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Ying Zeng,Bin Jiang,Ouling Shi,Gaofen Quan,Salih Al-Ezzi,FuSheng Pan 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.4
Some alloying elements (Al, Er, Gd, Li, Mn, Sn, Y, Zn) were proved recently by calculations or experiments to improve theformability of Mg alloys, but ignoring their site preference in Mg crystals during the calculated process. A crystallographicmodel was built via fi rst principle calculations to predict the site preferences of these elements. Regularities between dopingelements and site preferences were summarized. Meanwhile, in the basis of the crystallographic model, a series of formulaswere deduced combining the diff raction law. It predicted that a crystal plane with abnormal XRD peak intensity of the Mgbasedsolid solutions, compared to that of the pure Mg, prefers to possess solute atoms. Thus, three single-phase solid solutionalloys were then prepared through an original In-situ Solution Treatment, and their XRD patterns were compared. Finally,the experiment further described the site preferences of these solute atoms in Mg crystal, verifying the calculation results.
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Guangdong Hu,Jing Wang,Hui Huang,Fusheng Quan,Jian Kang,Yongyan Wu,Yuanpeng Gao,Feng Su,Minghao Shao,Yong Zhang 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2
Transposons are widely used for geneticengineering in various model organisms. Recently, piggyBac(PB) has been developed as a transposable and efficientgene transfer tool in mammalian cells. In the present study,we developed three types of PB transposon systemscontaining a dual plasmid system (DPS), a single plasmidsystem (SPS), and a DNA-mRNA combined system (DRPS)and characterized their basic properties in HEK293 cells. The basic elements of the donor plasmid included aselectable-reporter gene expression cassette, two loxP sitesin the same orientation, a multiple cloning site, and twochicken β-globin insulator core elements. We further identifiedthe function of the selectable-reporter and examined PBintegration sites in the human genome. Moreover, wecompared the transposition efficacy and found that SPStransposed more efficiently, as compared to DPS; integrationinto the host genome was determined by measuring PBaseactivity. Results discovered the loss of PBase activity in theDRPS, indicating that this system is much more biologicallysafe, as compared to DPS and SPS. Finally, we employedthe DRPS to successfully perform a gene delivery intobovine mammary epithelial cells (BMECs). Taken together,the information from this study will improve the flexibilityof PB transposon systems and reduce the genotoxicity ofPBase in genetic engineering.
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