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- 저자 : Gen-jin Liu (검색결과 4 건)
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Gen-Xia Liu,Shu Ma,Yao Li,Yan Yu,Yi-Xiang Zhou,Ya-Die Lu,Lin Jin,Zi-Lu Wang,Jin-Hua Yu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
The putative tumor suppressor microRNA let-7c is extensively associated with the biological properties of cancer cells. However, the potential involvement of let-7c in the differentiation of mesenchymal stem cells has not been fully explored. In this study, we investigated the influence of hsa-let-7c (let-7c) on the proliferation and differentiation of human dental pulp-derived mesenchymal stem cells (DPMSCs) treated with insulin-like growth factor 1 (IGF-1) via flow cytometry, CCK-8 assays, alizarin red staining, real-time RT-PCR, and western blotting. In general, the proliferative capabilities and cell viability of DPMSCs were not significantly affected by the overexpression or deletion of let-7c. However, overexpression of let-7c significantly inhibited the expression of IGF-1 receptor (IGF-1R) and downregulated the osteo/odontogenic differentiation of DPMSCs, as indicated by decreased levels of several osteo/odontogenic markers (osteocalcin, osterix, runt-related transcription factor 2, dentin sialophosphoprotein, dentin sialoprotein, alkaline phosphatase, type 1 collagen, and dentin matrix protein 1) in IGF-1-treated DPMSCs. Inversely, deletion of let- 7c resulted in increased IGF-1R levels and enhanced osteo/odontogenic differentiation. Furthermore, the ERK, JNK, and P38 MAPK pathways were significantly inhibited following the overexpression of let-7c in DPMSCs. Deletion of let-7c promoted the activation of the JNK and P38 MAPK pathways. Our cumulative findings indicate that Let-7c can inhibit the osteo/odontogenic differentiation of IGF-1-treated DPMSCs by targeting IGF-1R via the JNK/P38 MAPK signaling pathways.
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Perforation optimization of hydraulic fracturing of oil and gas well
Zhu, Hai Yan,Deng, Jin Gen,Chen, Zi Jian,An, Feng Chen,Liu, Shu Jie,Peng, Cheng Yong,Wen, Min,Dong, Guang Techno-Press 2013 Geomechanics & engineering Vol.5 No.5
Considering the influences of fluid penetration, casing, excavation processes of wellbore and perforation tunnels, the seepage-deformation finite element model of oil and gas well coupled with perforating technique is established using the tensile strength failure criterion, in which the user-defined subroutine is developed to investigate the dynamic evolvement of the reservoir porosity and permeability. The results show that the increases of perforation angle and decreases of perforation density lead to a higher fracture initiation pressure, while the changes of the perforation diameter and length have no evident influences on the fracture initiation pressure. As for initiation location for the fracture in wellbore, it is on the wellbore face while considering the presence of the casing. By contrast, the fractures firstly initiate on the root of the tunnels without considering casing. Besides, the initial fracture position is also related with the perforation angle. The fracture initiation position is located in the point far away from the wellbore face, when the perforation angle is around $30^{\circ}$; however, when the perforation angle is increased to $45^{\circ}$, a plane fracture is initiated from the wellbore face in the maximum horizontal stress direction; no fractures was found around perforation tunnels, when the angel is close to $90^{\circ}$. The results have been successfully applied in an oilfield, with the error of only 1.1% comparing the fracture initiation pressure simulated with the one from on-site experiment.
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( Guo Hong Gong ),( Zhi Ming Zheng ),( Hui Liu,Li Wang ),( Jin Shan Diao ),( Peng Wang ),( Gen Hai Zhao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.6
An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.
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Bond-slip Constitutive Relation between BFRP Bar and Basalt Fiber Recycled-aggregate Concrete
Wen-jing Meng,Hua-xin Liu,Gen-jin Liu,Xiang-qing Kong,Xue-zhi Wang 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.5
In order to investigate the bond stress-slip constitutive relation between BFRP bar and basalt fiber recycled-aggregate concrete, 81 central pullout specimens were tested. Basalt fiber volume percent, fiber length and concrete strength grade were considered to be experimental variables to obtain the bond stress-slip curves. The results showed that adding basalt fiber into recycled-aggregate concrete will reduce the bond stress between BFRP bar and recycled-aggregate concrete but enhance the ductility of bond property; the bond stress increases with the increasing of fiber length and concrete strength grade. The bond stress-slip constitutive model was proposed and the results showed that the model is good in effect of fitting. The constitutive model can reflect the bonding mechanism between BFRP bar and basalt fiber concrete well, it also can provide reference for theoretical analysis and engineering applications of the bond and anchor property between BFRP bars strengthened recycled concrete.
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