http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ponce-Garcia, Gustavo,Villanueva-Segura, Olga K.,Garza-Elizondo, Karina,Villegas-Ramirez, Heriberto M.,Fernandez-Salas, Ildefonso,Rodriguez-Sanchez, Iram P.,Dzul-Manzanilla, Felipe,Flores-Suarez, Adri The Korea Society for Parasitology and Tropical Me 2022 The Korean Journal of Parasitology Vol.60 No.3
The head louse Pediculus humanus capitis (De Geer) is a hematophagous ectoparasite that inhabits the human scalp. The infestations are asymptomatic; however, skin irritation from scratching occasionally may cause secondary bacterial infections. The present study determined the presence and frequency of the knockdown resistance (kdr) mutation T929I in 245 head lice collected from Mexico, Peru, and Canada. Head lice were collected manually using a comb in the private head lice control clinic. Allele mutation at T9291 was present in 100% of the total sampled populations (245 lice) examined. In addition, 4.89% of the lice were homozygous susceptible, whereas 6.93% heterozygous and 88.16% homozygous were resistant, respectively. This represents the second report in Mexico and Quebec and fist in Lima.
dos Inocentes Meneses Felipe,Lobo Luís Gustavo Gomes,de Souza Silvia Graciele Hülse,dos Santos Tiago Benedito 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.4
Superoxide dismutase (SOD) is extremely important antioxidant enzyme and plays essential roles in plant growth, development, and response to diferent abiotic stresses. The SODs genes family has been characterized in several plant species; however, there is no report on identifying these genes in Cofea spp. In this study, the objective was to identify and analyze in silico the SODs genes using the three genomes of Cofea spp. We used various bioinformatics tools to perform physicochemical analyses of proteins, gene structure, chromosome mapping, conserved domain, cis-acting elements, syntenic and phylogenetic analyses, and miRNA targets. A total of 32 SODs genes were identifed in the genomes of Cofea spp. (9—C. canephora; 13—C. arabica L.; 9—C. eugenioides), and phylogenetically classifed into 3 subgroups, copper zinc (Cu/ZnSOD/CSD), manganese (Mn-SOD/MSD), and iron (Fe-SOD/FSD), respectively. Physicochemical analyses of the cofee SODs proteins revealed that the length, molecular weight, and pI values varied between species from 105 (CcCSD4) to 317 (CeCSD7) amino acids, 12.00 (CeCSD4) to 34.59 (CeFSD1) kDa, and 5.37 (CeFSD1) at 9.35 pI (CeCSD4). The intron numbers of SODs genes ranged from 1 to 8. Chromosomal mapping revealed an uneven distribution of SODs genes in the chromosomes of the respective cofee species. In addition, we identifed eight stress-responsive cis-elements in the promoters of cofee SODs genes. In addition, we identifed a total of 13, 12, and 11 putative miRNA families, which can target genes encoding SODs in the 3 cofee species. This study will serve as a biotechnological basis for the characterization of SODs genes involved in regulating gene expression under adverse conditions. This allows the development of biotechnological strategies to ensure greater sustainability of cofee production by establishing ways to reduce damage caused by stressful conditions in cofee production.
Three-Dimensional Cell Cultures as a Research Platform in Lung Diseases and COVID-19
da Silva da Costa Felipe Allan,Soares Murilo Racy,Malagutti-Ferreira Maria José,da Silva Gustavo Ratti,Lívero Francislaine Aparecida dos Reis,Ribeiro-Paes João Tadeu 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5
Background: Chronic respiratory diseases (CRD) are a major public health problem worldwide. In the current epidemiological context, CRD have received much interest when considering their correlation with greater susceptibility to SARS-Cov-2 and severe disease (COVID-19). Increasingly more studies have investigated pathophysiological interactions between CRD and COVID-19. Area covered: Animal experimentation has decisively contributed to advancing our knowledge of CRD. Considering the increase in ethical restrictions in animal experimentation, researchers must focus on new experimental alternatives. Two-dimensional (2D) cell cultures have complemented animal models and significantly contributed to advancing research in the life sciences. However, 2D cell cultures have several limitations in studies of cellular interactions. Three-dimensional (3D) cell cultures represent a new and robust platform for studying complex biological processes and are a promising alternative in regenerative and translational medicine. Expert opinion: Three-dimensional cell cultures are obtained by combining several types of cells in integrated and self-organized systems in a 3D structure. These 3D cell culture systems represent an efficient methodological approach in studies of pathophysiology and lung therapy. More recently, complex 3D culture systems, such as lung-on-a-chip, seek to mimic the physiology of a lung in vivo through a microsystem that simulates alveolar-capillary interactions and exposure to air. The present review introduces and discusses 3D lung cultures as robust platforms for studies of the pathophysiology of CRD and COVID-19 and the mechanisms that underlie interactions between CRD and COVID-19. Background: Chronic respiratory diseases (CRD) are a major public health problem worldwide. In the current epidemiological context, CRD have received much interest when considering their correlation with greater susceptibility to SARS-Cov-2 and severe disease (COVID-19). Increasingly more studies have investigated pathophysiological interactions between CRD and COVID-19. Area covered: Animal experimentation has decisively contributed to advancing our knowledge of CRD. Considering the increase in ethical restrictions in animal experimentation, researchers must focus on new experimental alternatives. Two-dimensional (2D) cell cultures have complemented animal models and significantly contributed to advancing research in the life sciences. However, 2D cell cultures have several limitations in studies of cellular interactions. Three-dimensional (3D) cell cultures represent a new and robust platform for studying complex biological processes and are a promising alternative in regenerative and translational medicine. Expert opinion: Three-dimensional cell cultures are obtained by combining several types of cells in integrated and self-organized systems in a 3D structure. These 3D cell culture systems represent an efficient methodological approach in studies of pathophysiology and lung therapy. More recently, complex 3D culture systems, such as lung-on-a-chip, seek to mimic the physiology of a lung in vivo through a microsystem that simulates alveolar-capillary interactions and exposure to air. The present review introduces and discusses 3D lung cultures as robust platforms for studies of the pathophysiology of CRD and COVID-19 and the mechanisms that underlie interactions between CRD and COVID-19.
Vitor José Fonseca,Roberta Rayra Martins Chaves,Felipe Eduardo Baires Campos,Luiz Felipe Lehman,Gustavo Meyer Moraes,Wagner Henriques Castro 대한영상치의학회 2018 Imaging Science in Dentistry Vol.48 No.4
This report presents a clinical case of trauma due to assault with a knife, and describes the importance of using the correct imaging modality in cases of facial penetrating trauma involving the superficial and deep anatomical planes. Penetrating wounds in the maxillofacial region are rare and poorly reported, but can result in serious complications that are difficult to resolve and may compromise the patient’s quality of life, especially when large blood vessels or other vital structures are involved. Thus, it is essential to determine the extent of the affected blood vessels and the proximity of the retained object to the anatomical structures. In this case, digital subtraction angiography was the imaging modality chosen. The use of appropriate imaging examinations allows a proper map of the surgical field, reducing the chances of vascular damage during the surgical procedure.
Silva, Emmanuel Joao Nogueira Leal,Muniz, Brenda Leite,Pires, Frederico,Belladonna, Felipe Goncalves,Neves, Aline Almeida,Souza, Erick Miranda,De-Deus, Gustavo The Korean Academy of Conservative Dentistry 2016 Restorative Dentistry & Endodontics Vol.41 No.1
Objectives: The purpose of this study was to assess the ability of ProTaper Gold (PTG, Dentsply Maillefer) in maintaining the original profile of root canal anatomy. For that, ProTaper Universal (PTU, Dentsply Maillefer) was used as reference techniques for comparison. Materials and Methods: Twenty simulated curved canals manufactured in clear resin blocks were randomly assigned to 2 groups (n = 10) according to the system used for canal instrumentation: PTU and PTG groups, upto F2 files (25/0.08). Color stereomicroscopic images from each block were taken exactly at the same position before and after instrumentation. All image processing and data analysis were performed with an open source program (FIJI). Evaluation of canal transportation was obtained for two independent canal regions: straight and curved levels. Student's t test was used with a cut-off for significance set at ${\alpha}=5%$. Results: Instrumentation systems significantly influenced canal transportation (p < 0.0001). A significant interaction between instrumentation system and root canal level (p < 0.0001) was found. PTU and PTG systems produced similar canal transportation at the straight part, while PTG system resulted in lower canal transportation than PTU system at the curved part. Canal transportation was higher at the curved canal portion (p < 0.0001). Conclusions: PTG system produced overall less canal transportation in the curved portion when compared to PTU system.
Emmanuel Joao Nogueira Leal Silva,Brenda Leite Muniz,Frederico Pires,Felipe Goncalves Belladonna,Aline Almeida Neves,Erick Miranda Souza,Gustavo De-Deus 대한치과보존학회 2016 Restorative Dentistry & Endodontics Vol.41 No.1
Objectives: The purpose of this study was to assess the ability of ProTaper Gold (PTG, Dentsply Maillefer) in maintaining the original profile of root canal anatomy. For that, ProTaper Universal (PTU, Dentsply Maillefer) was used as reference techniques for comparison. Materials and Methods: Twenty simulated curved canals manufactured in clear resin blocks were randomly assigned to 2 groups (n = 10) according to the system used for canal instrumentation: PTU and PTG groups, upto F2 files (25/0.08). Color stereomicroscopic images from each block were taken exactly at the same position before and after instrumentation. All image processing and data analysis were performed with an open source program (FIJI). Evaluation of canal transportation was obtained for two independent canal regions: straight and curved levels. Student's t test was used with a cut-off for significance set at α = 5%. Results: Instrumentation systems significantly influenced canal transportation (p < 0.0001). A significant interaction between instrumentation system and root canal level (p < 0.0001) was found. PTU and PTG systems produced similar canal transportation at the straight part, while PTG system resulted in lower canal transportation than PTU system at the curved part. Canal transportation was higher at the curved canal portion (p < 0.0001). Conclusions: PTG system produced overall less canal transportation in the curved portion when compared to PTU system.
Fonseca, Vitor Jose,Chaves, Roberta Rayra Martins,Campos, Felipe Eduardo Baires,Lehman, Luiz Felipe,Moraes, Gustavo Meyer,Castro, Wagner Henriques Korean Academy of Oral and Maxillofacial Radiology 2018 Imaging Science in Dentistry Vol.48 No.4
This report presents a clinical case of trauma due to assault with a knife, and describes the importance of using the correct imaging modality in cases of facial penetrating trauma involving the superficial and deep anatomical planes. Penetrating wounds in the maxillofacial region are rare and poorly reported, but can result in serious complications that are difficult to resolve and may compromise the patient's quality of life, especially when large blood vessels or other vital structures are involved. Thus, it is essential to determine the extent of the affected blood vessels and the proximity of the retained object to the anatomical structures. In this case, digital subtraction angiography was the imaging modality chosen. The use of appropriate imaging examinations allows a proper map of the surgical field, reducing the chances of vascular damage during the surgical procedure.
Fabiula Patricia Novakoski,Leandro Paiola Albrecht,Alfredo Junior Paiola Albrecht,André Felipe Moreira Silva,Mateus Dalpubel Mattiuzzi,Tamara Thaís Mundt,Eduardo Seity Furlan Kashivaqui,Felipe Gustavo 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.3
Plant lodging is among the factors that limit the productive potential of the soybean crop. Growth regulators or even herbicides may be used in pre- and post-emergence of soybean to minimize the chance of lodging. These products can act to reduce plant height or the number of branches. This study aimed to evaluate the growth and agronomic performance of soybean under the post-emergence application of growth regulators and herbicides. Two experiments were conducted in Palotina, Paraná State (PR), Brazil. A randomized complete block design with four replications was used. Treatments consisted of the application mepiquat chloride, trinexapac, cloransulam, lactofen, and control (without application). Crop injury, chlorophyll indices, and variables related to growth and agronomic performance of soybean crop were evaluated. Post-emergence application of lactofen (36 g a.i. ha −1 ) or cloransulam (33.6 g a.i. ha −1 ) on soybean (V3–V4) reduced the height of soybean plants, but showed no negative eff ect on soybean yield. Therefore, these herbicides are alternatives to reduce the lodging of soybean plants. Post-emergence application of mepiquat chloride (100 g a.i. ha −1 ) was selective to soybean plants, but did not reduce plant growth. Trinexapac application (125 g a.i. ha −1 ) did not reduce plant growth and had a deleterious potential on soybean yield. Therefore, post-emergence application of trinexapac on soybean is not recommended.