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RISS 인기검색어
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- 저자 : Fukang Gu (검색결과 3 건)
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Fei Yin,Fukang Gu,Xin Sheng,Quanxin Gao,Qili Li,Zhaohong Shi 한국통합생물학회 2013 Animal cells and systems Vol.17 No.4
Ciliature microtubular organelles, which organize into ciliary units, are highly complex microtubular systems. Toinvestigate the formation of ciliature microtubular organelles and the role that different tubulins play in this process,we examined the distribution and function of α-, γ-, and δ-tubulins in the hypotrich ciliate Stylonychia pustulata. Immunofluorescence analysis demonstrated that α-tubulin localized to all microtubular organelles, while γ- and δ-tubulinscolocalized at basal bodies. Immunogold labeling revealed that γ- and δ-tubulins colocalize to the proximalpart of basal bodies, suggesting that γ- and δ-tubulins probably interact and may function together. In addition, thepermanent localization of γ-tubulin to the adoral zone of membranelles suggests that γ-tubulin play a role in themaintenance of oral ciliature base-associated microtubules, but does not play a role in the biogenesis of theseciliature. We observe that δ-tubulin is restricted to the basal body and the connective fibers of the basal body,suggesting this protein may be required for the extension and stability of the microtubules in these structures. Dramatically different concentrations of these three tubulins were observed in the cytoskeleton and the insoluble andsoluble fractions of the cell, suggesting that these highly related proteins perform different functions in S. pustulata.
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Qili Li,Xinpeng Fan,Qianqian Sun,Na Wu,Bing Ni,Fukang Gu 한국통합생물학회 2017 Animal cells and systems Vol.21 No.1
Ciliates are able to form resting cysts as a survival strategy in response to stressful environmental factors. Studies on the characteristics of cellular structure during encystment may provide useful information for further understanding of the regulatory mechanism of cellular patterns and supply new clues regarding the phylogeny of ciliates. Scanning and transmission electron microscopies were used to observe the ultrastructure of cells during encystment of the soil ciliate Australocirrus cf. australis. The dedifferentiation of ciliature was revealed for the first time. Ciliary shafts first shortened, and the remaining ciliature, including basal bodies and the fibrillar cirral basket, retracted into the cytoplasm and was surrounded by the autophagic vacuoles and then gradually digested. A large number of autophagic vacuoles were observed in mature resting cysts. Autophagy might not only be necessary for the differentiation of cellular structures during encystment but might also be important to sustain the basic life activities in the resting stage. Australocirrus cf. australis formed a kinetosome-resorbing cyst and contained four layers in the cyst wall: the ectocyst, mesocyst, endocyst and granular layer. The ciliature resorbing state and the number of layers in the cyst wall were consistent with those found in other oxytrichous ciliates. However, the phenomenon wherein the two macronuclear nodules are not fused during encystment is not commonly observed among oxytrichids. Additionally, the octahedral granules in the mesocyst of this species exhibit different morphology from the congeners.
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Xiaocui Zhang,Xinpeng Fan,Linying Yang,Yiwen Wang,Bing Ni,Saleh A. Al-Farraj,Fukang Gu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.2
Extrusomes of different categories in ciliates have been suggested to have different structures and functions. To investigate the characteristics of extrusomes in the hypotrichous ciliate, we examined Architricha indicausing scanning and transmission electron microscopy and electron microscopic enzymo-cytochemistry. Theresults showed that the mature extrusomes were ellipsoidal vesicles with structureless cavities on their anteriorparts and arranged in rows beneath the pellicle; the extruded structures of extrusomes were cyathiform and canremain outside of the cell surface after extrusion. These findings differ distinctly from that of previouslyreported extrusomes in other ciliates and thus suggest a new type of extrusomes. These organelles originatedfrom endoplasmic reticulum in the deeper part of the cytoplasm, underwent a serious of development,gradually migrated to the cortical cytoplasm and finally positioned in their functional areas. In contrast withlower forms of ciliates, density, arrangement, and localization of extrusomes in A. indica showed obviousdifferences between the ventral and dorsal surface of cells. This may be in some way related to thedifferentiation of the dorsoventral cortex, and functional ciliature happened to the hypotrichous ciliates. Inaddition, the extrusomes may perform protective or defensive function, which associates with the activity ofmitochondria and contributes to the pellicle retrieval or renewal.
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