Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

북한 제2경제권에 대하여

정유진 연세대학교 통일연구원 1997 통일연구 Vol.1 No.1

Forest Soil Metagenome Gene Cluster Involved in Antifungal Activity Expression in Escherichia coli

Chung, Eu Jin,Lim, He Kyoung,Kim, Jin-Cheol,Choi, Gyung Ja,Park, Eun Jin,Lee, Myung Hwan,Chung, Young Ryun,Lee, Seon-Woo American Society for Microbiology 2008 Applied and environmental microbiology Vol.74 No.3

<B>ABSTRACT</B><P>Using two forest soils, we previously constructed two fosmid libraries containing 113,700 members in total. The libraries were screened to select active antifungal clones using <I>Saccharomyces cerevisiae</I> as a target fungus. One clone from the Yuseong pine tree rhizosphere soil library, pEAF66, showed <I>S. cerevisiae</I> growth inhibition. Despite an intensive effort, active chemicals were not isolated. DNA sequence analysis and transposon mutagenesis of pEAF66 revealed 39 open reading frames (ORFs) and indicated that eight ORFs, probably in one transcriptional unit, might be directly involved in the expression of antifungal activity in <I>Escherichia coli</I>. The deduced amino acid sequences of eight ORFs were similar to those of the core genes encoding type II family polyketide synthases, such as the acyl carrier protein (ACP), ACP synthases, aminotransferase, and ACP reductase. The gene cluster involved in antifungal activity was similar in organization to the putative antibiotic production locus of <I>Pseudomonas putida</I> KT2440, although we could not select a similar active clone from the KT2440 genomic DNA library in <I>E. coli</I>. ORFs encoding ATP binding cassette transporters and membrane proteins were located at both ends of the antifungal gene cluster. Upstream ORFs encoding an IclR family response regulator and a LysR family response regulator were involved in the positive regulation of antifungal gene expression. Our results suggested the metagenomic approach as an alternative to search for novel antifungal antibiotics from unculturable soil bacteria. This is the first report of an antifungal gene cluster obtained from a soil metagenome using <I>S. cerevisiae</I> as a target fungus.</P>

Nitrospirillum irinus sp. nov., a diazotrophic bacterium isolated from the rhizosphere soil of Iris and emended description of the genus Nitrospirillum

Chung, Eu Jin,Park, Tae Soon,Kim, Kyung Hyun,Jeon, Che Ok,Lee, Hae-In,Chang, Woo-Suk,Aslam, Zubair,Chung, Young Ryun Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3

Production of a Phytotoxic Compound, 3-Phenylpropionic Acid by a Bacterial Endophyte, Arthrobacter humicola YC6002 Isolated from the Root of Zoysia japonica

Chung, Eu-Jin,Park, Joo-Hwang,Park, Tae-Soon,Ahn, Jong-Woong,Chung, Young-Ryun The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3

An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

Sphingomonas oryziterrae sp. nov. and Sphingomonas jinjuensis sp. nov. isolated from rhizosphere soil of rice (Oryza sativa L.).

Chung, Eu Jin,Jo, Eun Ju,Yoon, Hwan Sik,Song, Geun Cheol,Jeon, Che Ok,Chung, Young Ryun Society for General Microbiology 2011 International journal of systematic and evolutiona Vol.61 No.10

<P>Two Gram-reaction-negative, yellow-orange-pigmented, rod-shaped bacterial strains, designated YC6722(T) and YC6723(T), were isolated from rhizosphere soil samples collected from rice fields in Jinju, Korea. Strains YC6722(T) and YC6723(T) grew optimally at 25-30 °C and at pH 7.0-8.5. Phylogenetic analyses of 16S rRNA gene sequences showed that strain YC6722(T) was most closely related to Sphingomonas jaspsi TDMA-16(T) (96.6???% sequence similarity) and strain YC6723(T) was related most closely to Sphingomonas aquatilis JSS7(T) (96.9???%). The two strains contained ubiquinone-10 (Q-10) as the major respiratory quinone system and sym-homospermidine as the major polyamine. The G+C contents of the genomic DNA of strains YC6722(T) and YC6723(T) were 63.3 and 61.0 mol%, respectively. The major fatty acid was C(18???:???1)??7c. The polar lipids detected in the two strains were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, phosphatidyldimethylethanolamine and other unknown lipids. On the basis of their phylogenetic positions, and their biochemical and phenotypic characteristics, strains YC6722(T) and YC6723(T) represent two novel species of the genus Sphingomonas, for which the names Sphingomonas oryziterrae sp. nov. (???=???KCTC 22476(T) ???=???DSM 21455(T)) and Sphingomonas jinjuensis sp. nov. (KCTC 22477(T) ???=???DSM 21457(T)) are proposed.</P>

Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice

Chung, Eu Jin,Hossain, Mohammad Tofajjal,Khan, Ajmal,Kim, Kyung Hyun,Jeon, Che Ok,Chung, Young Ryun The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.2

Biological control of major rice diseases has been attempted in several rice-growing countries in Asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. Two novel endophytic Bacillus species, designated strains YC7007 and $YC7010^T$, with antimicrobial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at Jinju, Korea, and their multifunctional activities were analyzed. Strain YC7007 inhibited mycelial growth of major rice fungal pathogens strongly in vitro. Bacterial blight and panicle blight caused by Xanthomonas oryzae pv. oryzae (KACC 10208) and Burkholderia glumae (KACC 44022), respectively, were also suppressed effectively by drenching a bacterial suspension ($10^7cfu/ml$) of strain YC7007 on the rhizosphere of rice. Additionally, strain YC7007 promoted the growth of rice seedlings with higher germination rates and more tillers than the untreated control. The taxonomic position of the strains was also investigated. Phylogenetic analyses based on 16S rRNA gene sequences indicated that both strains belong to the genus Bacillus, with high similarity to the closely related strains, Bacillus siamensis KACC $15859^T$ (99.67%), Bacillus methylotrophicus KACC $13105^T$ (99.65%), Bacillus amyloliquefaciens subsp. plantarum KACC $17177^T$ (99.60%), and Bacillus tequilensis KACC $15944^T$ (99.45%). The DNA-DNA relatedness value between strain $YC7010^T$ and the most closely related strain, B. siamensis KACC $15859^T$ was $50.4{\pm}3.5%$, but it was $91.5{\pm}11.0%$ between two strains YC7007 and $YC7010^T$, indicating the same species. The major fatty acids of two strains were anteiso-$C_{15:0}$ and iso $C_{15:0}$. Both strains contained MK-7 as a major respiratory quinone system. The G+C contents of the genomic DNA of two strains were 50.5 mol% and 51.2 mol%, respectively. Based on these polyphasic studies, the two strains YC7007 and $YC7010^T$ represent novel species of the genus Bacillus, for which the name Bacillus oryzicola sp. nov. is proposed. The type strain is $YC7010^T$ (= KACC $18228^T$). Taken together, our findings suggest that novel endophytic Bacillus strains can be used for the biological control of rice diseases.

<i>Gynuella sunshinyii</i> gen. nov., sp. nov., an antifungal rhizobacterium isolated from a halophyte, <i>Carex scabrifolia</i> Steud

Chung, Eu Jin,Park, Jeong Ae,Jeon, Che Ok,Chung, Young Ryun International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.3

<P>An antifungal bacterial strain, designated YC6258<SUP>T</SUP>, was isolated from the rhizosphere of a halophyte (<I>Carex scabrifolia</I> Steud.) growing in a tidal flat area of Namhae Island, Korea. Cells of the strain were Gram-stain-negative, facultatively anaerobic, moderately halophilic, rod-shaped and motile by a single polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC6258<SUP>T</SUP> formed a phyletic lineage distinct from members of the most closely related genera, <I>Saccharospirillum</I> and <I>Reinekea</I>, with less than 91.2 % sequence similarities. The major cellular fatty acids were C<SUB>18 : 1</SUB>ω7<I>c</I>, C<SUB>16 : 0</SUB> and Summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I>/ C<SUB>16 : 1</SUB>ω6<I>c</I>). The quinone system of strain YC6258<SUP>T</SUP> consisted mainly of ubiquinone Q-8. The polar lipid profile exhibited phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unknown lipids. The DNA G+C content was 48.9 mol%. Based on the phylogenetic and phenotypic characteristics, strain YC6258<SUP>T</SUP> should be classified as a representative of a novel species in a novel genus for which the name <I>Gynuella sunshinyii</I> gen. nov., sp. nov. is proposed. The type strain is YC6258<SUP>T</SUP> (KCCM 43015<SUP>T</SUP> = NBRC 109345<SUP>T</SUP>).</P>

Effect of Autoclave and Gamma-Irradiation Treatmentson Allergenicity of Pork Ham

Seo-Jin KIM,Koth-Bong-Woo-Ri KIM,So-Young LEE,Eu-Jin SONG,So-Young YOON,So-Jeong LEE,Chung-Jo LEE,Jin-Gyu PARK,Ju-Woon LEE,Myung-Woo BYUN,Kyu-Earn KIM,Dong-Hyun AHN 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.4

커피박 추출물이 간고등어의 저장성과 품질에 미치는 영향

송유진(Eu Jin Song),김진율(Jin Yul Kim),이소영(So Young Lee),김꽃봉우리(Koth Bong Woo Ri Kim),김서진(Seo Jin Kim),윤소영(So Young Yoon),이소정(So Jeong Lee),이청조(Chung Jo Lee),안동현(Dong Hyun Ahn) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.6

커피박의 항산화능을 확인하고 커피박의 새로운 용도를 개발하고자 지질산화로 인한 선도저하가 문제시 되는 간고등어의 제조에 커피박 열수 추출물을 천연항산화제로 이용하였다. 먼저, 커피박 열수 추출물의 항산화 활성을 측정하기 위해 DPPH radical 소거능을 측정한 결과, 4~0.4 ㎎/mL 농도에서 약 92%의 높은 라디칼 소거능을 유지하였다. 항산화 활성을 확인한 커피박 열수 추출물 5, 10 및 15%를 염수에 첨가하여 간고등어를 제조한 후, 4℃에 15일간 저장하면서 산화도, VBN, pH 및 관능적 특성의 변화를 측정하였다. 그 결과, 15% 커피박 열수 추출물 처리구가 저장기간 내내 무처리구에 비해 낮은 산화도 값을 나타내었으며, VBN 함량은 10일까지는 시험구간의 차이를 보이지 않다가 급격한 산화가 시작된 저장 15일에 유의적으로 낮은 값을 보였다. pH는 저장기간이 증가할수록 증가하는 경향을 보였으나 실험구간의 유의적인 차이는 나타나지 않았다. 저장 0일과 5일에 관능평가를 실시한 결과, 향, 맛 그리고 전체적인 호감도 항목에서 10과 15% 커피박 열수 추출물 처리구가 높은 값을 보였다. 이상의 결과를 통해 15% 커피박 열수 추출물 처리를 통해 간고등어의 산화도가 억제됨을 확인하였으며 동시에 관능적 품질이 증진됨을 알 수 있었다. 따라서 커피박열수 추출물을 간고등어와 같이 빠른 지질산화로 인한 저장성 및 품질저하를 유발하는 식품에 천연 항산화제로써 사용할 수 있을 것으로 사료된다. To develop a novel application for roasted ground coffee residue, hot water extract of roasted ground coffee residue was used in manufacturing salted mackerels. First, DPPH radical scavenging effect of roasted ground coffee residue extract was measured. As a result, roasted ground coffee residue extract showed high activity of about 92% in the concentration of 0.4 to 4 ㎎/mL. Thereafter, the salted mackerel was treated with 5, 10 and 15% of roasted ground coffee residue extract and its shelf-life and quality was investigated. The salted mackerels treated with 15% roasted ground coffee residue extract showed significantly low TBARS and VBN as compared to the control. In sensory evaluation, the salted mackerels treated with 10 and 15% roasted ground coffee residue extracts scored higher as compared to the control. In conclusion, roasted ground coffee residue extract increased the shelf-life of salted mackerel and improved the sensual quality by inhibiting lipid oxidation.