Effects of Acupuncture at Zu-San-Li (ST36) on the Activity of the HypothalamicePituitaryeAdrenal Axis during Ethanol Withdrawal in Rats

Zheng Lin Zhao,Bong Hyo Lee,Feng Lin,YanQin Guo,Yi Yan Wu,Sang Mi Park,Sunghyeon In,Sang Chan Kim,Chae Ha Yang,Rong Jie Zhao 사단법인약침학회 2014 Journal of Acupuncture & Meridian Studies Vol.7 No.5

The current study investigated the effects of acupuncture at Zu-San-Li (ST36) on the hypothalamicepituitaryeadrenalaxis during ethanol withdrawal in rats. Rats were intraperitoneallytreated with 3 g/kg/day of ethanol or saline for 28 days. Following 24 hours ofethanol withdrawal, acupuncture was applied at bilateral ST36 points or non-acupoints(tail) for 1 minute. Plasma levels of corticosterone (CORT) and adrenocorticotropic hormone(ACTH) were measured by radioimmunoassay (RIA), and the corticotropin-releasingfactor (CRF) protein levels in the paraventricular nucleus of the hypothalamus were alsoexamined by RIA 20 minutes after the acupuncture treatment. RIA showed significantlyincreased plasma levels of CORT and ACTH in the ethanol-withdrawn rats compared withthe saline-treated rats, which were inhibited significantly by the acupuncture at the acupointST36 but not at the non-acupoint. Additionally, ethanol withdrawal promoted CRFprotein expressions in the paraventricular nucleus of the hypothalamus, which were alsoblocked by the acupuncture at ST36. These findings suggest that acupuncture at the specific acupoint ST36 can inhibit ethanol withdrawal-induced hyperactivation of hypothalamicepituitaryeadrenalaxis, and it may be mediated via the modulation of hypothalamicCRF.

Expression of Transcription Factor FOXC2 in Cervical Cancer and Effects of Silencing on Cervical Cancer Cell Proliferation

Zheng, Chun-Hua,Quan, Yuan,Li, Yi-Yang,Deng, Wei-Guo,Shao, Wen-Jing,Fu, Yan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Objective: Forkhead box C2 (FOXC2) is a member of the winged helix/forkhead box (Fox) family of transcription factors. It has been suggested to regulate tumor vasculature, growth, invasion and metastasis, although it has not been studied in cervical cancer. Here, we analyzed FOXC2 expression in cervical tissues corresponding to different stages of cervical cancer development and examined its correlation with clinicopathological characteristics. In addition, we examined the effects of targeting FOXC2 on the biological behavior of human cervical cancer cells. Methods: The expression of FOXC2 in normal human cervix, CIN I-III and cervical cancer was examined by immunohistochemistry and compared among the three groups and between cervical cancers with different pathological subtypes. Endogenous expression of FOXC2 was transiently knocked down in human Hela and SiHa cervical cells by siRNA, and cell viability and migration were examined by scratch and CCK8 assays, respectively. Results: In normal cervical tissue the frequency of positive staining was 25% (10/40 cases), with a staining intensity (PI) of $0.297{\pm}0.520$, in CIN was 65% (26/40cases), with a PI of $3.00{\pm}3.29$, and in cancer was 91.8% (68/74 cases), with a PI of $5.568 {\pm}3.449$. The frequency was 100% in adenocarcinoma (5/5 cases) and 91.3% in SCCs (63/69 cases). The FOXC2 positive expression rate was 88.5% in patients with cervical SCC stage I and 100% in stage II, showing significant differences compared with normal cervix and CIN. With age, pathologic differentiation degree and tumor size, FOXC2 expression showed no significant variation. On transient transfection of Hela and SiHa cells, FOXC2-siRNA inhibition rates were 76.2% and 75.7%; CCK8 results showed reduced proliferation and relative migration (in Hela cells from $64.5{\pm}3.16$ to $49.5{\pm}9.24$ and in SiHa cells from $60.1{\pm}3.05$ to $44.3{\pm}3.98$) (P < 0.05). Conclusion: FOXC2 gene expression increases with malignancy, especially with blood vessel hyperplasia and invasion degree. Targeted silencing was associated with reduced cell proliferation as well as invasion potential.

A Distributed Face Recognition Framework Based on Data Fusion

Zheng Zhang,Yan Guo,Guozhi Song 보안공학연구지원센터 2014 International Journal of Database Theory and Appli Vol.7 No.4

Active Compounds from Schisandra chinensis Exhibiting Tyrosinase Activity and Melanin Content Inhibition in B16 Melanoma Cells

Zheng-Fei Yan,Jian Guo,Feng-Hua Tian,Xin-Xin Mao,Yu Li,Chang-Tian Li 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

Schisandra chinensis has been used as traditional medicine. The structures of isolate active compounds (schisandrin B, deoxyschisandrin, schisandrin C) from S. chinensis were characterized by physical and spectroscopic analyses. Active compounds were tested for their potential to act as anti-melanogenesis or skin-whitening agents by their abilities to inhibit tyrosinase activity in the cell-free mushroom tyrosinase assay and cellular tyrosinase derived from B16 melanoma cells. The tyrosinase inhibitory activity was correlated to the inhibition of melanin productions in α-MSH-stimulated and unstimulated B16 cells. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver- Burk plot. Schisandrin B was minimally cytotoxic (cell viability: 88.99% at 0.75 μM) and the IC50 value for suppression of mushroom tyrosinase activity was estimated as 0.6 μM. Zymography analysis demonstrated schisandrin B’s concentration-dependent effects and the kinetic analysis indicated schisandrin B’s noncompetitive-inhibitory action.

Bithiophene-Imide-Based Polymeric Semiconductors for Field-Effect Transistors: Synthesis, Structure−Property Correlations, Charge Carrier Polarity, and Device Stability

Guo, Xugang,Ortiz, Rocio Ponce,Zheng, Yan,Hu, Yan,Noh, Yong-Young,Baeg, Kang-Jun,Facchetti, Antonio,Marks, Tobin J. American Chemical Society 2011 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.133 No.5

Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng

Ze-Yan Fan,Cui-Ping Miao,Xin-Guo Qiao,You-Kun Zheng,Hua-Hong Chen,You-Wei Chen,Li-Hua Xu,Li-Xing Zhao,Hui-Lin Guan 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2

Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.

Fretting Fatigue Behavior of Ti–6Al–4V and Ti–10V–2Fe–3Al Alloys

Zhi Yan Li,Xiao Long Liu,Guo Qing Wu,Zheng Huang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.1

The effect of fretting on fatigue performance of different microstructures for titanium alloy was studied using a high-frequencypush–pull fatigue testing machine. Both plain and fretting fatigue curves were obtained for comparative analysis of the frettingeffect on fatigue performance of the different titanium alloy. The result shows that the strength, plain fatigue of Ti6Al4Vtitanium is lower than those of Ti1023 titanium. But the fretting fatigue of Ti6Al4V titanium is higher under each contactstress. The fatigue source depth of Ti1023 alloy is greater than Ti6Al4V alloy. Hardening of Ti1023 alloy is more seriousafter fretting. The wear mechanism of two titanium alloys is different, Ti1023 titanium alloy is more sensitive to fretting wear.

Nonsingular Fast Terminal Sliding Mode Control Strategy for PMLSM Based on Disturbance Compensation

Li Zheng,Zhang Zi-Hao,Wang Jin-Song,Yan Zhi-Bang,Guo Xiao-Qiang,Sun He-Xu 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3

To optimize the anti-interference performance and dynamic response ability of permanent magnet linear synchronous motor (PMLSM) and enhance the overall control performance of the system, the novel nonsingular fast terminal sliding mode controller (NFTSMC) based on disturbance compensation is proposed in this design. To enhance the response speed and stability of the controller, this design introduces the system state variable into the exponential reaching law to make the controller adaptively adjust with the change of the system state, which can reduce the chattering of the system and have a faster response speed. The extended sliding mode disturbance observer is designed to estimate the interference factors in the system, and the controller is feedforward compensated to further improve the anti-interference ability of the PMLSM system. Meanwhile, the control strategy is proved by the Lyapunov stability theory. Simulation and experiments show that the proposed control strategy has better dynamic response ability and anti-interference ability than the traditional NFTSMC.

Response Surface Optimized Extraction of Flavonoids from Mimenghua and Its Antioxidant Activities In vitro

Lei Guo,Wen-Cheng Zhu,Ya-Ting Liu,Jiu-Yu Wu,An-Qi Zheng,Yan-Li Liu 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.5

The optimized extraction conditions of total flavonoids from mimenghua were determined by the Box-Behnken design and response surface methodology. The optimal conditions to achieve the maximum yield of flavonoids were determined as follows: ethanol concentration 68%(v/v), extraction temperature 72.4oC, liquid/material ratio 48:1mL/g, and extraction time 2 h. Under the optimized conditions, the extraction ratio of flavonoids was 15.44%. The crude extract was then purified by AB-8 macroporous adsorption resin, the flavonoids content in the purified extract increased to 90.43%. The antioxidant activities of the purified flavonoids were evaluated in vitro by scavenging capabilities of DPPH radical, hydroxyl radical and superoxide radical, reducing power and total antioxidant capacity. The results showed that the flavonoids have significant antioxidant activities, which can be used as a source of potential antioxidant.

Luciferase Assay to Screen Tumour-specific Promoters in Lung Cancer

Xu, Rong,Guo, Long-Jiang,Xin, Jun,Li, Wen-Mao,Gao, Yan,Zheng, You-Xian,Guo, You-Hong,Lin, Yang-Jun,Xie, Yong-Hua,Wu, Ya-Qing,Xu, Rui-An Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Objective: Specific promoters could improve efficiency and ensure the safety of gene therapy. The aim of our study was to screen examples for lung cancer. Methods: The firefly luciferase gene was used as a reporter, and promoters based on serum markers of lung cancer were cloned. The activity and specificity of seven promoters, comprising CEACAM5 (carcinoembryonic antigen, CEA), GRP (Gastrin-Releasing Peptide), KRT19 (cytokeratin 19, KRT), SFTPB (surfactant protein B, SP-B), SERPINB3 (Squamous Cell Carcinoma Antigen, SCCA), SELP (Selectin P, Granule Membrane Protein 140kDa, Antigen CD62, GMP) and DKK1 (Dickkopf-1) promoters were compared in lung cancer cells to obtain cancer-specific examples with strong activity. Results: The CEACAM5, DKK1, GRP, SELP, KRT19, SERPINB3 and SFTPB promoters were cloned. Furthermore, we successfully constructed recombinant vector pGL-CEACAM5 (DKK1, GRP, SELP, KRT19, SERPINB3 and SFTPB) contained the target gene. After cells were transfectedwith recombinant plasmids, we found that the order of promoter activity from high to low was SERPINB3, DKK1, SFTPB, KRT19, CEACAM5, SELP and GRP and the order for promoters regarding specificity and high potential were SERPINB3, DKK1, SELP, SFTPB, CEACAM5, KRT19 and GRP. Conclusion: The approach adopted is feasible to screen for new tumour specific promoters with biomarkers. In addition, the screened lung-specific promoters might have potential for use in lung cancer targeted gene therapy research.