Selective miRNA Expression Profile in Chronic Myeloid Leukemia K562 Cell-derived Exosomes

Feng, Dan-Qin,Huang, Bo,Li, Jing,Liu, Jing,Chen, Xi-Min,Xu, Yan-Mei,Chen, Xin,Zhang, Hai-Bin,Hu, Long-Hua,Wang, Xiao-Zhong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of hematopoietic stem cell scarrying the Philadelphia (Ph) chromosome and an oncogenic BCR-ABL1 fusion gene. The tyrosine kinase inhibitor (TKI) of BCR-ABL1 kinase is a treatment of choice for control of CML. Objective: Recent studies have demonstrated that miRNAs within exosomes from cancer cells play crucial roles in initiation and progression. This study was performed to assess miRNAs within exosomes of K562 cells. Methods: miRNA microarray analysis of K562 cells and K562 cell-derived exosomes was conducted with the 6th generation miRCURYTM LNA Array (v.16.0). Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were also carried out. GO terms and signaling pathways were categorized into 66 classes (including homophilic cell adhesion, negative regulation of apoptotic process, cell adhesion) and 26 signaling pathways (such as Wnt). Results: In exosomes, 49 miRNAs were up regulated as compared to K562 cells, and two of them were further confirmed by quantitative real-time PCR. There are differentially expressed miRNAs between K562 cell derived-exosomes and K562 cells. Conclusion: Selectively expressed miRNAs in exosomes may promote the development of CML via effects on interactions (e.g. adhesion) of CML cells with their microenvironment.

Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli

Dan Feng,Minyang Zhang,Shiyi Tian,Jing Wang,Weiyun Zhu 한국미생물학회 2020 The journal of microbiology Vol.58 No.9

Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease. Therefore, the objective of this study was to evaluate whether chitosan- chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CSZn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli. 36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment. On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage. CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05). 16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05). However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05). The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05). In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and upregulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05). In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.

Hierarchical Colored Petri Nets Based Components for Workflow Systems

Feng Zhou,Rui-Feng Bo,Hong-Zhong Huang,Dan Ling,Qiang Miao 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.10

Workflow systems have benefited the enterprise and customer in many aspects. But with the increasing complexity of the system, workflow design becomes a complicated and time-consuming process. In this paper, we model the system based on the Hierarchical Colored Petri Nets (HCPN) to avoid the complexity of the system, and the super nets of the model are abstracted as independent components to increase the flexibility, acceptability and maintainability of the system. Another important characteristic of this model is its convenience for reuse and workflow mining. The development process of the overhead traveling crane was given to demonstrate the proposed method.

Impact of Cu2O doping on high dielectric properties of CuO ceramics

Dan-Dan Wang,Feng-Zi Zhou,Jing-Xiao Cao,Li-Ben Li,Guo-Ling Li 한국물리학회 2017 Current Applied Physics Vol.17 No.5

Single-phase CuO ceramic samples were prepared with the starting nano powders of CuO þ xCu2O (x ¼ 0, 1, 3, 7% in mole ratio) via solid state reaction method, and characterized by X-ray diffraction, Raman scattering and scanning electron microscopy. For all the samples, the temperature dependences of dielectric constants and losses were measured at the frequencies of 102, 103, 104, 105 and 106 Hz, respectively. With increasing doping content of Cu2O, a strong correlation is demonstrated at given temperature and frequency between the measured dielectric constants and the unit-cell volumes of CuO. The strong correlation is argued in terms of the change in densities of Cu]O defects (e.g. Cu/O vacancies and/or interstitial Cu impurities) due to Cu2O doping, which is supported by the formation energies of Cu]O defects and the corresponding unit-cell volume from first-principles calculations. The high dielectric constant (~103e105) of CuO ceramic is therefore attributed to the reduction in resistance due to Cu/O defects in the grain by Maxwell-Wagner mechanism.

Simulation of Spinal Codes

Danning Feng,Huijun Yue,Xinyue Li,Siyang Tan,Rui Tao,Junbin Lai 한국산학기술학회 2013 SmartCR Vol.3 No.6

Spinal codes are a new kind of adaptive rateless codes. By analyzing the theory, consisting of hash-function encoding, modulation, maximum-likelihood decoding, and puncturing, the data structure was redesigned, and simulation of the whole transmission process was accomplished. The transfer rate was tested with both additive white Gaussian noise channel (AWGN channel) and binary symmetric channel (BSC). The experiment with AWGN demonstrated the rightness of our design and showed that spinal codes are extremely close to the Shannon Limit. What?s more, validity of the data structure was also proven with BSC. These results show that spinal codes are efficient and practical, which will be very useful in the near future.

Spaces of Fragmentation? - Linkages between Gated Community and its Neighborhood

FENG Dan,ZHU Hong,WERNER Breitung 대한지리학회 2008 대한지리학회 컨퍼런스 자료집 Vol.2 No.-

Fulvestrant 250mg versus Anastrozole 1 mg in the Treatment of Advanced Breast Cancer: a Meta-Analysis of Randomized Controlled Trials

Gong, Dan-Dan,Man, Chang-Feng,Xu, Juan,Fan, Yu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Objective: Most patients with advanced breast cancer experience resistance to endocrine treatment and eventual disease progression. This meta-analysis was designed to compare the efficacy and tolerability of fulvestrant 250mg with anastrozole 1mg in postmenopausal women with advanced breast cancer. Methods: Electronic literature databases (Cochrane Library, Medline, and Embase) were searched for randomized controlled trials (RCTs) published prior to August 2013. Only RCTs that compared fulvestrant 250mg to anastrozole 1mg in postmenopausal women with advanced breast cancer were selected. The main outcomes were time to treatment failure (TTF), time to progression (TTP), duration of response (DOR), clinical benefit rate, and tolerability. Results: Four RCTs covering 1,226 patients (fulvestrant, n=621; anastrozole, n=605) were included in the meta-analysis. Fulvestrant increased the DOR compared to anastrozole (HR =1.31, 95% confidence interval [CI] 1.13-1.51). There was no statistically significant difference between fulvestrant and anastrozole in terms of TTF (HR=1.02, 95%CI 0.89-1.17), complete response (RR=1.79, 95%CI, 0.93-3.43), and partial response (RR=0.91, 95%CI 0.69-1.21). As for safety, there was no statistical significance between the two groups for common adverse events. Conclusion: Fulvestrant 250mg is as effective and well-tolerated as anastrozole 1mg treatment for advanced breast cancer in postmenopausal women whose disease progressed after prior endocrine treatment. Thus, fulvestrant may serve as a reasonable alternative to anastrozole when resistance is experienced in breast cancer cases.

Hierarchical Colored Petri Nets Based Components for Workflow Systems

Zhou Feng,Bo Rui-Feng,Huang Hong-Zhong,Ling Dan,Miao Qiang The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.10

Workflow systems have benefited the enterprise and customer in many aspects. But with the increasing complexity of the system, workflow design becomes a complicated and time-consuming process. In this paper, we model the system based on the Hierarchical Colored Petri Nets (HCPN) to avoid the complexity of the system, and the super nets of the model are abstracted as independent components to increase the flexibility, acceptability and maintainability of the system. Another important characteristic of this model is its convenience for reuse and workflow mining. The development process of the overhead traveling crane was given to demonstrate the proposed method.

Improvement of Poly(γ-glutamic acid) Biosynthesis and Quantitative Metabolic Flux Analysis of a Two-stage Strategy for Agitation Speed Control in the Culture of Bacillus subtilis NX-2

Dan Zhang,Zongqi Xu,Hong Xu,Xiaohai Feng,Sha Li,Heng Cai,Yan Wei,Pingkai Ouyang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6

In this study, the production of poly(γ-glutamic acid) by Bacillus subtilis NX-2 (PGA) at different agitation speeds was investigated. Based on the analysis of specific cell growth rate (μ) and specific PGA formation rate (q_p),a two-stage strategy for agitation speed control was proposed. During the first 24 h, an agitation speed of 600rpm was used to maintain a high μ for better cell growth,which then reduced to 400 rpm after 24 h to maintain a high qp to enhance PGA production. Using this method, the maximum concentration of PGA reached 40.5 ± 0.91 g/L and the PGA productivity was 0.56 ± 0.012 g/L/h, which was 17.7 and 9.8% higher, respectively, than the best results obtained when a constant agitation speed was used. The flux distributions and the related enzymes of 2-oxoglutarate could be affected by this two-stage strategy for agitation speed. The activity of isocitrate dehydrogenase and glutamate dehydrogenase at the key node of 2-oxoglutarate increased, and more flux distribution was directed to glutamate. The flux distribution from extracellular to intracellular glutamate also increased and improved PGA production as the glutamate uptake rates increased using the agitation-shift control method.

Improved Carrier Frequency Offset Estimation Algorithms for PUCCH Format 1 in LTE-Advanced

Dan Wang,Youbo Feng,Yiming Lin 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.9

Physical Uplink Control Channel (PUCCH) is an important narrow-band channel in LTE-Advanced system, which carries the uplink control information, such as acknowledge/non-acknowledge (ACK/NACK) bits, downlink channel quality indicator (CQI), HARQ-ACK feedback information, and it requires much higher transmission quality than the other channels. With the aid of orthogonal CDMA, multiple users share the same time-frequency resource, the multiuser interference has been the main factor that limits the transmission quality of the control information [1]. In the high-speed mobile scenario, there exists a large Doppler frequency shift, which leads to loss of code orthogonality and also introduces inter-carrier interference (ICI) within a single SC-FDMA symbol. Besides, the multiuser interference in high-speed mobile environment is severe. In this paper, the carrier frequency offset estimation algorithms are analyzed, and a double-iteration carrier frequency offset (CFO) estimation algorithm is proved to perform the best for PUCCH Format 1 in high-speed mobile scenario. The estimation process is achieved by coarse estimation and fine estimation, which enlarges the estimation range and improves the estimation accuracy.