골담초의 이소후라본 성분

金光洙,李景道,安丙浚 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

Five isoflavones were isolated from the root of Caragana microphylla Lam. and the structures have been identified by means of chemical and physical methods. The isoflavones are 7-hydroxy-4'-methoxyisoflavone(formononetin), formononetin-7-O-β-glucoside(ononin), 7-hydroxy-3', 4'-methylenedioxyisoflavone(pseudobaptigenin), 7-O-β-glucuopyranosyloxy-3'-hydroxy-4'-methoxyi-soflavone(calycosin-7-O-β-gluc-oside) and maackiain.

한국인 및 중국 한족 정신분열병 환자의 5-HT2A 수용체 유전자 -1438A/G 다형성

이장호,이광철,이승부,오용인,최영근,조아랑,정주호,장환일 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

Objectives : The purpose of the present study was to investigate the association between -1438A/G polymorphism of 5-HT2A receptor gene and schizophrenia in Korean and Han Chinese population. Methods : A sample of 184 Korean patients with schizophrenia and 96 Korean healthy normal controls and 96 Han Chinese patients with schizophrenia and 96 Han-Chinese healthy normal controls were genotyped for a single nucleotide polymorphism with in 5-HT2A receptor gene (promoter region, A-1438G) by Msp I Resthction Fragment Length Polymorphism (RFLP). Results : There was no difference in allelic frequencies and genotype frequencies of -1438A/G polymorphism between Korean schizophrenics and controls (p=0.13) and Han Chinese schizophrenics and controls (p=0.40). Also, -1438A/G Poly-morphism did not show ethnical difference between Korean and Han Chinese controls. The Scale for the Assessment of Negative Symptoms (SANS) scores showed no significant differences between genotypes of -1438A/G polymorphism in both of Korean and Han Chinese schizophrenics. Conclusion : These results suggest that -1438A/G polymorphism of the 5-HT2A receptor gene is not causally related to the development of schizophrenia in Korean and Han Chinese population, and there no ethnic difference between Korean and Han Chinese population.

중국의 경제성장과 지역불균형

沈義燮,李光勳 명지대학교 리서치아카데미 2000 리서치 아카데미 논총 Vol.3 No.-

여성에서 왜 우울증이 많은가?

송지영,이광철 경희대학교 2002 慶熙醫學 Vol.18 No.3-4

TSSG growth, morphology and properties of potassium lithium niobate (KLN) crystals

Chong, Tow-Chong,Xu, Xue-Wu,Li, Lian,Zhang, Guang-Yu,Kumagai, H.,Hirano, M. The Korea Association of Crystal Growth 1999 한국결정성장학회지 Vol.9 No.4

In the present paper, potassium lithium niobate(KLN) crystals have been grown along <001>, <100> and <110> directions by the top seeded solution growth (TSSG) method from Li-richer melts with different compositions. The morphologies of KLN crystals grown along different directions have been studied, and the well-developed facets have been unambiguously indexed using X-ray goniometer and stereographic projection analysis. The growth mechanism and defects such as cracks and inclusions were discussed on the basis of observations of facets on the crystal-solution interfaces. The crystal compositions were determined by a chemical analysis method. The structure and lattice constants of KLN crystals were determined and calculated on the basis of XRD data by using TREOR90 and PIRUM programs. The Curie temperature and optical absorption were determined by dielectric constant peak and spectrum measurements. respectively. The blue second harmonic generation (SHG) characteristics of KLN sample were also investigated using a pulsed dye laser.

Sources, distribution, bioavailability, toxicity, and risk assessment of heavy metal(loid)s in complementary medicines

Bolan, Shiv,Kunhikrishnan, Anitha,Seshadri, Balaji,Choppala, Girish,Naidu, Ravi,Bolan, Nanthi S.,Ok, Yong Sik,Zhang, Ming,Li, Chun-Guang,Li, Feng,Noller, Barry,Kirkham, Mary Beth Elsevier 2017 Environment international Vol.108 No.-

<P><B>Abstract</B></P> <P>The last few decades have seen the rise of alternative medical approaches including the use of herbal supplements, natural products, and traditional medicines, which are collectively known as ‘Complementary medicines’. However, there are increasing concerns on the safety and health benefits of these medicines. One of the main hazards with the use of complementary medicines is the presence of heavy metal(loid)s such as arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg). This review deals with the characteristics of complementary medicines in terms of heavy metal(loid)s sources, distribution, bioavailability, toxicity, and human risk assessment. The heavy metal(loid)s in these medicines are derived from uptake by medicinal plants, cross-contamination during processing, and therapeutic input of metal(loid)s. This paper discusses the distribution of heavy metal(loid)s in these medicines, in terms of their nature, concentration, and speciation. The importance of determining bioavailability towards human health risk assessment was emphasized by the need to estimate daily intake of heavy metal(loid)s in complementary medicines. The review ends with selected case studies of heavy metal(loid) toxicity from complementary medicines with specific reference to As, Cd, Pb, and Hg. The future research opportunities mentioned in the conclusion of review will help researchers to explore new avenues, methodologies, and approaches to the issue of heavy metal(loid)s in complementary medicines, thereby generating new regulations and proposing fresh approach towards safe use of these medicines.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A first-time comprehensive overview on the health risk assessment of heavy metal(loid)s in complementary medicines </LI> <LI> Ayurvedic medicines contain toxic levels of heavy metal(loid)s including As, Cd, Hg and Pb </LI> <LI> The bioavailability of metal(loid)s in complementary medicines depends on speciation of these metals </LI> <LI> Regular intake of some complementary medicines has caused metal(loid) toxicity in humans </LI> <LI> Health risk assessment can be achieved based on the daily intake of complementary medicines and total metal(loid) content </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Interactions between heavy metal(loid)s and complementary medicines.</P> <P>[DISPLAY OMISSION]</P>

Disruption of endothelial barrier function is linked with hyposecretion and lymphocytic infiltration in salivary glands of Sjögren's syndrome

Cong, Xin,Zhang, Xue-Ming,Zhang, Yan,Wei, Tai,He, Qi-Hua,Zhang, Li-Wei,Hua, Hong,Lee, Sang-Woo,Park, Kyungpyo,Yu, Guang-Yan,Wu, Li-Ling Elsevier 2018 Biochimica et biophysica acta. Molecular basis of Vol.1864 No.10

<P><B>Abstract</B></P> <P>Sjögren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-γ stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endothelial tight junction barrier is disrupted in hyposecretory submandibular glands from Sjögren's syndrome mouse model </LI> <LI> The disrupted salivary endothelial barrier is linked with lymphocytic infiltration in Sjögren's syndrome mouse model </LI> <LI> The redistribution of claudin-5 is responsible for disrupted endothelial barrier in salivary glands from Sjögren's syndrome </LI> </UL> </P>

CMC and dynamic properties of poly(VA-b-St) copolymer micelles for drug delivery

Guang-Hua Li,Chang-Gi Cho 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6

The polymeric micelles from amphiphilic block copolymer poly(vinyl alcohol-b-styrene) (poly(VA-b-St)) with different syndiotacticity of poly(vinyl alcohol) (PVA) block were prepared by dialysis against water. Critical micelle concentration (CMC) and dynamic properties of poly(VA-b-St) copolymeric micelles were investigated by fluorescence techniques. From the fluorescence emission spectrum measurements using pyrene as a fluorescence probe, the observed CMC value was in the range of 0.125-4.47 mg/L. The CMC value increased with decreasing the weight ratio of PS to PVA block and with increasing the syndiotacticity of PVA block. The rate of pyrene release was very slow for block copolymers containing PVA block with higher syndiotacticity, which indicates that their micelles have increased kinetic stability.

cDNA cloning, expression, and immunolocalization of gonadinhibiting hormone (GIH) in Litopenaeus vannamei

Guang-li Li,Si-ping Deng,Shu-na Jiang,Man Ye,Hua-pu Chen,Siuming F. Chan,Chun-hua Zhu 한국유전학회 2015 Genes & Genomics Vol.37 No.10

In this study, the full-length GIH cDNA sequence from Litopenaeus vannamei was cloned from the eyestalk by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The fulllength GIH cDNA was 865 bp with a 288 bp open-reading frame, which encoded a 96 amino acid prepro-GIH with 17 amino acid signal peptide. L. vannamei GIH (LvGIH) can be classified as a member of type-II crustacean hyperglycemic hormone polypeptide family. LvGIH shares 93.8 and 66.7 % amino acid sequence identity with GIH from Penaeus monodon, and the molt-inhibiting hormone from Marsupenaeus japonicas, respectively. By quantitative real-time PCR (qPCR), LvGIH mRNA transcripts were detected in fertilized eggs, nauplius, zoea, mysis and juveniles of 25, 35 and 40 days old. LvGIH transcript levels increased significantly with the development from fertilized eggs to juveniles. LvGIH transcript levels were highest in juveniles at 35 days old. By RT-PCR, LvGIH mRNA transcripts were detected only in the eyestalks and brains but not in the muscles, intestines, gills, heart, hepatopancreas, ovaries and testes of adults, and there was no difference in the expression level of LvGIH between males and females. Using the P. monodon anti-GIH antibody, we showed that LvGIH was located mainly in the XO-SG and slightly in axon, with similar fluorescence intensity found in XO and SG. To summarize, we have cloned and characterized the GIH of the shrimp L. vannamei. In addition to the GIH properties described in other crustaceans, a peak of LvGIH expression was identified at the time of sexual differentiation (i.e., day 35 larvae) suggesting that LvGIH may also be involved in the control of this process.

Adaptive Multiple MPC for a Wind Farm with DFIG: a Decentralized-Coordinated Approach

LI, Xiao-ming,ZHANG, Xi,LIN, Zhong-wei,NIU, Yu-guang,LI, Ming-yang,Nathan, Noel Vishal The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.5