http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Francisco Javier Leyva-Jiménez,Jesús Lozano-Sánchez,Isabel Borrás-Linares,David Arráez-Román,Antonio Segura-Carretero 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.79 No.-
A manufacturing design and control of bioactive ingredients from Aloysia citriodora was performedapplying microwave-assisted extraction (MAE). Thus, a Response Surface Methodology based on CentralComposite Design 23 model was performed to monitor the matrix behavior. The factors were extractiontime, percentage of solvent and temperature. The chosen responses were extraction yield andphytochemicals recovery. The design allowed tofind the optimal conditions for each response and tovalidate them in order to attain high quality ingredients. These results pointed out that MAE is a noveltechnique which could be used to improve the new functional ingredient development.
Jose Luis Lozano Villanueva,Javier Francisco Torres Zafra,Fabián Cortés Muñoz,Fernán del Cristo Mendoza Beltrán,Jenny Carolina Sánchez Casas,Luis Alfonso Barragán Pedraza 대한신경과학회 2021 Journal of Clinical Neurology Vol.17 No.2
Background and Purpose Ischemic stroke is a common cause of death worldwide. In clinical practice it is observed that many individuals who have experienced an ischemic stroke also suffer from simultaneous comorbidities such as heart failure, which could be directly associated with a worse clinical prognosis. Therefore, this study analyzed outcomes in terms of the severity of the event, inhospital mortality, duration of hospital stay, and inhospital recurrence of the episode, in order to determine the implications resulting from the presentation of both pathologies. Methods This was a retrospective-cohort, hospital-based study. Results The study included 110 subjects with heart failure (exposed) and 109 subjects without heart failure (nonexposed). The incidence of inhospital mortality was 27.27% in exposed patients and 9.17% in nonexposed patients (p<0.001), and the presence of heart failure increased the risk of death by 92% (p=0.027). According to scores on the National Institutes of Health Stroke Scale, the median severity was worse in exposed than nonexposed patients (16.1 vs. 9.2, p=0.001). The median hospital stay was 9 days in subjects with heart failure and 7 days in nonexposed patients (p=0.011). The rate of inhospital stroke did not differ significantly between exposed and nonexposed patients (1.82% vs. 0.92%, p=0.566). Conclusions Individuals with heart failure who suffer from an acute ischemic stroke show worse clinical outcomes in terms of mortality, event severity, and duration of hospital stay.
Barrios-Villa Edwin,Pacheco-Flores Brenda,Lozano-Zaraín Patricia,Del Campo-Ortega Rodolfo,de Jesús Ascencio-Montiel Ivan,González-León Margot,Camorlinga-Ponce Margarita,Gaytán Cervantes Francisco Javi 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background The dry root or stem bark of Fraxinus chinensis is a famous herb Qin Pi which is known for its anti-inflammatory, analgesic, anti-tumor, liver protective and diuretic pharmacological effects, the fundamental chemical components are coumarin, phenylethanol glycosides and flavonoids. However, it is difficult to clarify the secondary metabolite synthesis pathway and key genes involved in the pathway because of lack genome information of Fraxinus chinensis. Objective To generate a complete transcriptome of Fraxinus chinensis and to clarify the differentially expressed genes (DEGs) in leaves and stem barks. Methods In this study, full-length transcriptome analysis and RNA-Seq were combined to characterize Fraxinus chinensis transcriptome. Results A total of 69,145 transcripts were acquired and regarded as reference transcriptome, 67,441 transcripts (97.47%) were annotated to NCBI non-redundant protein (Nr), SwissProt, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and eukaryotic orthologous groups (KOG) databases. A total of 18,917 isoforms were annotated to KEGG database and classified to 138 biological pathways. In total, 10,822 simple sequence repeat (SSRs) and 11,319 resistance (R) gene were classified to 18 types, and 3947 transcription factors (TFs) were identified in full-length transcriptome analysis. Additionally, 15,095 DEGs were detected by RNA-seq in leaves and barks, including 4696 significantly up-regulated and 10,399 significantly down-regulated genes. And 254 transcripts were annotated into phenylpropane metabolism pathway containing 86 DEGs and ten of these enzyme genes were verified by qRT-PCR. Conclusion It laid the foundation for further exploration of the biosynthetic pathway of phenylpropanoids and related key enzyme genes.
Enrique Jesús Delgado-Suárez,Rocío Ortíz-López,Wondwossen A. Gebreyes,Marc W. Allard,Francisco Barona-Gómez,María Salud Rubio-Lozano 한국미생물학회 2019 The journal of microbiology Vol.57 No.4
Multi-drug resistant (MDR) non-typhoidal Salmonella (NTS) is increasingly common worldwide. While food animals are thought to contribute to the growing antimicrobial resistance (AMR) problem, limited data is documenting this relationship, especially in low and middle-income countries (LMIC). Herein, we aimed to assess the role of non-clinical NTS of bovine origin as reservoirs of AMR genes of human clinical significance. We evaluated the phenotypic and genotypic AMR profiles in a set of 44 bovine-associated NTS. For comparative purposes, we also included genotypic AMR data of additional isolates from Mexico (n = 1,067) that are publicly available. The most frequent AMR phenotypes in our isolates involved tetracycline (40/44), trimethoprim-sulfamethoxazole (26/44), chloramphenicol (19/44), ampicillin (18/44), streptomycin (16/44), and carbenicillin (13/44), while nearly 70% of the strains were MDR. These phenotypes were correlated with a widespread distribution of AMR genes (i.e. tetA, aadA, dfrA12, dfrA17, sul1, sul2, bla-TEM-1, blaCARB-2) against multiple antibiotic classes, with some of them contributed by plasmids and/or class-1 integrons. We observed different AMR genotypes for betalactams and tetracycline resistance, providing evidence of convergent evolution and adaptive AMR. The probability of MDR genotype occurrence was higher in meat-associated isolates than in those from other sources (odds ratio 11.2, 95% confidence interval 4.5–27.9, P < 0.0001). The study shows that beef cattle are a significant source of MDR NTS in Mexico, highlighting the role of animal production on the emergence and spread of MDR Salmonella in LMIC.