxin-associated miRNAs remain unclear. Objective This report was designed to identify auxin-responsive miRNAs during wood formation. Methods Morphological observation of wood development in the poplar stems was performed under the treatment of different concentrations (0 mg/L, CK; 5 mg/L, Low; 10 mg/L, High) of indol-3-butyric acid (IBA). Using a small RNA sequencing strategy, the effect of IBA treatment on miRNAs expression was genome-widely analyzed. Results In this study, we found that wood development of poplar was promoted by low concentration of IBA treatment but inhibited by high concentration of IBA treatment. Stringent bioinformatic analysis led to identification of 118 known and 134 novel miRNAs candidates. Sixty-nine unique developmental-related miRNAs, corresponding to 269 target genes, exhibited specific expression patterns in response to auxin, as was consistent with the influence of auxin application on wood formation. Three novel miRNAs had the most number (≥ 9) of target genes, belonging to SPL, GRF and ARF gene families. The evolutionary relationships and tissue expression patterns of 41 SPL, GRF and ARF genes in poplar were thus analyzed. Of them, four representative members and corresponding miRNAs were confirmed using RT-qPCR. Conclusions Our results may be helpful for a better understanding of auxin-induced regulation of wood formation in tree species.