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Miao-miao Luo,Lu-jing Ren,Sheng-lan Chen,Xiao-jun Ji,He Huang 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.6
Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.
A Pediatric Oncology Diverse Learning Course on Caring for nursing students’ reflection
Miao Hsing Chen,Shu-Chuan Chang,Yu-Ping Huang,Yueh Chih Chen 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10
Aim(s): This study aimed to determine if a pediatric oncology diverse learning course could enhance nursing students’ theoretical knowledge and understanding of clinical care for children with leukemia and their families. Method(s): This was a qualitative retrospective study. Data were collected in July 2016. Participants were college nursing students (N = 33) enrolled in a diverse learning course in pediatric oncology, which was taught between November 2014 and January 2015 at a science and technology university in Taiwan. The three-part course included reading picture books, participating in role-playing, and watching a cancer-related family movie. Focus group discussions occurred during each part. Students maintained written reflections during the course, which were submitted to an e-learning website. Reflections were analyzed using content analysis. Result(s): Students’ reflections resulted in five main themes: (1) Pediatric oncology nursing knowledge and skills learned; (2) Understanding children with leukemia and their families suffering; (3) Empathy parents’ and children’s reactions to the effects of leukemia; (4) Nursing skills necessary for caring for children with leukemia; and (5) Expect future role as a pediatric oncology nurse. Conclusion(s): The pediatric oncology simulation course employed multiple teaching strategies. The improvements in student cognition, empathy, and understanding of care requirements of children with leukemia and emotional needs of families as pediatric oncology nurses suggest the teaching objectives of enhancing student learning were achieved. The knowledge and skills acquired could assist students in developing professional competencies and values in nursing.
Studies on Axial Tensile Loading Capacity of CHS T-joints Reinforced with External Stiffening Rings
Shanshan Huang,Lei Zhu,Hailin Sun,Miao Wang,Xi Chen 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.3
To analyze the eff ects of stiff ening external rings on the brace axial tension capacity of circular hollow section T-joints (CHST), loading experiments and fi nite element model analysis were utilized. Three groups of full-scale specimens (reinforced and unreinforced T-joints) of diff erent brace-to-chord diameter ratios were tested under tension to validate the strengthening eff ect (Three unreinforced specimens were previously tested and the other three reinforced samples were newly completed). The experimental equipment and parameters are described in detail. Load–ovalization and load–displacement curves in addition to the failure modes were analyzed. It is concluded that the ultimate strength and initial stiff ness are signifi cantly enhanced for the reinforced CHS joints compared to the unreinforced specimens. SHELL181 elements were utilized in the fi nite element analysis to accurately simulate the loading capacity with and without external stiff ening rings of the joints, within 10% error.
Li, Yan,Miao, Li-Yun,Xiao, Yong-Long,Huang, Mei,Yu, Min,Meng, Kui,Cai, Hou-Rong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Although associations between thioredoxin interacting protein (TXNIP) and cancers have been recognized, the effects of TXNIP on non-small cell lung cancer (NSCLC) prognosis remained to be determined in detail. In addition, while hypoxia is a key characteristic of tumor cell growth microenvironment, the effect of hypoxia on TXNIP expression is controversial. In this study, formaldehyde fixed and paraffin embedded (FFPE) samples of 70 NSCLC patients who underwent resection between January 2010 and December 2011 were obtained. Evaluation of TXNIP and hypoxia inducible factor-$1{\alpha}$ ($HIF-1{\alpha}$) protein expression in FFPE samples was made by immunohistochemistry. By Kaplan-Meier method, patients with high TXNIP expression demonstrated a significantly shorter progression free survival (PFS) compared with those with low TXNIP expression (18.0 months, 95%CI: 11.7, 24.3 versus 23.0 months, 95%CI: 17.6, 28.4, P=0.02). High TXNIP expression level was also identified as an independent prognostic factor by Cox regression analysis (adjusted hazard ratio: 2.46; 95%CI: 1.08, 5.56; P=0.03). Furthermore, TXNIP expression was found to be significantly correlated with $HIF-1{\alpha}$ expression (Spearman correlation=0.67, P=0.000). To further confirm correlations, we established a tumor cell hypoxic culture model. Expression of TXNIP was up-regulated in all three NSCLC cell lines (A549, SPC-A1, and H1299) under hypoxic conditions. This study suggests that hypoxia induces increased TXNIP expression in NSCLC and high TXNIP expression could be a poor prognostic marker.
Meng-Yuan Huang,Chi-Ming Yang,Man-Miao Yang,Wann-Neng Jane,Yung-Ta Chang 한국응용곤충학회 2009 Journal of Asia-Pacific Entomology Vol.12 No.3
It has been postulated that grana stacking and destacking are mediated by the alteration of the surface charge density on the thylakoid membrane, which is regulated by the LHCII phosphorylation and dephosphorylation cycle. Insect-induced cecidomyiid galls, transformed from Machilus thunbergii Sieb & Zucc. (Lauraceae) leaves, are totally deficient in the pigment–protein complexes CPI and A1 of PSI and AB1 and AB2 of PSII. Although the galls are deficient in LHCII complex, grana stacking is normal. Our data suggest that the LHCII complex may not be the only factor responsible for grana stacking of thylakoid membranes.
Liu Huan,Miao Yu,Tian Huayu,Chen Yishan,Wang Enfu,Huang Jingda,Zhang Wenbiao 한국탄소학회 2024 Carbon Letters Vol.34 No.3
Moso bamboo, as a kind of renewable functional material, exhibits outstanding development potential. It is promising to prepare activated carbon with good mechanical strength and high specific surface area using moso bamboo as raw material. In this work, we employed a hydraulic extruder to extrude the bamboo charcoal and the adhesive to obtain the moso bamboo activated carbon, and improved the specific surface area of the columnar activated carbon through high-temperature water vapor activation. Through the catalytic role of the water vapor activation process, the formation and expansion of the pores were promoted and the internal pores were greatly increased. The obtained columnar activated carbon shows excellent mechanical strength (93%) and high specific surface area (791.54 m2/g). Polyacrylamide@asphalt is one of the most effective adhesives in the high-temperature water vapor activation. The average pore size (22.99 nm) and pore volume (0.36 cm3/g) of the prepared columnar activated carbon showed a high mesoporous ratio (83%). Based on the excellent pore structure brought by the activation process, the adsorption capacity of iodine (1135.75 mg/g), methylene blue (230 mg/g) and carbon tetrachloride (64.03 mg/g) were greatly improved. The resultant moso bamboo columnar activated carbon with high specific surface area, excellent mechanical properties, and outstanding adsorption capacity possesses a wide range of industrial applications and environmental protection potential.