Effects of Feeding Purified Zearalenone Contaminated Diets with or without Clay Enterosorbent on Growth, Nutrient Availability, and Genital Organs in Post-weaning Female Pigs

Jiang, S.Z.,Yang, Z.B.,Yang, W.R.,Yao, B.Q.,Zhao, H.,Liu, F.X.,Chen, C.C.,Chi, F. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1

The effects of different levels of natural clay enterosorbent on the growth, nutrient availability, and genital organs of post-weaning female pigs fed with an addition of zearalenone (ZEA) were investigated in the study. A total of thirty-five post-weaning gilts ($L{\times}Y{\times}D$) with an average body weight of 12.36${\pm}$1.46 kg were used in the test. The gilts were raised individually in metabolism cages and fed a corn-soybean meal-whey basal diet with an addition of 0 or 1 mg/kg of ZEA for 24 d with four levels of natural clay enterosorbent added in the feed. The treatments were: i) control; ii) control+2.5 g/kg clay; iii) control+1 mg/kg ZEA; iv) control+1 mg/kg ZEA+1.25 g/kg clay; v) control+1 mg/kg ZEA+2.5 g/kg clay; vi) control+1 mg/kg ZEA+5.0 g/kg clay; vii) control+1 mg/kg ZEA +10 g/kg clay. Pigs fed diets contaminated with additional purified ZEA had significantly reduced apparent digestibility of crude protein (CP), gross energy (GE) and apparent metabolic rate of GE (ME/GE, p<0.05) without changes of net protein utilization (NPU, p>0.05). Final body weight, average daily gain (ADG), average daily feed intake (ADFI), vulva length, vulva width, vulva area, relative weights of genital organ and proliferative changes of the ovary tissues in gilts fed ZEA-contaminated diet were increased (p<0.05) compared to the gilts fed the control diet. Addition of natural clay enterosorbent in the ZEA-contaminated diet showed a positive protection effect on ZEA feeding, and the protection was increased linearly or quadratically as clay content increased. However, in pigs fed a diet with clay alone at 2.5 g/kg level there was no significant impact (p>0.05) on all the parameters as compared to the control. It is suggested that feeding ZEA at about 1.0 mg/kg for 24 days might result in a deleterious effect in pigs, and addition of 5 or 10 g clay enterosorbent per kg diet can effectively neutralize the detrimental effects of the ZEA feeding.

Isolation and Characterization of a Novel Agar-Degrading Marine Bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea

( Won Jae Chi ),( Jae Seon Park ),( Min Jung Kwak ),( Jihyun F. Kim ),( Yong Keun Chang ),( Soon Kwang Hong ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.11

An agar-degrading bacterium, designated as strain G7T, was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7T is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed that it shares 95.5%, 90.6%, and 90.0% similarity with the 16S rRNA gene sequences of Catenovulum agarivorans YM01T, Algicola sagamiensis, and Bowmanella pacifica W3-3AT, respectively. Phylogenetic analyses demonstrated that strain G7T formed a distinct monophyletic clade closely related to species of the family Alteromonadaceae in the Alteromonas-like Gammaproteobacteria. The G+C content of strain G7T was 41.12 mol%. The DNA-DNA hybridization value between strain G7T and the phylogenetically closest strain YM01T was 19.63%. The genomes of G7T and YM01T had an average ANIb value of 70.00%. The predominant isoprenoid quinone of this particular strain was ubiquinone-8, whereas that of C. agarivorans YM01T was menaquinone-7. The major fatty acids of strain G7T were Iso-C15:0 (41.47%), Anteiso-C15:0 (22.99%), and C16:1ω7c/iso-C15:0 2-OH (8.85%), which were quite different from those of YM01T. Comparison of the phenotypic characteristics related to carbon utilization, enzyme production, and susceptibility to antibiotics also demonstrated that strain G7T is distinct from C. agarivorans YM01T. Based on its phenotypic, chemotaxonomic, and phylogenetic distinctiveness, strain G7T was considered a novel genus and species in the Gammaproteobacteria, for which the name Gayadomonas joobiniege gen. nov. sp. nov. (ATCC BAA-2321 = DSM25250T = KCTC23721T) is proposed.

Measurement of lipid flux to advance translational research: evolution of classic methods to the future of precision health

Salvador Amadeo F.,Shyu Chi-Ren,Parks Elizabeth J. 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Over the past 70 years, the study of lipid metabolism has led to important discoveries in identifying the underlying mechanisms of chronic diseases. Advances in the use of stable isotopes and mass spectrometry in humans have expanded our knowledge of target molecules that contribute to pathologies and lipid metabolic pathways. These advances have been leveraged within two research paths, leading to the ability (1) to quantitate lipid flux to understand the fundamentals of human physiology and pathology and (2) to perform untargeted analyses of human blood and tissues derived from a single timepoint to identify lipidomic patterns that predict disease. This review describes the physiological and analytical parameters that influence these measurements and how these issues will propel the coming together of the two fields of metabolic tracing and lipidomics. The potential of data science to advance these fields is also discussed. Future developments are needed to increase the precision of lipid measurements in human samples, leading to discoveries in how individuals vary in their production, storage, and use of lipids. New techniques are critical to support clinical strategies to prevent disease and to identify mechanisms by which treatments confer health benefits with the overall goal of reducing the burden of human disease.

Enhanced delivery of liposomes to lung tumor through targeting interleukin-4 receptor on both tumor cells and tumor endothelial cells

Chi, L.,Na, M.H.,Jung, H.K.,Vadevoo, S.M.P.,Kim, C.W.,Padmanaban, G.,Park, T.I.,Park, J.Y.,Hwang, I.,Park, K.U.,Liang, F.,Lu, M.,Park, J.,Kim, I.S.,Lee, B.H. Elsevier Science Publishers 2015 Journal of controlled release Vol.209 No.-

A growing body of evidence suggests that pathological lesions express tissue-specific molecular targets or biomarkers within the tissue. Interleukin-4 receptor (IL-4R) is overexpressed in many types of cancer cells, including lung cancer. Here we investigated the properties of IL-4R-binding peptide-1 (IL4RPep-1), a CRKRLDRNC peptide, and its ability to target the delivery of liposomes to lung tumor. IL4RPep-1 preferentially bound to H226 lung tumor cells which express higher levers of IL-4R compared to H460 lung tumor cells which express less IL-4R. Mutational analysis revealed that C1, R2, and R4 residues of IL4RPep-1 were the key binding determinants. IL4RPep-1-labeled liposomes containing doxorubicin were more efficiently internalized in H226 cells and effectively delivered doxorubicin into the cells compared to unlabeled liposomes. In vivo fluorescence imaging of nude mice subcutaneously xenotransplanted with H226 tumor cells indicated that IL4RPep-1-labeled liposomes accumulate more efficiently in the tumor and inhibit tumor growth more effectively compared to unlabeled liposomes. Interestingly, expression of IL-4R was high in vascular endothelial cells of tumor, while little was detected in vascular endothelial cells of control organs including the liver. IL-4R expression in cultured human vascular endothelial cells was also up-regulated when activated by a pro-inflammatory cytokine tumor necrosis factor-α. Moreover, the up-regulation of IL-4R expression was observed in primary human lung cancer tissues. These results indicate that IL-4R-targeting nanocarriers may be a useful strategy to enhance drug delivery through the recognition of IL-4R in both tumor cells and tumor endothelial cells.

Estrogen, Body Weight, and Appetite

Bond, Eleanor F.,Deechakawan, Wimon,Chung, Shih-Chi The Research Institute of Nursing Science Seoul Na 2005 간호학의 지평 Vol.2 No.1

Obesity rates are increasing worldwide, associated with excess acute and chronic disease risk. In most countries, obesity rates among women exceed rates in men, particularly during the post menopausal years. Many factors affect body weight and appetite, including age, metabolic rate, physical activity level, stress, cultural factors, socioeconomic status, health status and health literacy, diet composition, attitudes, and beliefs. Gender affects appetite and body weight indirectly by altering factors contributing to food choice. However, there is emerging evidence that gender affects appetite and body weight directly, altering the physiological control systems regulating appetite. The follicular menstrual cycle phase (estrogen-rich) is associated with relative suppression of appetite. Lower estrogen levels are associated with increased food intake, body weight gain, and altered body fat distribution in humans and animals. This paper reviews the linkages between estrogen and appetite regulation. While relationships among appetite, body weight, and gender-linked hormones are complex, research elucidating these interrelationships could lead to development of gender-specific treatment approaches for obesity and appetite dysregulation.

Targeting Heterogeneous Tumors Using a Multifunctional Molecular Prodrug

Sharma, Amit,Lee, Min-Goo,Won, Miae,Koo, Seyoung,Arambula, Jonathan F.,Sessler, Jonathan L.,Chi, Sung-Gil,Kim, Jong Seung American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.39

<P>Reported here is a molecular construct (<B>K1</B>) designed to overcome hurdles associated with delivering active drugs to heterogeneous tumor environments. Construct <B>K1</B> relies on two cancer environment triggers (GSH and H<SUB>2</SUB>O<SUB>2</SUB>) to induce prodrug activation. It releases an active drug form (SN-38) under conditions of both oxidative and reductive stress <I>in vitro</I>. Specific uptake of <B>K1</B> in COX-2 positive aggressive colon cancer cells (SW620 and LoVo) was seen, along with enhanced anticancer activity compared with the control agent SN-38. These findings are attributed to environmentally triggered drug release, as well as simultaneous scavenging of species giving rise to intracellular redox stress. <B>K1</B> serves to downregulate various cancer survival signaling pathways (AKT, p38, IL-6, VEGF, and TNF-α) and upregulate an anti-inflammatory response (IL-10). Compared with SN-38 and DMSO as controls, <B>K1</B> also displayed an improved <I>in vivo</I> therapeutic efficacy in a xenograft tumor regrowth model with no noticeable systematic toxicity at the administrated dose. We believe that the strategy described here presents an attractive approach to addressing solid tumors characterized by intratumoral heterogeneity.</P> [FIG OMISSION]</BR>

Zearalenone Altered the Serum Hormones, Morphologic and Apoptotic Measurements of Genital Organs in Post-weaning Gilts

Chen, X.X.,Yang, C.W.,Huang, L.B.,Niu, Q.S.,Jiang, Shuzhen,Chi, F. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.2

The present study was aimed at investigating the adverse effects of dietary zearalenone (ZEA) (1.1 to 3.2 mg/kg diet) on serum hormones, morphologic and apoptotic measurements of genital organs in post-weaning gilts. A total of twenty gilts ($Landrace{\times}Yorkshire{\times}Duroc$) weaned at 21 d with an average body weight of $10.36{\pm}1.21kg$ were used in the study. Gilts were fed a basal diet with an addition of 0, 1.1, 2.0, or 3.2 mg/kg purified ZEA for 18 d ad libitum. Results showed that 3.2 mg/kg ZEA challenged gilts decreased (p<0.05) the serum levels of luteinizing hormone, however, serum levels of prolactin in gilts fed the diet containing 2.0 mg/kg ZEA or more were increased (p<0.05) compared to those in the control. Linear effects on all tested serum hormones except progesterone were observed as dietary ZEA levels increased (p<0.05). Gilts fed ZEA-contaminated diet showed increase (p<0.05) in genital organs size, hyperplasia of submucosal smooth muscles in the corpus uteri in a dose-dependent manner. However, the decreased numbers of follicles in the cortex and apoptotic cells in the ovarian were observed in gilts treated with ZEA in a dose-dependent manner. Degeneration and structural abnormalities of genital organs tissues were also observed in the gilts fed diet containing 1.1 mg/kg ZEA or more. Results suggested that dietary ZEA at 1.1 to 3.2 mg/kg can induce endocrine disturbance and damage genital organs in post-weaning gilts.

Phage Application for the Protection from Acute Hepatopancreatic Necrosis Disease (AHPND) in Penaeus vannamei

Jun, Jin Woo,Han, Jee Eun,Giri, Sib Sankar,Tang, Kathy F. J.,Zhou, Xiaohui,Aranguren, Luis Fernando,Kim, Hyoun Joong,Yun, Saekil,Chi, Cheng,Kim, Sang Guen,Park, Se Chang Springer-Verlag 2018 Indian journal of microbiology Vol.58 No.1

<P>Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has been one of the most problematic diseases in marine shrimp aquaculture throughout Southeast Asia and Latin America. To evaluate the effectiveness of a bacteriophage (phage) treatment for AHPND, a series of bioassays were carried out in a marine shrimp (Penaeus vannamei) model using an AHPND-V. parahaemolyticus strain that is highly pathogenic to shrimp. We monitored the mortality and histopathological changes during phage treatment. Shrimps treated with phage prophylaxis and phage therapy displayed significant protection from AHPND and survived a lethal bacterial challenge.</P>

산사태 취약성 분석을 위한 GIS 기반 확률론적 추정 모델과 모수적 모델의 적용

박노욱(No-Wook Park),지광훈(Kwang-Hoon Chi),Chang-Jo F. Chung,권병두(Byung-Doo Kwon) 대한자원환경지질학회 2005 자원환경지질 Vol.38 No.1

산사태 취약성 분석을 위해 적용된 기존 GIS 기반 확률론적 공간 통합 모델은 범주형과 연속형 자료와 같이 서로 다른 형태의 자료의 처리를 위한 이론적 배경과 효율적인 방법론을 제시하지 못하였다. 이 논문에서는 우도비의 틀안에서 연속형 자료를 직접적으로 사용할 수 있도록 비모수적 경험적 추정 모델과 모수적 예측적 판별 분석 모델을 적용하였다. 그리고 유사율과 예측비율곡선을 계산함으로써 두 모델을 정량적으로 비교하고자 하였다. 제안 모델을 비교하기 위해 1998년 여름 산사태로 많은 피해를 입은 장흥 지역과 보은 지역을 대상으로 사례연구를 수행하였다. 장흥 지역에서는 두 모델이 유사한 예측 능력을 나타내었으나, 보은 지역에서는 모수적 예측적 판별 분석 모델이 보다 높은 예측 능력을 나타내었다. 결론적으로 제안한 두 모델은 산사태 취약성 분석을 위한 연속형 자료 표현에 효율적 으로 적용될 수 있으며, 두 모델이 개별적인 연속형 자료 표현의 특성을 가지고 있기 때문에 다른 사례 연구를 통한 검증 작업이 병행되어야 할 것으로 생각된다. Traditional GIS-based probabilistic spatial data integration models for landslide susceptibility analysis have failed to provide the theoretical backgrounds and effective methods for integration of different types of spatial data such as categorical and continuous data. This paper applies two spatial data integration models including non-parametric empirical estimation and parametric predictive discriminant analysis models that can directly use the original continuous data within a likelihood ratio framework. Similarity rates and a prediction rate curve are computed to quantitatively compare those two models. To illustrate the proposed models, two case studies from the Jangheung and Boeun areas were carried out and analyzed. As a result of the Jangheung case study, two models showed similar prediction capabilities. On the other hand, in the Boeun area, the parametric predictive discriminant analysis model showed the better prediction capability than that from the non-parametric empirical estimation model. In conclusion, the proposed models could effectively integrate the continuous data for landslide susceptibility analysis and more case studies should be carried out to support the results from the case studies, since each model has a distinctive feature in continuous data representation.

2-Methoxy-1,4-naphthoquinone (MNQ) regulates cancer key genes of MAPK, PI3K, and NF-κB pathways in Raji cells

Wong, Teck Yew,Menaga, Subramaniam,Huang, Chi-Ying F.,Ho, Siong Hock Anthony,Gan, Seng Chiew,Lim, Yang Mooi Korea Genome Organization 2022 Genomics & informatics Vol.20 No.1

2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.