Yacon Flour and Bifidobacterium longum Modulate Bone Health in Rats

Fabiana Carvalho Rodrigues,Adriano Simo˜es Barbosa Castro,Vı´vian Carolina Rodrigues,Se´rgio Antoˆnio Fernandes,Edimar Aparecida Filomeno Fontes,Taˆnia Toledo de Oliveira,He´rcia Stampini Duarte Marti 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.7

Yacon flour has been considered a food with prebiotic potential because of the high levels of fructooligosaccharides, which allows for its use in formulating synbiotic foods. The purpose of this study was to evaluate the effect of yacon flour and probiotic (Bifidobacterium longum) on the modulation of variables related to bone health. Thirty-two Wistar rats were divided into 4 groups: control, yacon flour, diet + B. longum, and yacon flour + B. longum. After euthanasia, the bones were removed for analysis of biomechanical properties (thickness, length, and strength of fracture) and mineral content (Ca, Mg, and P); the cecum was removed for analysis of the microbiota and short-chain fatty acids. Tibia Ca, P, and Mg content was significantly (P < .05) higher in groups fed diet + B. longum, yacon flour + B. longum than in the control group. An increase in fracture strength was observed in the yacon flour (8.1%), diet + B. longum (8.6%), and yacon flour + B. longum (14.6%) in comparison to the control group. Total anaerobe and weight of the cecum were higher (P < .05) in rats consuming the yacon flour diet compared with the other groups. Cecal concentration of propionate was higher in all experimental groups compared with the control (P < .05). Yacon flour in combination with B. longum helped increase the concentration of minerals in bones, an important factor in the prevention of diseases such as osteoporosis.

Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice

Fabiana M. Rinaldi,Emanuelle B. Gaspar,Luciana T. Brito,Elizabeth De Gaspari 대한백신학회 2021 Clinical and Experimental Vaccine Research Vol.10 No.2

Purpose: In the present study, meningococcal serogroup B outer membrane vesicles (OMVs) were associated with bilayer fragments of a cationic lipid, dioctadecyldimethylammonium (DDA-BF), used as adjuvant, in an antigenic preparation tested in adult female outbred mice. This adjuvant was compared to the traditional adjuvant aluminum hydroxide. Materials and Methods: The potential in generating humoral response was evaluated by enzyme-linked immunosorbent assay (ELISA). Individual serum was collected and immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b were quantified. Analyses were carried out 15 and 60 days after immunization. Antibodies avidity index were also analyzed by ELISA. Immunoblot and dot-ELISA were carried out to evaluate specific reaction for homologous strains and cross-reactive antigens present in other meningococcal strains isolated in 2011–2012 year, in Brazil. Delayed type hypersensitivity was used as indicative of cellular immunity and compared between two experimental groups, 24 hours after homologous strain challenge. Results: The OMVs of Neisseria meningitidis, and N. lactamica (related species) were characterized by electrophoretic separation of proteins in 13% polyacrylamide gel. The strains presented antigens in the range of 8 to 130 kDa, showing a heterogeneous protein migration pattern. In the group immunized with OMVs/DDA-BF, we found no significant production of total IgG 15 days after the first immunization. On the other hand, 60 days after first immunization both adjuvants act benefiting total IgG production similarly. The antibodies of the IgG isotype produced by animals immunized after one or two doses after first immunization, showed intermediate and high avidity, independent on the adjuvant used. In both experimental groups the swelling of the footpads was significantly higher than those of the controls, suggesting that only one dose was enough to stimulate the generation of cellular immunity. Conclusion: The use of this cationic adjuvant for N. meningitidis OMVs preparation revealed good potential for future new antigen preparation for N. meningitidis vaccine.

Buenos Aires

Fabiana Gadano 한국디자인학회 2018 한국디자인학회 국제초대전도록 Vol.2018 No.6

Antidepressant-Like Activity of Sonchus oleraceus in Mouse Models of Immobility Tests

Fabiana Cardoso Vilela,Marina de Mesquita Padilha,Geraldo Alves-da-Silva,Roseli Soncini,Alexandre Giusti-Paiva 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1

The aim of the present work is to evaluate the putative antidepressant-like effects of hydroethanolic and dichloromethanic extracts from the aerial parts of Sonchus oleraceus (Family Asteraceae) on the performance of male mice in the forced swimming test (FST) and tail suspension test (TST) models predictive of depression. The hydroethanolic and dichloromethanic extracts, both in doses of 30, 100, and 300mg/kg, were orally administered 1 hour before carrying out the FST or the TST. The immobility time in both the FST and the TST was significantly reduced by acute oral treatment with the extracts (dose range, 100–300mg/kg), without accompanying changes in ambulation, as assessed in an open-field test. This excluded the possibility that the effect of the extracts is due to an activation of locomotion. The efficacy of the extracts was found to be comparable to that of amitriptyline (10mg/kg, p.o.). The present study provides evidence for an antidepressant-like effect of the active principle(s) present in the extracts of S. oleraceus in mice. Therefore, a standardized S. oleraceus extract or its purified constituents could be of potential interest for the treatment of depressive disorders.

Optimization of Production, Biochemical Characterization and In Vitro Evaluation of the Therapeutic Potential of Fibrinolytic Enzymes from a New Bacillus Amyloliquefaciens

Fabiana América Silva Dantas de Souza,Amanda Emmanuelle Sales,Pablo Eugênio Costa e Silva,Raquel Pedrosa Bezerra,Germana Michelle de Medeiros e Silva,Janete Magali de Araújo,Galba Maria de Campos Taka 한국고분자학회 2016 Macromolecular Research Vol.24 No.7

The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to produce fibrinolytic proteases. Thirty bacteria were confirmed as producers of fibrinolytic enzymes, the best results obtained for the strain Bacillus amyloliquefaciens UFPEDA 485. The optimization of the enzyme production conditions was done by a central composite design (CCD) star 23 that allowed to define the optimal conditions for soybean flour and glucose concentrations and agitation rate. The highest fibrinolytic activity (FA) of 813 U mL-1 and a degradation of blood clot in vitro of 62% were obtained in a medium with 2% (w/v) of soybean flour and 1% (w/v) glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37 °C. The obtained fibrinolytic enzyme was characterized biochemically. Fibrinolytic activity was inhibited by PMSF (fluoride methylphenylsulfonyl - C7H7FO2S) 91.52% and EDTA (ethylenediaminetetraacetic acid - C10H16N2O8) 89.4%, confirming to be a serine- metallo protease. The optimum pH and temperature were 7.0 and 37 oC, respectively, and the enzyme was stable for 12 h. The fibrinolytic activity at physiological conditions of this enzyme produced by Bacillus amyloliquefaciens UFPEDA 485, as well as its long term stability, demonstrate that it has suitable characteristics for human and veterinary applications, and promises to be a powerful drug for the treatment of vascular diseases.

Piglets' Surface Temperature Change at Different Weights at Birth

Caldara, Fabiana Ribeiro,dos Santos, Luan Sousa,Machado, Sivanilza Teixeira,Moi, Marta,de Alencar Naas, Irenilza,Foppa, Luciana,Garcia, Rodrigo Garofallo,de Kassia Silva dos Santos, Rita Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.3

The study was carried out in order to verify the effects of piglets' weight at birth on their surface temperature change (ST) after birth, and its relationship with ingestion time of colostrum. Piglets from four different sows were weighed at birth and divided into a totally randomized design with three treatments according to birth weight (PBW): T1 - less than 1.00 kg, T2 - 1.00 to 1.39 kg, and T3 - higher than or equal to 1.40 kg. The time spent for the first colostrum ingestion was recorded (TFS). Images of piglets' surface by thermal imaging camera were recorded at birth (STB) and 15, 30, 45, 60, and 120 min after birth. The air temperature and relative humidity were recorded every 30 min and the indexes of temperature and humidity (THI) were calculated. A ST drop after 15 min from birth was observed, increasing again after sixty minutes. Positive correlations were found between the PBW and the ST at 30 and 45 min after birth. The PBW was negatively correlated with the TFS. The THI showed high negative correlations (-0.824 and -0.815) with STB and after 15 min from birth. The piglet's surface temperature at birth was positively correlated with temperature thereof to 15 min, influencing therefore the temperatures in the interval of 45 to 120 min. The birth weight contributes significantly to postnatal hypothermia and consequently to the time it takes for piglets ingest colostrum, requiring special attention to those of low birth weight.

Carcass Characteristics and Qualitative Attributes of Pork from Immunocastrated Animals

Caldara, Fabiana Ribeiro,Moi, Marta,Santos, Luan Sousa Dos,Paz, Ibiara Correia De Lima Almeida,Garcia, Rodrigo Garofallo,Naas, Irenilza De Alencar,Fernandes, Alexandre Rodrigo Mendes Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.11

An investigation was carried out to assess the carcass characteristics and meat quality aspects of immunocastrated male pigs of medium genetic potential for lean meat deposition in carcass (53 to 55%). When the crossbred Large White x Landrace pigs (n = 45) were 70 days old, they were distributed in a totally randomized design in three treatments (castrated males, females and immunocastrated males) with three replicates of five animals. The pigs were slaughtered when they were 140 days old. Carcass temperature and pH were recorded twice, at 45 min and 24 h after slaughter. The carcasses were evaluated for hot and cold carcass yield, commercial cuts yield, length and depth, back fat thickness, loin eye area and lean meat percentage. The Longissimus dorsi muscle was extracted for analysis of color ($L^*$, $a^*$, $b^*$), exudate loss, cooking loss and centesimal and sensorial analysis of the meat. There were no significant differences for the evaluated parameters between castrated males, immunocastrated males and females, except for backfat thickness between the 7th and 8th thoracic vertebra and the point P2 (lower for immunocastrated males) and carcass temperature at 45 min post slaughter (higher in immunocastrated males), however, this did not interfer with the rate of pH decrease post mortem and the meat quality. The results from this research did not indicate a benefit of immunocastration on carcass characteristics of pigs of medium genetic potential for lean meat deposition in carcass, when compared to surgical castration.

Fabrication and electrical characterization of vertical electrolyte transistor

Keli Fabiana Seidel 한국물리학회 2020 Current Applied Physics Vol.20 No.11

Here, it is reported a vertical electrolyte transistor (VET) whose structure is based on stacked layers: bottom contact → channel → permeable intermediate electrode → ion gel (electrolyte gate dielectric) → gate top contact. This VET depicts versatility to work with two different mode of operation, as: Electrolyte-Gated Vertical Organic Field Effect Transistor (Electrolyte-Gated VOFET) or Vertical Organic Electrochemical Transistor (VOECT). The difference is regarding to the transistor transconductance that occurs due to induced charge carriers or ionic current, respectively. Here, the focus is on giving special attention to the electrical characterization of the VET diode cell in comparison to the VET full architecture. These measurements are able to provide the baseline output current from a VET and consequently distinguish it from possibles different modes of operation created by ionic species. Both modes of operation show that this VET is able to work at very low voltage range and drive a high current density.

Comparison Between Extracorporeal Shock Wave Therapy and Intra-articular Hyaluronic Acid Injections in the Treatment of First Carpometacarpal Joint Osteoarthritis

Francesco Ioppolo,Fabiana Saracino,Rosaria Sabrina Rizzo,Giampaolo Monacelli,Danilo Lanni,Luca Di Sante,Angelo Cacchio,Valter Santilli,Teresa Venditto 대한재활의학회 2018 Annals of Rehabilitation Medicine Vol.42 No.1

Objective To compare extracorporeal shockwave therapy (ESWT) with hyaluronic acid (HA) intra-articular injections in terms of pain relief, improvement in hand function, and strength in subjects with first carpometacarpal (CMC) joint osteoarthritis. Methods Fifty-eight patients received either focused ESWT or HA injection once a week for 3 consecutive weeks. In the ESWT group, 2,400 consecutive pulses were performed during each treatment session using a frequency of 4 Hz and an an energy flux density of 0.09 mJ/mm2. The HA group underwent one cycle of three injections of 0.5 cm3 HA. The main outcome measures were pain and hand function as measured by the visual analogue scale (VAS) and Duruoz Hand Index (DHI), respectively. The secondary outcomes were grip and pinch strength. Each assessment was performed at baseline, at the end of treatment, and at 3- and 6-month follow-up visits. Results According to VAS and DHI scores, a significant change in test performance was observed over time in both groups (p<0.001), with a greater average improvement in painful symptomatology at the 6-month follow-up in the ESWT group. A significant improvement in strength was observed in both groups, but the ESWT group showed better results on the pinch test starting immediately at the end of treatment. Conclusion The use of ESWT in patients with first CMC joint osteoarthritis leads to a reduction in pain, an improvement in pinch test performance that persists for at least 6 months, and a decrease in hand disability up to the 6-month follow-up visit.

Grape juice by-products extracted by ultrasound and microwave-assisted with different solvents: a rich chemical composition

Vania Pezzini,Fabiana Agostini,Franco Smiderle,Luciana Touguinha,Mirian Salvador,Sidnei Moura 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3

By-products of the grape juice industry containvaluable compounds. The current work produced bioactive-enriched extracts from by-products of the grape juice,through three different extraction methods. Yields andchemical compositions varied, according to the extractionmethod (ultrasound, microwave, liquid–liquid). High-efficiencyliquid chromatography with UV–Vis and high-resolutionmass spectrometry characterised were used forchemical characterization, with glycosylated flavonoidsevident. The crude extract was fractionated by open column,which has possibility carried-out fraction rich inresveratrol. The inhibition of DPPH radicals ranged from14.2 to 74.2%, and the total phenolic content ranged from0.1 to 107.0 mg gallic acid equivalents/100 g. Microwaveassistedextraction of grape juice by-products using polarsolvents, such as ethanol and water, provided the best yieldand chemical composition, obtaining extracts rich in flavonoids. In this way, this work has demonstrated theindustrial grape by-products importances, which are a richsource of antioxidants if properly extracted.