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RISS 인기검색어
- 검색키워드
- 저자 : Furong Peng (검색결과 3 건)
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Comparative Study of Two Common In Vitro Models for the Pancreatic Islet with MIN6
Chao Xinxin,Zhao Furong,Hu Jiawei,Yu Yanrong,Xie Renjian,Zhong Jianing,Huang Miao,Zeng Tai,Yang Hui,Luo Dan,Peng Weijie 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.1
BACKGROUND: Islet transplantation is currently considered the most promising method for treating insulin-dependent diabetes. The two most-studied artificial islets are alginate-encapsulated b cells or b cell spheroids. As three-dimensional (3D) models, both artificial islets have better insulin secretory functions and transplantation efficiencies than cells in twodimensional (2D) monolayer culture. However, the effects of these two methods have not been compared yet. Therefore, in this study, cells from the mouse islet b cell line Min6 were constructed as scaffold-free spheroids or alginate-encapsulated dispersed cells. METHODS: MIN6 cell spheroids were prepared by using Agarose-base microwell arrays. The insulin secretion level was determined by mouse insulin ELISA kit, and the gene and protein expression status of the MIN6 were performed by Quantitative polymerase chain reaction and immunoblot, respectively. RESULTS: Both 3D cultures effectively promoted the proliferation and glucose-stimulated insulin release (GSIS) of MIN6 cells compared to 2D adherent cells. Furthermore, 1% alginate-encapsulated MIN6 cells demonstrated more significant effects than the spheroids. In general, three pancreatic genes were expressed at higher levels in response to the 3D culture than to the 2D culture, and pancreatic/duodenal homeobox-1 (PDX1) expression was higher in the cells encapsulated in 1% alginate than that in the spheroids. A western blot analysis showed that 1% alginate-encapsulated MIN6 cells activated the phosphoinositide 3-kinase (PI3K)/serine/threonine protein kinase (AKT)/forkhead transcription factor FKHR (FoxO1) pathway more than the spheroids, 0.5% alginate-, or 2% alginate-encapsulated cells did. The 3D MIN6 culture, therefore, showed improved effects compared to the 2D culture, and the 1% alginate-encapsulated MIN6 cells exhibited better effects than the spheroids. The upregulation of PDX1 expression through the activation of the PI3K/AKT/FoxO1 pathway may mediate the improved cell proliferation and GSIS in 1% alginate-encapsulated MIN6 cells. CONCLUSION: This study may contribute to the construction of in vitro culture systems for pancreatic islets to meet clinical requirements.
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A Novel Algorithm for Tracking and Forecasting Convective Cells Using Satellite Image Sequences
Jia Liu,Chuancai Liu,Chao Ma,Danyu Qin,Furong Peng 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2
Accurate storm tracking and forecasting are essential parts of severe weather warning operations. The main problem of existing tracking and forecasting algorithms is unphysical split and merger of cloud clusters within the life cycle of Mesoscale Convective System (MCS). To address this issue, an automatic algorithm called TFCC (Tracking and Forecasting Convective Cells) is proposed for tracking and forecasting convective cells using infrared (IR) image sequences from geostationary meteorology satellite. In this paper, convective cells are utilized for tracking and forecasting instead of MCS because convective cells are stable portion in MCS. TFCC algorithm utilizes overlapping technique and uses a dynamic constraint technique based combinatorial optimization method. Moreover, displacement of the geometrical centroid is utilized to forecast the movement of convective cells. Case studies show that convective cells are tracked and forecasted efficiently in different phases of MCS lifecycle including genesis, maturity and dissipation using TFCC algorithm. Categorical statistics and contingency tables method applied to various case studies over China show that TFCC algorithm efficiently and accurately.
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GRP78 Secreted by Colon Cancer Cells Facilitates Cell Proliferation via PI3K/Akt Signaling
Fu, Rong,Yang, Peng,Wu, Hai-Li,Li, Zong-Wei,Li, Zhuo-Yu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Glucose regulated protein 78 (GRP78) is usually recognized as a chaperone in the endoplasmic reticulum. However, increasing evidence indicates that GRP78 can be translocated to the cell surface, acting as a signaling receptor for a variety of ligands. Since little is known about the secretion of GRP78 and its role in the progression of colon cancer we here focused on GRP78 from colon cancer cells, and purified GRP78 protein mimicking the secreted GRP78 was able to utilize cell surface GRP78 as its receptor, activating downstream PI3K/Akt and Wnt/${\beta}$-catenin signaling and promote colon cancer cell proliferation. Our study revealed a new mode of action of autocrine GRP78 in cancer progression: secreted GRP78 binds to cell surface GRP78 as its receptor and activates intracellular proliferation signaling.
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