http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Magnetic Nanoparticles for Early Detection of Cancer by Magnetic Resonance Imaging
Lin, Wenbin,Hyeon, Taeghwan,Lanza, Gregory M.,Zhang, Miqin,Meade, Thomas J. Cambridge University Press (Materials Research Soc 2009 MRS bulletin Vol.34 No.6
<B>Abstract</B><P>This article provides a brief overview of recent progress in the synthesis and functionalization of magnetic nanoparticles and their applications in the early detection of malignant tumors by magnetic resonance imaging (MRI). The intrinsic low sensitivity of MRI necessitates the use of large quantities of exogenous contrast agents in many imaging studies. Magnetic nanoparticles have recently emerged as highly efficient MRI contrast agents because these nanometer-scale materials can carry high payloads while maintaining the ability to move through physiological systems. Superparamagnetic ferrite nanoparticles (such as iron oxide) provide excellent negative contrast enhancement. Recent refinement of synthetic methodologies has led to ferrite nanoparticles with narrow size distributions and high crystallinity. Target-specific tumor imaging becomes possible through functionalization of ferrite nanoparticles with targeting agents to allow for site-specific accumulation. Nanoparticulate contrast agents capable of positive contrast enhancement have recently been developed in order to overcome the drawbacks of negative contrast enhancement afforded by ferrite nanoparticles. These newly developed magnetic nanoparticles have the potential to enable physicians to diagnose cancer at the earliest stage possible and thus can have an enormous impact on more effective cancer treatment.</P>
장영주,주희재,양정인,서창원,정귀예,Gregory M. Lanza,Huiying Zhang 한국통합생물학회 2011 Animal cells and systems Vol.15 No.4
This study was undertaken to produce a F_(ab) fragment of a human monoclonal antibody reactive to oxidized and carbamylated low-density lipoprotein (oxLDL and cLDL) using phage display technology. An analysis of DNA sequences of this F_(ab), termed plaque 15,16-46 F_(ab), revealed that the rearranged VH was highly mutated. Complementarity-determining regions of the VH showed a very high R/S ratio and contained many positively charged amino acids. In direct binding and competitive ELISA, the F_(ab) reacted strongly with both MDA-LDL and Cu-oxLDL forms of oxLDL, and also showed high affinity for cLDL. Immunofluorescence and immunohistochemical analyses showed that this F_(ab) positively stained atherosclerotic aortic plaques in ApoE/ mice as well as those in patients with atherosclerosis. The F_(ab) also showed positive staining in placental decidua from patients with preeclampsia. It is suggested that the plaque 15,16-46 F_(ab) against oxLDL and cLDL might possibly be applicable for developing a diagnostic reagent for both human and rodent animal research to detect and characterize atherosclerotic disease progression in atherosclerotic lesions as well as exploring the pathogenesis of atherogenic diseases such as preeclampsia.
Jang, Young-Ju,Joo, Hee-Jae,Yang, Jeong-In,Seo, Chang-Won,Chung, Kui-Yea,Lanza, Gregory M.,Zhang, Huiying The Korean Society for Integrative Biology 2011 Animal cells and systems Vol.15 No.4
This study was undertaken to produce a $F_{ab}$ fragment of a human monoclonal antibody reactive to oxidized and carbamylated low-density lipoprotein (oxLDL and cLDL) using phage display technology. An analysis of DNA sequences of this $F_{ab}$, termed plaque 15,16-46 $F_{ab}$, revealed that the rearranged $V_H$ was highly mutated. Complementarity-determining regions of the $V_H$ showed a very high R/S ratio and contained many positively charged amino acids. In direct binding and competitive ELISA, the $F_{ab}$ reacted strongly with both MDA-LDL and Cu-oxLDL forms of oxLDL, and also showed high affinity for cLDL. Immunofluorescence and immunohistochemical analyses showed that this $F_{ab}$ positively stained atherosclerotic aortic plaques in $ApoE^{-/-}$ mice as well as those in patients with atherosclerosis. The $F_{ab}$ also showed positive staining in placental decidua from patients with preeclampsia. It is suggested that the plaque 15,16-46 $F_{ab}$ against oxLDL and cLDL might possibly be applicable for developing a diagnostic reagent for both human and rodent animal research to detect and characterize atherosclerotic disease progression in atherosclerotic lesions as well as exploring the pathogenesis of atherogenic diseases such as preeclampsia.