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- 검색키워드
- 저자 : de Souza Silvia Graciele Hülse (검색결과 4 건)
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Molybdenum (Mo) transporter genes in Panicoideae species: a genome-wide evolution study
Bavaresco Lorrayne Guimarães,Silva Silviany Angelica Fernandes,de Souza Silvia Graciele Hülse,Ribas Alessandra Ferreira,Tiago Benedito dos Santos 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.3
Molybdenum (Mo) is one important mineral element for plants because it participates actively in many metabolic essential functions like the synthesis of molybdenum cofactor and nitrogen metabolism. Mo is available on soil in an anionic form, and its activity increases with pH elevation. Few studies in the literature reported the identifcation and characterization of Mo transporter genes. Given the above, we describe a comprehensive in silico analysis of Mo transporters in six Panicoideae species. We identifed 15 candidates genes associated with Mo transporters. The subcellular location shows that all predicted genes were present in the plasma membrane. The genomic structure revealed that most of the Mo transporters genes showed no introns, while two sequences of P. virgatum presented one intron. Five conserved motifs and nine putative transmembrane domains were identifed. Phylogenetic analysis revealed two groups (A1 and A2), showing close or conserved phylogenetic relationships. Synteny analysis identifed 45 pairs of syntenic Mo orthologous among the six genomes of Panicoideae species, and purifying selection played a critical role in the evolution of Mo transporter genes. Eforts need to be undertaken to understand and improve molybdenum uptake and utilization in Panicoideae species for the sustainability of these species. This study will serve as a biotechnological basis for the characterization of candidate genes (Mo) involved in the regulation of gene expression under adverse conditions, allowing the development of future strategies to ensure greater sustainability of the important species of Panicoideae.
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Genome-wide in silico analysis of SOD genes in common bean ( Phaseolus vulgaris L.)
Silviany Angelica Fernandes Silva,Fláive Loyze Baldassarini Silva,Alessandra Ferreira Ribas,Silvia Graciele Hülse de Souza,Tiago Benedito dos Santos 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.3
Superoxide dismutases (SODs) are a group of enzymes that play essential roles in catalyzing the dismutation of superoxide radicals to protect cells from oxidative damage caused by various adverse conditions. SODs are classifi ed into three types based on their metal cofactors: Cu/ZnSOD, FeSOD, and MnSOD. This study presents the fi rst genome-wide identifi cation of SOD genes in Phaseolus vulgaris L. Eight SOD genes were identifi ed in the genome of P. vulgaris L., including four Cu / ZnSODs ( CSD ), three FeSODs ( FSD ) and one MnSOD ( MSD ). The protein sequence ranged between 166 and 312 amino acids with 5–8 introns each. Most PvSOD genes are in chromosome 7. The syntenic analysis revealed that PvFSD1 and PvFSD2 genes were derived from segmental duplication. As expected, phylogenetic analyses grouped the PvSOD proteins into three classes based on their metal cofactors: copper/zinc (Cu/Zn)SOD, manganese (Mn)SOD, and iron (Fe)SOD. Expression pattern analysis using RNAseq data indicated that three of the PvSOD genes ( PvMSD1 , PvCSD3 , and PvFSD1 ) were detected in majority of tissues and developmental stages. This study provides a comprehensive analysis about SOD genes in P. vulgaris L. and new resources for future studies on the functional analysis of PvSOD genes in this species of great agronomic importance.
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Bonacina Cristine,Nascimento Andressa Bezerra,Barbosa Letícia Neris,de Souza Silvia Graciele Hülse,Magalhães Hélida Mara 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2
During cultivation, environmental factors can cause changes in the metabolism of Curcuma longa L. that limit its development. This study evaluated the efects of diferent sodium chloride (NaCl) concentrations on the leaf anatomy, stomatal features, and biochemical activity of C. longa L. plants. The experiment was conducted in a greenhouse, using rhizomes of C. longa L. planted in plastic pots containing soil, sand, and organic matter (2:1:1). After 180 days of planting, salt stress using NaCl (50, 100, and 150 mM) was induced and a control treatment was made with water. At 150 days post treatment, the morphoanatomical variables of the leaves were evaluated, and biochemical analyses of the leaves and rhizomes were performed. The leaves exposed to intermediate salt concentrations (50 and 100 mM) had fewer hypodermic cells and larger spaces between the cells in the spongy parenchyma. In the 150 mM NaCl group, there was an increase in the total leaf thickness caused by an increase in hypodermic cells. The percentage of closed stomata increased in the 50 mM (85%) and 100 mM (80%) NaCl treatments, and the polar diameter of the cells (1.22%) was reduced in the presence of 150 mM NaCl. Biochemical analyses revealed diferent responses for rhizomes and leaves. The total sugars in the rhizomes increased in the presence of ≥50 mM NaCl, probably because of the need to generate more energy for use in secondary metabolite synthesis pathways. The antioxidative activity and phenolic compounds also increased at the highest NaCl concentrations. In conclusion, we have classifed C. longa L. as a plant that is tolerant to saline stress, owing to the metabolic balance and water-loss barrier provided by the rhizome to mitigate stress damage. Our results showed that C. longa L. plants were tolerant to the high salt concentrations tested in this study (100 and 150 mM).
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dos Inocentes Meneses Felipe,Lobo Luís Gustavo Gomes,de Souza Silvia Graciele Hülse,dos Santos Tiago Benedito 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.4
Superoxide dismutase (SOD) is extremely important antioxidant enzyme and plays essential roles in plant growth, development, and response to diferent abiotic stresses. The SODs genes family has been characterized in several plant species; however, there is no report on identifying these genes in Cofea spp. In this study, the objective was to identify and analyze in silico the SODs genes using the three genomes of Cofea spp. We used various bioinformatics tools to perform physicochemical analyses of proteins, gene structure, chromosome mapping, conserved domain, cis-acting elements, syntenic and phylogenetic analyses, and miRNA targets. A total of 32 SODs genes were identifed in the genomes of Cofea spp. (9—C. canephora; 13—C. arabica L.; 9—C. eugenioides), and phylogenetically classifed into 3 subgroups, copper zinc (Cu/ZnSOD/CSD), manganese (Mn-SOD/MSD), and iron (Fe-SOD/FSD), respectively. Physicochemical analyses of the cofee SODs proteins revealed that the length, molecular weight, and pI values varied between species from 105 (CcCSD4) to 317 (CeCSD7) amino acids, 12.00 (CeCSD4) to 34.59 (CeFSD1) kDa, and 5.37 (CeFSD1) at 9.35 pI (CeCSD4). The intron numbers of SODs genes ranged from 1 to 8. Chromosomal mapping revealed an uneven distribution of SODs genes in the chromosomes of the respective cofee species. In addition, we identifed eight stress-responsive cis-elements in the promoters of cofee SODs genes. In addition, we identifed a total of 13, 12, and 11 putative miRNA families, which can target genes encoding SODs in the 3 cofee species. This study will serve as a biotechnological basis for the characterization of SODs genes involved in regulating gene expression under adverse conditions. This allows the development of biotechnological strategies to ensure greater sustainability of cofee production by establishing ways to reduce damage caused by stressful conditions in cofee production.
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