Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis

Dachuan Fan,Xiangdong Wang,Min Wang,Yang Wang,Liang Zhang,Ying Li,Erzhong Fan,Feifei Cao,Koen Van Crombruggen,Luo Zhang 대한천식알레르기학회 2016 Allergy, Asthma & Immunology Research Vol.8 No.3

Purpose: Group 2 innate lymphoid cells (ILC2s) are a novel population of lineage-negative cells that induce innate type 2 responses by producing the critical Th2-type cytokines IL-5 and IL-13 in response to IL-25 and IL-33 stimulation. ILC2s accumulation in the peripheral blood of patients with allergic rhinitis (AR) is controversial; the precise role of ILC2s in the immunopathogenesis of AR is still not clear. We investigated the role of ILC2s in phenotypic AR sensitized to distinct allergens. Methods: Flow cytometric analysis of the peripheral blood of 7 healthy controls (HCs), 9 patients monosensitized to house dust mite (HDM), and 8 patients monosensitized to mugwort was performed to quantify ILC2s frequency. Peripheral blood mononuclear cells (PBMCs) were isolated from HDM-AR and mugwort-AR patients, and Lineage- and Lineage+ cells were separated using a fluorescence-activated cell sorter (FACS). IL-5 and IL-13 levels in the supernatants of PBMCs, and Lineage- and Lineage+ cells stimulated with IL-25 and/or IL-33 combined with IL-2 in vitro were assessed using the Milliplex magnetic bead kit. Results: The percentage of ILC2s was significantly elevated in HDM-AR patients compared to mugwort-AR patients and HCs, while no significant difference was found between mugwort-AR patients and HCs. IL-33±IL-25 plus IL-2 induced a significantly greater release of IL-5 and IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. IL-25 plus IL-2 also induced a significantly greater release of IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. Stimulation with IL-33 and/or IL-25 combined with IL-2 also induced a significantly greater IL-5 and IL-13 release from Lineage- cells compared to Lineage+ cells. Conclusions: AR patients sensitized to HDM or mugwort allergen have distinct phenotypic and functional profiles in ILC2s frequencies. ILC2s mediate major type 2 immunity in the development of HDM-AR and may be a potential therapeutic target.

DETERMINATION OF AMINOGLYCOSIDE ANTIBIOTICS BY A COLORIMETRIC METHOD BASED ON THE AGGREGATION OF GOLD NANOPARTICLES

RUIYONG WANG,SHUMIN FAN,RUIQIANG WANG,RUI WANG,HUANJING DOU,LVJING WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.4

A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodified gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67–33.93 ng mL-1, 13.33–66.67 ng mL-1 and 20–180 ng mL-1, respectively. The corresponding limit of detection (3σ) was 0.354 ng mL-1, 0.999 ng mL-1 and 0.579 ng mL-1, respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.

User Commitment to Blockchain-Based Social Media Platforms from the Perspective of Perceived Justice Regarding the Token Reward System: the Mediating Role of Psychological Ownership

Xue FAN(Xue FAN),Seongtaek RIM(Seongtaek RIM),Mengmeng WANG(Mengmeng WANG) 동아시아경상학회 2023 The East Asian Journal of Business Economics Vol.11 No.1

Purpose – In this study, we aimed to theorize blockchain-based social media platform users’ commitment by examining the impact of their perceived justice of the token reward system. In addition, this study applied psychological ownership theory to verify the underlying mechanism between users’ perceptions of justice and their commitment to the platforms. Research design, data, and methodology – To empirically test our conceptual framework in the study, we collected data through a web-based survey approach from the responses of 385 users who had experience with blockchainbased social media platforms. We employed a structural equation modeling approach to empirically test our proposed hypotheses. Result – The results indicated that distributive justice and informational justice have positive effects on user commitment. The results also showed that psychological ownership plays an important role in mediating the relationship between users’ sense of distributive justice and commitment, and between procedural justice and commitment. The findings provided a better understanding of the sense of justice and user commitment in a blockchain-based social media environment. Conclusion – This study represents a preliminary attempt to theorize and empirically examine blockchain-based social media platform users’ commitment. This study provided important contributions to the literature on how the effect of users’ sense of justice in a reward system affects their commitment to blockchain-based social media platforms.

A Joint Control Strategy for Automobile Active Grille Shutter and Cooling Fan

Liu Chuanbo,Wang Zhengju,Fan Chaojie,Zhang Ruonan,Man Xingjia 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.6

As an emerging technology improving fuel consumption, active grille shutter (AGS) has been applied in engine cooling system. For the reason of unreasonable matching method of grille opening and cooling fan, the problem of engine overcooling is widespread. As components with similar function of adjusting cooling air flow, the AGS and the cooling fan are separated from each other in parameter matching and operation which reduce cooling efficiency and fuel economy. To achieve precise cooling and improve fuel economy, a joint control strategy for AGS and cooling fan was proposed by using computational fluid dynamics. In order to verify the feasibility of the strategy, the fuel economy tests were carried out at 0 km/h, 90 km/h and 120 km/h, respectively. The results showed that the fuel consumption under the three working conditions was reduced by 0.01 L/ 100 km, 0.07 L/ 100 km and 0.13 L/ 100 km, respectively, indicating that the proposed strategy can significantly reduce fuel consumption.

전후 일본의 국가 대전략과 북일관계

강용범 ( Jiang Long-fan ) , 왕해범 ( Wang Hai-fan ) 조선대학교 동북아문제연구소 2016 동북아연구 Vol.31 No.1

역사적으로 한반도는 일본이 무력으로 지역패권을 도모하고 이른바 “동아시아공영권”의 야망을 실현하는 전초기지가 되어왔다. 제2차세계대전후 일본은 보통국가의 지위와 신분을 회복하고 나아가 아시아지역 강대국으로 부상하는 것을 국가전략의 최종목표로 삼아왔다. 이러한 국가전략을 실현하는 과정에 과거 식민통치에 대한 청산은 비켜설 수 없는 걸림돌이 되었다. 냉전이 한창이던 1965년, 미국의 적극적인 중재하에 한·일양국은 국교정상화를 실현하였다. 따라서 북한과의 관계개선은 전후 일본외교의 중요한 과제로 부상하게 되었다. 일본의 정치가들은 흔히 일본은 전략이 없는 국가라고 한다. 허나 냉전 이후 20여년간 전개되어 온 일본의 대북정책을 살펴보면 일본의 국가전략이 얼마나 교묘한가를 알 수 있다. 우선 북한과의 국교정상화를 통해 정상국가로서의 신분과 지위를 회복하고, 다음으로 북한의 “안전위협”을 빌미로 “군사대국화”를 실현하며, 나아가 6자회담에 끼어들어 한반도다자안보협의에 깊숙이 개입함으로써 정치, 군사 대국화를 위한 기틀을 마련하였다. 뿐만 아니라 일본의 대북정책은 반성과 사죄 그리고 배상이라는 근본적인 문제를 떠나 북한의 핵·미사일과 납치문제에만 집착하고 있다. 더욱이 일본은 중국이나 한국과의 관계가 악화되어 외교적으로 고립될 경우에는 북한과의 관계 완화를 통해 위기를 탈출하려 시도하고, 안보법안과 헌법개정을 위해선 “북한위협론”을 들먹여 제재를 강화하는 등 교묘한 전략을 구사하여왔다. 제4차 북핵실험 후 북·일관계는 또다시 최악의 위기를 맞고 있다. 북한은 일본인납치피해자에 대한특별조사위원회를 해체하였고 일본은 유엔안보리제재는 물론 독자적이고 더욱 강경한 제재를 실시하고 있다. 헌법개정을 본격적으로 추진하고 있는 아베 내각으로 말하면 북한위협이 고조되고 양국관계가 악화되는 것이 더욱 바람직한 전략적 선택일 수도 있다. In history, the Korean peninsula has been an advanced base of Japan to pursue local hegemony through force and realize an ambition to achieve Mutual Prosperity in East Asia. After World War II, Japan has had a final goal of recovering the position and status of a normal country and furthermore, raising its status to one of the top countries in Asia. In the process of realizing such a national strategy, its colonial rule in the past has become a setback which can not be overcome. In 1965 when the Cold War was at its peak period, under the active intervention of the U.S.A, South Korea and Japan normalized diplomatic relations. Therefore, a better relationship with North Korea has become an important task for Japan`s diplomacy before and after the war. Japan`s politicians said that Japan does not have any strategies. However, to look at the policies against North Korea which have been developed for about 20 years after the Cold War, it can be understood how clever Japanese strategies against other countries are. First of all, through normalization of diplomatic relations with North Korea, it recovered the status and position of a normal country. Then, under the pretext of the security threat of North Korea, it realized “Military Power” and furthermore it joined in six-party talks to intervene in the multilateral security talks and were prepared for a great military power. As well, Japan`s policy against North Korea has adhered to nuclear technology ,missiles, and kidnapping out of the fundamental problems of reflection, apology and compensation. In addition, as Japan has had worse relations with China and South Korea, and is isolated diplomatically, it attempted to escape from this crisis through mitigation of relations with North Korea, and for security law and constitution revision, it mentioned the North Korean Threat Issue to reinforce restraint. After the 4th North Korea Nuclear Test, relations between North Korea and Japan faced the worst crisis. North Korea dismantled a special investigation committee into Japanese kidnappings and Japan has implemented more independent and resolute restraints as well as restraints in the U.N. Security Council. To the Abe administration which has actively executed Constitutional reform, it is suggested that the severe threat of North Korea and aggravated relations between the two countries would be a more desirable strategy.

AN ULTRA-LUMINOUS QUASAR AT <i>z</i> = 5.363 WITH A TEN BILLION SOLAR MASS BLACK HOLE AND A METAL-RICH DLA AT <i>z</i> ∼ 5

Wang, Feige,Wu, Xue-Bing,Fan, Xiaohui,Yang, Jinyi,Cai, Zheng,Yi, Weimin,Zuo, Wenwen,Wang, Ran,McGreer, Ian D.,Ho, Luis C.,Kim, Minjin,Yang, Qian,Bian, Fuyan,Jiang, Linhua IOP Publishing 2015 ASTROPHYSICAL JOURNAL LETTERS - Vol.807 No.1

<P>We report the discovery of an ultra-luminous quasar J030642.51+185315.8 (hereafter J0306+1853) at redshift 5.363, which hosts a supermassive black hole with M-BH = (1.07 +/- 0.27) x 10(10) M-circle dot. With an absolute magnitude M-1450 = -28.92 and a bolometric luminosity L-bol similar to 3.4 x 10(14) L-circle dot, J0306+1853 is one of the most luminous objects in the early universe. It is not likely to be a beamed source based on its small flux variability, low radio loudness, and normal broad emission lines. In addition, a z = 4.986 damped Ly alpha system (DLA) with [M/H] = -1.3 +/- 0.1, among the most metal-rich DLAs at z greater than or similar to 5, is detected in the absorption spectrum of this quasar. This ultra-luminous quasar puts strong constraints on the bright end of the quasar luminosity function and massive end of the black hole mass function. It will provide a unique laboratory for the study of BH growth and the co-evolution between a BH and the host galaxy with multi-wavelength follow-up observations. The future high-resolution spectra will give more insight into the DLA and other absorption systems along the line of sight of J0306+1853.</P>

A Randomized Controlled Trial Comparing Clinical Outcomes and Toxicity of Lobaplatin- Versus Cisplatin-Based Concurrent Chemotherapy Plus Radiotherapy and High-Dose-Rate Brachytherapy for FIGO Stage II and III Cervical Cancer

Wang, Ji-Quan,Wang, Tao,Shi, Fan,Yang, Yun-Yi,Su, Jin,Chai, Yan-Lan,Liu, Zi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: We designed this randomized controlled trial (RCT) to assess whether lobaplatin-based concurrent chemotherapy might be superior to cisplatin-based concurrent chemotherapy for FIGO stage II and III cervical cancer in terms of efficacy and safety. Materials and Methods: This prospective, open-label RCT aims to enroll 180 patients with FIGO stage II and III cervical cancer, randomly allocated to one of the three treatment groups (cisplatin $15mg/m^2$, cisplatin $20mg/m^2$ and lobaplatin $35mg/m^2$), with 60 patients in each group. All patients will receive external beam irradiation (EBRT) and high-dose-rate intracavitary brachytherapy (HDR-ICBT). Patients in cisplatin $15mg/m^2$ and $20mg/m^2$ groups will be administered four cycles of $15mg/m^2$ or $20mg/m^2$ cisplatin intravenously once weekly from the second week to the fifth week during EBRT, while patients inthe lobaplatin $35mg/m^2$ group will be administered two cycles of $35mg/m^2$ lobaplatin intravenously in the second and fifth week respectively during pelvic EBRT. All participants will be followed up for at least 12 months. Complete remission rate and progression-free survival (PFS) will be the primary endpoints. Overall survival (OS), incidence of adverse events (AEs), and quality of life will be the secondary endpoints. Results: Between March 2013 and March 2014, a total of 61 patients with FIGO stage II and III cervical cancer were randomly assigned to cisplatin $15mg/m^2$ group (n=21), cisplatin $20mg/m^2$ group (n=21) and lobaplatin $35mg/m^2$ group (n=19). We conducted a preliminary analysis of the results. Similar rates of complete remission and grades 3-4 gastrointestinal reactions were observed for the three treatment groups (P=0.801 and 0.793, respectively). Grade 3-4 hematologic toxicity was more frequent in the lobaplatin group than the cisplatin group. Conclusions: This proposed study will be the first RCT to evaluate whether lobaplatin-based chemoraiotherapy will have beneficial effects, compared with cisplatin-based chemoradiotherapy, on complete remission rate, PFS, OS, AEs and quality of life for FIGO stage II and III cervical cancer.

High-fidelity bioelectronic muscular actuator based on porous carboxylate bacterial cellulose membrane

Wang, Fan,Jin, Zhen,Zheng, Shaohui,Li, Hao,Cho, Sunghoon,Kim, Hyeon Joe,Kim, Seong-Jun,Choi, Eunpyo,Park, Jong-Oh,Park, Sukho Elsevier 2017 Sensors and actuators. B Chemical Vol.250 No.-

<P><B>Abstract</B></P> <P>Human-friendly electronic products, such as smart mobile phones, soft haptic devices, wearable electronics, and implantable or disposal biomedical devices, will require the use of high-performance durable soft electroactive actuators with eco-friendly, biocompatible, and biodegradable functionalities. Here, we report a high-fidelity bioelectronic muscular actuator based on porous carboxylate bacterial cellulose (CBC) membranes fabricated using the facile zinc oxide (ZnO) particulate leaching (PL) method. The proposed CZ-PL muscular actuator exhibits large deformation, low actuation voltage, fast response, and high-durability in open air environment. In particular, the CZ-PL membrane shows a dramatic increase in the ionic liquid uptake ratio, ionic exchange capacity, and ionic conductivity of up to 70.63%, 22.50%, and 18.2%, respectively, for CBC, resulting in a 5.8 times larger bending deformation than that of the pure CBC actuator. The developed high-performance CZ-PL muscular actuator can be a promising candidate for meeting the tight requirements of human-friendly electronic devices such as wearable devices, biomimetic robots, and biomedical active devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We developed a novel dry-type muscular actuator based on porous carboxylate bacterial cellulose (CBC) membrane. </LI> <LI> The porous CBC membrane was prepared by ZnO particulate leaching method. </LI> <LI> The proposed actuator showed better actuation performance than that of the pure CBC actuator. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

General method of reducing switching voltage stress without extra voltage‑loop control for related switching‑capacitor high‑gain converters

Fan Wang,Yubin Wang,Menghan Chu,Chuhao Gao 전력전자학회 2024 JOURNAL OF POWER ELECTRONICS Vol.24 No.4

High-gain converters have been used on many occasions as interfaces for multiple energy conversions. Among these converters, a family of switching-capacitor high-gain converters is widely used due to its low cost, small volume, low loss, spontaneous capacitor voltages balance, and spontaneous inductor currents average. However, one or more of the switches in these converters suffer from the issue of withstanding higher voltage stresses than the other switches, which may limit the applications of these converters at high voltages. To solve this issue, a general method for reducing the switching voltage stresses for this high-gain converter family is proposed. This method can improve many kinds of high-gain converters, which can reduce the switching voltage stresses. Based on this method, a two-phase low-stress high-gain converter is proposed. The voltage stresses of all the switches in the proposed converter do not exceed half of the output voltage of the converter. Then, based on the proposed converter feature, a control method is proposed that can make the proposed converter have the automatic capacitor voltage balancing characteristic without an extra voltage-loop control. Finally, the proposed method is verified by prototype experiments.

Fine mapping and candidate gene analysis of the dwarf gene d162(t) in rice (Oryza sativa L.)

Fan-tao Zhang,Xiao-ling Gao,Ping-rong Wang,Chang-hui Sun,Bing Wang,Xiu-lan Li,Jian-qing Zhu,Xiao-jian Deng 한국유전학회 2011 Genes & Genomics Vol.33 No.1

In our previous study, d162(t), a single recessive gene, which caused rice dwarf mutant, had been mapped on the short arm of chromosome 3. In this study, the d162(t) gene was fine mapped to a confined region about 0.82 cM by RM14641 and RM3134, and co-segregated with InDel361-2, InDel361-3,InDel361-5, RM14645, RM1022 and RM14643, where no known gene involved in plant height has been identified. Based on the annotation results of TIGR, dozens of open reading frames (ORFs) were predicted in this region, among them,five ORFs were the most possible genes related to the phenotype. In these ORFs, Os03g13010, related to U-box domain containing protein, had a 62bp segment deletion in the coding region in 162d (mutant type, MT). The results of RT-PCR showed that the transcriptional level of Os03g13010was significantly different between Shuhui162 (wild type, WT)and 162d (MT). Therefore, the gene (Os03g13010) encoding a U-box domain containing protein was considered as the candidate gene of d162(t).