철근콘크리트 耐震壁의 强度와 變形

金銀謙 서울産業大學校 1994 논문집 Vol.39 No.1

2차원 재료 모델을 기본으로 RC 비선형 유한요소 프로그램「WCOMR」이 東京大學의 岡村들에 의해 개발되었다. 본 프로그램은「JCI」에서 선정한 내진벽 실험체를 이용한 검증이 실시되어 그의 유용성이 입증되었으나, 3차원 영향이 큰 내진벽의 해석 및 재하속도에 따른 콘크리트 압축강성 모델의 시간 의존성 문제가 정밀도면에서 지적되었다. 따라서 본 연구에서는「WCOMR」이용하여 해석 가능한 2차원 구조 및 3차원 구조의 한계를 명확히 함과 동시에, 내진벽의 시간의존성 문제를 정량평가하는데 목적을 두고 있다.

이소말토 올리고당의 첨가가 약과의 품질특성에 미치는 영향

김향숙,서월석,김은겸,이화영 충북대학교 교육 ·생활연구소 생활과학연구센터 2002 생활과학연구논총 Vol.6 No.2

This study was to develope low-calorie Yak-kwa by using oligosaccharides instead of honey. Sensory and instrumental textur properties of Yak-kwa were examined. The calorie of oligosaccharide Yak-kwa was 31% lower than honey Yak-kwa. Color of oligosaccharide Yak-kwa was lighter than that of honey Yak-kwa. Results of nstrumental test of texural proporties showed that hardness increased and cutting decreased as soaking time increased without significant differences between oligosaccharide Yak-kwa and honey Yak-kwa. Accepance test by sensoty panel showed no significant differences between oligosaccharid Yak-kwa and honey Yak-kwa.

縱, 橫方向 鐵筋比의 변화가 RC비틀림 部材에 미치는 力學性狀

申鉉默,金銀謙,朴潤濟 成均館大學校 科學技術硏究所 1989 論文集 Vol.40 No.1

지금까지 RC 비틀림 部材에 대한 硏究들은 縱方向 및 橫方向 철근비를 동일하게 하면 최대의 비틀림强度를 얻을 수 있다고 하는 이른바 同一體積比의 原理에 입각한 것이 대부분이다. 그러나, 최근 RC 構造物의 설계법이 許容應力度 設計法에서 强度設計法 내지는 限界狀態設計法으로 轉換되어 가는 추세에 있음을 고려할 때, RC 비틀림부재의 縱. 橫方向 철근비를 달리하였을 경우 이들 철근이 각각 RC 비틀림부재의 비틀림강도 및 QUSGUDD 미치는 영향을 상세히 파악한다는 것은 매우 의미있는 일이다. 본 연구는 이와 같은 관점에서 縱. 橫方向의 철근비를 각각 달리한 7個의 RC 部材를 제작하여 載荷實驗을 실시, 이들이 RC 부재의 비틀림강도 및 변형에 미치는 영향을 實驗的으로 고찰하는데 目的을 둔 것이다. Most previous studies for the RC torisional members are based on the equal volume principle. Then, current design method requires not only the torsional strengths but also the serviceability on the torsional members. So, this study aimed to investigate the deformation characteristics as well as the torsional strengths on the R.C. torsional members by the variance of reinforcement ratio through the experiment with set-en test beams.

강교의 도장방식에 따른 안전수명간 생애주기비용분석

조선규,한상철,김은겸 한국산업안전학회 2002 한국안전학회지 Vol.17 No.2

Life Cycle Cost analysis technique is introduced to evaluate cost-effectiveness of two paint systems of steel bridges. The systems are a conventional paint system and a galvanized paint system. The all costs during safe life such as initial cost, repainting costs, disposal costs are considered for the life cycle cost analysis. The NIST model is used and BridgeLOC 1.0 developed by the NIST is utilized as the life cycle cost analysis tool. It is concluded that, in spite of expensive initial cost, the durable paints system may be cost-effective compared with conventional paint system.

Conversion of glioma cells to glioma stem-like cells by angiocrine factors

Kim, Jun-Kyum,Jeon, Hye-Min,Jeon, Hee-Young,Oh, Se-Yeong,Kim, Eun-Jung,Jin, Xiong,Kim, Se-Hoon,Kim, Sung-Hak,Jin, Xun,Kim, Hyunggee Elsevier 2018 Biochemical and biophysical research communication Vol.496 No.4

<P><B>Abstract</B></P> <P>Glioma stem-like cells (GSCs) contribute to tumor initiation, progression, and therapeutic resistance, but their cellular origin remains largely unknown. Here, using a stem/progenitor cell-fate tracking reporter system in which eGFP is expressed by promoter of <I>OCT4</I> that is activated in stem/progenitor cells, we demonstrate that eGFP-negative glioma cells (GCs) became eGFP-positive-GCs in both <I>in vitro</I> cultures and <I>in vivo</I> xenografts. These eGFP-positive-GCs exhibited GSC features and primarily localized to the perivascular region in tumor xenografts, similar to the existence of OCT4-expressing GCs in the perivascular region of human glioblastoma specimens. Angiocrine factors, including nitric oxide (NO), converted eGFP-negative-GCs into eGFP-positive-GCs. Mechanistically, NO signaling conferred GSC features to GCs by increasing OCT4 and NOTCH signaling via ID4. NO signaling blockade and a suicide gene induction prevented tumorigenicity with a decrease in eGFP-positive-GCs in the perivascular region. Taken together, our results reveal the molecular mechanism underlying GSCs generation by cancer cell dedifferentiation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Glioma cells are converted to glioma stem-like cells in <I>in vivo</I> xenografts. </LI> <LI> Angiocrine factors convert glioma cells to glioma stem-like cells. </LI> <LI> Nitric oxide regulates ID4 and OCT4 expression in glioma stem-like cells. </LI> <LI> Blockade of nitric oxide signaling prevents tumor progression. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

고로슬래그미분말을 사용한 콘크리트의 염화물이온에 의한 철근부식 저항성 연구

김은겸(Eun-Kyum Kim),김진근(Jin-Keun Kim),이동혁(Dong-Hyuk Lee),김영웅(Young-Ung Kim),김용철(Yong-Chul Kim) 한국콘크리트학회 2004 콘크리트학회논문집 Vol.16 No.1

Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles

Lee, Eun-Young,Choi, Do-Young,Kim, Dae-Kyum,Kim, Jung-Wook,Park, Jung Ok,Kim, Sungjee,Kim, Sang-Hyun,Desiderio, Dominic M.,Kim, Yoon-Keun,Kim, Kwang-Pyo,Gho, Yong Song WILEY-VCH Verlag 2009 Proteomics Vol.9 No.24

<P>Although archaea, Gram-negative bacteria, and mammalian cells constitutively secrete membrane vesicles (MVs) as a mechanism for cell-free intercellular communication, this cellular process has been overlooked in Gram-positive bacteria. Here, we found for the first time that Gram-positive bacteria naturally produce MVs into the extracellular milieu. Further characterizations showed that the density and size of Staphylococcus aureus-derived MVs are both similar to those of Gram-negative bacteria. With a proteomics approach, we identified with high confidence a total of 90 protein components of S. aureus-derived MVs. In the group of identified proteins, the highly enriched extracellular proteins suggested that a specific sorting mechanism for vesicular proteins exists. We also identified proteins that facilitate the transfer of proteins to other bacteria, as well to eliminate competing organisms, antibiotic resistance, pathological functions in systemic infections, and MV biogenesis. Taken together, these observations suggest that the secretion of MVs is an evolutionally conserved, universal process that occurs from simple organisms to complex multicellular organisms. This information will help us not only to elucidate the biogenesis and functions of MVs, but also to develop therapeutic tools for vaccines, diagnosis, and antibiotics effective against pathogenic strains of Gram-positive bacteria.</P>

Induction of Laccase Production and Analysis of Laccase Isozymes in Trichophyton rubrum LKY-7

( Eun Seon Shin ),( Sung Kyum Kim ),( Yong Ho Kim ),( Yu Seok Jeong ),( Hyun Chae Jung ),( Seur Kee Park ),( Jung Ho Kim ),( Hoon Kim ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.2

Trichophyton rubrum LKY-7 is a wood-degrading fungus, which has been isolated from hardwood chip piles. T. rubrum LKY-7 showed better growth in glucose-peptone medium (GPM) than in basal medium (BM) with rotary shaking, and mycelial growth was facilitated by the presence of wood powder. Aeration significantly increased the production of laccase in GPM (6 to 17 U/mL), and the addition of wood powder to the medium greatly increased it to 78.5 U/mL. T. rubrum LKY-7 produced no detectable amounts of lignin peroxidase, manganese peroxidase, cellulose, or xylanase under the prescribed culture conditions. A three-step chromatographic separation of the laccase indicated that at least two kinds of laccase isozymes were produced by the fungus. Activity staining of the non-boiled enzyme samples after SDS-PAGE revealed different active protein band patterns for the laccase produced in the absence or presence of the wood powder. A difference in the isozyme pattern was also observed on the activity stained gel after native PAGE. These results suggest that wood powder not only increases the production of laccase but also changes the kinds of isozymes produced by T. rubrum LKY-7. No laccase active bands were detected when the enzyme preparations were boiled for 60 s or longer, indicating that the laccase isozymes are heat-labile.

Subacute toxicity and toxicokinetics study of DHP107, an oral paclitaxel formulation with once-weekly dosing in mice

Kim, Ji-Young,Lee, Sunyeong,Kim, Yongbum,Jeong, Eun Ju,Lee, In-Hyun,Son, Min-Hee,Lee, Jae Young,Kim, Sang Kyum,Moon, Kyoung-Sik Elsevier 2019 Regulatory toxicology and pharmacology Vol.103 No.-

<P><B>Abstract</B></P> <P>DHP107, an oral formulation of paclitaxel, is effectively and systemically absorbed in intestinal endothelial cells. Although the <I>in vivo</I> efficacy of DHP107 has been reported, the potential toxicity of DHP107 has not been evaluated. Therefore, this study was conducted to evaluate the toxicity and toxicokinetics of DHP107 orally administered to ICR mice at 25, 50, and 100 mg/kg via once-weekly dosing for six weeks. DHP107-related clinical signs were observed in both sexes at 100 mg/kg. There were significant increases in the number of platelets and percentages of reticulocytes and basophils in male mice. Also in males, there was a significant decrease in the absolute and relative weights of testes, epididymides, kidneys, and heart. Relative spleen weights were significantly increased in males treated with doses ≥50 mg/kg which had histopathological correlates. These changes were reversible after a two-week recovery period with the exception of the findings in the reproductive organs. Systemic exposure to paclitaxel increased with DHP107 doses in single and multiple dosing with no marked differences between sexes. In conclusion, the target organs were determined to be the reproductive and hematopoietic organs in male mice, suggesting of sex difference and the NOAEL of DHP107 was established to be < 25 mg/kg for males and 50 mg/kg for females.</P> <P><B>Highlights</B></P> <P> <UL> <LI> DHP107 is an oral cancer therapy containing paclitaxel as a single active ingredient. </LI> <LI> The target organs of DHP107 were reproductive and hematopoietic organs. </LI> <LI> The systemic exposure was increased dose-dependently. </LI> <LI> The NOAEL of DHP107 was under 25 mg/kg for males, and 50 mg/kg for females. </LI> </UL> </P>

Twelve unrecorded UV-resistant bacterial species isolated in 2020

Kim, Ju-Young,Maeng, Soohyun,Park, Yuna,Lee, Sang Eun,Han, Joo Hyun,Cha, In-Tae,Lee, Ki-eun,Kim, Myung Kyum The National Institute of Biological Resources 2021 Journal of species research Vol.10 No.4

In 2020, a total of 12 bacterial strains were isolated from soil after a comprehensive investigation of indigenous prokaryotic species in Korea. It was determined that each strain belonged to independent and predefined bacterial species, with high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species. This study identified four families in the phylum Actinobacteria, two families in the phylum Proteobacteria, one family in the phylum Bacteroidetes one family in the phylum Firmicutes; and four species in the family Nocardiaceae, two species in the family Nocardioidaceae, one species in the family Cellulomonadaceae, one species in the family Hymenobacter, one species in the family Methylobacteriaceae, one species in the family Microbacteriaceae, one species in the family Bacillaceae and one species in the family Sphingomonadaceae. There is no official report of these 12 species in Korea, so they are described as unreported bacterial species in Korea in this study. Gram reaction, basic biochemical characteristics, colony, and cell morphology are included in the species description section.